盆栽条件下暗褐网柄牛肝菌人工菌塘及其子实体的培养

在土壤中添加促进暗褐网柄牛肝菌(Phlebopus portentosus)菌丝及其宿主咖啡生长的营养液,用暗褐网柄牛肝菌纯培养种接种盆栽件下的咖啡苗根系,结果表明:92.5%以上的小粒咖啡(Coffea arabica)苗形成菌根,菌根上外延菌丝向根尖、侧根及根系周围的土壤延伸生长,与土粒相互交结形成菌塘。接种20天后,菌塘中子实体紧贴咖啡苗茎基或于咖啡苗株间生长,共生长162个子实体,发育成熟52个。子实体菌柄基部菌索与咖啡苗主根表面菌套连接。

一种松茸人工菌塘的建立方法

通过母种分离培养、液体菌种制备、菌丝土壤纯培养物制备和野外菌塘诱导建立等过程,建立一种松茸人工菌塘的制作方法,以促进松茸出产林地菌塘的形成,为人工林地驯化栽培松茸提供一种更有效的途径。

Research Progress on Artificial Cultivation of Trhicoloma Matsutake and Theory of the Shiro in Changbai Mountains

Study on artificial cultivation of Tricholoma matsutake carried through by the present research group in Changbai Mountains in recent years was briefly reviewed in the paper, and then, the signifycance of cultivation. Mechanism of formation, hiberarchy, grouwth cycle and suitable living environment, were summarized.