A cDNA fragment encoding human cathelicidin LL- 37 linked to a signal peptide sequence of tobacco pathogenesis-related protein lb gene was introduced into a plant expression vector, pBin438 and stably integrated into ...A cDNA fragment encoding human cathelicidin LL- 37 linked to a signal peptide sequence of tobacco pathogenesis-related protein lb gene was introduced into a plant expression vector, pBin438 and stably integrated into Nicotiana tabacum cv. NC - 89 genome using Agrobacterium mmefaciens - mediated transformation. The presence of the fusion cDNA in the transgenic plant was confirmed by PCR and its expression was confirmed by Western blot analysis. Protein extracts from transgenic tobacco plant leaves exhibited antibacterial activity. This is the first report of synthesis of biologically active LL - 37 in plants.展开更多
文摘A cDNA fragment encoding human cathelicidin LL- 37 linked to a signal peptide sequence of tobacco pathogenesis-related protein lb gene was introduced into a plant expression vector, pBin438 and stably integrated into Nicotiana tabacum cv. NC - 89 genome using Agrobacterium mmefaciens - mediated transformation. The presence of the fusion cDNA in the transgenic plant was confirmed by PCR and its expression was confirmed by Western blot analysis. Protein extracts from transgenic tobacco plant leaves exhibited antibacterial activity. This is the first report of synthesis of biologically active LL - 37 in plants.
