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白细胞介素-37b负反馈调控Toll样受体4信号通路对的人骨性关节炎的保护作用研究 被引量:2
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作者 王文靖 耿萍 +4 位作者 李旻 廖辉雄 郭方舟 杨颖 袁志刚 《中国临床药理学杂志》 CAS CSCD 北大核心 2020年第19期3026-3029,共4页
目的探讨白细胞介素-37b(IL-37b)负反馈调控Toll样受体4(TLR4)信号通路对人骨性关节炎(OA)的保护机制。方法常规培养OA软骨细胞,分别用100 nmol·L^-1的pEGFP-IL-37b、pEGFP-IL-37b-NC1、IL-37b-siRNA及IL-37b-siRNA-NC2转染至细胞... 目的探讨白细胞介素-37b(IL-37b)负反馈调控Toll样受体4(TLR4)信号通路对人骨性关节炎(OA)的保护机制。方法常规培养OA软骨细胞,分别用100 nmol·L^-1的pEGFP-IL-37b、pEGFP-IL-37b-NC1、IL-37b-siRNA及IL-37b-siRNA-NC2转染至细胞,并分别记为B、C、D和E组;另取OA软骨细胞仅只加入Lipofectamine 3000试剂作为A组。用酶联免疫吸附法检测细胞中肿瘤坏死因子-α(TNF-α)和IL^-1β的表达水平,用Western Blotting检测细胞中TLR4、髓样分化因子(MyD88)及核转录因子-κB(NF-κB)蛋白的相对表达水平。结果A,B,C,D和E组的TNF-α分别为(1.01±0),(0.53±0.01),(0.99±0.04),(1.75±0.10)和(1.00±0.01)ng·mL^-1,IL^-1β分别为(0.95±0.03),(0.46±0.13),(0.96±0.05),(1.84±0.09)和(0.96±0.06)ng·mL^-1,TLR4蛋白相对表达量分别为1.00±0.01,0.29±0.04,1.00±0.02,1.81±0.04和1.00±0.01,MyD88蛋白相对表达量分别为1.00±0.02,0.28±0.12,0.96±0.02,1.86±0.07和0.98±0.02,NF-κB蛋白相对表达量分别为0.96±0.03,0.43±0.02,0.97±0.05,1.50±0.05和0.97±0.04,A组的上述指标与B,D组相比,差异均有统计意义(均P<0.05)。结论IL-37b在OA中能够抑制TLR4/MyD88/NF-κB信号通路的表达,从而抑制炎症的发生。 展开更多
关键词 白细胞介素-37b 人骨性关节炎 Toll样受体4/髓样分化因子/核转录因子-κB信号通路
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Influencing factors analysis of spontaneous knee joint osteoarthritis among middle aged and old aged pople in Xi'an 被引量:4
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作者 王伟 王坤正 +4 位作者 党晓谦 柏传毅 王春生 时志斌 马树强 《Journal of Medical Colleges of PLA(China)》 CAS 2007年第3期179-184,共6页
Objective:To explore the prevalence rate of knee joint osteoarthritis and its pathogenetic fea- tures among the middle-aged and old aged people in the urban and rural area of Xi'an.Methods:From May to October 2005... Objective:To explore the prevalence rate of knee joint osteoarthritis and its pathogenetic fea- tures among the middle-aged and old aged people in the urban and rural area of Xi'an.Methods:From May to October 2005,1538 Han nationality above 40 years in Xi'an were investigated according to strati- fied and cluster random sampling.The same questionnaire was delivered to subjects who had been given normotopia and lateral position radiographic examination in both knees.Excluded from etiological arthri- tis,some subjects with clinical symptoms and a radiographic grade (beyond Kellgren & Lawrence gradeⅡ) were finally diagnosed as spontaneous knee joint osteoarthritis.All analyses were performed with SPSS 13.0 and t test,U test.Single factor analysis and multiple logistic regression analysis were used to analyze dates.Results:The total prevalence of spontaneous knee joint osteoarthritis was 12.1% with 2.5% and 2.1% for right and left knees,respectively.The prevalence of knee joint osteoarthritis in wom- en was 2.5 times of that in men (17.2% vs 6.8%,P=0.000).the osteophyte prevalence in women was 2.3 times of that in men (26.5% vs 11.4%,P=0.000).The prevalence of knee joint osteoarthritis and osteophyte increased with the growth of age.So did the symptomatic knee,except for the age group of 56- 60 years and 66-70 years.The prevalence of symptomatic knee joint for urban area was significantly higher than that for rural area (56.3% vs 45.3%,P=0.003).In women,the prevalence of knee joint os- teoarthritis increased significantly with the increasing of body mass index,which had not been observed in men.Female and obesity are the risk factors for osteoarthritis,but bean and its products and meat could prevent the osteoarthritis.Conclusion:Old age,female and obesity serve as risk factors for knee joint os- teoarthritis. 展开更多
关键词 OSTEOARTHRITIS KNEE SPONTANEOUS EPIDEMIOLOGY middle and old aged
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Treatment of Genual Osteoarthritis by Massotherapy
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作者 陈元戈 《Journal of Traditional Chinese Medicine》 SCIE CAS CSCD 2000年第3期191-194,共4页
Genual osteoarthritis is a commonly-encountered affection in the middle-aged and oldpersons.The author has treated 65 cases of genualosteoarthritis with massotherapy in the past threeyears,with satisfactory therapeuti... Genual osteoarthritis is a commonly-encountered affection in the middle-aged and oldpersons.The author has treated 65 cases of genualosteoarthritis with massotherapy in the past threeyears,with satisfactory therapeutic results reported asfollows. 展开更多
关键词 MASSAGE ADULT Aged Aged 80 and over FEMALE Humans MALE Middle Aged Osteoarthritis Knee
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Treatment of Obstinate Diseases by Acupuncture and Cupping
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作者 杨容 《Journal of Traditional Chinese Medicine》 SCIE CAS CSCD 2001年第2期118-121,共4页
Acupuncture treatment integrated with cupping for enhancing the therapeutic effect is a technique in Yang's school of acupuncture carried forward from generation to generation. The author's grandfather created... Acupuncture treatment integrated with cupping for enhancing the therapeutic effect is a technique in Yang's school of acupuncture carried forward from generation to generation. The author's grandfather created Yang's shallow puncturing and cupping therapy to enhance the effect of promoting blood flow and removing blood stasis in the light of his clinical experience. In this therapy, the gentle puncturing and heavy tapping are carried out with the seven-star needle until light bleeding occurs, followed by drawing out of pituita or stagnant blood and coagula by cupping, in an attempt to promote tissue regeneration by removing blood stasis for promoting the flow of qi and blood in the meridians. The therapy has been developed by Dr. Yang Yifang (the author's father) in clinical practice over the past six decades. Satisfactory therapeutic results have been obtained in clinical practice, which are introduced with examples below. 展开更多
关键词 Acupuncture Therapy ADULT Facial Paralysis FEMALE Humans Male Middle Aged OSTEOARTHRITIS URTICARIA
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Clinical Effect of Acupuncture Treatment in 109 Cases of Knee Osteoarthritis
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作者 姜爱平 张磊 +2 位作者 赵长信 杨福贤 范玲玲 《Journal of Traditional Chinese Medicine》 SCIE CAS CSCD 2001年第4期282-285,共4页
Knee Osteoarthritis, a retrograde disease, is mainly characterized by pain in the knee joints and limitation of the movement. It is a common and frequently occurring disease affecting the health and life quality of ol... Knee Osteoarthritis, a retrograde disease, is mainly characterized by pain in the knee joints and limitation of the movement. It is a common and frequently occurring disease affecting the health and life quality of old people. In recent years, acupuncture treatment of knee osteoarthritis with satisfactory therapeutic results has been repeatedly reported,2-3, 5-6 but there has been few report on the observation of long-term therapeutic effects after treatment. With acupuncture as the main therapy, 109 cases of knee osteoarthritis were treated in this series. 91 cases with good therapeutic results were followed up for 6 months. A report is presented in the following. 展开更多
关键词 Acupuncture Therapy ADULT Aged Aged 80 and over FEMALE Follow-Up Studies Humans Male Middle Aged Osteoarthritis Knee RECURRENCE
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Forty-seven Cases of Gonitis Treated by A Combined Therapy of Chinese Drugs and Acupuncture
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作者 杨文鹤 《Journal of Traditional Chinese Medicine》 SCIE CAS CSCD 2001年第2期127-129,共3页
Osteoarthritis, a nonspecific inflammatory lesion, is a commonly seen joint disease. Clinically, it is mainly characterized by arthralgia, swelling, and motor impairment. Since the knee joint is the load-bearing joint... Osteoarthritis, a nonspecific inflammatory lesion, is a commonly seen joint disease. Clinically, it is mainly characterized by arthralgia, swelling, and motor impairment. Since the knee joint is the load-bearing joint of the human body, and is susceptible to trauma, gonitis tends to have the highest morbidity in the four limbs, which manifests itself arthritis of patella and femur at the early stage; narrowness or disappearance of the medial joint space at the mid stage; and damage of the cartilage accompanied with flexion deformity at the late stage. In recent years, based on the experience of Prof. Cao Yiming, the author has treated 47 cases of gonitis by combined use of Chinese drugs and acupuncture, and obtained satisfactory therapeutic results as reported in the following. 展开更多
关键词 Acupuncture Therapy ADULT Aged Aged 80 and over Combined Modality Therapy Drugs Chinese Herbal Female Humans Male Middle Aged Osteoarthritis Knee
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Observation on the Therapeutic Effect of Shu Feng Huo Luo Pian for Treatment of Osseous Arthritis
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作者 吴晓松 周毅 王新中 《Journal of Traditional Chinese Medicine》 SCIE CAS CSCD 2002年第1期12-14,共3页
OBJECTIVE: To evaluate the therapeutic effect of Chinese patent drug Shu Feng Huo Luo Pian ([symbol: see text] Pill for Dispelling Wind and Activating Collaterals) for the treatment of osseous arthritis. METHOD: 50 ca... OBJECTIVE: To evaluate the therapeutic effect of Chinese patent drug Shu Feng Huo Luo Pian ([symbol: see text] Pill for Dispelling Wind and Activating Collaterals) for the treatment of osseous arthritis. METHOD: 50 cases of osseous arthritis were divided randomly into two groups, the Shu Feng Huo Luo Pian group (the experimental group), including 30 cases (aged 63.5 +/- 4 years), treated with 2 such pills, p.o., bid; and the control group, including 19 cases (aged 63 +/- 5 years), treated with Sulindac 0.2 g, p.o., bid. The two groups were all supplemented by medication of calcium Caltrate D 0.6 g, p.o., qd. The above-mentioned medications were administered for 2 courses, 2 weeks constituting a course. RESULT: The total effective rate of the experimental group evaluated by the doctors was 83.3%, and that evaluated by the patients was 90%, with mild side effects. CONCLUSION: Shu Feng Huo Luo Pian is an effective Chinese patent drug for treating osseous arthritis, with less and mild side effects. 展开更多
关键词 Aged Calcium Drug Combinations Drug Therapy Combination Drugs Chinese Herbal Female Humans Male Middle Aged OSTEOARTHRITIS SULINDAC TABLETS Vitamin D
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