Objective:To investigate the effect of shikonin on the proliferation, apoptosis and extracellular matrix (ECM) of human mesangial cells (MC). Methods: MC was cultured in vitro with different concentrations of glucose ...Objective:To investigate the effect of shikonin on the proliferation, apoptosis and extracellular matrix (ECM) of human mesangial cells (MC). Methods: MC was cultured in vitro with different concentrations of glucose (30, 50, 80 mmol/L). The cell growth was observed by using MTT method and apoptosis by using an aunexin-V-Fluos. Immunohistochemical studies for Laminin (LN), Fibronectin (FN) and type Ⅳ Collagens (Col Ⅳ) were measured. Results: Shikonin inhibited their growth (P<0.05) and apoptosis in the glycated cultured cells. Shikonin 0.05 mmol/L significantly reduced the secretion of LN, FN and Col Ⅳ from MC (P<0.05) cultured in 30, 50 and 80 mmol/L glucose. Conclusion: Shikonin could prevent or treat diabetic nephropathy (DN) and glomerulosclerosis (GS).展开更多
We report a case of gastric choriocarcinoma admixed with an α-fetoprotein (AFP)-producing adeno-carcinoma. A 70-year-old man was hospitalized for gastric cancer that was detected during screening by esophagogastroduo...We report a case of gastric choriocarcinoma admixed with an α-fetoprotein (AFP)-producing adeno-carcinoma. A 70-year-old man was hospitalized for gastric cancer that was detected during screening by esophagogastroduodenoscopy (EGD). Initial laboratory data showed the increased serum level of AFP and EGD revealed a 5-cm ulcerofungating mass in the greater curvature of the gastric antrum. The patient underwent radical subtotal gastrectomywith D2 lymph node dissection and Billroth gastrojejunostomy. Histopathological evaluation confirmed double primary gastric cancer: gastric choriocarcinoma admixed with an AFP-producing adenocarcinoma and separated adenocarcinoma. At 2 wk postoperatively, his human chorionic gonadotropin and AFP levels had reduced and six cycles of adjuvant chemotherapy were initiated. No recurrence or distant metastasis was observed at 4 years postoperatively.展开更多
OBJECTIVE:To study the effect of puerarin on matrix metalloproteinase-2(MMP-2)gene and protein expression in human fetal scleral fibroblasts(HFSFs)exposed to extremely low frequency electromagnetic fields(ELF-EMF).MET...OBJECTIVE:To study the effect of puerarin on matrix metalloproteinase-2(MMP-2)gene and protein expression in human fetal scleral fibroblasts(HFSFs)exposed to extremely low frequency electromagnetic fields(ELF-EMF).METHODS:Cultured HFSFs were exposed to 0.2mT ELF-EMF for 24 h.The experimental groups were divided into subgroups treated with 0,0.1,1and 10μM puerarin respectively.The expression of MMP-2 mRNA and protein were detected with real-time polymerase chain reaction and western-blot analysis respectively.RESULTS:MMP-2 mRNA and protein expression increased by 0.793 and 1.130 folds respectively under the exposure of ELF-EMFs at 0.2 mT flux density for24 h.Puerarin at the concentration of 0.1μM reversed this effect by 8.53%in mRNA and by 17.97%in protein expression(P<0.05).The effect was more prominent at higher concentrations(1 and 10μM,P<0.01).CONCLUSION:Exposure to ELF-EMFs increased the expression of MMP-2 mRNA and protein in HFSF cells.Puerarin reversed the action to some extent in a specific concentration range.Our results implied that the puerarin might protect scleral tissue from increased expression induced by exposure to ELF-EMFs.展开更多
文摘Objective:To investigate the effect of shikonin on the proliferation, apoptosis and extracellular matrix (ECM) of human mesangial cells (MC). Methods: MC was cultured in vitro with different concentrations of glucose (30, 50, 80 mmol/L). The cell growth was observed by using MTT method and apoptosis by using an aunexin-V-Fluos. Immunohistochemical studies for Laminin (LN), Fibronectin (FN) and type Ⅳ Collagens (Col Ⅳ) were measured. Results: Shikonin inhibited their growth (P<0.05) and apoptosis in the glycated cultured cells. Shikonin 0.05 mmol/L significantly reduced the secretion of LN, FN and Col Ⅳ from MC (P<0.05) cultured in 30, 50 and 80 mmol/L glucose. Conclusion: Shikonin could prevent or treat diabetic nephropathy (DN) and glomerulosclerosis (GS).
文摘We report a case of gastric choriocarcinoma admixed with an α-fetoprotein (AFP)-producing adeno-carcinoma. A 70-year-old man was hospitalized for gastric cancer that was detected during screening by esophagogastroduodenoscopy (EGD). Initial laboratory data showed the increased serum level of AFP and EGD revealed a 5-cm ulcerofungating mass in the greater curvature of the gastric antrum. The patient underwent radical subtotal gastrectomywith D2 lymph node dissection and Billroth gastrojejunostomy. Histopathological evaluation confirmed double primary gastric cancer: gastric choriocarcinoma admixed with an AFP-producing adenocarcinoma and separated adenocarcinoma. At 2 wk postoperatively, his human chorionic gonadotropin and AFP levels had reduced and six cycles of adjuvant chemotherapy were initiated. No recurrence or distant metastasis was observed at 4 years postoperatively.
基金Supported by the General Program of the Bio-medical Division of the Shanghai Science and Technology Commission(10411966200)the Scientific Research Fund of Chinese Medical of Shanghai Health Bureau(2009s023)the Shanghai Leading Academic Discipline Project(S30205)
文摘OBJECTIVE:To study the effect of puerarin on matrix metalloproteinase-2(MMP-2)gene and protein expression in human fetal scleral fibroblasts(HFSFs)exposed to extremely low frequency electromagnetic fields(ELF-EMF).METHODS:Cultured HFSFs were exposed to 0.2mT ELF-EMF for 24 h.The experimental groups were divided into subgroups treated with 0,0.1,1and 10μM puerarin respectively.The expression of MMP-2 mRNA and protein were detected with real-time polymerase chain reaction and western-blot analysis respectively.RESULTS:MMP-2 mRNA and protein expression increased by 0.793 and 1.130 folds respectively under the exposure of ELF-EMFs at 0.2 mT flux density for24 h.Puerarin at the concentration of 0.1μM reversed this effect by 8.53%in mRNA and by 17.97%in protein expression(P<0.05).The effect was more prominent at higher concentrations(1 and 10μM,P<0.01).CONCLUSION:Exposure to ELF-EMFs increased the expression of MMP-2 mRNA and protein in HFSF cells.Puerarin reversed the action to some extent in a specific concentration range.Our results implied that the puerarin might protect scleral tissue from increased expression induced by exposure to ELF-EMFs.
