Presently the gold standard diagnostic technique for rabies is the direct immunofluorescence assay （dFA） which is very expensive and requires a high level of expertise. There is a need for more economical and user f...Presently the gold standard diagnostic technique for rabies is the direct immunofluorescence assay （dFA） which is very expensive and requires a high level of expertise. There is a need for more economical and user friendly tests, particularly for use in developing countries. We have established one such test called the direct rapid immunohistochemical test （dRIT） for diagnosis of rabies using brain tissue. The test is based on capture of rabies nucleoprotein （N） antigen in brain smears using a cocktail of biotinylated monoclonal antibodies specific for the N protein and color development by streptavidin peroxidase-amino ethyl carbazole and counter staining with haematoxollin. The test was done in parallel with standard FAT dFA using 400 brain samples from different animals and humans. The rabies virus N protein appears under fight microscope as reddish brown particles against a light blue background. There was 100 % correlation between the results obtained by the two tests. Also, interpretation of results by dRIT was easier and only required a light microscope. To conclude, this newly developed dRIT technique promises to be a simple, cost effective diagnostic tool for rabies and will have applicability in field conditions prevalent in developing countries.展开更多
The Pursuit of Happyness has always been a well-received film ever since its release in 2006. This film mainly describes how a black American worms his way from the bottom of the society to success. This paper intends...The Pursuit of Happyness has always been a well-received film ever since its release in 2006. This film mainly describes how a black American worms his way from the bottom of the society to success. This paper intends to make a detailed description of the life experience of the main character, including his life obstacles and fighting. It also expounds on the image of the main character. Moreover, through the analysis of the reasons why Chris, an average American with his unyielding spirits, succeeds in seizing his happiness through sorrow and pain in life, the practical significance with regard to individual and social development is put into the limelight.展开更多
Exponential increase of anthropogenic impact （human population number, some technological parameters） becomes menacing for biosphere functioning. Anyway, we should be able to estimate quantitatively limits of our im...Exponential increase of anthropogenic impact （human population number, some technological parameters） becomes menacing for biosphere functioning. Anyway, we should be able to estimate quantitatively limits of our impact on functional parameters of the biosphere. Considering biosphere as a natural life-support system （LSS）, we can receive the helpful information for working out and creation of artificial LSS of various types. Big biotic cycle induced with flows of a solar energy, is a basis of functioning of the biosphere and its basic cells-ecosystems. It＇s possible to summarize briefly the main functional and structural properties of the biosphere： integrity, closure, substance cycling, steady state, energy dependence and biodiversity. These properties of the biosphere, as a LSS, ensure potentially everlasting life under the conditions of a limited quantity of substrate suitable for the life on the planet. Ecological Footprint （EF） as a quantitative measure of anthropogenic impact on biosphere functioning is discussed in the paper. The index of the ecological reliability （IER） is introduced as a quantitative ecological indicator of different territories. The comparative dynamics of the United Nations＇ Human Development Index （HDI） and EF is discussed. The vital goal of sustainable human development： all humans can have opportunity to fulfill their lives without degrading the biosphere. To support sustainability, we should try to develop each nation and the mankind as a whole with a high HDI and a low ecological footprint. It means to have high level of HDI at low level of EF. But current tendency of economical and social development shows that the higher HDI is, the bigger EF is. EF of mankind is growing menacingly. Now actual pressure of the human civilization of our planet （2010） upon 50% exceeds its potential possibilities biological capacity （BC）, measured on the area ＂global＂ green hectares）. It means that we need 1.5 planets of the Earth＇s type. It leads to ecological incident in the scale of biosphere. Our biosphere is the large, multilevel, hierarchically organized system, and our civilization is only a part of it. This part is not central; it can disappear for ever, if we do not cope to be included in the biosphere as a great system.展开更多
In the past few years China has consistently and increasingly encouraged innovation,making great headway in such sectors as heavy equipment manufacturing,high-speed rail,biopharmaceuticals,new energy and robotics.Inno...In the past few years China has consistently and increasingly encouraged innovation,making great headway in such sectors as heavy equipment manufacturing,high-speed rail,biopharmaceuticals,new energy and robotics.Innovation has become an important engine boosting economic and social development in China.展开更多
Underground building with such major functions as underground traffic, commerce, culture and recreation, especially the multifunctional underground large-scale public complex, has become a significant part of the over...Underground building with such major functions as underground traffic, commerce, culture and recreation, especially the multifunctional underground large-scale public complex, has become a significant part of the overaU functional structure or even the core of a city. Therefore, the expression of the architectural appearance, and characters of underground building, as well as the integration between the building and its external environment constitute an important part in improving overall functional structure and optimizing cityscape. From the perspective of space and environment shaping, the author explores the architectural appearance, and characters of underground building and its external environment, provides several basic design patterns with further discussion.展开更多
文摘Presently the gold standard diagnostic technique for rabies is the direct immunofluorescence assay （dFA） which is very expensive and requires a high level of expertise. There is a need for more economical and user friendly tests, particularly for use in developing countries. We have established one such test called the direct rapid immunohistochemical test （dRIT） for diagnosis of rabies using brain tissue. The test is based on capture of rabies nucleoprotein （N） antigen in brain smears using a cocktail of biotinylated monoclonal antibodies specific for the N protein and color development by streptavidin peroxidase-amino ethyl carbazole and counter staining with haematoxollin. The test was done in parallel with standard FAT dFA using 400 brain samples from different animals and humans. The rabies virus N protein appears under fight microscope as reddish brown particles against a light blue background. There was 100 % correlation between the results obtained by the two tests. Also, interpretation of results by dRIT was easier and only required a light microscope. To conclude, this newly developed dRIT technique promises to be a simple, cost effective diagnostic tool for rabies and will have applicability in field conditions prevalent in developing countries.
文摘The Pursuit of Happyness has always been a well-received film ever since its release in 2006. This film mainly describes how a black American worms his way from the bottom of the society to success. This paper intends to make a detailed description of the life experience of the main character, including his life obstacles and fighting. It also expounds on the image of the main character. Moreover, through the analysis of the reasons why Chris, an average American with his unyielding spirits, succeeds in seizing his happiness through sorrow and pain in life, the practical significance with regard to individual and social development is put into the limelight.
文摘Exponential increase of anthropogenic impact （human population number, some technological parameters） becomes menacing for biosphere functioning. Anyway, we should be able to estimate quantitatively limits of our impact on functional parameters of the biosphere. Considering biosphere as a natural life-support system （LSS）, we can receive the helpful information for working out and creation of artificial LSS of various types. Big biotic cycle induced with flows of a solar energy, is a basis of functioning of the biosphere and its basic cells-ecosystems. It＇s possible to summarize briefly the main functional and structural properties of the biosphere： integrity, closure, substance cycling, steady state, energy dependence and biodiversity. These properties of the biosphere, as a LSS, ensure potentially everlasting life under the conditions of a limited quantity of substrate suitable for the life on the planet. Ecological Footprint （EF） as a quantitative measure of anthropogenic impact on biosphere functioning is discussed in the paper. The index of the ecological reliability （IER） is introduced as a quantitative ecological indicator of different territories. The comparative dynamics of the United Nations＇ Human Development Index （HDI） and EF is discussed. The vital goal of sustainable human development： all humans can have opportunity to fulfill their lives without degrading the biosphere. To support sustainability, we should try to develop each nation and the mankind as a whole with a high HDI and a low ecological footprint. It means to have high level of HDI at low level of EF. But current tendency of economical and social development shows that the higher HDI is, the bigger EF is. EF of mankind is growing menacingly. Now actual pressure of the human civilization of our planet （2010） upon 50% exceeds its potential possibilities biological capacity （BC）, measured on the area ＂global＂ green hectares）. It means that we need 1.5 planets of the Earth＇s type. It leads to ecological incident in the scale of biosphere. Our biosphere is the large, multilevel, hierarchically organized system, and our civilization is only a part of it. This part is not central; it can disappear for ever, if we do not cope to be included in the biosphere as a great system.
文摘In the past few years China has consistently and increasingly encouraged innovation,making great headway in such sectors as heavy equipment manufacturing,high-speed rail,biopharmaceuticals,new energy and robotics.Innovation has become an important engine boosting economic and social development in China.
文摘Underground building with such major functions as underground traffic, commerce, culture and recreation, especially the multifunctional underground large-scale public complex, has become a significant part of the overaU functional structure or even the core of a city. Therefore, the expression of the architectural appearance, and characters of underground building, as well as the integration between the building and its external environment constitute an important part in improving overall functional structure and optimizing cityscape. From the perspective of space and environment shaping, the author explores the architectural appearance, and characters of underground building and its external environment, provides several basic design patterns with further discussion.
