Chronic hepatitis C is a leading cause of morbidity and mortality, mainly related to fibrosis/cirrhosis and portal hypertension. Direct antiviral agents are highly effective and safe and can now cure > 90% of the p...Chronic hepatitis C is a leading cause of morbidity and mortality, mainly related to fibrosis/cirrhosis and portal hypertension. Direct antiviral agents are highly effective and safe and can now cure > 90% of the patients. Sustained viral response (SVR) after interferon-based regimens has been associated with improvement in liver function, fibrosis and portal hypertension in a significant proportion of patients, although a point of no return seems to exist from which viral elimination is no longer capable of preventing portal hypertension progression and liver decompensation. Indeed, although SVR is associated with improvement of hepatic venous pressure gradients and therefore a decreased risk of de novo esophageal varices, several studies show that viral clearance does not eliminate the risk of variceal progression, liver decompensation and death in patients with pre-established portal hypertension. Although evidence about the effects of direct antiviral agents (DAAs) on clinically significant outcomes is still scarce and with short follow-up, DAAs can decrease the burden of the disease if patients are timely treated before significant fibrosis and portal hypertension develops. Studies with longer follow-up are waited to establish the real magnitude of hepatitis C treatment on portal hypertension. Future studies should also focus on predictors of portal hypertension resolution since it can influence management and avoid unnecessary monitoring展开更多
An optimized recombinant HPV16 E6E7 fusion gene (HPV16 ofE6E7) was constructed according to codon usage for mammalian cell expression, and a mutant of HPV16 ofE6E7 fusion gene (HPV16 omfE6E7) was generated by site-dir...An optimized recombinant HPV16 E6E7 fusion gene (HPV16 ofE6E7) was constructed according to codon usage for mammalian cell expression, and a mutant of HPV16 ofE6E7 fusion gene (HPV16 omfE6E7) was generated by site-directed mutagenesis at L57G, C113R for the E6 protein and C24G, E26G for the E7 protein for HPV16 ofE6E7 [patent pending (CN 101100672)]. The HPV16 omfE6E7 gene constructed in this work not only lost the transformation capability to NIH 3T3 cells and tumorigenicity in SCID mice, but also maintained very good stability and antigenicity. These results suggests that the HPV16 omfE6E7 gene should undergo further study for application as a safe antigen-specific therapeutic vaccine for HPV16-associated tumors.展开更多
Three global datasets, the History Database of the Global Environment (HYDE), Kaplan and Krurnhardt (KK) and Pongratz of reconstructed anthropogenic land cover change (ALCC) were introduced and compared in this ...Three global datasets, the History Database of the Global Environment (HYDE), Kaplan and Krurnhardt (KK) and Pongratz of reconstructed anthropogenic land cover change (ALCC) were introduced and compared in this paper. The HYDE dataset was recon- structed by Goldewijk and his colleagues at the National institute of Public ttealth and the Environment in Netherland, covering the past 12 000 years. The KK dataset was reconstructed by Kaplan and his colleagues, the Soil-Vegetation-Atmosphere Research Group at the Institute of Environmental Engineering in Switzerland, covering the past 8000 years. The Pongratz dataset was reconstructed by Pon- gratz and her colleagues at the Max Planck Institute for Meteorology in Germany, coveting AD 800-1992. The results show that the reconstructed datasets are quite different from each other due to the different methods used. The three datasets all allocated the historical ALCC according to human population density. The main reason causing the differences among the three datasets lies on the different relationships between population density and land use used in each reconstructed dataset. The KK dataset is better than the other two datasets for two important reasons. First, it used the nonlinear relationship between population density and land use, while the other two used the linear relationship. Second, Kaplan and his colleagues adopted the technological development and intensification parameters and considered the wood harvesting and the long-term fallow area resulted from shifting cultivation, which were neglected in the recon- structions of the other two datasets. Therefore, the KK dataset is more suitable as one of the anthropogenic forcing fields for climate simulation over the past two millennia that is recently concerned by two projects, the National Basic Research Program and the Strategic and Special Frontier Project of Science and Technology of the Chinese Academy of Sciences.展开更多
Intraspecific variability in Schizaphis graminum (Rondani) was studied in 2002-2005. Aphid clones were sampled from sorghum field at the Kuban Experimental Station (Krasnodar region, Northern Caucasus, Russia) in ...Intraspecific variability in Schizaphis graminum (Rondani) was studied in 2002-2005. Aphid clones were sampled from sorghum field at the Kuban Experimental Station (Krasnodar region, Northern Caucasus, Russia) in June (active greenbug migration to the field), July (high rate of the population increasing), and August (greenbug abundance decreasing) and at the end of September or in the beginning of October (appearance of sexual males and females). Damage rating was estimated for two plant sets composed of three sorghum differentials. Set A contains Deer (gene for resistance Sgr4), Sarvasi (Sgrl + Sgr2) and Capbam (Sgrl2). Set B contains Shallu (Sgr3), Sorgogradskoe (Sgr5) and Durra Belaya (Sgr5 + Sgr6). To estimate variability in greenbug subpopulations criteria proposed by Zhivotovsky (1982) were used. Frequencies of greenbug clones with virulence to sorghum accessions essentially differed. Very high overall and seasonal polymorphism of the insect for virulence was revealed. Among 517 aphid clones tested 33 phenotypes for virulence were identified. The aphid subpopulations collected from the same field at different periods of sorghum vegetation varied significantly in share of rare phenotypes. Criteria of similarities varied from 0.268 to 0.739; according to criterion of identity significant differences between 44 summer subpopulations from 66 studied were found.展开更多
Biogenic isoprene is an important precursor of tropospheric ozone(O_(3)).Here,a coupled chemistry-vegetation model was used to quantify the contributions of isoprene emissions to surface O_(3)pollution on the global s...Biogenic isoprene is an important precursor of tropospheric ozone(O_(3)).Here,a coupled chemistry-vegetation model was used to quantify the contributions of isoprene emissions to surface O_(3)pollution on the global scale during 2000-2019.The biogenic isoprene emissions showed high values in mid-low latitudes and seasonal peaks in the summer hemispheres.They promote global surface O_(3)concentrations by 1.75 ppbv annually with regional hotspots of 4.39 ppbv(8.8%)in China and 5.36 ppbv(11.1%)in the U.S.in boreal summer.In the past two decades,isoprene emissions increased by 1.32 TgC yr^(−1)(0.67%yr^(−1))in the Northern Hemisphere but decreased by 0.71 TgC yr^(−1)(0.44%yr^(−1))in the Southern Hemisphere.Such changes of isoprene made opposite contributions to the surface O_(3)trend,with 0.26 ppbv yr^(−1)in eastern China but−0.32 ppbv yr^(−1)in the southeastern U.S.due to the changes in the background regime of chemical reactions.The impact of anthropogenic changes on the O_(3)trend is consistent with that of biogenic isoprene,but two to four times stronger in magnitude.This study revealed that the effective control of anthropogenic NO_(x)emissions could mitigate regional O_(3)pollution even with the increased isoprene emissions under global warming.展开更多
In recent decades, there have been a number of debates on climate warming and its driving forces. Based on an extensive literature review, we suggest that (1) climate warming occurs with great uncertainty in the mag...In recent decades, there have been a number of debates on climate warming and its driving forces. Based on an extensive literature review, we suggest that (1) climate warming occurs with great uncertainty in the magnitude of the temperature increase; (2) both human activities and natural forces contribute to climate change, but their relative contributions are difficult to quan- tify; and (3) the dominant role of the increase in the atmospheric concentration of greenhouse gases (including CO2) in the global warming claimed by the Intergovernrnental Panel on Climate Change (IPCC) is questioned by the scientific communities because of large uncertainties in the mechanisms of natural factors and anthropogenic activities and in the sources of the increased atmospheric CO2 concentration. More efforts should be made in order to clarify these uncertainties.展开更多
Human disturbance and climate change have increased the risk of extinction for rare and endangered wild plant species.One effective way to conserve these rare and endangered species is through reintroduction.In this r...Human disturbance and climate change have increased the risk of extinction for rare and endangered wild plant species.One effective way to conserve these rare and endangered species is through reintroduction.In this review,we summarize the advances in wild plant reintroduction from five perspectives:the establishment of reintroduction biology as an important tool for biodiversity conservation;the importance of genetic diversity in reintroduction;reintroduction under global climate change;recruitment limitation in reintroduction;and reintroduction and ecological restoration.In addition,we consider the future of plant reintroduction strategies.展开更多
In this paper, we extensively studied a mathematical model of biology. It helps us to understand the dynamical procedure of population changes in biological population model and provides valuable predictions. In this ...In this paper, we extensively studied a mathematical model of biology. It helps us to understand the dynamical procedure of population changes in biological population model and provides valuable predictions. In this model, we establish a variety of exact solutions. To study the exact solutions, we used a fractional complex transform to convert the particular partial differential equation of fractional order into corresponding partial differential equation and modified exp-function method is implemented to investigate the nonlinear equation. Graphical demonstrations along with the numerical data reinforce the efficacy of the used procedure. The specified idea is very effective, unfailing, well-organized and pragmatic for fractional PDEs and could be protracted to further physical happenings.展开更多
This paper investigates the theoretical aspects for an optimal harvesting problem of a nonlinear size-structured population model in a periodic environment. We establish the well-posedness of the state system by means...This paper investigates the theoretical aspects for an optimal harvesting problem of a nonlinear size-structured population model in a periodic environment. We establish the well-posedness of the state system by means of frozen coefficients and fixed point reasoning. The existence of a unique optimal policy is proved via Ekeland's variational principle, and the first-order optimality conditions are derived by a suitable normM cone and a dual system. The results obtained would be beneficial for exploration of renewable展开更多
文摘Chronic hepatitis C is a leading cause of morbidity and mortality, mainly related to fibrosis/cirrhosis and portal hypertension. Direct antiviral agents are highly effective and safe and can now cure > 90% of the patients. Sustained viral response (SVR) after interferon-based regimens has been associated with improvement in liver function, fibrosis and portal hypertension in a significant proportion of patients, although a point of no return seems to exist from which viral elimination is no longer capable of preventing portal hypertension progression and liver decompensation. Indeed, although SVR is associated with improvement of hepatic venous pressure gradients and therefore a decreased risk of de novo esophageal varices, several studies show that viral clearance does not eliminate the risk of variceal progression, liver decompensation and death in patients with pre-established portal hypertension. Although evidence about the effects of direct antiviral agents (DAAs) on clinically significant outcomes is still scarce and with short follow-up, DAAs can decrease the burden of the disease if patients are timely treated before significant fibrosis and portal hypertension develops. Studies with longer follow-up are waited to establish the real magnitude of hepatitis C treatment on portal hypertension. Future studies should also focus on predictors of portal hypertension resolution since it can influence management and avoid unnecessary monitoring
基金National 863 Program(2007AA021107)Beijing Municipal Commission of Education Research Projects(KM201010005008)Doctoral Start-up Foundation of Beijing University of Technology
文摘An optimized recombinant HPV16 E6E7 fusion gene (HPV16 ofE6E7) was constructed according to codon usage for mammalian cell expression, and a mutant of HPV16 ofE6E7 fusion gene (HPV16 omfE6E7) was generated by site-directed mutagenesis at L57G, C113R for the E6 protein and C24G, E26G for the E7 protein for HPV16 ofE6E7 [patent pending (CN 101100672)]. The HPV16 omfE6E7 gene constructed in this work not only lost the transformation capability to NIH 3T3 cells and tumorigenicity in SCID mice, but also maintained very good stability and antigenicity. These results suggests that the HPV16 omfE6E7 gene should undergo further study for application as a safe antigen-specific therapeutic vaccine for HPV16-associated tumors.
基金Under the auspices of Strategic and Special Frontier Project of Science and Technology of Chinese Academy of Sciences (No. XDA05080800)National Basic Research Program of China (No. 2010CB950102)National Natural Science Foundation of China (No. 40871007)
文摘Three global datasets, the History Database of the Global Environment (HYDE), Kaplan and Krurnhardt (KK) and Pongratz of reconstructed anthropogenic land cover change (ALCC) were introduced and compared in this paper. The HYDE dataset was recon- structed by Goldewijk and his colleagues at the National institute of Public ttealth and the Environment in Netherland, covering the past 12 000 years. The KK dataset was reconstructed by Kaplan and his colleagues, the Soil-Vegetation-Atmosphere Research Group at the Institute of Environmental Engineering in Switzerland, covering the past 8000 years. The Pongratz dataset was reconstructed by Pon- gratz and her colleagues at the Max Planck Institute for Meteorology in Germany, coveting AD 800-1992. The results show that the reconstructed datasets are quite different from each other due to the different methods used. The three datasets all allocated the historical ALCC according to human population density. The main reason causing the differences among the three datasets lies on the different relationships between population density and land use used in each reconstructed dataset. The KK dataset is better than the other two datasets for two important reasons. First, it used the nonlinear relationship between population density and land use, while the other two used the linear relationship. Second, Kaplan and his colleagues adopted the technological development and intensification parameters and considered the wood harvesting and the long-term fallow area resulted from shifting cultivation, which were neglected in the recon- structions of the other two datasets. Therefore, the KK dataset is more suitable as one of the anthropogenic forcing fields for climate simulation over the past two millennia that is recently concerned by two projects, the National Basic Research Program and the Strategic and Special Frontier Project of Science and Technology of the Chinese Academy of Sciences.
文摘Intraspecific variability in Schizaphis graminum (Rondani) was studied in 2002-2005. Aphid clones were sampled from sorghum field at the Kuban Experimental Station (Krasnodar region, Northern Caucasus, Russia) in June (active greenbug migration to the field), July (high rate of the population increasing), and August (greenbug abundance decreasing) and at the end of September or in the beginning of October (appearance of sexual males and females). Damage rating was estimated for two plant sets composed of three sorghum differentials. Set A contains Deer (gene for resistance Sgr4), Sarvasi (Sgrl + Sgr2) and Capbam (Sgrl2). Set B contains Shallu (Sgr3), Sorgogradskoe (Sgr5) and Durra Belaya (Sgr5 + Sgr6). To estimate variability in greenbug subpopulations criteria proposed by Zhivotovsky (1982) were used. Frequencies of greenbug clones with virulence to sorghum accessions essentially differed. Very high overall and seasonal polymorphism of the insect for virulence was revealed. Among 517 aphid clones tested 33 phenotypes for virulence were identified. The aphid subpopulations collected from the same field at different periods of sorghum vegetation varied significantly in share of rare phenotypes. Criteria of similarities varied from 0.268 to 0.739; according to criterion of identity significant differences between 44 summer subpopulations from 66 studied were found.
基金supported by the National Key Research and Development Program of China[grant number 2023YFF0805403]the National Natural Science Foundation of China[grant number 41975155].
文摘Biogenic isoprene is an important precursor of tropospheric ozone(O_(3)).Here,a coupled chemistry-vegetation model was used to quantify the contributions of isoprene emissions to surface O_(3)pollution on the global scale during 2000-2019.The biogenic isoprene emissions showed high values in mid-low latitudes and seasonal peaks in the summer hemispheres.They promote global surface O_(3)concentrations by 1.75 ppbv annually with regional hotspots of 4.39 ppbv(8.8%)in China and 5.36 ppbv(11.1%)in the U.S.in boreal summer.In the past two decades,isoprene emissions increased by 1.32 TgC yr^(−1)(0.67%yr^(−1))in the Northern Hemisphere but decreased by 0.71 TgC yr^(−1)(0.44%yr^(−1))in the Southern Hemisphere.Such changes of isoprene made opposite contributions to the surface O_(3)trend,with 0.26 ppbv yr^(−1)in eastern China but−0.32 ppbv yr^(−1)in the southeastern U.S.due to the changes in the background regime of chemical reactions.The impact of anthropogenic changes on the O_(3)trend is consistent with that of biogenic isoprene,but two to four times stronger in magnitude.This study revealed that the effective control of anthropogenic NO_(x)emissions could mitigate regional O_(3)pollution even with the increased isoprene emissions under global warming.
基金supported by the Academic Division of the Chinese Academy of Sciencesthe National Natural Science Foundation of China (Grant No. 31021001)the National Basic Research Program of China (Grant No. 2010CB950600)
文摘In recent decades, there have been a number of debates on climate warming and its driving forces. Based on an extensive literature review, we suggest that (1) climate warming occurs with great uncertainty in the magnitude of the temperature increase; (2) both human activities and natural forces contribute to climate change, but their relative contributions are difficult to quan- tify; and (3) the dominant role of the increase in the atmospheric concentration of greenhouse gases (including CO2) in the global warming claimed by the Intergovernrnental Panel on Climate Change (IPCC) is questioned by the scientific communities because of large uncertainties in the mechanisms of natural factors and anthropogenic activities and in the sources of the increased atmospheric CO2 concentration. More efforts should be made in order to clarify these uncertainties.
基金supported by the National Key Fundamental Research Development Plan (2009CB421101)the National Natural Science Foundation of China (40871249,30670370)
文摘Human disturbance and climate change have increased the risk of extinction for rare and endangered wild plant species.One effective way to conserve these rare and endangered species is through reintroduction.In this review,we summarize the advances in wild plant reintroduction from five perspectives:the establishment of reintroduction biology as an important tool for biodiversity conservation;the importance of genetic diversity in reintroduction;reintroduction under global climate change;recruitment limitation in reintroduction;and reintroduction and ecological restoration.In addition,we consider the future of plant reintroduction strategies.
文摘In this paper, we extensively studied a mathematical model of biology. It helps us to understand the dynamical procedure of population changes in biological population model and provides valuable predictions. In this model, we establish a variety of exact solutions. To study the exact solutions, we used a fractional complex transform to convert the particular partial differential equation of fractional order into corresponding partial differential equation and modified exp-function method is implemented to investigate the nonlinear equation. Graphical demonstrations along with the numerical data reinforce the efficacy of the used procedure. The specified idea is very effective, unfailing, well-organized and pragmatic for fractional PDEs and could be protracted to further physical happenings.
文摘This paper investigates the theoretical aspects for an optimal harvesting problem of a nonlinear size-structured population model in a periodic environment. We establish the well-posedness of the state system by means of frozen coefficients and fixed point reasoning. The existence of a unique optimal policy is proved via Ekeland's variational principle, and the first-order optimality conditions are derived by a suitable normM cone and a dual system. The results obtained would be beneficial for exploration of renewable