目的探讨血清外泌体源性circ_0009362对人骨髓间充干细胞(human bone marrow mesenchymalstem cells,hBMSCs)成骨分化的影响及潜在作用机制。方法收集骨质疏松症(osteoporosis,OP)患者的血清并分离外泌体,使用qRT-PCR检测外泌体中circ_0...目的探讨血清外泌体源性circ_0009362对人骨髓间充干细胞(human bone marrow mesenchymalstem cells,hBMSCs)成骨分化的影响及潜在作用机制。方法收集骨质疏松症(osteoporosis,OP)患者的血清并分离外泌体,使用qRT-PCR检测外泌体中circ_0009362的表达水平。诱导hBMSCs成骨并检测细胞中circ_0009362、miR-29b-3p的表达。双荧光素酶报告实验检测circ_0009362、miR-29b-3p的相互调控关系。碱性磷酸酶(ALP)试剂盒检测ALP活性,茜素红(ARS)染色法检测钙沉积。结果与健康对照组相比,OP患者的血清外泌体中circ_0009362表达升高,诱导hBMSCs成骨后circ_0009362的表达降低(均P<0.05)。外泌体能降低hBMSCs中ALP活性与钙沉积百分比,该作用通过分泌circ_0009362实现。敲减外泌体中circ_0009362表达能部分抵消外泌体的作用(均P<0.05)。诱导hBMSCs成骨后miR-29b-3p的表达提高(P<0.05)。circ_0009362与miR-29b-3p存在靶向关系,外泌体通过分泌circ_0009362抑制miR-29b-3p的表达。过表达miR-29b-3p促进hBMSCs中ALP活性与钙沉积百分比,该作用被外泌体部分抵消(均P<0.05)。结论OP患者的血清外泌体通过分泌circ_0009362下调miR-29b-3p的表达来抑制hBMSCs成骨分化。展开更多
In order to study the influence of rubidium(Rb)addition on the phase composition,microstructure,mechanical properties and cell response of bioactive glass-ceramics,CaO−SiO2−Na2O−B2O3−MgO−ZnO−P2O5 glass system was desi...In order to study the influence of rubidium(Rb)addition on the phase composition,microstructure,mechanical properties and cell response of bioactive glass-ceramics,CaO−SiO2−Na2O−B2O3−MgO−ZnO−P2O5 glass system was designed with and without addition of Rb.The results show that hydroxyapatite(HA)and Mg−whitelockite(Ca18Mg2H2(PO4)14)crystalline phases are formed in the glass matrix without Rb.After the addition of Rb,only HA phase is detected.The grain size of the crystals in the glass-ceramics is larger with the addition of Rb than that of samples without Rb.Rb addition can improve the bending strength of glass-ceramics.The cultivation of human bone marrow mesenchymal stem cells(hBMSCs)on Rb-containing glass-ceramics demonstrates enhanced cell adhesion,proliferation and ALP activity.In conclusion,Rb-modified glass-ceramics exhibit good mechanical property,excellent bioactivity and biocompatibility,which have potential for bone regeneration application.展开更多
Magnesium alloys show promise for application in orthopedic implants, owing to their biodegradability and biocompatibility. In the present study, ternary Mg-(3.5,6.5 wt%) Li-(0.2, 0.5, 1.0 wt%) Ca alloys were develope...Magnesium alloys show promise for application in orthopedic implants, owing to their biodegradability and biocompatibility. In the present study, ternary Mg-(3.5,6.5 wt%) Li-(0.2, 0.5, 1.0 wt%) Ca alloys were developed. Their mechanical strength, corrosion behavior and cytocompatibility were studied. These alloys showed improved mechanical strength than pure Mg and exhibited suitable corrosion resistance. Furthermore, Mg-3.5Li-0.5Ca alloys with the best in vitro performance were implanted intramedullary into the femurs of mice for 2 and 8 weeks. In vivo results revealed a significant increase in cortical bone thickness around the Mg-3.5Li-0.5Ca alloy rods, without causing any adverse effects.Western blotting and immunofluorescence staining of β-catenin illustrated that Mg-3.5Li-0.5Ca alloy extracts induced osteogenic differentiation of human bone marrow-derived mesenchymal stem cells(hBMMSCs) through the canonical Wnt/β-catenin pathway. Our studies demonstrate that Mg-3.5Li-0.5Ca alloys hold much promise as candidates for the facilitation of bone implant application.展开更多
文摘目的探讨血清外泌体源性circ_0009362对人骨髓间充干细胞(human bone marrow mesenchymalstem cells,hBMSCs)成骨分化的影响及潜在作用机制。方法收集骨质疏松症(osteoporosis,OP)患者的血清并分离外泌体,使用qRT-PCR检测外泌体中circ_0009362的表达水平。诱导hBMSCs成骨并检测细胞中circ_0009362、miR-29b-3p的表达。双荧光素酶报告实验检测circ_0009362、miR-29b-3p的相互调控关系。碱性磷酸酶(ALP)试剂盒检测ALP活性,茜素红(ARS)染色法检测钙沉积。结果与健康对照组相比,OP患者的血清外泌体中circ_0009362表达升高,诱导hBMSCs成骨后circ_0009362的表达降低(均P<0.05)。外泌体能降低hBMSCs中ALP活性与钙沉积百分比,该作用通过分泌circ_0009362实现。敲减外泌体中circ_0009362表达能部分抵消外泌体的作用(均P<0.05)。诱导hBMSCs成骨后miR-29b-3p的表达提高(P<0.05)。circ_0009362与miR-29b-3p存在靶向关系,外泌体通过分泌circ_0009362抑制miR-29b-3p的表达。过表达miR-29b-3p促进hBMSCs中ALP活性与钙沉积百分比,该作用被外泌体部分抵消(均P<0.05)。结论OP患者的血清外泌体通过分泌circ_0009362下调miR-29b-3p的表达来抑制hBMSCs成骨分化。
基金The authors are grateful for the financial supports from the Natural Science Foundation of Hunan Province,China(2019JJ50797)the Postdoctoral Science Foundation of China(2019T120711).
文摘In order to study the influence of rubidium(Rb)addition on the phase composition,microstructure,mechanical properties and cell response of bioactive glass-ceramics,CaO−SiO2−Na2O−B2O3−MgO−ZnO−P2O5 glass system was designed with and without addition of Rb.The results show that hydroxyapatite(HA)and Mg−whitelockite(Ca18Mg2H2(PO4)14)crystalline phases are formed in the glass matrix without Rb.After the addition of Rb,only HA phase is detected.The grain size of the crystals in the glass-ceramics is larger with the addition of Rb than that of samples without Rb.Rb addition can improve the bending strength of glass-ceramics.The cultivation of human bone marrow mesenchymal stem cells(hBMSCs)on Rb-containing glass-ceramics demonstrates enhanced cell adhesion,proliferation and ALP activity.In conclusion,Rb-modified glass-ceramics exhibit good mechanical property,excellent bioactivity and biocompatibility,which have potential for bone regeneration application.
基金supported by the National Key Research and Development Program of China (2016YFC1102900 and 2016YFC1102402)the National Natural Science Foundation of China (81771039, 81470769 and 51431002)+2 种基金the Project for Culturing Leading Talents in Scientific and Technological Innovation of Beijing, China (Z171100001117169)the NSFC-RFBR Cooperation Project (51611130054)the NSFC/RGC Joint Research Scheme (51361165101 and 5161101031)
文摘Magnesium alloys show promise for application in orthopedic implants, owing to their biodegradability and biocompatibility. In the present study, ternary Mg-(3.5,6.5 wt%) Li-(0.2, 0.5, 1.0 wt%) Ca alloys were developed. Their mechanical strength, corrosion behavior and cytocompatibility were studied. These alloys showed improved mechanical strength than pure Mg and exhibited suitable corrosion resistance. Furthermore, Mg-3.5Li-0.5Ca alloys with the best in vitro performance were implanted intramedullary into the femurs of mice for 2 and 8 weeks. In vivo results revealed a significant increase in cortical bone thickness around the Mg-3.5Li-0.5Ca alloy rods, without causing any adverse effects.Western blotting and immunofluorescence staining of β-catenin illustrated that Mg-3.5Li-0.5Ca alloy extracts induced osteogenic differentiation of human bone marrow-derived mesenchymal stem cells(hBMMSCs) through the canonical Wnt/β-catenin pathway. Our studies demonstrate that Mg-3.5Li-0.5Ca alloys hold much promise as candidates for the facilitation of bone implant application.
