人类外周血hPer1基因节律性表达的研究

目的生物节律广泛存在于人类生活和工作中,为研究正常人节律基因表达特点,定量检测正常年轻人外周血中节律基因Per1(humanPeriod1,hPer1)的表达。方法实验对象为7名大学生(H1-H7),年龄24￣30岁,男性3名,女性4名。其中H7于实验前1天晚上值夜班。分别在09:00,15:00,21:00,03:00,09:00点抽取肘静脉血6mL,抽提全血总RNA。应用竞争性RT-PCR方法定量检测实验对象外周血中每人每个时间点hPer1基因的表达。结果人类外周血中hPer1基因的表达有显著节律性。表达高峰在09:00而低峰在21:00,两者之间的差异达2.29±0.9倍(P<0.01),第2天09:00hPer1基因表达水平又回到最高峰。结论hPer1基因的表达在外周血中存在节律性。

人外周血钟基因表达研究进展

生物钟基因普遍存在于生物界，包括人类。其作用在于产生和控制昼夜节律的运转。除下丘脑视交叉上核中枢钟外，人类外周血细胞中也存在各种生物钟成分，同样以转录翻译反馈环路的形式与下丘脑视交叉上核构成一套完整的振荡系统与环境周期保持同步。但其钟基因表达和调控有其自身特点。该文主要介绍了人类外周血中钟基因的组成和生理及病理情况下其调控机制以及研究意义及展望。

血管内皮祖细胞功能的影响因素

1997年Asahara等[1]第一次发现从人类外周血中纯化出的CD34＋/Flk-1＋细胞,在体外培养过程中能分化成为具有内皮表型的细胞,并在后肢缺血模型裸鼠体内静脉注入荧光染色的CD34＋单核细胞,发现其对血管新生有促进作用,自此血管内皮祖细胞（endothelial progenitor cells,EPCs）走进人们视线,它因与血管形成、血管再生及修复息息相关而备受瞩目,并且开启了人类对其长达十余年的研究历程,

外周血HLA-DR^(+)/CD14+水平对重症急性胰腺炎预后评估的价值

目的探讨外周血CD14^(+)单核细胞人类白细胞DR抗原(HLA-DR^(+)/CD14^(+))水平对重症急性胰腺炎(SAP)预后评估的价值。方法2016年2月至2018年2月该院收治的97例SAP患者根据预后结局分为预后良好组(46例)和预后不良组(51例)。常规收集血钙、氧合指数(PaO2/FiO2)、尿素氮、肌酐、急性生理与慢性健康(APACHEⅡ)评分、急性胰腺炎严重程度床边指数(BISAP)评分、兰森(Ranson)评分。流式细胞术检测外周血HLA-DR^(+)/CD14^(+)水平并进行相关性分析,logistic回归分析不良预后发生危险因素。结果预后不良组尿素氮水平、肌酐水平、APACHEⅡ评分、BISAP评分、Ranson评分均明显高于预后良好组(P<0.05),血钙、PaO2/FiO2、HLA-DR^(+)/CD14^(+)水平明显低于预后良好组(P<0.05)。外周血HLA-DR^(+)/CD14^(+)水平与肌酐水平、APACHEⅡ评分、BISAP评分、Ranson评分均呈负相关(P<0.05)。高BISAP评分、高APACHEⅡ评分、低HLA-DR^(+)/CD14^(+)水平是影响SAP患者不良预后发生危险因素。外周血HLA-DR^(+)/CD14^(+)水平预测SAP不良预后发生的曲线下面积为0.896,灵敏度为84.30%,特异度为84.80%。结论不良预后SAP患者外周血HLA-DR^(+)/CD14^(+)水平明显降低,可能作为预后评估指标。

Decreased mitochondrial deoxyribonucleic acid and increased oxidative damage in chronic hepatitis C

AIM： To determine whether alteration of the mito- chondria DNA （mtDNA） copy number and its oxidative damage index （mtDNA△CT） can be detected by analysis of peripheral blood cells in hepatitis C virus （HCV）- infected patients. METHODS： This study enrolled two groups of pa- tients aged 40-60 years： a control group and an HCV- infected group in Department of Gastroenterology and Hepatology in Changhua Christian Hospital. Patients with co-infection with hepatitis B virus or human im- munodeficiency virus, autoimmune disease, malignant neoplasia, pregnancy, thyroid disease, or alcohol con- sumption 〉 40 g/d were excluded. HCV-infected pa- tients who met the following criteria were included： （1） positive HCV antibodies for 〉 6 mo; （2） alanine aminotransferase （ALT） levels more than twice the upper lim- it of normal on at least two occasions during the past 6 mo; and （3） histological fibrosis stage higher than F1. The mtDNA copy number and oxidative damage index of HCV mtDNA （mtDNA△CT） were measured in periph- eral blood leukocytes. The association between mtDNA copy number and mtDNA△CT was further analyzed using clinical data. RESULTS： Forty-seven normal controls （male/female： 26/21, mean age 50.51 ± 6.15 years） and 132 HCV- infected patients （male/female： 76/61, mean age 51.65 ± 5.50 years） were included in the study. The geno- types of HCV-infected patients include type 1a （n = 3）, type 1b （n = 83）, type 2a （n = 32）, and type 2b （n = 14）. Liver fibrosis stages were distributed as follows： F1/F2/F3/F4 = 1/61/45/25 and activity scores were A0/ A1/A2/A3 = 7/45/55/25. There were no age or gender differences between the two groups. HCV-infected pa- tients had higher hepatitis activity （aspartate transami- nase levels 108.77 ± 60.73 vs 23.19 ± 5.47, P 〈 0.01; ALT levels 168.69 ± 93.12 vs 23.15 ± 9.45, P 〈 0.01） and lower platelet count （170.40±58.00 vs 251.24 ± 63.42, P 〈 0.01） than controls. The mtDNA copy num- ber was lower in HCV-infected patients than in controls （173.49 vs 247.93, P 〈 0.05）. The mtDNA△CT was higher in HCV-infected patients than in controls （2.92 vs 0.64, P 〈 0.05）. To clarify the clinical significance of these results in HCV-infected patients, their association with different clinical parameters among HCV-infected pa- tients was analyzed. A negative association was found between mtDNA copy number and elevated aspartate transaminase levels （r = -0.17, P 〈 0.05）. Changes in mtDNA copy number were not associated with HCV RNA levels, HCV genotypes, liver fibrosis severity, or inflammatory activity in the liver biopsy specimen. How- ever, a correlation was observed between mtDNA△CT and platelet count （r = -0.22, P 〈 0.01）, HCV RNA level （r = 0.36, P 〈 0.01）, and hepatitis activity （r = 0.20, P = 0.02）. However, no difference in the change in mtDNA△CT was observed between different fibrosis stages or HCV CONCLUSION： Oxidative stress and mtDNA dam- age are detectable in patient＇s peripheral leukocytes. Increased leukocyte mtDNA△CT correlates with higher HCV viremia, increased hepatitis activity, and lower platelet count.

肺炎患者PCT和单核细胞DR分子表达的研究

目的:研究细菌性肺炎患者血清降钙素原(PCT)和外周血单核细胞人类白细胞DR抗原(HLA-DR/CD14+)表达水平的关系。方法:检测30例细菌性肺炎组和30健康对照组的PCT、CRP和外周血单核细胞人类白细胞DR抗原(HLA-DR/CD14+)表达水平,比较组间的差异,分析PCT、CRP与HLA-DR/CD14+的关系。结果:细菌性肺炎组患者外周血细胞HLA-DR/CD14+细胞百分率显著低于正常对照组(P<0.05),血清PCT和CRP水平显著高于正常组(P<0.05)。外周血细胞HLA-DR/CD14+细胞百分率与血清PCT结果存在高度负相关(r=-0.318,P<0.05),而外周血细胞HLA-DR/CD14+细胞百分率与血清CRP水平无相关性(r=-0.279,P>0.05)。结论:细菌性肺炎患者血清PCT和外周血HLA-DR/CD14+表达水平存在异常,外周血HLA-DR/CD14+作为细胞水平上的指标对肺炎诊断具有一定得临床指导意义。