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蜂毒肽Protopolybia-MPⅢ及其优化突变体对HSV-1病毒复制周期不同阶段的抑制活性研究
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作者 孙芳 邬理莉 +3 位作者 覃陈虎 罗旭东 陈宗运 叶祥东 《湖北医药学院学报》 CAS 2024年第4期343-348,共6页
目的:研究蜂毒肽Protopolybia-MPⅢ及其优化突变体MPⅢ-3/4/7/10/14在体外对Ⅰ型单纯疱疹病毒(HSV-1)复制周期不同阶段的抑制活性。方法:通过qPCR技术和多肽分阶段孵育平台,检测蜂毒肽Protopoly⁃bia-MPⅢ及5个优化突变体在Vero细胞内对H... 目的:研究蜂毒肽Protopolybia-MPⅢ及其优化突变体MPⅢ-3/4/7/10/14在体外对Ⅰ型单纯疱疹病毒(HSV-1)复制周期不同阶段的抑制活性。方法:通过qPCR技术和多肽分阶段孵育平台,检测蜂毒肽Protopoly⁃bia-MPⅢ及5个优化突变体在Vero细胞内对HSV-1感染的预防作用,以及其对HSV-1复制周期不同阶段的影响。结果:Protopolybia-MPⅢ多肽突变体MPⅢ-4/10/14预处理Vero细胞后去除上清,能限制HSV-1感染。进一步通过多肽抗病毒作用阶段分析实验,发现野生型多肽Protopolybia-MPⅢ主要作用于HSV-1复制周期的吸附阶段,而MPⅢ-3/14主要作用于病毒复制周期的进入/融合以及进入后阶段,MPⅢ-4/7主要作用于病毒复制周期的吸附以及进入/融合阶段,MPⅢ-10主要作用于病毒复制周期的吸附以及进入后阶段。结论:本工作明确了蜂毒肽Protopolybia-MPⅢ及其突变体MPⅢ-3/4/7/10/14对HSV-1复制周期不同阶段的抑制活性,初步阐明了Protopolybia-MPⅢ多肽优化突变体抗病毒活性提高的原因,为胡蜂抗病毒多肽的分子设计和药用研究奠定基础。 展开更多
关键词 胡蜂毒液多肽 优化突变体 Ⅰ型单纯疱疹病毒 复制周期
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Flow shop rescheduling problem under rush orders 被引量:2
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作者 胡燕海 严隽琪 +1 位作者 叶飞帆 于军合 《Journal of Zhejiang University-Science A(Applied Physics & Engineering)》 SCIE EI CAS CSCD 2005年第10期1040-1046,共7页
In the environment of customization, disturbances such as rush orders and material shortages often occur in the manufacturing system, so rescheduling is necessary for the manufacturing system. The rescheduling methodo... In the environment of customization, disturbances such as rush orders and material shortages often occur in the manufacturing system, so rescheduling is necessary for the manufacturing system. The rescheduling methodology should be able to dispose of the disturbance efficiently so as to keep production going smoothly. This aims researching flow shop rescheduling problem (FSRP) necessitated by rush orders. Disjunctive graph is employed to demonstrate the FSRP. For a flow shop processing n jobs, after the original schedule has been made, and z out of n jobs have been processed in the flow shop, x rush orders come, so the original n jobs together with x rush orders should be rescheduled immediately so that the rush orders would be processed in the shortest time and the original jobs could be processed subject to some optimized criteria. The weighted mean flow time of both original jobs and rush orders is used as objective function. The weight for rush orders is much bigger than that of the original jobs, so the rush orders should be processed early in the new schedule. The ant colony optimization (ACO) algorithm used to solve the rescheduling problem has a weakness in that the search may fall into a local optimum. Mutation operation is employed to enhance the ACO performance. Numerical experiments demonstrated that the proposed algorithm has high computation repeatability and efficiency. 展开更多
关键词 Flow shop rescheduling Dynamic scheduling Rush order Ant colony optimization Mutation operation
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