Elicitor-induced phosphorylation of tyrosine residues in proteins of potato was studied. Proteins of crude extract of suspension culture of potato were analyzed by one- and two-dimensional electrophoresis followed by ...Elicitor-induced phosphorylation of tyrosine residues in proteins of potato was studied. Proteins of crude extract of suspension culture of potato were analyzed by one- and two-dimensional electrophoresis followed by Western blotting with monoclonal antibodies PY20 to phosphotyrosine proteins. One- and two-dimensional electrophoresis revealed l l and 25 tyrosine-phosphorylated proteins, respectively. Glycoprotein increased the phosphorylation level of most of these proteins.展开更多
Military talents are those who have higher culture and scientific quality, much strong innovation consciousness and thinking ability. The overall objective of military talent training is to cultivate the comprehensive...Military talents are those who have higher culture and scientific quality, much strong innovation consciousness and thinking ability. The overall objective of military talent training is to cultivate the comprehensive-quality military personnel that meet the requirements of military modernization and preparations for military struggle. In this paper, we construct the military talent training objectives model, and on this basis, we propose the innovative military talent training mode, from the perspective of curriculum content system and talent quality assurance system.展开更多
In contrast to the toxic inorganic forms of selenium to life at very low concentrations, the organic-selenium compounds are of considerable interest and several of them play essential roles in cell biochemistry and nu...In contrast to the toxic inorganic forms of selenium to life at very low concentrations, the organic-selenium compounds are of considerable interest and several of them play essential roles in cell biochemistry and nutritional science. Se-yeast （Selenium-enriched yeast） is a common form of selenium used to supplement dietary intake of this important trace mineral. In the present study, we tried to prepare an organic selenocystine using locally isolated bread yeast （Saccharomyces cerevisiae）. A novel locally prepared date extract media enriched by addition of 0.2% KH2PO4 （potassium phosphate）, 0.6% ammonium sulfate was adopted as alternative culture media. Differences concentrations of selenium salt （30, 60, 120 and 240 lag/mL） were added to the yeast culture media. While the best concentration of selenium added was 30Bg/mL, it achieved optimal conditions for the growth of yeast and the production of red yeast growth identical to the standard. The products （organic selenocystine） were analyzed by HPLC （High Performance Liquid Chromatography） and AAS （Atomic Absorption Spectrophotometer） comparing with authentic standard obtained from Sigma. Results confirmed the formation of similar selenocystine products.展开更多
Integrating land use type and other geographic information within spatial interpolation has been proposed as a solution to improve the performance and accuracy of soil nutrient mapping at the regional scale. This stud...Integrating land use type and other geographic information within spatial interpolation has been proposed as a solution to improve the performance and accuracy of soil nutrient mapping at the regional scale. This study developed a non-algorithm approach, i.e., applying inverse distance weighting （IDW） and ordinary kriging （OK）, to individual land use types rather than to the whole watershed, to determine if this improved the performance in mapping soil total C （TC）, total N （TN）, and total P （TP） in a 200-km2 urbanizing watershed in Southeast China. Four land use types were identified by visual interpretation as forest land, agricultural land, green land, and urban land. One hundred and fifty soil samples （0-10 cm） were taken according to land use type and patch size. Results showed that the non-algorithm approach, interpolation based on individual land use types, substantially improved the performance of IDW and OK for mapping TC, TN, and TP in the watershed. Root mean square errors were reduced by 3.9% for TC, 10.770 for TN, and 25.9% for TP by the application of IDW, while the improvements by OK were slightly lower as 0.9% for TC, 7.7% for TN, and 18.1% for TP. Interpolations based on individual land use types visually improved depiction of spatial patterns for TC, TN, and TP in the watershed relative to interpolations by the whole watershed. Substantial improvements might be expected with denser sampling points. We suggest that this non-algorithm approach might provide an alternative to algorithm-based approaches to depict watershed-scale nutrient patterns.展开更多
文摘Elicitor-induced phosphorylation of tyrosine residues in proteins of potato was studied. Proteins of crude extract of suspension culture of potato were analyzed by one- and two-dimensional electrophoresis followed by Western blotting with monoclonal antibodies PY20 to phosphotyrosine proteins. One- and two-dimensional electrophoresis revealed l l and 25 tyrosine-phosphorylated proteins, respectively. Glycoprotein increased the phosphorylation level of most of these proteins.
文摘Military talents are those who have higher culture and scientific quality, much strong innovation consciousness and thinking ability. The overall objective of military talent training is to cultivate the comprehensive-quality military personnel that meet the requirements of military modernization and preparations for military struggle. In this paper, we construct the military talent training objectives model, and on this basis, we propose the innovative military talent training mode, from the perspective of curriculum content system and talent quality assurance system.
文摘In contrast to the toxic inorganic forms of selenium to life at very low concentrations, the organic-selenium compounds are of considerable interest and several of them play essential roles in cell biochemistry and nutritional science. Se-yeast （Selenium-enriched yeast） is a common form of selenium used to supplement dietary intake of this important trace mineral. In the present study, we tried to prepare an organic selenocystine using locally isolated bread yeast （Saccharomyces cerevisiae）. A novel locally prepared date extract media enriched by addition of 0.2% KH2PO4 （potassium phosphate）, 0.6% ammonium sulfate was adopted as alternative culture media. Differences concentrations of selenium salt （30, 60, 120 and 240 lag/mL） were added to the yeast culture media. While the best concentration of selenium added was 30Bg/mL, it achieved optimal conditions for the growth of yeast and the production of red yeast growth identical to the standard. The products （organic selenocystine） were analyzed by HPLC （High Performance Liquid Chromatography） and AAS （Atomic Absorption Spectrophotometer） comparing with authentic standard obtained from Sigma. Results confirmed the formation of similar selenocystine products.
基金supported by the Knowledge Innovation Program of Chinese Academy of Sciences(No.KZCX2-YWJC402)the Hundred Talents Program of Chinese Academy of Sciences(No.A0815)+1 种基金the National Natural Science Foundation of China(No.41371474)supported by the Chinese Academy of Sciences Visiting Professorships for Senior International Scientists in 2011(No.2011T2Z18)
文摘Integrating land use type and other geographic information within spatial interpolation has been proposed as a solution to improve the performance and accuracy of soil nutrient mapping at the regional scale. This study developed a non-algorithm approach, i.e., applying inverse distance weighting （IDW） and ordinary kriging （OK）, to individual land use types rather than to the whole watershed, to determine if this improved the performance in mapping soil total C （TC）, total N （TN）, and total P （TP） in a 200-km2 urbanizing watershed in Southeast China. Four land use types were identified by visual interpretation as forest land, agricultural land, green land, and urban land. One hundred and fifty soil samples （0-10 cm） were taken according to land use type and patch size. Results showed that the non-algorithm approach, interpolation based on individual land use types, substantially improved the performance of IDW and OK for mapping TC, TN, and TP in the watershed. Root mean square errors were reduced by 3.9% for TC, 10.770 for TN, and 25.9% for TP by the application of IDW, while the improvements by OK were slightly lower as 0.9% for TC, 7.7% for TN, and 18.1% for TP. Interpolations based on individual land use types visually improved depiction of spatial patterns for TC, TN, and TP in the watershed relative to interpolations by the whole watershed. Substantial improvements might be expected with denser sampling points. We suggest that this non-algorithm approach might provide an alternative to algorithm-based approaches to depict watershed-scale nutrient patterns.
