花生是重要的油料和经济作物,花生种皮色泽存在较大差异,具有白色、红色、紫色、粉色及花斑类型,花斑种皮花生是其中的独特成员。有关花斑花生种皮花青素合成的分子机制存在深入研究的必要性。本研究以花斑种皮花生VG-02为研究材料,采...花生是重要的油料和经济作物,花生种皮色泽存在较大差异,具有白色、红色、紫色、粉色及花斑类型,花斑种皮花生是其中的独特成员。有关花斑花生种皮花青素合成的分子机制存在深入研究的必要性。本研究以花斑种皮花生VG-02为研究材料,采用液相色谱串联质谱(LC-MS/MS)法检测不同发育阶段种皮中花青素的相对含量变化,共检测到12种与种皮颜色相关的代谢物质。在花生种皮着色区(F)与非着色区(B)开花下针(DAF)45 d的F2-B2比较组中差异代谢产物最多,结果表明矢车菊素3-O-半乳糖苷和矢车菊素O-丁香酸含量着色区低于非着色区,差异倍数分别为0.63和2.35;松香花青素O-己糖苷,原花青素A1、A2、B2、B3,矢车菊素,花翠素,花翠素3-O-葡萄糖苷,矢车菊素3-O-半乳糖苷含量着色区高于非着色区,差异倍数1.05~11.55。花翠素和矢车菊素是导致着色区与非着色区颜色差异的主要代谢物。RNA-seq分析表明,1050个差异基因中筛选出与花斑种皮颜色形成高度相关的差异表达基因共27个,包括3个PAL、1个C4H、2个CHS、1个F3H、1个F3′H、2个DFR、2个LAR、2个IAA、4个bHLH和9个MYB,其中上调13个、下调14个。KEGG(Kyoto Encyclopedia of Genes and Genomes)分析表明,与种皮颜色合成相关所富集的代谢通路有苯丙氨酸代谢、苯丙醇生物合成、黄酮和黄酮醇生物合成、类黄酮生物合成、植物激素信号转导以及昼夜节律植物,其中类黄酮生物合成代谢途径是花生种皮花斑形成最直接的代谢途径。对20个差异基因进行qPCR验证,结果表明差异基因qPCR表达趋势与转录组测序结果显著一致。本研究结果对进一步揭示花生花斑种皮花青素合成调控机制具有一定的参考意义。展开更多
基于同位素标记相对和绝对定量(isobaric tags for relative and absolute quantity,iTRAQ)技术,以采自宁夏中宁的“宁杞1号”枸杞果实为样品,以采自新疆精河、内蒙古乌拉特前旗、甘肃瓜州和青海德令哈的“宁杞1号”枸杞果实为对照,进...基于同位素标记相对和绝对定量(isobaric tags for relative and absolute quantity,iTRAQ)技术,以采自宁夏中宁的“宁杞1号”枸杞果实为样品,以采自新疆精河、内蒙古乌拉特前旗、甘肃瓜州和青海德令哈的“宁杞1号”枸杞果实为对照,进行差异蛋白组学的生物信息学分析。结果表明,新疆/宁夏组、内蒙古/宁夏组、甘肃/宁夏组和青海/宁夏组分别有446,166,966个和1015个差异表达蛋白(DEPs),与枸杞多糖合成代谢相关的DEPs共160个。进一步筛选得到23个与枸杞多糖相关DEPs,包括蔗糖磷酸合成酶、β-葡萄糖苷酶和α-半乳糖苷酶等。推测采自不同产地的枸杞果实的多糖合成代谢差异通路主要包括蔗糖、纤维素、半乳聚糖、半纤维素和果胶合成代谢过程,且这些过程均可通过UDP-葡萄糖链接在一起。通过蛋白组学分析枸杞多糖合成途径上的相关蛋白,为深入研究不同产地同种枸杞多糖调控机制提供理论参考。展开更多
Bacillus subtilis produces many chemlcally-dwerse seconaary metaDolltes or interest to chemists ano biologlsts. Based on this, this review gives a detailed overview of the natural components produced by B. subtilis in...Bacillus subtilis produces many chemlcally-dwerse seconaary metaDolltes or interest to chemists ano biologlsts. Based on this, this review gives a detailed overview of the natural components produced by B. subtilis including cyclic lipopeptides, polypeptides, proteins （enzymes）, and non-peptide products. Their structures, bioactive ac- tivities and the relevant variants as novel lead structures for drug discovery are also described. The challenging effects of fermentation metabolites, isolation and purification, as well as the overproduction of bioactive com- pounds from B. subtilis by metabolic engineering, ＇~ere also highlighted. Systematically exploring biosynthetic routes and the functions of secondary metabolites from 13. subtilis may not only be beneficial in improving yields of the products, but also in helping them to be used in food industry and public medical service on a large-scale.展开更多
We compared the cDNA sequences and amino acid sequences of Chalcone synthase （CHS）, Chalcone isomerase （CH1）, and lsoflavone synthase （IFS） in isoflavone biosynthesis pathway, found that their similarity were no...We compared the cDNA sequences and amino acid sequences of Chalcone synthase （CHS）, Chalcone isomerase （CH1）, and lsoflavone synthase （IFS） in isoflavone biosynthesis pathway, found that their similarity were not always consistent; then we compared the amino acid sequences of CHS, CHI, and IFS, found that the clustering relationship of CHS and CHI were different in 13 kind of species; CHS and IFS were the same or similar in leguminous plants Medicago sativa, Medicago truncatula, Pisum sativum, Pueraria Montana, and Glycine max however disaccording with CHI. We prefer the amino acid sequence for cDNA sequence for evolution analysis, and think that the secondary metabolism mostly attributing to environmental stresses, and the functional genes on secondary metabolism pathway are unsuitable for taxonomy analysis.展开更多
文摘花生是重要的油料和经济作物,花生种皮色泽存在较大差异,具有白色、红色、紫色、粉色及花斑类型,花斑种皮花生是其中的独特成员。有关花斑花生种皮花青素合成的分子机制存在深入研究的必要性。本研究以花斑种皮花生VG-02为研究材料,采用液相色谱串联质谱(LC-MS/MS)法检测不同发育阶段种皮中花青素的相对含量变化,共检测到12种与种皮颜色相关的代谢物质。在花生种皮着色区(F)与非着色区(B)开花下针(DAF)45 d的F2-B2比较组中差异代谢产物最多,结果表明矢车菊素3-O-半乳糖苷和矢车菊素O-丁香酸含量着色区低于非着色区,差异倍数分别为0.63和2.35;松香花青素O-己糖苷,原花青素A1、A2、B2、B3,矢车菊素,花翠素,花翠素3-O-葡萄糖苷,矢车菊素3-O-半乳糖苷含量着色区高于非着色区,差异倍数1.05~11.55。花翠素和矢车菊素是导致着色区与非着色区颜色差异的主要代谢物。RNA-seq分析表明,1050个差异基因中筛选出与花斑种皮颜色形成高度相关的差异表达基因共27个,包括3个PAL、1个C4H、2个CHS、1个F3H、1个F3′H、2个DFR、2个LAR、2个IAA、4个bHLH和9个MYB,其中上调13个、下调14个。KEGG(Kyoto Encyclopedia of Genes and Genomes)分析表明,与种皮颜色合成相关所富集的代谢通路有苯丙氨酸代谢、苯丙醇生物合成、黄酮和黄酮醇生物合成、类黄酮生物合成、植物激素信号转导以及昼夜节律植物,其中类黄酮生物合成代谢途径是花生种皮花斑形成最直接的代谢途径。对20个差异基因进行qPCR验证,结果表明差异基因qPCR表达趋势与转录组测序结果显著一致。本研究结果对进一步揭示花生花斑种皮花青素合成调控机制具有一定的参考意义。
文摘基于同位素标记相对和绝对定量(isobaric tags for relative and absolute quantity,iTRAQ)技术,以采自宁夏中宁的“宁杞1号”枸杞果实为样品,以采自新疆精河、内蒙古乌拉特前旗、甘肃瓜州和青海德令哈的“宁杞1号”枸杞果实为对照,进行差异蛋白组学的生物信息学分析。结果表明,新疆/宁夏组、内蒙古/宁夏组、甘肃/宁夏组和青海/宁夏组分别有446,166,966个和1015个差异表达蛋白(DEPs),与枸杞多糖合成代谢相关的DEPs共160个。进一步筛选得到23个与枸杞多糖相关DEPs,包括蔗糖磷酸合成酶、β-葡萄糖苷酶和α-半乳糖苷酶等。推测采自不同产地的枸杞果实的多糖合成代谢差异通路主要包括蔗糖、纤维素、半乳聚糖、半纤维素和果胶合成代谢过程,且这些过程均可通过UDP-葡萄糖链接在一起。通过蛋白组学分析枸杞多糖合成途径上的相关蛋白,为深入研究不同产地同种枸杞多糖调控机制提供理论参考。
基金Supported by the National Natural Science Foundation of China(21376215)the National Science and Technology Major Project of New Drug,China(2012ZX09103101-075)+2 种基金the Innovative Research Platform co-constructed by Zhejiang University and Taizhou City,and the Science and Technology Project of Zhejiang Province(2014C33174)the Major State Basic Research Development Program of China(2011CB710803)the National High-Tech Research and Development Program of China(2012AA022302)
文摘Bacillus subtilis produces many chemlcally-dwerse seconaary metaDolltes or interest to chemists ano biologlsts. Based on this, this review gives a detailed overview of the natural components produced by B. subtilis including cyclic lipopeptides, polypeptides, proteins （enzymes）, and non-peptide products. Their structures, bioactive ac- tivities and the relevant variants as novel lead structures for drug discovery are also described. The challenging effects of fermentation metabolites, isolation and purification, as well as the overproduction of bioactive com- pounds from B. subtilis by metabolic engineering, ＇~ere also highlighted. Systematically exploring biosynthetic routes and the functions of secondary metabolites from 13. subtilis may not only be beneficial in improving yields of the products, but also in helping them to be used in food industry and public medical service on a large-scale.
文摘We compared the cDNA sequences and amino acid sequences of Chalcone synthase （CHS）, Chalcone isomerase （CH1）, and lsoflavone synthase （IFS） in isoflavone biosynthesis pathway, found that their similarity were not always consistent; then we compared the amino acid sequences of CHS, CHI, and IFS, found that the clustering relationship of CHS and CHI were different in 13 kind of species; CHS and IFS were the same or similar in leguminous plants Medicago sativa, Medicago truncatula, Pisum sativum, Pueraria Montana, and Glycine max however disaccording with CHI. We prefer the amino acid sequence for cDNA sequence for evolution analysis, and think that the secondary metabolism mostly attributing to environmental stresses, and the functional genes on secondary metabolism pathway are unsuitable for taxonomy analysis.
