苯酚废水生物毒害作用强,处理过程消耗能量大,利用微生物燃料电池技术处理苯酚废水可在水质净化的同时以电能的形式回收能量。本文以模拟苯酚废水为燃料构建并启动双室微生物燃料电池,考察电池产电特性及对苯酚废水的降解特性。结果表明...苯酚废水生物毒害作用强,处理过程消耗能量大,利用微生物燃料电池技术处理苯酚废水可在水质净化的同时以电能的形式回收能量。本文以模拟苯酚废水为燃料构建并启动双室微生物燃料电池,考察电池产电特性及对苯酚废水的降解特性。结果表明:微生物燃料电池可以利用苯酚废水为底物产电,电池稳定运行电压输出为220±10 m V,稳定输出功率密度为161.30 m W/cm~3±0.33 m W/cm~3;电池对苯酚处理效果较好,COD去除率为79.4%±2.0%,相应的苯酚污染物去除率为97.0%±2.0%;稳定运行的电池阳极微生物多样性较好。展开更多
NMR spectroscopy and X-ray crystallography are two premium methods for determining the atomic structures of macro-biomolecular complexes.Each method has unique strengths and weaknesses.While the two techniques are hig...NMR spectroscopy and X-ray crystallography are two premium methods for determining the atomic structures of macro-biomolecular complexes.Each method has unique strengths and weaknesses.While the two techniques are highly complementary,they have generally been used separately to address the structure and functions of biomolecular complexes.In this review,we emphasize that the combination of NMR spectroscopy and X-ray crystallography offers unique power for elucidating the structures of complicated protein assemblies.We demonstrate,using several recent examples from our own laboratory,that the exquisite sensitivity of NMR spectroscopy in detecting the conformational properties of individual atoms in proteins and their complexes,without any prior knowledge of conformation,is highly valuable for obtaining the high quality crystals necessary for structure determination by X-ray crystallography.Thus NMR spectroscopy,in addition to answering many unique structural biology questions that can be addressed specifically by that technique,can be exceedingly powerful in modern structural biology when combined with other techniques including X-ray crystallography and cryo-electron microscopy.展开更多
文摘苯酚废水生物毒害作用强,处理过程消耗能量大,利用微生物燃料电池技术处理苯酚废水可在水质净化的同时以电能的形式回收能量。本文以模拟苯酚废水为燃料构建并启动双室微生物燃料电池,考察电池产电特性及对苯酚废水的降解特性。结果表明:微生物燃料电池可以利用苯酚废水为底物产电,电池稳定运行电压输出为220±10 m V,稳定输出功率密度为161.30 m W/cm~3±0.33 m W/cm~3;电池对苯酚处理效果较好,COD去除率为79.4%±2.0%,相应的苯酚污染物去除率为97.0%±2.0%;稳定运行的电池阳极微生物多样性较好。
基金supported by grants from the Research Grants Council of Hong Kong to M.Z.supported by the National Major Basic Research Program of China (Grant No. 2011CB910500)+3 种基金the National Natural Science Foundation of China (Grant No. 31070657)the Knowledge Innovation Program of the Chinese Academy of Sciences (Grant No. KSCX2-YW-R-154)The NMR spectrometers used in our studies were funded by donations from the Hong Kong Jockey Club Charity Foundationthe Special Equipment Grant from RGC of Hong Kong (Grant No. SEG_HKUST06)
文摘NMR spectroscopy and X-ray crystallography are two premium methods for determining the atomic structures of macro-biomolecular complexes.Each method has unique strengths and weaknesses.While the two techniques are highly complementary,they have generally been used separately to address the structure and functions of biomolecular complexes.In this review,we emphasize that the combination of NMR spectroscopy and X-ray crystallography offers unique power for elucidating the structures of complicated protein assemblies.We demonstrate,using several recent examples from our own laboratory,that the exquisite sensitivity of NMR spectroscopy in detecting the conformational properties of individual atoms in proteins and their complexes,without any prior knowledge of conformation,is highly valuable for obtaining the high quality crystals necessary for structure determination by X-ray crystallography.Thus NMR spectroscopy,in addition to answering many unique structural biology questions that can be addressed specifically by that technique,can be exceedingly powerful in modern structural biology when combined with other techniques including X-ray crystallography and cryo-electron microscopy.
