期刊文献+
共找到12篇文章
< 1 >
每页显示 20 50 100
软骨细胞单细胞转录组数据的再挖掘揭示人骨关节炎病程中转录调控因子和代谢的时序变化
1
作者 施剑明 杜桂花 《南昌大学学报(医学版)》 2023年第5期8-14,23,共8页
目的基于单细胞测序(single-cell RNA sequencing,scRNA-seq)数据再挖掘分析探讨人骨关节炎(human osteoarthritis,hOA)进展中相关基因及生物学过程变化。方法从GEO数据库下载hOA软骨细胞scRNA-seq数据集,并使用R软件中Seurat、Monocle ... 目的基于单细胞测序(single-cell RNA sequencing,scRNA-seq)数据再挖掘分析探讨人骨关节炎(human osteoarthritis,hOA)进展中相关基因及生物学过程变化。方法从GEO数据库下载hOA软骨细胞scRNA-seq数据集,并使用R软件中Seurat、Monocle 2和CellCycleSoring等软件包对数据进行质量控制、标准化和归一化,并进行差异基因(特别是转录调控因子)、细胞周期及拟时轨迹分析,在此基础上对差异基因进行基因功能分析并解析涉及的生物学过程。结果scRNA-seq数据分析表明hOA发展初期存在细胞因子的产生和一氧化氮的生物合成等,晚期则伴随血管生成、胶原纤维形成和基质破坏,而细胞炎症反应伴随整个病理过程;差异基因分析筛选出多个新的随hOA进展发生表达水平动态改变的转录调控因子,如KMT2C、EGR2和EBF1;差异基因功能分析显示hOA进展伴随着软骨细胞的代谢改变,表现为氧化磷酸化相关基因表达水平增加,而糖酵解相关基因表达水平降低。结论基于hOA分级的软骨细胞拟时轨迹分析发现hOA进展过程中发生了包括转录调控因子在内的差异基因表达、生物学过程和细胞代谢方式的改变,可为临床开展阶段针对性临床干预和阻遏或逆转hOA进展的新疗法的开发提供了理论基础。 展开更多
关键词 单细胞测序 人骨关节炎 软骨细胞 转录因子 细胞代谢
下载PDF
化痰除湿祛瘀剂对人骨关节炎软骨细胞凋亡的影响 被引量:4
2
作者 梁国强 张慰 +2 位作者 马奇翰 尢君怡 孟祥奇 《中华中医药学刊》 CAS 北大核心 2017年第6期1597-1600,I0035,共5页
目的:研究化痰除湿祛瘀剂对人骨关节炎(osteoarthritis,OA)软骨细胞凋亡的影响。方法:(1)在苏州中医院骨伤科协助下,取因外伤急性截肢患者的软骨组织以及全膝关节置换手术OA患者的软骨组织,采用酶消化培养法培养人软骨细胞。采用甲苯胺... 目的:研究化痰除湿祛瘀剂对人骨关节炎(osteoarthritis,OA)软骨细胞凋亡的影响。方法:(1)在苏州中医院骨伤科协助下,取因外伤急性截肢患者的软骨组织以及全膝关节置换手术OA患者的软骨组织,采用酶消化培养法培养人软骨细胞。采用甲苯胺蓝染色鉴定细胞来源,观察显微镜观察其形态变化。(2)将软骨细胞分成OA软骨细胞组、OA软骨细胞组+化痰除湿祛瘀剂(0.016、0.08、0.4、2、10 mg/mL)组,根据分组情况分别培养24 h或48 h后,应用MTT法比较各组细胞的增殖情况。(3)根据增殖结果将软骨细胞分成OA软骨细胞组、OA软骨细胞组+化痰除湿祛瘀剂(0.05、0.1、2 mg/mL)组,用原位末端标记法(TUNEL)检测各组细胞凋亡情况。结果:(1)成功建立了人骨关节炎软骨细胞有限细胞系,细胞生长状态良好;甲苯胺蓝证明所培养的细胞为来自中胚层的软骨细胞。(2)与对照组比较(24 h+),不同浓度的化痰除湿祛瘀剂对OA软骨细胞活力无影响。与对照组(OD值:0.393±0.010,λ=450 nm)比较(24 h+),化痰除湿祛瘀剂(1 mg/mL,OD值:0.441±0.009)和(2mg/mL,OD值:0.430±0.012)组可明显促进软骨细胞增殖(均P<0.001)。(3)OA软骨细胞经TUNEL标记后的结果显示,对照组的凋亡指数为(42.21±2.67)%,化痰除湿祛瘀剂(0.1 mg/mL,37.14%±4.01%;2 mg/mL,34.21%±5.00%)可明显抑制软骨细胞早期凋亡与对照组比较(P<0.05或P<0.01)。结论:化痰除湿祛瘀剂促进细胞增殖,抑制软骨细胞过度凋亡,这可能是抑制软骨破坏的重要机制之一。 展开更多
关键词 化痰除湿祛瘀剂 人骨关节炎 细胞凋亡
下载PDF
染料木素对人骨关节炎软骨细胞增殖活性和蛋白分泌的影响 被引量:6
3
作者 胡炯 王国强 +2 位作者 刘启明 董黎强 罗统富 《实用药物与临床》 CAS 2018年第7期737-740,共4页
目的探讨染料木素在延缓骨关节炎关节软骨退变中对软骨细胞增殖活性以及分泌Ⅱ型胶原蛋白(CollagenⅡ)和基质金属蛋白酶-13(MMP-13)的影响。方法用白细胞介素1β(IL-1β)处理正常人软骨细胞形成骨关节炎模型,用染料木素及戊酸雌二醇处... 目的探讨染料木素在延缓骨关节炎关节软骨退变中对软骨细胞增殖活性以及分泌Ⅱ型胶原蛋白(CollagenⅡ)和基质金属蛋白酶-13(MMP-13)的影响。方法用白细胞介素1β(IL-1β)处理正常人软骨细胞形成骨关节炎模型,用染料木素及戊酸雌二醇处理骨关节炎软骨细胞,将细胞分为6组,A组:软骨细胞组(对照组);B组:软骨细胞+IL-1β处理组;C组:软骨细胞+IL-1β+染料木素(25μg/ml)处理组;D组:软骨细胞+IL-1β+染料木素(50μg/ml)处理组;E组:软骨细胞+IL-1β+染料木素(100μg/ml)处理组;F组:软骨细胞+IL-1β+戊酸雌二醇(100μg/ml)处理组。CCK-8法检测细胞活力,Western blot检测各组collagenⅡ、MMP-13蛋白水平。结果①甲苯胺蓝染色显示,培养的软骨细胞呈异染性,证实为人软骨细胞。②正常人软骨细胞经浓度10ng/ml的IL-1β处理后,Western blot检测显示,MMP-13蛋白含量升高,collagenⅡ蛋白含量降低,差异有统计学意义(P<0.01),证实此种骨关节炎模型造模方法可行。③染料木素能显著增强骨关节炎软骨细胞的增殖活力,且与时间呈正相关,尤其是在72 h时,浓度为100μg/ml的染料木素对关节炎软骨细胞的增殖活性促进作用最强,差异有统计学意义(P<0.05)。④料木素能显著升高骨关节炎软骨细胞表达collagenⅡ的水平,降低MMP-13的水平。结论浓度为100μg/ml的染料木素处理关节炎软骨细胞在72 h时对细胞作用最为显著,能够增强软骨细胞活力,同时刺激collagenⅡ的合成分泌,抑制MMP-13的表达。 展开更多
关键词 染料木素 人骨关节炎软骨细胞 增殖活性 Ⅱ型胶原蛋白 基质金属蛋白酶-13
下载PDF
白芍总苷对骨关节炎软骨细胞增殖及分泌表达的影响 被引量:30
4
作者 杜旭召 杨豪 +2 位作者 邓素玲 史栋梁 孟庆良 《中国骨质疏松杂志》 CAS CSCD 北大核心 2016年第11期1375-1379,1385,共6页
目的研究白芍总苷(TGP)对人骨关节炎软骨细胞(HOCs)增殖、凋亡、分泌表达的影响及其作用机制,为白芍总苷在骨关节炎治疗方面提供初步的实验依据。方法组织块法培养原代人骨关节炎软骨细胞;TGP处理人骨关节炎软骨细胞并培养12、24和48 h... 目的研究白芍总苷(TGP)对人骨关节炎软骨细胞(HOCs)增殖、凋亡、分泌表达的影响及其作用机制,为白芍总苷在骨关节炎治疗方面提供初步的实验依据。方法组织块法培养原代人骨关节炎软骨细胞;TGP处理人骨关节炎软骨细胞并培养12、24和48 h;采用CCK-8检测细胞存活率;ELISA检测炎症因子肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)、白细胞介素-8(IL-8)以及Caspase-3表达水平;Western blotting检测Bcl-2、Bax、基质金属蛋白酶-13(MMP-13)、金属蛋白酶组织抑制剂-1(TIMP-1)和核转录因子κB(NF-κB)的蛋白表达水平。结果 TGP能促进细胞增殖,并存在时间和浓度依赖性,其中8μmol/L TGP作用24 h时细胞存活率最高,存在显著性差异(P<0.05);TGP可以显著抑制凋亡相关蛋白Bax和Caspase-3表达,并促进抗凋亡蛋白Bcl-2表达(P<0.05);TGP可以显著降低与软骨退化相关的MMP-13/TIMP-1比值(P<0.05);TGP可以抑制炎症因子TNF-α、IL-6和IL-8表达(P<0.05);TGP可以抑制NF-κB的表达(P<0.05)。结论 TGP促进人骨关节炎软骨细胞增殖,抑制细胞凋亡、降低MMP-13/TIMP-1比值以及炎症因子的分泌,其机制可能是与抑制NF-κB的表达相关。 展开更多
关键词 中医中药 白芍总苷 人骨关节炎软骨细胞 增殖 分泌表达
下载PDF
lncRNA NEAT1/SFPQ/RUNX2轴研究P-15对软骨损伤的保护作用
5
作者 邓长弓 李源力 +1 位作者 聂君兰 李洪 《中国骨质疏松杂志》 CAS CSCD 北大核心 2023年第12期1750-1755,共6页
目的探究P-15对人骨关节炎软骨细胞(HC-OA)的保护作用及与lncRNA NEAT1/SFPQ/RUNX2轴的关系。方法分离HC-OA胞核、胞质RNA,RT-qPCR检测lncRNA NEAT1在细胞中的分布;P-15处理HC-OA细胞,转染siRNA NC和siRNA SFPQ后,RT-qPCR检测lncRNA NE... 目的探究P-15对人骨关节炎软骨细胞(HC-OA)的保护作用及与lncRNA NEAT1/SFPQ/RUNX2轴的关系。方法分离HC-OA胞核、胞质RNA,RT-qPCR检测lncRNA NEAT1在细胞中的分布;P-15处理HC-OA细胞,转染siRNA NC和siRNA SFPQ后,RT-qPCR检测lncRNA NEAT1表达水平;流式细胞仪检测细胞凋亡;Western blot检测RUNX2、SFPQ、Beclin-1、LC3B和P62表达水平。结果lncRNA NEAT1同时存在于HC-OA胞质和胞核,且更多分布于细胞核;P-15可降低胞内lncRNA NEAT1水平(P<0.05),抑制骨关节炎软骨细胞凋亡(P<0.05);P-15可通过调控lncRNA NEAT1/SFPQ/RUNX2轴上调SFPQ表达和抑制RUNX2表达(P<0.01);P-15调控lncRNA NEAT1/SFPQ/RUNX2轴促进软骨细胞发生自噬(P<0.05)。结论P-15可调控lncRNA NEAT1/SFPQ/RUNX2轴抑制HC-OA凋亡并促进自噬,保护软骨细胞免受损伤。 展开更多
关键词 P-15肽 lncRNA NEAT1/SFPQ/RUNX2轴 人骨关节炎软骨细胞 凋亡 自噬
下载PDF
血清14-3-3η蛋白在类风湿关节炎及骨关节炎患者血清的表达及临床意义 被引量:5
6
作者 何睿妍 张宁 《解剖科学进展》 2018年第1期84-87,共4页
目的检测类风湿关节炎(RA)、骨关节炎(OA)患者血清14-3-3η蛋白水平,探讨其临床意义。方法选取56例RA患者、31例OA患者及31例健康体检者,应用ELISA法测定血清14-3-3η蛋白水平。结果RA组血清14-3-3η蛋白水平高于OA组,OA组高于健康对照... 目的检测类风湿关节炎(RA)、骨关节炎(OA)患者血清14-3-3η蛋白水平,探讨其临床意义。方法选取56例RA患者、31例OA患者及31例健康体检者,应用ELISA法测定血清14-3-3η蛋白水平。结果RA组血清14-3-3η蛋白水平高于OA组,OA组高于健康对照组(P<0.05)。RA组血清14-3-3η蛋白水平与抗CCP抗体、DAS28评分、CRP呈正相关(P<0.05),对RA诊断敏感性为100%,特异性为71%。结论14-3-3η蛋白参与RA的致病过程,反映RA疾病活动度和疾病严重程度,可作为RA的诊断指标之一。 展开更多
关键词 人类风湿关节炎 人骨关节炎 血清 14-3-3η蛋白 酶联免疫吸附试验
原文传递
Influencing factors analysis of spontaneous knee joint osteoarthritis among middle aged and old aged pople in Xi'an 被引量:4
7
作者 王伟 王坤正 +4 位作者 党晓谦 柏传毅 王春生 时志斌 马树强 《Journal of Medical Colleges of PLA(China)》 CAS 2007年第3期179-184,共6页
Objective:To explore the prevalence rate of knee joint osteoarthritis and its pathogenetic fea- tures among the middle-aged and old aged people in the urban and rural area of Xi'an.Methods:From May to October 2005... Objective:To explore the prevalence rate of knee joint osteoarthritis and its pathogenetic fea- tures among the middle-aged and old aged people in the urban and rural area of Xi'an.Methods:From May to October 2005,1538 Han nationality above 40 years in Xi'an were investigated according to strati- fied and cluster random sampling.The same questionnaire was delivered to subjects who had been given normotopia and lateral position radiographic examination in both knees.Excluded from etiological arthri- tis,some subjects with clinical symptoms and a radiographic grade (beyond Kellgren & Lawrence gradeⅡ) were finally diagnosed as spontaneous knee joint osteoarthritis.All analyses were performed with SPSS 13.0 and t test,U test.Single factor analysis and multiple logistic regression analysis were used to analyze dates.Results:The total prevalence of spontaneous knee joint osteoarthritis was 12.1% with 2.5% and 2.1% for right and left knees,respectively.The prevalence of knee joint osteoarthritis in wom- en was 2.5 times of that in men (17.2% vs 6.8%,P=0.000).the osteophyte prevalence in women was 2.3 times of that in men (26.5% vs 11.4%,P=0.000).The prevalence of knee joint osteoarthritis and osteophyte increased with the growth of age.So did the symptomatic knee,except for the age group of 56- 60 years and 66-70 years.The prevalence of symptomatic knee joint for urban area was significantly higher than that for rural area (56.3% vs 45.3%,P=0.003).In women,the prevalence of knee joint os- teoarthritis increased significantly with the increasing of body mass index,which had not been observed in men.Female and obesity are the risk factors for osteoarthritis,but bean and its products and meat could prevent the osteoarthritis.Conclusion:Old age,female and obesity serve as risk factors for knee joint os- teoarthritis. 展开更多
关键词 OSTEOARTHRITIS KNEE SPONTANEOUS EPIDEMIOLOGY middle and old aged
下载PDF
Treatment of Genual Osteoarthritis by Massotherapy
8
作者 陈元戈 《Journal of Traditional Chinese Medicine》 SCIE CAS CSCD 2000年第3期191-194,共4页
Genual osteoarthritis is a commonly-encountered affection in the middle-aged and oldpersons.The author has treated 65 cases of genualosteoarthritis with massotherapy in the past threeyears,with satisfactory therapeuti... Genual osteoarthritis is a commonly-encountered affection in the middle-aged and oldpersons.The author has treated 65 cases of genualosteoarthritis with massotherapy in the past threeyears,with satisfactory therapeutic results reported asfollows. 展开更多
关键词 MASSAGE ADULT Aged Aged 80 and over FEMALE Humans MALE Middle Aged Osteoarthritis Knee
下载PDF
Treatment of Obstinate Diseases by Acupuncture and Cupping
9
作者 杨容 《Journal of Traditional Chinese Medicine》 SCIE CAS CSCD 2001年第2期118-121,共4页
Acupuncture treatment integrated with cupping for enhancing the therapeutic effect is a technique in Yang's school of acupuncture carried forward from generation to generation. The author's grandfather created... Acupuncture treatment integrated with cupping for enhancing the therapeutic effect is a technique in Yang's school of acupuncture carried forward from generation to generation. The author's grandfather created Yang's shallow puncturing and cupping therapy to enhance the effect of promoting blood flow and removing blood stasis in the light of his clinical experience. In this therapy, the gentle puncturing and heavy tapping are carried out with the seven-star needle until light bleeding occurs, followed by drawing out of pituita or stagnant blood and coagula by cupping, in an attempt to promote tissue regeneration by removing blood stasis for promoting the flow of qi and blood in the meridians. The therapy has been developed by Dr. Yang Yifang (the author's father) in clinical practice over the past six decades. Satisfactory therapeutic results have been obtained in clinical practice, which are introduced with examples below. 展开更多
关键词 Acupuncture Therapy ADULT Facial Paralysis FEMALE Humans Male Middle Aged OSTEOARTHRITIS URTICARIA
下载PDF
Clinical Effect of Acupuncture Treatment in 109 Cases of Knee Osteoarthritis
10
作者 姜爱平 张磊 +2 位作者 赵长信 杨福贤 范玲玲 《Journal of Traditional Chinese Medicine》 SCIE CAS CSCD 2001年第4期282-285,共4页
Knee Osteoarthritis, a retrograde disease, is mainly characterized by pain in the knee joints and limitation of the movement. It is a common and frequently occurring disease affecting the health and life quality of ol... Knee Osteoarthritis, a retrograde disease, is mainly characterized by pain in the knee joints and limitation of the movement. It is a common and frequently occurring disease affecting the health and life quality of old people. In recent years, acupuncture treatment of knee osteoarthritis with satisfactory therapeutic results has been repeatedly reported,2-3, 5-6 but there has been few report on the observation of long-term therapeutic effects after treatment. With acupuncture as the main therapy, 109 cases of knee osteoarthritis were treated in this series. 91 cases with good therapeutic results were followed up for 6 months. A report is presented in the following. 展开更多
关键词 Acupuncture Therapy ADULT Aged Aged 80 and over FEMALE Follow-Up Studies Humans Male Middle Aged Osteoarthritis Knee RECURRENCE
下载PDF
Forty-seven Cases of Gonitis Treated by A Combined Therapy of Chinese Drugs and Acupuncture
11
作者 杨文鹤 《Journal of Traditional Chinese Medicine》 SCIE CAS CSCD 2001年第2期127-129,共3页
Osteoarthritis, a nonspecific inflammatory lesion, is a commonly seen joint disease. Clinically, it is mainly characterized by arthralgia, swelling, and motor impairment. Since the knee joint is the load-bearing joint... Osteoarthritis, a nonspecific inflammatory lesion, is a commonly seen joint disease. Clinically, it is mainly characterized by arthralgia, swelling, and motor impairment. Since the knee joint is the load-bearing joint of the human body, and is susceptible to trauma, gonitis tends to have the highest morbidity in the four limbs, which manifests itself arthritis of patella and femur at the early stage; narrowness or disappearance of the medial joint space at the mid stage; and damage of the cartilage accompanied with flexion deformity at the late stage. In recent years, based on the experience of Prof. Cao Yiming, the author has treated 47 cases of gonitis by combined use of Chinese drugs and acupuncture, and obtained satisfactory therapeutic results as reported in the following. 展开更多
关键词 Acupuncture Therapy ADULT Aged Aged 80 and over Combined Modality Therapy Drugs Chinese Herbal Female Humans Male Middle Aged Osteoarthritis Knee
下载PDF
Observation on the Therapeutic Effect of Shu Feng Huo Luo Pian for Treatment of Osseous Arthritis
12
作者 吴晓松 周毅 王新中 《Journal of Traditional Chinese Medicine》 SCIE CAS CSCD 2002年第1期12-14,共3页
OBJECTIVE: To evaluate the therapeutic effect of Chinese patent drug Shu Feng Huo Luo Pian ([symbol: see text] Pill for Dispelling Wind and Activating Collaterals) for the treatment of osseous arthritis. METHOD: 50 ca... OBJECTIVE: To evaluate the therapeutic effect of Chinese patent drug Shu Feng Huo Luo Pian ([symbol: see text] Pill for Dispelling Wind and Activating Collaterals) for the treatment of osseous arthritis. METHOD: 50 cases of osseous arthritis were divided randomly into two groups, the Shu Feng Huo Luo Pian group (the experimental group), including 30 cases (aged 63.5 +/- 4 years), treated with 2 such pills, p.o., bid; and the control group, including 19 cases (aged 63 +/- 5 years), treated with Sulindac 0.2 g, p.o., bid. The two groups were all supplemented by medication of calcium Caltrate D 0.6 g, p.o., qd. The above-mentioned medications were administered for 2 courses, 2 weeks constituting a course. RESULT: The total effective rate of the experimental group evaluated by the doctors was 83.3%, and that evaluated by the patients was 90%, with mild side effects. CONCLUSION: Shu Feng Huo Luo Pian is an effective Chinese patent drug for treating osseous arthritis, with less and mild side effects. 展开更多
关键词 Aged Calcium Drug Combinations Drug Therapy Combination Drugs Chinese Herbal Female Humans Male Middle Aged OSTEOARTHRITIS SULINDAC TABLETS Vitamin D
下载PDF
上一页 1 下一页 到第
使用帮助 返回顶部