目的探讨应用非诺贝特联合热量限制饮食治疗非酒精性脂肪性肝病(NAFLD)合并2型糖尿病(T2DM)患者的疗效及患者血清人类软骨糖蛋白-39(HCGP39)、脂联素(APN)和非对称二甲基精氨酸(ADMA)水平的变化。方法将128例NAFLD合并T2DM患者随机分为...目的探讨应用非诺贝特联合热量限制饮食治疗非酒精性脂肪性肝病(NAFLD)合并2型糖尿病(T2DM)患者的疗效及患者血清人类软骨糖蛋白-39(HCGP39)、脂联素(APN)和非对称二甲基精氨酸(ADMA)水平的变化。方法将128例NAFLD合并T2DM患者随机分为对照组64例和观察组64例,分别采用热量限制饮食或在此基础上给予非诺贝特口服治疗、观察12周。采用免疫层析法检测血清糖化血红蛋白(HbA1c)水平,采用葡萄糖氧化酶法检测血清空腹血糖(FPG)和餐后2h血糖(2 h PPG)水平,采用磁微粒化学发光法检测血清空腹胰岛素(FINS)水平,并计算胰岛素抵抗(IR)指数。采用ELISA法检测血清HCGP39、APN和ADMA水平。结果在治疗结束时,观察组血清谷丙转氨酶(ALT)水平为(39.4±5.3)U/L,显著低于对照组【(46.8±7.9)U/L,P<0.05】,血清谷草转氨酶(AST)水平为(46.3±4.4)U/L,显著低于对照组【(54.7±7.5)U/L,P<0.05】,血清谷氨酰转移酶(GGT)水平为(33.9±8.6)U/L,显著低于对照组【(43.7±14.6)U/L,P<0.05】;血清FBG水平为(5.9±1.9)mmol/L,显著低于对照组【(6.8±2.0)mmol/L,P<0.05】,血清2 h PPG水平为(7.4±2.2)mmol/L,显著低于对照组【(8.5±2.4)mmol/L,P<0.05】,IR水平为(4.1±0.4),显著低于对照组【(4.7±0.5),P<0.05】;血清总胆固醇(TC)水平为(1.5±0.2)mmol/L,显著低于对照组【(2.1±0.2)mmol/L,P<0.05】,血清三酰甘油(TG)水平为(4.0±0.7)mmol/L,显著低于对照组【(5.5±0.8)mmol/L,P<0.05】,血清低密度脂蛋白(LDL)水平为(2.1±0.5)mmol/L,显著低于对照组【(2.5±0.6)mmol/L,P<0.05】,而血清高密度脂蛋白(HDL)水平为(1.6±0.5)mmol/L,显著高于对照组【(1.4±0.3)mmol/L,P<0.05】;血清HCGP39水平为(61.4±7.5)ng/mL,显著低于对照组【(73.3±9.2)ng/mL,P<0.05】,血清ADMA水平为(1.8±0.3)μmol/mL,显著低于对照组【(2.2±0.5)μmol/mL,P<0.05】,而血清APN水平为(14.6±6.2)ng/mL,显著高于对照组【(11.3±4.9)ng/mL,P<0.05】。结论应用非诺贝特联合热量限制饮食可降低NAFLD合并T2DM患者血清HCGP39和ADMA水平,而提高血清APN水平,可能与改善了IR,进而改善了肝功能和糖脂代谢有关。展开更多
本文以L-硒代胱氨酸为原料合成了L-硒-甲基硒代半胱氨酸(L-MSC),结构经1H NMR、13 C NMR、HRMS和单晶X射线衍射(XRD)表征。L-MSC与甲硫氨酸氨肽酶2(MetAP2)5D6E蛋白的分子对接结果显示,两者具有较好的氢键作用,且两个氢键与ASP251残基...本文以L-硒代胱氨酸为原料合成了L-硒-甲基硒代半胱氨酸(L-MSC),结构经1H NMR、13 C NMR、HRMS和单晶X射线衍射(XRD)表征。L-MSC与甲硫氨酸氨肽酶2(MetAP2)5D6E蛋白的分子对接结果显示,两者具有较好的氢键作用,且两个氢键与ASP251残基形成键角为72.71°的六元刚性船式构象,L-MSC对HEPG2,SGC790和MCF7细胞的体外增殖抑制活性呈剂量依赖关系,在100μM浓度时的抑制率%分别为72.80±1.21、29.32±1.36和39.54±1.62(CCDC:1870311)。展开更多
Objective:To observe the effects of electroacupuncture(EA)on the protein and gene expressions of Bax,Caspase-3 and Bcl-2 in cerebral cortex of type 2 diabetic rats with cognitive impairment(CI),and to explore the mech...Objective:To observe the effects of electroacupuncture(EA)on the protein and gene expressions of Bax,Caspase-3 and Bcl-2 in cerebral cortex of type 2 diabetic rats with cognitive impairment(CI),and to explore the mechanism of EA in improving the learning and memory abilities.Methods:A total of 100 Sprague-Dawley(SD)rats were divided into a normal group(n=10)and a model group(n=90)by random number table method.Rats in the model group were intraperitoneally injected with a small dose of streptozotocin(STZ)to establish the type 2 diabetic models,after being fed with high-fat and high-sugar diet for 1 month.Twenty CI rats were selected from the 50 successful model rats by the Morris water maze(MWM)test and randomly divided into a model group and an EA group according to the blood glucose level and MWM data(n=10).Rats in the EA group received acupuncture at Zusanli(ST 36),Neiting(ST 44)and Yishu(Extra),of which Zusanli(ST 36)and Neiting(ST 44)were stimulated by EA apparatus,20 min/time,once a day for 6 d a week and 4 consecutive weeks.The rats in the model and the normal groups were fixed without treatment.After 4-week treatment,the random blood glucose level of the rats was measured;the learning and memory abilities of rats were measured by MWM;terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling(TUNEL)assay was used to detect apoptotic cells;Western blot(WB)and real-time quantitative polymerase chain reaction(RT-qPCR)were used to detect the protein and gene expressions of Bax,Caspase-3 and Bcl-2 in cerebral cortex.Results:After modeling,the random blood glucose level and the escape latency tested by MWM were significantly increased,and the number of crossing the platform tested by the MWM was decreased in the EA and model groups,and were significantly different from those in the normal group(P<0.05 or P<0.01),while the differences between the model group and the EA group were not statistically significant(all P>0.05).After 4-week treatment,the random glucose level and the escape latency tested by MWM were significantly increased(both P<0.05),and the number of crossing the original platform tested by the MWM was significantly reduced(P<0.01),the protein and gene expressions of Bax and Caspase-3 were significantly increased(all P<0.001),the protein and gene expressions of Bcl-2 were significantly reduced(both P<0.001),and the number of neuron apoptosis was significantly increased(P<0.001)in the model group than in the normal group;the random blood glucose level was significantly reduced(P<0.05),the escape latency tested by MWM was significantly shortened(P<0.05),and the number of crossing the original platform tested by MWM was significantly increased(P<0.05),the protein and gene expressions of Bax and Caspase-3 were significantly reduced(all P<0.001),the protein and gene expressions of Bcl-2 were significantly increased(both P<0.001),and the number of neuron apoptosis was significantly reduced(P<0.001)in the EA group than in the model group.Conclusion:EA can improve the learning and memory damages induced by type 2 diabetic model rats with CI;the action mechanism may be achieved via anti-apoptosis.展开更多
文摘目的探讨应用非诺贝特联合热量限制饮食治疗非酒精性脂肪性肝病(NAFLD)合并2型糖尿病(T2DM)患者的疗效及患者血清人类软骨糖蛋白-39(HCGP39)、脂联素(APN)和非对称二甲基精氨酸(ADMA)水平的变化。方法将128例NAFLD合并T2DM患者随机分为对照组64例和观察组64例,分别采用热量限制饮食或在此基础上给予非诺贝特口服治疗、观察12周。采用免疫层析法检测血清糖化血红蛋白(HbA1c)水平,采用葡萄糖氧化酶法检测血清空腹血糖(FPG)和餐后2h血糖(2 h PPG)水平,采用磁微粒化学发光法检测血清空腹胰岛素(FINS)水平,并计算胰岛素抵抗(IR)指数。采用ELISA法检测血清HCGP39、APN和ADMA水平。结果在治疗结束时,观察组血清谷丙转氨酶(ALT)水平为(39.4±5.3)U/L,显著低于对照组【(46.8±7.9)U/L,P<0.05】,血清谷草转氨酶(AST)水平为(46.3±4.4)U/L,显著低于对照组【(54.7±7.5)U/L,P<0.05】,血清谷氨酰转移酶(GGT)水平为(33.9±8.6)U/L,显著低于对照组【(43.7±14.6)U/L,P<0.05】;血清FBG水平为(5.9±1.9)mmol/L,显著低于对照组【(6.8±2.0)mmol/L,P<0.05】,血清2 h PPG水平为(7.4±2.2)mmol/L,显著低于对照组【(8.5±2.4)mmol/L,P<0.05】,IR水平为(4.1±0.4),显著低于对照组【(4.7±0.5),P<0.05】;血清总胆固醇(TC)水平为(1.5±0.2)mmol/L,显著低于对照组【(2.1±0.2)mmol/L,P<0.05】,血清三酰甘油(TG)水平为(4.0±0.7)mmol/L,显著低于对照组【(5.5±0.8)mmol/L,P<0.05】,血清低密度脂蛋白(LDL)水平为(2.1±0.5)mmol/L,显著低于对照组【(2.5±0.6)mmol/L,P<0.05】,而血清高密度脂蛋白(HDL)水平为(1.6±0.5)mmol/L,显著高于对照组【(1.4±0.3)mmol/L,P<0.05】;血清HCGP39水平为(61.4±7.5)ng/mL,显著低于对照组【(73.3±9.2)ng/mL,P<0.05】,血清ADMA水平为(1.8±0.3)μmol/mL,显著低于对照组【(2.2±0.5)μmol/mL,P<0.05】,而血清APN水平为(14.6±6.2)ng/mL,显著高于对照组【(11.3±4.9)ng/mL,P<0.05】。结论应用非诺贝特联合热量限制饮食可降低NAFLD合并T2DM患者血清HCGP39和ADMA水平,而提高血清APN水平,可能与改善了IR,进而改善了肝功能和糖脂代谢有关。
文摘本文以L-硒代胱氨酸为原料合成了L-硒-甲基硒代半胱氨酸(L-MSC),结构经1H NMR、13 C NMR、HRMS和单晶X射线衍射(XRD)表征。L-MSC与甲硫氨酸氨肽酶2(MetAP2)5D6E蛋白的分子对接结果显示,两者具有较好的氢键作用,且两个氢键与ASP251残基形成键角为72.71°的六元刚性船式构象,L-MSC对HEPG2,SGC790和MCF7细胞的体外增殖抑制活性呈剂量依赖关系,在100μM浓度时的抑制率%分别为72.80±1.21、29.32±1.36和39.54±1.62(CCDC:1870311)。
文摘Objective:To observe the effects of electroacupuncture(EA)on the protein and gene expressions of Bax,Caspase-3 and Bcl-2 in cerebral cortex of type 2 diabetic rats with cognitive impairment(CI),and to explore the mechanism of EA in improving the learning and memory abilities.Methods:A total of 100 Sprague-Dawley(SD)rats were divided into a normal group(n=10)and a model group(n=90)by random number table method.Rats in the model group were intraperitoneally injected with a small dose of streptozotocin(STZ)to establish the type 2 diabetic models,after being fed with high-fat and high-sugar diet for 1 month.Twenty CI rats were selected from the 50 successful model rats by the Morris water maze(MWM)test and randomly divided into a model group and an EA group according to the blood glucose level and MWM data(n=10).Rats in the EA group received acupuncture at Zusanli(ST 36),Neiting(ST 44)and Yishu(Extra),of which Zusanli(ST 36)and Neiting(ST 44)were stimulated by EA apparatus,20 min/time,once a day for 6 d a week and 4 consecutive weeks.The rats in the model and the normal groups were fixed without treatment.After 4-week treatment,the random blood glucose level of the rats was measured;the learning and memory abilities of rats were measured by MWM;terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling(TUNEL)assay was used to detect apoptotic cells;Western blot(WB)and real-time quantitative polymerase chain reaction(RT-qPCR)were used to detect the protein and gene expressions of Bax,Caspase-3 and Bcl-2 in cerebral cortex.Results:After modeling,the random blood glucose level and the escape latency tested by MWM were significantly increased,and the number of crossing the platform tested by the MWM was decreased in the EA and model groups,and were significantly different from those in the normal group(P<0.05 or P<0.01),while the differences between the model group and the EA group were not statistically significant(all P>0.05).After 4-week treatment,the random glucose level and the escape latency tested by MWM were significantly increased(both P<0.05),and the number of crossing the original platform tested by the MWM was significantly reduced(P<0.01),the protein and gene expressions of Bax and Caspase-3 were significantly increased(all P<0.001),the protein and gene expressions of Bcl-2 were significantly reduced(both P<0.001),and the number of neuron apoptosis was significantly increased(P<0.001)in the model group than in the normal group;the random blood glucose level was significantly reduced(P<0.05),the escape latency tested by MWM was significantly shortened(P<0.05),and the number of crossing the original platform tested by MWM was significantly increased(P<0.05),the protein and gene expressions of Bax and Caspase-3 were significantly reduced(all P<0.001),the protein and gene expressions of Bcl-2 were significantly increased(both P<0.001),and the number of neuron apoptosis was significantly reduced(P<0.001)in the EA group than in the model group.Conclusion:EA can improve the learning and memory damages induced by type 2 diabetic model rats with CI;the action mechanism may be achieved via anti-apoptosis.
