An indirect ELISA by using monoclonal antibody (McAb) against hutnan IgM (μ chain)as the antibody labeled with horseradish peroxidase (second antibody) is used in detecion Rubela virus - specific IgM for early diagno...An indirect ELISA by using monoclonal antibody (McAb) against hutnan IgM (μ chain)as the antibody labeled with horseradish peroxidase (second antibody) is used in detecion Rubela virus - specific IgM for early diagnosis of Rubella virus infection. Through the detection and verifition of a large number of sera from normal population, the population closely contacted with Rubella virus and IgM - positive sera samples of Measles virus, cycoegaforirm and Toxplasma Gondii, it is indicated that this method is specific, seusiuive, simple, rapid and no cross - reaction with the positive sera of the pathogens menticned above, It can be used for early diagnosis of Rubella virus infection and epidemiological survey.展开更多
The sera of 180 human samples were tested for the presence of antibodies against Borrelia burgdorferi using the ELISA （enzyme-linked immunosorbent assay） technique. Out of 180 sero-samples, 46 （25.55%） were positi...The sera of 180 human samples were tested for the presence of antibodies against Borrelia burgdorferi using the ELISA （enzyme-linked immunosorbent assay） technique. Out of 180 sero-samples, 46 （25.55%） were positive. Females of the age range 18-35 years had the highest rate of sero-positive samples 14 （38.88%）, while the highest percentage of sero-negative samples was found in males of the age range 50-80 years. The other sero-positive samples were： 6 （26.08%）, 6 （25%） and 3 （11.53%） in males of ages between 18-35, 35-50 and 50-80 years, respectively, and 11 （29.72%） and 6 （17.64%） in females in the age ranges 35-50 and 50-80 years, respectively. The mean concentration of Anti- B. burgdorferi antibody was higher （16.7 U/mL） when compared with mean concentration of normal value （5.5 U/mL）, P 〈 0.001.展开更多
文摘An indirect ELISA by using monoclonal antibody (McAb) against hutnan IgM (μ chain)as the antibody labeled with horseradish peroxidase (second antibody) is used in detecion Rubela virus - specific IgM for early diagnosis of Rubella virus infection. Through the detection and verifition of a large number of sera from normal population, the population closely contacted with Rubella virus and IgM - positive sera samples of Measles virus, cycoegaforirm and Toxplasma Gondii, it is indicated that this method is specific, seusiuive, simple, rapid and no cross - reaction with the positive sera of the pathogens menticned above, It can be used for early diagnosis of Rubella virus infection and epidemiological survey.
文摘The sera of 180 human samples were tested for the presence of antibodies against Borrelia burgdorferi using the ELISA （enzyme-linked immunosorbent assay） technique. Out of 180 sero-samples, 46 （25.55%） were positive. Females of the age range 18-35 years had the highest rate of sero-positive samples 14 （38.88%）, while the highest percentage of sero-negative samples was found in males of the age range 50-80 years. The other sero-positive samples were： 6 （26.08%）, 6 （25%） and 3 （11.53%） in males of ages between 18-35, 35-50 and 50-80 years, respectively, and 11 （29.72%） and 6 （17.64%） in females in the age ranges 35-50 and 50-80 years, respectively. The mean concentration of Anti- B. burgdorferi antibody was higher （16.7 U/mL） when compared with mean concentration of normal value （5.5 U/mL）, P 〈 0.001.
