Despite significant advances in monoclonal antibodies (Mabs) technology over the last two decades, technology to develop natural occurring and fully human therapeutic Mabs from patients without known antigens is still...Despite significant advances in monoclonal antibodies (Mabs) technology over the last two decades, technology to develop natural occurring and fully human therapeutic Mabs from patients without known antigens is still in its infancy. Human myeloma cell lines have been very difficult to derive. Attempts in numerous laboratories to use those cells for the production of human monoclonal antibodies have failed despite early reports. A human myeloma cell line that is suitable for the generation of human monoclonal antibodies (Proceedings of the National Academy of Sciences USA 98:1799-1804) was developed by Dr. Karpas. Since 1975 researchers across the world have been trying to replicate the process with human cells.展开更多
OBJECTIVE: To establish a method for rapid detection and sub-typing of human papilloma virus (HPV). METHODS: We utilized the piezoelectric genosensor (PG) technique, which is a combination of the piezoelectric biosens...OBJECTIVE: To establish a method for rapid detection and sub-typing of human papilloma virus (HPV). METHODS: We utilized the piezoelectric genosensor (PG) technique, which is a combination of the piezoelectric biosensor and gene chips for HPV identification in 22 recurrent biopsy specimens and 22 corresponding original biopsy specimens. The control samples came from normal tissue of healthy persons. A combined reaction took place on the sensor surface between the target genes and probes. The frequency of the piezoelectric sensor will decrease when such reactions occur, and the frequency decrease depends on the concentration of the target gene. Specimens were also analyzed with conventional PCR and dot blot. RESULTS: Of the 22 recurrent specimens, 15 contained HPV6 DNA, 2 HPV11 DNA, and 4 HPV16 DNA. Only one specimen was negative. All the 22 original specimens were positive: 17 harbored HPV6 DNA, 3 sequence homologous HPV11 DNA, and 2 HPV16 DNA. No HPV18 DNA was detected in any specimen. When compared with PCR and dot blot analysis, the results were essentially the same except for one specimen, which was shown to contain other sub-types of HPV. CONCLUSION: Our results show that the piezoelectric genosensor technique is a rapid and specific method to analyze HPV.展开更多
文摘Despite significant advances in monoclonal antibodies (Mabs) technology over the last two decades, technology to develop natural occurring and fully human therapeutic Mabs from patients without known antigens is still in its infancy. Human myeloma cell lines have been very difficult to derive. Attempts in numerous laboratories to use those cells for the production of human monoclonal antibodies have failed despite early reports. A human myeloma cell line that is suitable for the generation of human monoclonal antibodies (Proceedings of the National Academy of Sciences USA 98:1799-1804) was developed by Dr. Karpas. Since 1975 researchers across the world have been trying to replicate the process with human cells.
基金supported by the National Natural Scienee Foundation of China(No．39870831)；the High-Tech Research and Development (863)Programme and the Key Technologies R&D Programme of the Nineth Five-vear Plan for the National Scientifie and Technological Development(No．96-A23-04-04)．
文摘OBJECTIVE: To establish a method for rapid detection and sub-typing of human papilloma virus (HPV). METHODS: We utilized the piezoelectric genosensor (PG) technique, which is a combination of the piezoelectric biosensor and gene chips for HPV identification in 22 recurrent biopsy specimens and 22 corresponding original biopsy specimens. The control samples came from normal tissue of healthy persons. A combined reaction took place on the sensor surface between the target genes and probes. The frequency of the piezoelectric sensor will decrease when such reactions occur, and the frequency decrease depends on the concentration of the target gene. Specimens were also analyzed with conventional PCR and dot blot. RESULTS: Of the 22 recurrent specimens, 15 contained HPV6 DNA, 2 HPV11 DNA, and 4 HPV16 DNA. Only one specimen was negative. All the 22 original specimens were positive: 17 harbored HPV6 DNA, 3 sequence homologous HPV11 DNA, and 2 HPV16 DNA. No HPV18 DNA was detected in any specimen. When compared with PCR and dot blot analysis, the results were essentially the same except for one specimen, which was shown to contain other sub-types of HPV. CONCLUSION: Our results show that the piezoelectric genosensor technique is a rapid and specific method to analyze HPV.
