钠-葡萄糖共转运蛋白2抑制剂缓解代谢压力

由于能量摄入过剩促使2型糖尿病患病率迅速增长。新型降糖药物钠．葡萄糖共转运蛋白2（SGLT-2）抑制剂通过抑制肾脏近曲小管的葡萄糖重吸收，直接排出过剩的能量，从而在整体水平上缓解这种能量过剩所产生的代谢压力。代谢压力的缓解进一步改善血糖、血脂、血压，改善胰岛素抵抗与胰岛B细胞功能，从而使心血管与肾脏获益。

Effect of captopril on myocardial energy metabolism in chronic pressure overload rats

Objective To investigate the effects of captopril on cardiac function and levels of energy-rich phosphates in pressure overload induced left ventricular hypertrophy rats. Methods One hundred and twenty SD rats were randomly divided into three groups： sham operation group （SH group, n=40）,coarctation of abdominal aorta group （CAA group, n=40） and captopril treatment lmg~ 100g1 ~ d-1） group （CAP group, n=40）. Left ventricular end-diastolic pressure （LVEDP）, left venh-icular mass index （LVMI）, levels of energy-rich phosphates and morphological changes of the myocardial mitochondria were compared at the 62 and 82 week after operation. Results At 62 week, in CAA group, LVMI and LVEDP were increased and _ dp/dtmax was decreased, while ATP and ADP were decreased and AMP was increased （P〈0.01）. These changes were much obvious at 8th week （P〈0.01）. Compared with those of CAA group, the parameters of heart function and energy-rich phosphates （ATP, ADP, AMP, TAN） in CAP group were improved significantly（P〈0.01） at the 6th and 8th week. In CAP group, the parameters of heart function and energy-rich phosphates （ADP, AMP, TAN） were much better at 8~ week than those at 6th week. The morphological change of mitochondria was less in CAP group than that in CAA group. Conclusion Captopril significantly improves myocardial energy metabolism in pressure overload rats and protects the function of myocardial mitochondria

Effects of Plant Extracts with Antioxidant Activities on Male Mule Ducks＇ Performance

Plant extracts with antioxidant activities have effect on male mule ducks performance, and livestock animals have a strong metabolism activity, due to their high production requirements. It can lead to oxidative stress disorders; deteriorate growth performance and even their health. The objective of this study was to assess the effect of a mixture of active ingredients of plant extracts, known for their antioxidant and anti-inflammatory properties, on performances of mule ducks, in rearing and force-feeding period. Then 3,200 day-old mule ducks （MMGxPKL） were separated in 2 groups （negative control and treatment = EL） ×4 replicates per group for the rearing period. Each group received the same feeding program. EL group was supplemented, until the force-feeding period, with a mixture of actives from plants incorporated at 0.5% in the complete feed. At 84 days, the live weight and the feed consumption were measured for each treatment. Blood samples were also taken to measure the activity of two antioxidant enzymes： SOD （superoxide dismutase） and GPx （glutathione peroxidase）. For force-feeding, the ducks were separated among 3 farmers during 12 days. At slaughter, individual measures of breast and liver were conducted. At 84 days, mean live weight for EL group was improved by 3.7% （p 〈 0.001）. Feed conversion ratio was also improved （4.50 versus 4.62）. At slaughter, breast weight was higher for EL group （p 〈 0.01）. A high variability in the results of the activities of antioxidant enzymes was obtained. A decrease in SOD activity in erythrocytes was observed for the EL group （p 〈 0.05）. The results showed that the actives assessed in this study, distributed to ducks during the rearing period, had a beneficial effect on growth performance over that period. The mechanisms of endogenous antioxidant system and inflammatory responses remain to be elucidated.

Inherited disturbances of phenylalanine metabolic kinetics in essential hypert ension

Objective To clarify whether the disturbances in metabolic kinetics of the essential aminoacid, phenylalanine (phe), are implicated in the genetic pathogenesis of essential hypertension (EH).Methods 1. L-(2, 3D3)-leucine, L-(2, 3D3)-isoleucine, L-15N-lysine, L-(2, 3D3)-valine and L-(2, 3D3)-phe were used for simultaneously studying comparative metabolic kinetics using stable isotope tracer methods with a GC-MS system. Study groups were the offspring with both parents suffering EH (n=10, FH+), 2 or more than 2 parents and grand-parents with EH and stroke (n=12, FS+) and those without genetic predisposition of EH and stroke (n=12, F) groups. 2. By comparing the radioactive counts of ［3H］-phe, and their weight transformation in blood after 1.5?Ci/kg i.v. administration at defined intervals and in tissues obtained after being sacrified among spontaneously hypertensive rats (SHR), 2 kidney-1 clip hypertensive rats (2K1C) and their normotensive controls (WKY). 3. The time transport and concentration transport of ［3H］-L-phe in cpm between the cultured vascular smooth muscle cell of 5th generation in SHR and WKY were compared.Results A single and unique disturbance of metabolic kinetics in phe were found in FH+, FS+ and SHR. The plasma pool or apparent volume of distribution was enlarged, and the turnover rate constants between plasma and cell tended to show a decrease. The pharmacokinetics of phe in 2K1C was not changed. Only phe content in heart and aorta, the vital organs for predicting BP, were higher in SHR than in WKY tissues studied. Both the time and concentration transport were higher in SHR, e.g., an increment in the net-uptake of L-phe by vascular tissue.Conclusion A unique aberrant of metabolic kinetics of phe might be implicated in the inherited pathogenesis of EH and stroke both from clinical and animal studies.

Functional impact of microRNA regulation n models of extreme stress adaptation

When confronted with severe environmental stress, some animals are able to undergo a substantial reorganization of their cellular environment that enables long-term survival. One molecular mechanism of adaptation that has received considerable attention in recent years has been the action of reversible transcriptome regulation by microRNA. The implementation of new computational and high-throughput experimental approaches has started to uncover the vital contributions of microRNA towards stress adaptation. Indeed, recent studies have suggested that microRNA may have a major regulatory influence over a number of cellular processes that are essential to prolonged environmental stress survival. To date, a number of studies have highlighted the role of microRNA in the regulation of a metabolically depressed state, documenting stress-responsive microRNA expression during mammalian hibernation, frog and insect freeze tolerance, and turtle and marine snail anoxia tolerance. These studies collectively indicate a conserved principle of microRNA stress response across phylogeny. As we are on the verge of dissecting the role of microRNA in environmental stress adaptation, this review summarizes recent research advances and the hallmark expression patterns that facilitate stress survival.

Analysis of emergy-based metabolic efficiency and environmental pressure on the local coupling and telecoupling between urbanization and the eco-environment in the Beijing-Tianjin-Hebei urban agglomeration

We use emergy-based urban metabolism analysis in this study to investigate the local coupling and telecoupling between urbanization and the eco-environment in the Beijing-Tianjin-Hebei urban agglomeration. Our analysis encompasses the last 35 years spanning the period between 1980 and 2014. In addition, we analyze urban metabolic efficiency and environmental pressure in these couplings. There are three main conclusions of this study. First, we show that the total metabolic emergy value of the Beijing-Tianjin-Hebei urban agglomeration has increased over the last 35 years, and that external elements have replaced internal ones as the leading contributors to urban metabolism in this region. The proportion of metabolic emergy derived from external elements increased from 30.87% in 1980 to 67.43% in 2014. The emergy extroversion ratio has continued to increase over our study period, while the development of this urban agglomeration had become progressively and more heavily reliant on external elements. Second, over the study period, the metabolic emergy intensity of elements of local coupling and telecoupling in the Beijing-Tianjin-Hebei urban agglomeration has declined at an accelerating rate, from 1.15×10^(21) Sej/100 million RMB in1980 to 9.69×10^(19) Sej/100 million RMB in 2014. This reflects a continuous increase in the economic efficiency of this area.Correspondingly, emergy use per capita increased from 7.8×10^(15) Sej/person in 1980 to 5.83×10^(16) Sej/person in 2014, suggesting a gradual rise in the level of social welfare in this urban agglomeration. However, we also observed a consistently elevated environmental loading ratio(ELR) in our analysis in terms of metabolism of local coupling and telecoupling elements. This result indicates that environmental pressure has also increased constantly within the Beijing-Tianjin-Hebei urban agglomeration. Third,our analysis shows that the ELR of metabolic emergy from internal elements increased from 8.30 in 1980 to 43.46 in 2014, while the ratio from external elements increased from 4.15 to 92.03. Thus, we quantitatively investigated the dependency of development within this urban agglomeration on external elements as well as the resultant environmental load. The conclusions of this study provide the basis for quantitative policy-making in the Beijing-Tianjin-Hebei region, optimizing economic structures, improving economic efficiency, controlling environmental pollution, and promoting the coordinated development of this region.

The sulfur metabolism regulator MetR is a global regulator controlling phytochrome-dependent light responses in Aspergillus nidulans

Phytochrome-dependent light signaling has been studied in several fungi.In Aspergillus nidulans lightstimulated phytochrome activates the high-osmolarity glycerol(HOG)signaling pathway and thereby controls the expression of a large number of genes,many of which are related to stress responses.In a genome-wide expression analysis in A.nidulans we found that phytochrome,fph A,is under strict expression control of the central regulator of the sulfur-starvation response,Met R.This transcriptional regulator is required for the expression of genes involved in inorganic sulfur assimilation.In the presence of organic sulfur,Met R is probably ubiquitinated and possibly degraded and the transcription of sulfur-assimilation genes,e.g.,sulfate permease,is turned off.The expression analysis described here revealed,however,that Met R additionally controls the expression of hundreds of genes,many of which are required for secondary metabolite production.We also show that met R mutation phenocopies fph A deletion,and five other histidine-hybrid kinases are down-regulated in the met R1 mutant.Furthermore,we found that light and phytochrome regulate the expression of at least three carbon–sulfur hydrolases.This work is a further step towards understanding the interplay between light sensing and metabolic pathways.