AIM： To investigate the characteristics of slow electrical waves and the presence of transient receptor potential melastatin-type 7 （TRPM7） in the human gastrointestinal （GI） tract. METHODS： Conventional microel...AIM： To investigate the characteristics of slow electrical waves and the presence of transient receptor potential melastatin-type 7 （TRPM7） in the human gastrointestinal （GI） tract. METHODS： Conventional microelectrode techniques were used to record intracellular electrical responses from human GI smooth muscle tissue. Immunohistochemistry was used to identify TRPM7 channels in interstitial cells of Cajat （ICCs）. RESULTS： The human GI tract generated slow electrical waves and had ICCs which functioned as pacemak er cells. Flufenamic acid, a nonselective cation channel blocker, and 2-APB （2-aminoethoxydiphenyl borate） and La3＋, TRPM7 channel blockers, inhibited the slowwaves. Also, TRPM7 channels were expressed in ICCs in human tissue. CONCLUSION： These results suggest that the human GI tract generates slow waves and that TRPM7 channels expressed in the ICCs may be involved in the gen- eration of the slow waves.展开更多
The effect of monoethylphosphate (MEP, commercial available or synthesized) together with IL-2 on the selective proliferation of human γ~δ T cells in Vitro from peripheral blood mononuclear cells (PBMC) of healthy d...The effect of monoethylphosphate (MEP, commercial available or synthesized) together with IL-2 on the selective proliferation of human γ~δ T cells in Vitro from peripheral blood mononuclear cells (PBMC) of healthy donors and of cancer patients was investigated. The γ~δ T cells were stimulated by MEP to proliferate in a dose-dependent manner. The effect of synthesized MEP was 10 times greater than that of commercial MEP. When the PBMCs of healthy donors were cultured for 25 d in the medium containing different concentrations of MEP, the total cell number increased about 1000-3000 fold; and the ratio of γ~δ T cells reached to 70-80%. The selective expansion of γ~δ T cells depended on the synergic action of MEP and IL-2. The bulk cultured γ~δ T cells exhibited obvious cytotoxic activities against allogenic tumor cell lines (SQ-5,K562 alld Daudi) and autologous tumor cells. The culture system described here not only offers a simple method for obtaining a large number of γ~δ T cells which may become a new effector in the adoptive immunotherapy, but also provides a useful model for the further studies of the structure and function of γ~δ T cells in vitro.展开更多
The ongoing research for model choice and selection has generated a plethora of approaches. With such a wealth of methods, it can be difficult for a researcher to know what model selection approach is the proper w...The ongoing research for model choice and selection has generated a plethora of approaches. With such a wealth of methods, it can be difficult for a researcher to know what model selection approach is the proper way to proceed to select the appropriate model for prediction. The authors present an evaluation of various model selection criteria from decision-theoretic perspective using experimental data to define and recommend a criterion to select the best model. In this analysis, six of the most common selection criteria, nineteen friction factor correlations, and eight sets of experimental data are employed. The results show that while the use of the traditional correlation coefficient, R2 is inappropriate, root mean square error, RMSE can be used to rank models, but does not give much insight on their accuracy. Other criteria such as correlation ratio, mean absolute error, and standard deviation are also evaluated. The AIC （Akaike Information Criterion） has shown its superiority to other selection criteria. The authors propose AIC as an alternative to use when fitting experimental data or evaluating existing correlations. Indeed, the AIC method is an information theory based, theoretically sound and stable. The paper presents a detailed discussion of the model selection criteria, their pros and cons, and how they can be utilized to allow proper comparison of different models for the best model to be inferred based on sound mathematical theory. In conclusion, model selection is an interesting problem and an innovative strategy to help alleviate similar challenges faced by the professionals in the oil and gas industry is introduced.展开更多
基金Supported by The Creative Research Initiative Center for Bio-Artificial Muscle of the Ministry of Education,Science and Technology (MEST) in Korea
文摘AIM： To investigate the characteristics of slow electrical waves and the presence of transient receptor potential melastatin-type 7 （TRPM7） in the human gastrointestinal （GI） tract. METHODS： Conventional microelectrode techniques were used to record intracellular electrical responses from human GI smooth muscle tissue. Immunohistochemistry was used to identify TRPM7 channels in interstitial cells of Cajat （ICCs）. RESULTS： The human GI tract generated slow electrical waves and had ICCs which functioned as pacemak er cells. Flufenamic acid, a nonselective cation channel blocker, and 2-APB （2-aminoethoxydiphenyl borate） and La3＋, TRPM7 channel blockers, inhibited the slowwaves. Also, TRPM7 channels were expressed in ICCs in human tissue. CONCLUSION： These results suggest that the human GI tract generates slow waves and that TRPM7 channels expressed in the ICCs may be involved in the gen- eration of the slow waves.
文摘The effect of monoethylphosphate (MEP, commercial available or synthesized) together with IL-2 on the selective proliferation of human γ~δ T cells in Vitro from peripheral blood mononuclear cells (PBMC) of healthy donors and of cancer patients was investigated. The γ~δ T cells were stimulated by MEP to proliferate in a dose-dependent manner. The effect of synthesized MEP was 10 times greater than that of commercial MEP. When the PBMCs of healthy donors were cultured for 25 d in the medium containing different concentrations of MEP, the total cell number increased about 1000-3000 fold; and the ratio of γ~δ T cells reached to 70-80%. The selective expansion of γ~δ T cells depended on the synergic action of MEP and IL-2. The bulk cultured γ~δ T cells exhibited obvious cytotoxic activities against allogenic tumor cell lines (SQ-5,K562 alld Daudi) and autologous tumor cells. The culture system described here not only offers a simple method for obtaining a large number of γ~δ T cells which may become a new effector in the adoptive immunotherapy, but also provides a useful model for the further studies of the structure and function of γ~δ T cells in vitro.
文摘The ongoing research for model choice and selection has generated a plethora of approaches. With such a wealth of methods, it can be difficult for a researcher to know what model selection approach is the proper way to proceed to select the appropriate model for prediction. The authors present an evaluation of various model selection criteria from decision-theoretic perspective using experimental data to define and recommend a criterion to select the best model. In this analysis, six of the most common selection criteria, nineteen friction factor correlations, and eight sets of experimental data are employed. The results show that while the use of the traditional correlation coefficient, R2 is inappropriate, root mean square error, RMSE can be used to rank models, but does not give much insight on their accuracy. Other criteria such as correlation ratio, mean absolute error, and standard deviation are also evaluated. The AIC （Akaike Information Criterion） has shown its superiority to other selection criteria. The authors propose AIC as an alternative to use when fitting experimental data or evaluating existing correlations. Indeed, the AIC method is an information theory based, theoretically sound and stable. The paper presents a detailed discussion of the model selection criteria, their pros and cons, and how they can be utilized to allow proper comparison of different models for the best model to be inferred based on sound mathematical theory. In conclusion, model selection is an interesting problem and an innovative strategy to help alleviate similar challenges faced by the professionals in the oil and gas industry is introduced.
