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人体运动能耗分配关系的实验研究 被引量:4
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作者 石新燕 李美霞 《西安体育学院学报》 CSSCI 北大核心 2012年第4期492-496,共5页
目的估测人体运动过程中参与工作的各子系统的能耗,建立较为精细的人体运动能耗分配关系。方法研究对13名大学生(男性)进行了功率自行车设定负荷下的运动代谢实验、多域多维心功能检测以及动作技术影像拍摄和解析,并对人体运动能量系统... 目的估测人体运动过程中参与工作的各子系统的能耗,建立较为精细的人体运动能耗分配关系。方法研究对13名大学生(男性)进行了功率自行车设定负荷下的运动代谢实验、多域多维心功能检测以及动作技术影像拍摄和解析,并对人体运动能量系统和做功进行了建模分析。结果受试者在给定的相同负荷功率下以不同转速蹬功率自行车时的运动能耗差异显著;总搏功两两之间均无显著性差异(P>0.05);无用功两两之间比较差异显著(P<0.001)。结论心脏做功仅与负荷量(输出功率)有关,而在给定负荷情况下心脏做功与速度大小无关;运动参数的改变显著影响运动无用功,也影响总能量消耗;在中等强度踏蹬自行车运动中,呼吸系统、循环系统、骨骼肌系统分别消耗总能量的11.02%,52.86%,5.36%;剩余30%左右的能量为对外做功和移动肢体做功(分别为25.72%和5.04%)。研究还为估测运动能耗在不同系统间的分配关系提供了一种可行方法。 展开更多
关键词 能耗分配 机械功 代谢测量 中等强度 功率自行车
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Correlation of serum leptin levels with anthropometric and metabolic parameters and biochemical liver function in Chinese patients with chronic hepatitis C virus infection 被引量:4
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作者 Zheng-WenLiu NiZhang +6 位作者 Qun-YingHan Jun-TaoZeng Yong-LieChu Jian-MingQiu Ya-WenWang Lie-TingMa xiao-Qinwang 《World Journal of Gastroenterology》 SCIE CAS CSCD 2005年第22期3357-3362,共6页
AIM: To determine serum leptin levels and investigate their correlations with anthropometric and metabolic parameters and biochemical liver function in patients with chronic hepatitis C virus (HCV) infection and their... AIM: To determine serum leptin levels and investigate their correlations with anthropometric and metabolic parameters and biochemical liver function in patients with chronic hepatitis C virus (HCV) infection and their potential clinical implications.METHODS: Forty-two chronic HCV-infected patients without anti-viral treatment were enrolled in this study, 30 patients had chronic hepatitis C, 10 had cirrhosis, and 2 had hepatocellular carcinoma (HCC). Thirty age- and sex-matched healthy individuals served as controls. Serum leptin levels were determined by ELISA. The biochemical liver function and serum lipids were determined at the same time. The height and body weight of patients and controls were measured, and body mass index (BMI) and body fat were calculated simultaneously. The correlations of serum leptin levels with anthropometric and metabolic parameters and biochemical liver function were assessed statistically.RESULTS: The mean of serum leptin levels in patients with chronic hepatitis C, HCV-associated cirrhosis, HCVassociated HCC and control groups was (6.13±3.94),(5.25±4.21), (4.17±0.28), and (3.59±3.44) ng/mL, respectively. The serum leptin level in patients with chronic hepatitis C was significantly higher than that in controls. The serum leptin levels between cirrhotic patients and controls and between male and female cirrhotic patients had no significant difference. Serum leptin levels were positively-correlated with body fat, BMI, and apolipoprotein B (Apo B) in patients with chronic HCV infection. The serum alanine aminotransferase (ALT) levels were closelycorrelated with BMI in patients with chronic hepatitis C. CONCLUSION: HCV infection interferes with fat and lipid metabolism in patients with chronic HCV infection and leptin may play a role in hepatosteatosis. 展开更多
关键词 Chronic hepatitis C LEPTIN Anthropometric parameter Lipid metabolism
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RNA-seq reveals the critical role of Csp A in regulating Brucella melitensis metabolism and virulence 被引量:5
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作者 Zhen Wang Wenxiao Liu +2 位作者 Tonglei Wu Pengfei Bie Qingmin Wu 《Science China(Life Sciences)》 SCIE CAS CSCD 2016年第4期417-424,共8页
Brucella melitensis is a facultative intracellular bacterium that replicates within macrophages. The ability of Brucella to survive and multiply in the hostile environment of host macrophages is essential for its viru... Brucella melitensis is a facultative intracellular bacterium that replicates within macrophages. The ability of Brucella to survive and multiply in the hostile environment of host macrophages is essential for its virulence. The cold shock protein Csp A plays an important role in the virulence of B. melitensis. To analyze the genes regulated by Csp A, the whole transcriptomes of B. melitensis NI?csp A and its parental wild-type strain, B. melitensis NI, were sequenced and analyzed using the Solexa/Illumina sequencing platform. A total of 446 differentially expressed genes were identified, including 324 up-regulated and 122 down-regulated genes. Numerous genes identified are involved in amino acid, fatty acid, nitrogen, and energy metabolism. Interestingly, all genes involved in the type IV secretion system and Lux R-type regulatory protein Vjb R were significantly down-regulated in NI?csp A. In addition, an effector translocation assay confirmed that the function of T4 SS in NI?csp A is influenced by deletion of the csp A gene. These results revealed the differential phenomena associated with virulence and metabolism in NI?csp A and NI, providing important information for understanding detailed Csp A-regulated interaction networks and Brucella pathogenesis. 展开更多
关键词 Brucella melitensis cold shock protein transcriptome virulence
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