Several mesoporous TiO2 （MT） materials were synthesized under different conditions following a hydrothermal procedure using poly（ethylene-glycol）- block-poly（propylene-glycol）-block-poly（ethylene-glycol） （P12...Several mesoporous TiO2 （MT） materials were synthesized under different conditions following a hydrothermal procedure using poly（ethylene-glycol）- block-poly（propylene-glycol）-block-poly（ethylene-glycol） （P123） as the template and titanium isopropoxide as the titanium source. The molar ratios of Ti/P123, and the pH values of the reaction solution in an autoclave were investigated. Various techniques such as Brunauer-Emmett-Teller （BET）, X-ray diffraction （XRD）, X-ray photoelectron spectroscopy （XPS）, laser Raman spectrometry （LRS）, scanning electron microscopy （SEM）, and high-resolution transmission electron microscopy （HRTEM） were used to characterize the products. Then, these materials were assembled into dye-sensitized solar cells （DSSCs）. Analysis of the J-V curves and electrochemical impedance spectroscopy （EIS） were applied to characterize the cells. The results indicated that the specific surface area and crystalline structure of these materials provide the possibility of high photocurrent for the cells, and that the structural characteristics of the specimens led to increased electron transfer resistance of the cells, which was beneficial for the improvement of the photovoltage of the DSSCs. The highest photoelectric conversion efficiency of the cells involving MT materials reached 8.33%, which, compared with that of P25- based solar cell （5.88%）, increased by 41.7%.展开更多
Protein phosphorylation as one of the most important post-translational modifications in mammalian cells regulates numerous biological processes. Here we propose a novel strategy for the selective isolation and sensit...Protein phosphorylation as one of the most important post-translational modifications in mammalian cells regulates numerous biological processes. Here we propose a novel strategy for the selective isolation and sensitive analysis of mul- ti-phosphopeptides based on TiO2-gratfed mesoporous materials, in which MCM-41 and SBA-15 were chosen as the hard templates. The commercialized IMAC and TiO2 nanopartices were further investigated in the phosphopeptide analysis for comparison. The enrichment efficiency was evaluated and measured by MALDI-TOF mass spectrometry. The results indicated that both TiO2-SBA-15 and TiO2-MCM-41 exhibited the preferential affinity to multi-phosphopeptides compared with the other two widely used strategies. The mesoporous TiO2 based protocol showed highly selective and sensitive properties, where phosphopepddes could be identified at femtomole.展开更多
文摘Several mesoporous TiO2 （MT） materials were synthesized under different conditions following a hydrothermal procedure using poly（ethylene-glycol）- block-poly（propylene-glycol）-block-poly（ethylene-glycol） （P123） as the template and titanium isopropoxide as the titanium source. The molar ratios of Ti/P123, and the pH values of the reaction solution in an autoclave were investigated. Various techniques such as Brunauer-Emmett-Teller （BET）, X-ray diffraction （XRD）, X-ray photoelectron spectroscopy （XPS）, laser Raman spectrometry （LRS）, scanning electron microscopy （SEM）, and high-resolution transmission electron microscopy （HRTEM） were used to characterize the products. Then, these materials were assembled into dye-sensitized solar cells （DSSCs）. Analysis of the J-V curves and electrochemical impedance spectroscopy （EIS） were applied to characterize the cells. The results indicated that the specific surface area and crystalline structure of these materials provide the possibility of high photocurrent for the cells, and that the structural characteristics of the specimens led to increased electron transfer resistance of the cells, which was beneficial for the improvement of the photovoltage of the DSSCs. The highest photoelectric conversion efficiency of the cells involving MT materials reached 8.33%, which, compared with that of P25- based solar cell （5.88%）, increased by 41.7%.
基金supported by the National Basic Research Program of Chi-na (2007CB714506)the National Natural Science Foundation of China (20735005 & 20925517)+1 种基金Science and Technology Committee of Shanghai (10XD1406000 & 09JC1402600)Shanghai Leading Academic Disci-pline B109
文摘Protein phosphorylation as one of the most important post-translational modifications in mammalian cells regulates numerous biological processes. Here we propose a novel strategy for the selective isolation and sensitive analysis of mul- ti-phosphopeptides based on TiO2-gratfed mesoporous materials, in which MCM-41 and SBA-15 were chosen as the hard templates. The commercialized IMAC and TiO2 nanopartices were further investigated in the phosphopeptide analysis for comparison. The enrichment efficiency was evaluated and measured by MALDI-TOF mass spectrometry. The results indicated that both TiO2-SBA-15 and TiO2-MCM-41 exhibited the preferential affinity to multi-phosphopeptides compared with the other two widely used strategies. The mesoporous TiO2 based protocol showed highly selective and sensitive properties, where phosphopepddes could be identified at femtomole.
