Objective: Chemokines are small soluble molecules which mediate leukocyte migration and may be involved in the pathophysiology of preterm labor. We aimed to determine if serum concentrations of selected chemokines are...Objective: Chemokines are small soluble molecules which mediate leukocyte migration and may be involved in the pathophysiology of preterm labor. We aimed to determine if serum concentrations of selected chemokines are changed in preterm labor and delivery. Study design: A novel array-based enzyme-linked immunosorbent assay was used to quantitate serum levels of nine chemokines from a single sample: MDC/CCL22, TARC/CCL17, ITAC/CXCL11, I-309/CCL1, IP-10/CXCL10, MIP-1α/CCL3,-1β/CCL4,-3α/CCL20 and-3β/CCL19. Women in preterm labor who delivered (n = 17), women at preterm pregnancy not in labor (n = 13) and women in labor at term (n = 8) participated. Results: In the preterm delivery group of patients, the MIP-3β/CCL19 concentration was in mean (±SD) 70.4 ±31.7 pg/mL, which was signifi cantly lower than that in preterm gravidas not in labor of 123 ±34 pg/mL (p < 0.001) and those in labor at term of 118 ±25.6 pg/mL (p < 0.01). The other measured chemokines did not differ significantly. Conclusions: Of a small number of examined chemokines, we were able to show that one of them, MIP-3β/CCL19 was significantly lower in women with preterm labor and delivery. Whether or not this chemokine has a potential as biochemical marker of preterm delivery remains to be determined.展开更多
The selectin family of vascular cell adhesion molecules is comprisedof structurally related carbohydrate binding proteins, which mediate the initial rolling of leukocytes on the activated vascular endothelium. Because...The selectin family of vascular cell adhesion molecules is comprisedof structurally related carbohydrate binding proteins, which mediate the initial rolling of leukocytes on the activated vascular endothelium. Because this process is one of the crucial events in initiating and maintaining inflammation, selectins are proposed to be an attractive target for the development of new antiinflammatory therapeutics. Here, we demonstrate that the synthetic pan- selectin antagonist bimosiamose is effective in pre- clinical models of psoriasis as well as in psoriatic patients. In vitro bimosiamose proved to be inhibitory to E- or P- selectin dependent lymphocyte adhesion under flow conditions. Using xenogeneic transplantation models, bimosiamose reduced disease severity as well as development of psoriatic plaques in symptomless psoriatic skin. The administration of bimosiamose in patients suffering from psoriasis resulted in a reduction of epidermal thickness and lymphocyte infiltration. The clinical improvementwas statistically significant (P= 0.02) as analyzed by comparison of psoriasis area and severity index before and after treatment. Assessment of safety parameters showed no abnormal findings. These data suggest that pan- selectin antagonismmay be a promising strategy for the treatment of psoriasis and other inflammatory diseases.展开更多
文摘Objective: Chemokines are small soluble molecules which mediate leukocyte migration and may be involved in the pathophysiology of preterm labor. We aimed to determine if serum concentrations of selected chemokines are changed in preterm labor and delivery. Study design: A novel array-based enzyme-linked immunosorbent assay was used to quantitate serum levels of nine chemokines from a single sample: MDC/CCL22, TARC/CCL17, ITAC/CXCL11, I-309/CCL1, IP-10/CXCL10, MIP-1α/CCL3,-1β/CCL4,-3α/CCL20 and-3β/CCL19. Women in preterm labor who delivered (n = 17), women at preterm pregnancy not in labor (n = 13) and women in labor at term (n = 8) participated. Results: In the preterm delivery group of patients, the MIP-3β/CCL19 concentration was in mean (±SD) 70.4 ±31.7 pg/mL, which was signifi cantly lower than that in preterm gravidas not in labor of 123 ±34 pg/mL (p < 0.001) and those in labor at term of 118 ±25.6 pg/mL (p < 0.01). The other measured chemokines did not differ significantly. Conclusions: Of a small number of examined chemokines, we were able to show that one of them, MIP-3β/CCL19 was significantly lower in women with preterm labor and delivery. Whether or not this chemokine has a potential as biochemical marker of preterm delivery remains to be determined.
文摘The selectin family of vascular cell adhesion molecules is comprisedof structurally related carbohydrate binding proteins, which mediate the initial rolling of leukocytes on the activated vascular endothelium. Because this process is one of the crucial events in initiating and maintaining inflammation, selectins are proposed to be an attractive target for the development of new antiinflammatory therapeutics. Here, we demonstrate that the synthetic pan- selectin antagonist bimosiamose is effective in pre- clinical models of psoriasis as well as in psoriatic patients. In vitro bimosiamose proved to be inhibitory to E- or P- selectin dependent lymphocyte adhesion under flow conditions. Using xenogeneic transplantation models, bimosiamose reduced disease severity as well as development of psoriatic plaques in symptomless psoriatic skin. The administration of bimosiamose in patients suffering from psoriasis resulted in a reduction of epidermal thickness and lymphocyte infiltration. The clinical improvementwas statistically significant (P= 0.02) as analyzed by comparison of psoriasis area and severity index before and after treatment. Assessment of safety parameters showed no abnormal findings. These data suggest that pan- selectin antagonismmay be a promising strategy for the treatment of psoriasis and other inflammatory diseases.
