[Objective] This study aimed optimize sequence-related amplified polymor- phism （SRAP）-PCR system for Paphiopedilum hirsutissimum. [Method] Using P. hir- sutissimum leaf as the material, a single-factor test was ado...[Objective] This study aimed optimize sequence-related amplified polymor- phism （SRAP）-PCR system for Paphiopedilum hirsutissimum. [Method] Using P. hir- sutissimum leaf as the material, a single-factor test was adopted to optimize the fac- tors in SRAP-PCR system, including the concentrations of dNTPs, Mg2＋, Taq poly- merase, DNA template and primers. [Result] The optimized SRAP-PCR system con- tained 2.5 μl of 10xPCR buffer, 0.15 mmol/L dNTPs, 2.0 mmol/L Mg2＋, 0.3 mmol/L primer each, 0.3 U Taq polymerase and 1 μl of DNA template. [Conclusion] This system can amplify clear and repeatable DNA profiles, which can be applied for further study about P. hirsutissimum.展开更多
[Objective] By using the genomic DNA of Cymbidium faberi Rolfe as template,the factors that affect the result of ISSR-PCR reaction system were researched and the optimal system was established.[Method] The genomic DNA...[Objective] By using the genomic DNA of Cymbidium faberi Rolfe as template,the factors that affect the result of ISSR-PCR reaction system were researched and the optimal system was established.[Method] The genomic DNA was extracted from C.faberi Rolfe with method of modified CTAB.Different factors which affected ISSR amplification reaction were optimized.[Result] High-quality genomic DNA was obtained from C.faberi Rolfe.And the optimal reaction system was as follows：25 μl amplification reactions system contained 2.5 μl 10 × PCR buffer,2.0 mmol/L MgCl2,60 ng template DNA,160 μmol/L dNTPs,1.25 U Taq DNA polymerase,0.4 μmol/L ISSR primer and 15.85 μl ddH2O.The optimal amplification procedures were pre-denaturing for 5 min at 94 ℃,followed by 40 cycles of denaturing for 30 s at 94 ℃,annealing for 30 s at a temperature of 2-3 ℃ lower than melting temperature of each primer pair,extension for 50 s at 72 ℃.Then extension step of 7 min at 72 ℃ was performed.[Conclusion] The optimal system could provide a favorable basis for further study on genetic diversity of C.faberi Rolfe by using ISSR molecular marker technique.展开更多
[Objective] This research aimed to search a best method for extracting the genomic DNA of Cymbidium ensifolium and establish the optimized ISSR-PCR reaction system.[Method] Genomic DNA was extracted from C.ensifolium ...[Objective] This research aimed to search a best method for extracting the genomic DNA of Cymbidium ensifolium and establish the optimized ISSR-PCR reaction system.[Method] Genomic DNA was extracted from C.ensifolium leaves by modified CTAB method.ISSR-PCR reaction system for C.ensifolium was optimized.[Result] High-quality genomic DNA was obtained from C.ensifolium.The 25 μl optimized ISSR-PCR reaction system for C.ensifolium contained 2.5 μl 10× PCR buffer,2.5 mmol/L MgCl2,240 ng template DNA,160 μmol/L dNTPs,1.25 U Taq DNA polymerase,0.4 μmol/L primer and 15.78 μl ddH2O.The optimal PCR procedures were：94 ℃ pre-denaturation for 5 min and then 40 cycles,94 ℃ denaturation for 30 s,50-60 ℃ annealing for 30 s （annealing temperature according to different primers）,72 ℃ extension for 50 s and a 72 ℃ extension for 7 min.[Conclusion] An optimized ISSR-PCR reaction system for C.ensifolium was established,which provides a basis for further study on genetic diversity of C.ensifolium by using ISSR molecular marker technique.展开更多
Geographic concentration of industries is the regional- ized distribution of some industries in certain areas, which focuses on the ratio of a certain industry to the whole industries (He and Liu, 2006). In this paper...Geographic concentration of industries is the regional- ized distribution of some industries in certain areas, which focuses on the ratio of a certain industry to the whole industries (He and Liu, 2006). In this paper we explore the improved M function of geographic concentration that adds the parameter of the number of firms according to the definition of geographic concentration of industries. The spatial distribution of the main manufacturing industries of Lanzhou urban area is evaluated based on it. The results of the evaluation imply that the spatial distribution of the main manufacturing industries is more concentrated than that of others in Lanzhou and it can absorb lots of labor forces. But the incidence, competition ability and density of the distribution of enterprises are different for each single sector, and enterprises with different production features are located closely. And three main problems are discovered. Finally, three countermeasures are put forward: locating the industrial enterprises in urban areas in a proper way through planning and policies; adjusting the industrial structure of the inner city; strengthening the local rearrangement of the existing industrial concentration areas.展开更多
[ Objective] The paper aimed to study the optimum extraction technology of pigments from petals of Matthiola incana（ L. ） R. Br. of differ- ent colors. [Method] Petal pigments of Aida variety （white violet）, Franc...[ Objective] The paper aimed to study the optimum extraction technology of pigments from petals of Matthiola incana（ L. ） R. Br. of differ- ent colors. [Method] Petal pigments of Aida variety （white violet）, Francesco variety （ red violet） and Arabella variety （ purple violet） were ana- lyzed by colorimetric reaction method and ultraviolet-visible spectrum method, and the optimum extraction technology of flavonoid compounds from petals of them was determined by single factor experiment and orthogonal experiment. [ Result] The optimum extraction conditions of white violet were as follows： with 95% ethanol ＋ 5% HCI as solvent, solid-liquid ratio （ 1 ： 40）, extraction time （3 h）, extraction temperature （65 ℃ ）. The opti- mum extraction conditions of red violet and purple violet were as follows： with acetone as solvent, solid-liquid ratio （1：40）, extraction time （3 h）, extraction temperature （65 ℃）. [ Conclusion] The study provided theoretical foundation for further development and exploitation of pigments from M. incana（ L. ） R. Br.展开更多
The paper analyzes five Finnish case companies to study the sourcing process from China. A framework of a six-stage sourcing process is developed that includes finding and choosing suppliers, making contracts and orde...The paper analyzes five Finnish case companies to study the sourcing process from China. A framework of a six-stage sourcing process is developed that includes finding and choosing suppliers, making contracts and orders, performing production and quality control, planning logistics, inspecting purchases, and developing supply chain management. Each stage describes the activities from the channels of seeking suppliers to cooperative areas for further development. The case study illustrates the practices of sourcing from China are actually quite similar to the sourcing process in other countries. The study highlights that quality control is the core issue in sourcing from China. Moreover, the difference in understanding sustainable quality affects supplier screening and quality control. Close cooperation on quality control is the key to managing the supply chain to increase competitive advantage through sourcing from China.展开更多
The paper presents selected experience of the authors resulting from the optimization tests of double-regulated water turbines. Among the methods for measuring the discharge through the turbine used in such tests, par...The paper presents selected experience of the authors resulting from the optimization tests of double-regulated water turbines. Among the methods for measuring the discharge through the turbine used in such tests, particular attention was paid to index methods allowing to measure the relative discharge through the turbine-the index current meter method and the methods based on measuring the differential pressure between two points properly located at the turbine flow system （i.e., Winter-Kennedy method）. These methods contribute to effective reduction of the cost of optimizing the turbine that is extremely important for small hydropower plants regarding installed capacity. The paper presents selected examples of the optimization tests and experiences that arise from these tests.展开更多
This paper concerns two-person zero-sum games for a class of average-payoff continuous-time Markov processes in Polish spaces.The underlying processes are determined by transition rates that are allowed to be unbounde...This paper concerns two-person zero-sum games for a class of average-payoff continuous-time Markov processes in Polish spaces.The underlying processes are determined by transition rates that are allowed to be unbounded,and the payoff function may have neither upper nor lower bounds.We use two optimality inequalities to replace the so-called optimality equation in the previous literature.Under more general conditions,these optimality inequalities yield the existence of the value of the game and of a pair of optimal stationary strategies.Under some additional conditions we further establish the optimality equation itself.Finally,we use several examples to illustrate our results,and also to show the difference between the conditions in this paper and those in the literature.In particular,one of these examples shows that our approach is more general than all of the existing ones because it allows nonergodic Markov processes.展开更多
基金Supported by Scientific Research Fund for the Introduced Talents in Hechi University(2008QB-N001)~~
文摘[Objective] This study aimed optimize sequence-related amplified polymor- phism （SRAP）-PCR system for Paphiopedilum hirsutissimum. [Method] Using P. hir- sutissimum leaf as the material, a single-factor test was adopted to optimize the fac- tors in SRAP-PCR system, including the concentrations of dNTPs, Mg2＋, Taq poly- merase, DNA template and primers. [Result] The optimized SRAP-PCR system con- tained 2.5 μl of 10xPCR buffer, 0.15 mmol/L dNTPs, 2.0 mmol/L Mg2＋, 0.3 mmol/L primer each, 0.3 U Taq polymerase and 1 μl of DNA template. [Conclusion] This system can amplify clear and repeatable DNA profiles, which can be applied for further study about P. hirsutissimum.
基金Supported by a Program from the State Environmental Protection Administration(20061A0013)~~
文摘[Objective] By using the genomic DNA of Cymbidium faberi Rolfe as template,the factors that affect the result of ISSR-PCR reaction system were researched and the optimal system was established.[Method] The genomic DNA was extracted from C.faberi Rolfe with method of modified CTAB.Different factors which affected ISSR amplification reaction were optimized.[Result] High-quality genomic DNA was obtained from C.faberi Rolfe.And the optimal reaction system was as follows：25 μl amplification reactions system contained 2.5 μl 10 × PCR buffer,2.0 mmol/L MgCl2,60 ng template DNA,160 μmol/L dNTPs,1.25 U Taq DNA polymerase,0.4 μmol/L ISSR primer and 15.85 μl ddH2O.The optimal amplification procedures were pre-denaturing for 5 min at 94 ℃,followed by 40 cycles of denaturing for 30 s at 94 ℃,annealing for 30 s at a temperature of 2-3 ℃ lower than melting temperature of each primer pair,extension for 50 s at 72 ℃.Then extension step of 7 min at 72 ℃ was performed.[Conclusion] The optimal system could provide a favorable basis for further study on genetic diversity of C.faberi Rolfe by using ISSR molecular marker technique.
文摘[Objective] This research aimed to search a best method for extracting the genomic DNA of Cymbidium ensifolium and establish the optimized ISSR-PCR reaction system.[Method] Genomic DNA was extracted from C.ensifolium leaves by modified CTAB method.ISSR-PCR reaction system for C.ensifolium was optimized.[Result] High-quality genomic DNA was obtained from C.ensifolium.The 25 μl optimized ISSR-PCR reaction system for C.ensifolium contained 2.5 μl 10× PCR buffer,2.5 mmol/L MgCl2,240 ng template DNA,160 μmol/L dNTPs,1.25 U Taq DNA polymerase,0.4 μmol/L primer and 15.78 μl ddH2O.The optimal PCR procedures were：94 ℃ pre-denaturation for 5 min and then 40 cycles,94 ℃ denaturation for 30 s,50-60 ℃ annealing for 30 s （annealing temperature according to different primers）,72 ℃ extension for 50 s and a 72 ℃ extension for 7 min.[Conclusion] An optimized ISSR-PCR reaction system for C.ensifolium was established,which provides a basis for further study on genetic diversity of C.ensifolium by using ISSR molecular marker technique.
文摘Geographic concentration of industries is the regional- ized distribution of some industries in certain areas, which focuses on the ratio of a certain industry to the whole industries (He and Liu, 2006). In this paper we explore the improved M function of geographic concentration that adds the parameter of the number of firms according to the definition of geographic concentration of industries. The spatial distribution of the main manufacturing industries of Lanzhou urban area is evaluated based on it. The results of the evaluation imply that the spatial distribution of the main manufacturing industries is more concentrated than that of others in Lanzhou and it can absorb lots of labor forces. But the incidence, competition ability and density of the distribution of enterprises are different for each single sector, and enterprises with different production features are located closely. And three main problems are discovered. Finally, three countermeasures are put forward: locating the industrial enterprises in urban areas in a proper way through planning and policies; adjusting the industrial structure of the inner city; strengthening the local rearrangement of the existing industrial concentration areas.
基金Supported by Natural Science Foundation in Chongqing(CSTC,2006BB1336)Applied Basic Research Program of Chongqing Education Department(KJ060308)
文摘[ Objective] The paper aimed to study the optimum extraction technology of pigments from petals of Matthiola incana（ L. ） R. Br. of differ- ent colors. [Method] Petal pigments of Aida variety （white violet）, Francesco variety （ red violet） and Arabella variety （ purple violet） were ana- lyzed by colorimetric reaction method and ultraviolet-visible spectrum method, and the optimum extraction technology of flavonoid compounds from petals of them was determined by single factor experiment and orthogonal experiment. [ Result] The optimum extraction conditions of white violet were as follows： with 95% ethanol ＋ 5% HCI as solvent, solid-liquid ratio （ 1 ： 40）, extraction time （3 h）, extraction temperature （65 ℃ ）. The opti- mum extraction conditions of red violet and purple violet were as follows： with acetone as solvent, solid-liquid ratio （1：40）, extraction time （3 h）, extraction temperature （65 ℃）. [ Conclusion] The study provided theoretical foundation for further development and exploitation of pigments from M. incana（ L. ） R. Br.
文摘The paper analyzes five Finnish case companies to study the sourcing process from China. A framework of a six-stage sourcing process is developed that includes finding and choosing suppliers, making contracts and orders, performing production and quality control, planning logistics, inspecting purchases, and developing supply chain management. Each stage describes the activities from the channels of seeking suppliers to cooperative areas for further development. The case study illustrates the practices of sourcing from China are actually quite similar to the sourcing process in other countries. The study highlights that quality control is the core issue in sourcing from China. Moreover, the difference in understanding sustainable quality affects supplier screening and quality control. Close cooperation on quality control is the key to managing the supply chain to increase competitive advantage through sourcing from China.
文摘The paper presents selected experience of the authors resulting from the optimization tests of double-regulated water turbines. Among the methods for measuring the discharge through the turbine used in such tests, particular attention was paid to index methods allowing to measure the relative discharge through the turbine-the index current meter method and the methods based on measuring the differential pressure between two points properly located at the turbine flow system （i.e., Winter-Kennedy method）. These methods contribute to effective reduction of the cost of optimizing the turbine that is extremely important for small hydropower plants regarding installed capacity. The paper presents selected examples of the optimization tests and experiences that arise from these tests.
基金supported by National Natural Science Foundation and GDUPS (2010)supported by CONACyT Grant 104001
文摘This paper concerns two-person zero-sum games for a class of average-payoff continuous-time Markov processes in Polish spaces.The underlying processes are determined by transition rates that are allowed to be unbounded,and the payoff function may have neither upper nor lower bounds.We use two optimality inequalities to replace the so-called optimality equation in the previous literature.Under more general conditions,these optimality inequalities yield the existence of the value of the game and of a pair of optimal stationary strategies.Under some additional conditions we further establish the optimality equation itself.Finally,we use several examples to illustrate our results,and also to show the difference between the conditions in this paper and those in the literature.In particular,one of these examples shows that our approach is more general than all of the existing ones because it allows nonergodic Markov processes.
