工控图形的组态优化设计

随着工程数量的增加,系统组态成本加大显得十分突出。针对DCS图形组态的耗时费力,提出了优化解决方案。标签替换辅助工具软件综合多种技术,简化了组态过程。用图形模板简化图形操作;用每个标签代替复杂信息语句;用电子表格汇总登记指定信息。用软件对图形文本的XML语言进行编辑和信息替换,自动批量处理图形。所有替换数据信息被定义在开放的配置文本文件中,方便其修改和扩展。设计思想可作为组态优化的参考模式。

基于馈线偶的配网快速负荷均衡重构方法

为了实现配网重构的在线应用,使网络中负荷均衡,提高网络可靠性,本文提出了一种基于馈线偶的配网快速重构方法。该方法以馈线偶为单位,利用最佳转移负荷公式在馈线偶内转移负荷,以达到馈线偶内的快速负荷均衡。在此基础上,对全网进行负荷均衡化。全网均衡化按照馈线偶的负荷不均衡程度从重到轻依次进行,重构中通过对馈线偶优化标记的标定及消除,最终达到全网负荷均衡化的目的。算例分析表明本文方法快速有效,可以满足网络重构在线应用的要求。

基于馈线偶的配电网快速减小网损重构方法

作为配电网自动化高级应用模块,网络重构应满足快速性的要求而达到在线应用的目的。而许多配电网重构方法因追求全局最优或多次潮流计算而不能满足该要求。文中提出一种基于馈线偶的快速减小网损重构方法。该方法利用馈线偶内网损估算公式推导馈线偶内最佳转移负荷来寻找最优分段开关,可以避免多次潮流计算,完成馈线偶内重构。以馈线偶内重构为基础,并按照全网所有馈线偶的联络开关两侧压差的大小顺序进行全网网络重构。全网重构时对所有馈线进行"优化标定",可以起到避免重构死循环和结束全网重构的作用。最后采用13节点和185节点配电网进行了编程计算,结果表明了该方法的快速性和有效性,可以满足配电网重构在线应用的要求。

Optimization and Testing for PCR System of Rice by Orthogonal Design

[Objective] This study was to screen the economic or stable PCR system of rice and detect the generality of the selected system in different molecular markers based on PCR.[Method] With DNA extracted from rice leaves by CTAB method as the template,PCR system was optimized by L16(45)orthogonal design.[Result] Clear bands were amplified from 16 different combinations,but the amplification effects and yields had difference.The most economic and applicable system was as follows:20 ng DNA template,150 μmol/L dNT...

Optimization of SSR Reaction System for Identifying the Authenticity of Maize Hybrids

[Objective]The aim was to optimize SSR reaction system applied in the identification of authenticity of maize variety.[Method]The technical parameters of SSR including PCR reaction system,annealing temperature and electrophoresis time were optimized to identify 10 major maize varieties in Liaoning Province.[Result]The optimum PCR reaction system was：14.60 μl sterile ultrapure water,2.00 μl 10 × Buffer（Mg2＋）,1.20 μl dNTPs,0.20 μl Taq enzyme,0.50 μl each of the forward and reverse primers and1.00 μl DNA stock solution.Annealing temperature and electrophoresis time could greatly influence the results of PCR amplification.The optimal annealing temperature and electrophoresis time required for the ideal electrophoresis bands under the same conditions were different when different primers were used.[Conclusion]The system was feasible to be applied in rapid identification of authenticity of hybrid maize varieties.

Study on Establishment of cpSSR Marker Technology and Optimization of Its Reaction System in Jatropha curcas Linn

[ Objective] The aim of this study was to establish the optimum cpSSR-PCR system for Jatropha curcas Linn. [ Method] cpSSR-PCR amplification system for Jatropha curcas Linn influenced by five factors including Taq DNA polymerase, Mg^2＋ , DNA template, dNTP and primer were optimized from several levels. [ Result] The optimum concentration of 20 μl reaction system was 10 × Buffer, 2.00 mmol/L Mg^2＋ , 2 U/μl Taq DNA polymerase, 0.2 mmol/L dNTP, 0.2 μmol/L primer and 35 ng/μl DNA template. [ Conclusion] The optimum annealing temperature for cpSSR-PCR reaction system is 52 ℃, and the cpSSR reaction system is steady and reproducible.

Clustering Analysis on Large Grained Brassica napus Materials Based on the Optimized ACGM Markers

[Objective] This study aimed to develop ACGM markers for the clustering analysis of large grained Brassica napus materials. [Method] A total of 44 pairs of ACGM primers were designed according to 18 genes related to Arabidopsis grain development and their homologous rape EST sequences. After electrophoresis, 18 pairs of ACGM primers were selected for the clustering analysis of 16 larger grained samples and four fine grained samples of rapeseed. [Result] PCR result showed that 2-6 specific bands were respectively amplified by each pair of primes, and all the bands were polymorphic and repeatable, suggesting that the optimized ACGM markers were useful for clustering analysis of B. napus species. Clustering analysis revealed that the 20 rapeseed samples were divided into three clusters A, B, and C at similarity coefficient 0.6. Then, the clusters A and B were further divided into five sub clusters A1, A2, A3, B1 and B2 at similarity coefficient 0.67. [Conclusion] This study will provide theoretical and practical values for rape breeding.

一种基于色彩主题的颜色转换方法

为了实现更佳的图像转换效果,提出一种基于色彩主题的颜色转换方法。该方法能在控制色彩比率的同时维持区域的空间一致性,帮助人们实现某种意境的传达。

Establishment and Optimization of ISSR-PCR Reaction System for Cymbidium ensifolium (Linn.) Sw

[Objective] This research aimed to search a best method for extracting the genomic DNA of Cymbidium ensifolium and establish the optimized ISSR-PCR reaction system.[Method] Genomic DNA was extracted from C.ensifolium leaves by modified CTAB method.ISSR-PCR reaction system for C.ensifolium was optimized.[Result] High-quality genomic DNA was obtained from C.ensifolium.The 25 μl optimized ISSR-PCR reaction system for C.ensifolium contained 2.5 μl 10× PCR buffer,2.5 mmol/L MgCl2,240 ng template DNA,160 μmol/L dNTPs,1.25 U Taq DNA polymerase,0.4 μmol/L primer and 15.78 μl ddH2O.The optimal PCR procedures were：94 ℃ pre-denaturation for 5 min and then 40 cycles,94 ℃ denaturation for 30 s,50-60 ℃ annealing for 30 s （annealing temperature according to different primers）,72 ℃ extension for 50 s and a 72 ℃ extension for 7 min.[Conclusion] An optimized ISSR-PCR reaction system for C.ensifolium was established,which provides a basis for further study on genetic diversity of C.ensifolium by using ISSR molecular marker technique.

Fluid Flow Optimization on Semiautomatic Code Marker Base

This paper describes optimization process of nozzle cup based on a device for contactless code marking to rough and contaminated surfaces--semiautomatic code marker. During tests using the CFD （computational fluid dynamics） software different geometry of nozzle cups were compared by such parameters as pressure, velocity and direction of flow. Goal of digital experiments was to find sprayer nozzle geometry that can keep stream of paint in diameter of 10 mm on 10-20 mm distance. Main problem is to avoid low-pressure regions around the stream. The optimal geometry of nozzle cup is designed to get adjusted code dimensions on given distance