An expressive plasmid pSMTPCH was constructed from porcine growth hormone gene,sheep metallothionein promoter (MT-011)and the vector,pUC19. The linear pSMTPGH and circular pSV2-dhfr were cotransfected into CHO-dhfr ce...An expressive plasmid pSMTPCH was constructed from porcine growth hormone gene,sheep metallothionein promoter (MT-011)and the vector,pUC19. The linear pSMTPGH and circular pSV2-dhfr were cotransfected into CHO-dhfr cell by calcium phosphate coprecipitation. Positive clones made up 74% of total clones, which were identified with ELISA. The expression of pSMTPGH was induced by 0.5 μM of Cd￣++. The clone 1-C-3 was found to secrete hGH at the level of 3800 μg/10￣6 cells/24 hrs in media containing 10 μMTX. After 20 generations in culture, the clone was still stable with hGH expression.The molecular weight of secreted protein was the same as that of the natural pGH, 22KD;the identity was further supported by Western blot.展开更多
By the end of 2015, total length of China＇s long-distance gas pipelines has exceeded 70,O00km. Onshore stratey, ic import paths have been formed, domestic trunk networks perfected and gas storages construction pace a...By the end of 2015, total length of China＇s long-distance gas pipelines has exceeded 70,O00km. Onshore stratey, ic import paths have been formed, domestic trunk networks perfected and gas storages construction pace acceh＇rated. The Chinese section of Russia-China East Gas Pipeline was officially commenced, which marked that the northeast import path entered the stage ofconstruction. There are 13 LNG terminals in China. The National Development and Rerorm Commission （NDRC） approved China＇s.first private-owned LNG terminal- the Zhoushan LNG terminal. In the coming.five years, the focus of institutional reform will be the independence of pipelines and networks, the focus of construction will be regional networks and branch pipelines, and joint ventures will be the mainstream for gas pipeline construction and operation.展开更多
文摘An expressive plasmid pSMTPCH was constructed from porcine growth hormone gene,sheep metallothionein promoter (MT-011)and the vector,pUC19. The linear pSMTPGH and circular pSV2-dhfr were cotransfected into CHO-dhfr cell by calcium phosphate coprecipitation. Positive clones made up 74% of total clones, which were identified with ELISA. The expression of pSMTPGH was induced by 0.5 μM of Cd￣++. The clone 1-C-3 was found to secrete hGH at the level of 3800 μg/10￣6 cells/24 hrs in media containing 10 μMTX. After 20 generations in culture, the clone was still stable with hGH expression.The molecular weight of secreted protein was the same as that of the natural pGH, 22KD;the identity was further supported by Western blot.
文摘By the end of 2015, total length of China＇s long-distance gas pipelines has exceeded 70,O00km. Onshore stratey, ic import paths have been formed, domestic trunk networks perfected and gas storages construction pace acceh＇rated. The Chinese section of Russia-China East Gas Pipeline was officially commenced, which marked that the northeast import path entered the stage ofconstruction. There are 13 LNG terminals in China. The National Development and Rerorm Commission （NDRC） approved China＇s.first private-owned LNG terminal- the Zhoushan LNG terminal. In the coming.five years, the focus of institutional reform will be the independence of pipelines and networks, the focus of construction will be regional networks and branch pipelines, and joint ventures will be the mainstream for gas pipeline construction and operation.
