Objective: To investigate the anti-proliferation e?ect of oridonin on leukemic NB4 cells and its mechanism. Methods: NB4 cells in culture medium in vitro were given di?erent concentrations of o...Objective: To investigate the anti-proliferation e?ect of oridonin on leukemic NB4 cells and its mechanism. Methods: NB4 cells in culture medium in vitro were given di?erent concentrations of oridonin. The inhibitory rate of the cells were measured by MTT assay, cell apoptotic rate was detected by ?ow cytometry(FCM), morphology of cell apoptosis was observed by hoechst 33258 ?uorescence staining , and the activity of telomerase was detected using TRAP-PCR-ELISA before and after apoptosis occurred. Results: Oridonin (over 8 μmol/L) could decrease the telomerase activity, inhibit the growth of NB4 cells and induce apoptosis signi?cantly in a time- and dose-dependent manner. Marked morphological changes of cell apoptosis were observed by hoechst 33258 ?uorescence staining especially after the cells treated by oridonin for 48–60 h. Conclusion: Oridonin could inhibit the proliferation and induce the apoptosis of NB4 cells in vitro. One of the mechanisms may be the decrease of the telomerase activity of NB4 cells.展开更多
OBJECTIVE:To explore the anti-aging effects of mild-warming moxibustion on Bcl-2 and PKC expression in peripheral blood and general symptoms in elderly people.METHODS:A total of 61 elderly people and 30 non-elderly pe...OBJECTIVE:To explore the anti-aging effects of mild-warming moxibustion on Bcl-2 and PKC expression in peripheral blood and general symptoms in elderly people.METHODS:A total of 61 elderly people and 30 non-elderly people were enrolled.The total effective rate of mild-warming moxibustion was assessed by symptom scores,and Bcl-2 and PKC expression in peripheral blood was detected by flow cytometry.RESULTS:The total effective rate in the mild-warming moxibustion group was significantly higher than in the blank control group(P<0.01).Bcl-2 and PKC expression rates in peripheral blood in the blank control group were lower than in the normal control group(P<0.01),but higher after mild-warming moxibustion(P<0.01).CONCLUSION:The anti-aging effects of mild-warm-ing moxibustion may be due to increased Bcl-2 and PKC expression in peripheral blood in aged people.展开更多
OBJECTIVE： To investigate how the pretreatment of mice with Ganoderma spores affected the apop- tosis of their splenic lymphocytes induced by dexa- methasone after 19 days treatment. METHODS： Sixty Kunming mice were...OBJECTIVE： To investigate how the pretreatment of mice with Ganoderma spores affected the apop- tosis of their splenic lymphocytes induced by dexa- methasone after 19 days treatment. METHODS： Sixty Kunming mice were randomly di- vided into six groups： blank control groupdrenched with normal saline; a drug control group drenched with 150 mg/mL Ganoderma spores; a model group treated with saline; a low dose group with S0 mg/mL Ganoderma spores; a moderate dose group with 100 mg/mL Ganoderma spores; and a high dose group with 150 mg/mL Ganoderma spores. The effect of Ganoderma spores on apoptosis in spleen lymphocytes was analyzed. All groups were treated for 19 days. On day 20, the model group and the 3 treatment groups were intraperitoneally injected dexamethasone to induce apoptosis. Splenic index and apoptosis indes were employed to measure cell apoptosis RESULTS： The results showed that Ganoderma spores reduced the splenic index to different de- grees in each group and the best effect was seen in the high dose group （P〈0.05）. Terminal dexynucleo- tidyl transferase （TdT）-mediated 2＇-Deoxyuridine 5＇-Triphosphate nick end labeling staining revealed that the apoptotic index in all groups administered Ganoderma spores differed significantly from the model group, and a dose-response was observed. Flow cytometric analysis indicated that spleen lym- phocyte apoptosis in the model group was exten- sive. Each dose of Ganoderma spores inhibited dexamethasone-induced apoptosis in spleen lym- phocytes, and a dose-response was observed as well. The highest dose of Ganoderma spores de- creased Malondialdehyde content in serum in- duced by dexamethasone （P〈0.05）. CONCLUSION： The findings imply that the pretreat- ment of the mice with Ganoderma spores could re- duce the apoptosis rate induced by dexametha- sone in their splenic lymphocytes.展开更多
文摘Objective: To investigate the anti-proliferation e?ect of oridonin on leukemic NB4 cells and its mechanism. Methods: NB4 cells in culture medium in vitro were given di?erent concentrations of oridonin. The inhibitory rate of the cells were measured by MTT assay, cell apoptotic rate was detected by ?ow cytometry(FCM), morphology of cell apoptosis was observed by hoechst 33258 ?uorescence staining , and the activity of telomerase was detected using TRAP-PCR-ELISA before and after apoptosis occurred. Results: Oridonin (over 8 μmol/L) could decrease the telomerase activity, inhibit the growth of NB4 cells and induce apoptosis signi?cantly in a time- and dose-dependent manner. Marked morphological changes of cell apoptosis were observed by hoechst 33258 ?uorescence staining especially after the cells treated by oridonin for 48–60 h. Conclusion: Oridonin could inhibit the proliferation and induce the apoptosis of NB4 cells in vitro. One of the mechanisms may be the decrease of the telomerase activity of NB4 cells.
基金Supported by Nanjing University of Chinese Medicine,Key laboratory of Acupuncture Combined with Medication,NO.KJA200914
文摘OBJECTIVE:To explore the anti-aging effects of mild-warming moxibustion on Bcl-2 and PKC expression in peripheral blood and general symptoms in elderly people.METHODS:A total of 61 elderly people and 30 non-elderly people were enrolled.The total effective rate of mild-warming moxibustion was assessed by symptom scores,and Bcl-2 and PKC expression in peripheral blood was detected by flow cytometry.RESULTS:The total effective rate in the mild-warming moxibustion group was significantly higher than in the blank control group(P<0.01).Bcl-2 and PKC expression rates in peripheral blood in the blank control group were lower than in the normal control group(P<0.01),but higher after mild-warming moxibustion(P<0.01).CONCLUSION:The anti-aging effects of mild-warm-ing moxibustion may be due to increased Bcl-2 and PKC expression in peripheral blood in aged people.
基金Supported by the Natural Science Foundation of Fujian Province (No.2013J01069No.2012J01067)
文摘OBJECTIVE： To investigate how the pretreatment of mice with Ganoderma spores affected the apop- tosis of their splenic lymphocytes induced by dexa- methasone after 19 days treatment. METHODS： Sixty Kunming mice were randomly di- vided into six groups： blank control groupdrenched with normal saline; a drug control group drenched with 150 mg/mL Ganoderma spores; a model group treated with saline; a low dose group with S0 mg/mL Ganoderma spores; a moderate dose group with 100 mg/mL Ganoderma spores; and a high dose group with 150 mg/mL Ganoderma spores. The effect of Ganoderma spores on apoptosis in spleen lymphocytes was analyzed. All groups were treated for 19 days. On day 20, the model group and the 3 treatment groups were intraperitoneally injected dexamethasone to induce apoptosis. Splenic index and apoptosis indes were employed to measure cell apoptosis RESULTS： The results showed that Ganoderma spores reduced the splenic index to different de- grees in each group and the best effect was seen in the high dose group （P〈0.05）. Terminal dexynucleo- tidyl transferase （TdT）-mediated 2＇-Deoxyuridine 5＇-Triphosphate nick end labeling staining revealed that the apoptotic index in all groups administered Ganoderma spores differed significantly from the model group, and a dose-response was observed. Flow cytometric analysis indicated that spleen lym- phocyte apoptosis in the model group was exten- sive. Each dose of Ganoderma spores inhibited dexamethasone-induced apoptosis in spleen lym- phocytes, and a dose-response was observed as well. The highest dose of Ganoderma spores de- creased Malondialdehyde content in serum in- duced by dexamethasone （P〈0.05）. CONCLUSION： The findings imply that the pretreat- ment of the mice with Ganoderma spores could re- duce the apoptosis rate induced by dexametha- sone in their splenic lymphocytes.
