The meso-tetra(α,α,α,α-O-phenylacetylbenzene)porphyrin was used as a complete antigen to elicit monoclonal antibody 1F2 through the immunization and cell fusion techniques. The antibody could combine with Mn porph...The meso-tetra(α,α,α,α-O-phenylacetylbenzene)porphyrin was used as a complete antigen to elicit monoclonal antibody 1F2 through the immunization and cell fusion techniques. The antibody could combine with Mn porphyrin and showed a haloperoxidase activity. The affinities of the antibody for antigen and Mn porphyrin were 4 98×10 -5 mol/L and 7 63×10 -5 mol/L, respectively. When the antibody-Mn porphyrin complex was used to catalyze the halogenation reaction, the Michaelis constant and reactive rate constant κ cat for H 2O 2 were 5 127 0×10 -3 mol/L and 0 483 4 s -1, respectively. The enzyme exchange number κ cat/K m=94 285 1 mol/(L·s).展开更多
近日,超声医学科邱逦教授带领的医工交叉学科研究团队在材料领域期刊Advanced Materials发表了题为"Modulating Electron Transfer in Vanadium-based Artificial Enzymes for Enhanced ROS-Catalysis and Disinfection"的封...近日,超声医学科邱逦教授带领的医工交叉学科研究团队在材料领域期刊Advanced Materials发表了题为"Modulating Electron Transfer in Vanadium-based Artificial Enzymes for Enhanced ROS-Catalysis and Disinfection"的封面论文。该论文揭示了一种利用独特的Zn-O-V桥构筑钒基的新型HPO人造酶材料,并验证其具有与万古霉素相当的抗菌性能,能够有效促进创面新生血管形成、降低组织修复过程中的炎性反应,为人工酶的设计提供新的研究思路。展开更多
文摘The meso-tetra(α,α,α,α-O-phenylacetylbenzene)porphyrin was used as a complete antigen to elicit monoclonal antibody 1F2 through the immunization and cell fusion techniques. The antibody could combine with Mn porphyrin and showed a haloperoxidase activity. The affinities of the antibody for antigen and Mn porphyrin were 4 98×10 -5 mol/L and 7 63×10 -5 mol/L, respectively. When the antibody-Mn porphyrin complex was used to catalyze the halogenation reaction, the Michaelis constant and reactive rate constant κ cat for H 2O 2 were 5 127 0×10 -3 mol/L and 0 483 4 s -1, respectively. The enzyme exchange number κ cat/K m=94 285 1 mol/(L·s).
文摘近日,超声医学科邱逦教授带领的医工交叉学科研究团队在材料领域期刊Advanced Materials发表了题为"Modulating Electron Transfer in Vanadium-based Artificial Enzymes for Enhanced ROS-Catalysis and Disinfection"的封面论文。该论文揭示了一种利用独特的Zn-O-V桥构筑钒基的新型HPO人造酶材料,并验证其具有与万古霉素相当的抗菌性能,能够有效促进创面新生血管形成、降低组织修复过程中的炎性反应,为人工酶的设计提供新的研究思路。
