在500 k V变电构架的设计中,目前国内人字形构架柱和单钢管悬臂支架柱应用较为成熟,角钢格构式变电构架应用较少。在海外工程中,角钢运输具有较大优势。结合实际工程,以埃塞俄比亚某500 k V变电构架为实例,通过根开的有效组合,对21种不...在500 k V变电构架的设计中,目前国内人字形构架柱和单钢管悬臂支架柱应用较为成熟,角钢格构式变电构架应用较少。在海外工程中,角钢运输具有较大优势。结合实际工程,以埃塞俄比亚某500 k V变电构架为实例,通过根开的有效组合,对21种不同根开的变电构架进行了全面的计算,以用钢量和混凝土用量为依据进行工程造价比较分析,得出了类似工程条件下的最优根开,以指导工程设计。当构架柱高约为30 m时,结合构架所处地理位置气候等条件,最优根开建议值为6.1 m×3.1 m,即长向根开约取为柱高的1/5,短向根开约取为柱高的1/10时,结构整体力学性能较好且最为经济。展开更多
To provide an insight into the molecular basis of heterosis, differential display of mRNA was used to analyze the difference of gene expression between wheat (Triticum aestivum L.) heterotic hybrid A, nonheterotic hyb...To provide an insight into the molecular basis of heterosis, differential display of mRNA was used to analyze the difference of gene expression between wheat (Triticum aestivum L.) heterotic hybrid A, nonheterotic hybrid B and their parental inbreds in the primary roots. By using 5′ end random primers in combination with three one-base-anchored primers, it was found that 22.5% and 22.9% of 877 total displayed cDNAs were differentially expressed between hybrid A, B and their parents, respectively. Both quantitative and qualitative differences in gene expression between hybrids and their parental inbreds were obvious, indicating that the patterns of gene expression in hybrids alter significantly as compared to their corresponding parents. On the other hand, by using MADS-box gene specific 5′ end primer for DDRT-PCR, we found that nearly all of the displayed cDNA fragments were polymorphic between hybrids and their parents, and major difference occurred in qualitative level, in which hybrid specific-expressed and silenced genes are the major two patterns, suggesting that MADS-box gene may be important for manifestation of differential gene expression and wheat heterosis. In comparison with our previous results by using seedling leaves, it is indicated that differential gene expression between hybrids and parents is dependent on the tissues tested, and more differentially expressed genes were observed in the primary roots than in the seedling leaves. Therefore, it is concluded that the expressions of both randomly displayed cDNAs and transcription factor genes, such as MADS-box, alter significantly between hybrids and their parents, which might be responsible for the observed heterosis.展开更多
[Objective] The aim was to optimized and established the regeneration system of Moringa oleifera and provided foundation for its rapid propagation and further research. [Method] Tissue culture techniques and large sca...[Objective] The aim was to optimized and established the regeneration system of Moringa oleifera and provided foundation for its rapid propagation and further research. [Method] Tissue culture techniques and large scale micropropagation of M. oleifera were studied in this paper. By means of a series of experiments, we used the leaf of aseptic seedling from M. oleifera as explants to optimize and establish the regeneration system cultured in vitro by means of direct organogenesis. [Result] It was observed that using the fresh shelled M. oleifera seeds with 0.1% mercuric chloride for 6 minutes could reach the best disinfection effect. The seed germination rate was 85%. The leaf could produce cluster buds well using the medium with MS+2.0 mg/L 6-BA+0.05 mg/L NAA, while the best proliferation condition was under MS+6-BA 1.0 mg/L+KT 0.1 rag/L+2, 4-D 2.0 mg/L+NAA 0.05 mg/L. The best rooting induction culture medium was MS+0.5 mg/L IBA, with the rooting rate as 100%. [Cenclusien] This protocol might find use in mass production of true- to-type plants and in production of transgenic plants through Agrobacterium/biolisticmediated transformation.展开更多
In ultrasonic extraction technology, optimization of technical parameters often considers extraction medium only, without including ultrasonic parameters. This paper focuses on controlling the ultrasonic extraction pr...In ultrasonic extraction technology, optimization of technical parameters often considers extraction medium only, without including ultrasonic parameters. This paper focuses on controlling the ultrasonic extraction process of puerarin, investigating the influence of ultrasonic parameters on extraction rate, and empirically analyzing the main components of Pueraria, i.e., isoflavone compounds. A method is presented combining orthogonal experi- mental design with a support vector machine and a predictive model is established for optimization of technical parameters. From the analysis with the predictive model, appropriate process parameters are achieved for higher extraction rate. With these parameters in the ultrasonic extraction of puerarin, the experimental result is satisfactory. This method is of significance to the study of extracfing root-stock plant medicines.展开更多
In this paper, we propose a parallel data assimilation module based on ensemble optimal interpolation (EnOI). We embedded the method into the full-spectral third-generation wind-wave model, WAVEWATCH III Version 3.1...In this paper, we propose a parallel data assimilation module based on ensemble optimal interpolation (EnOI). We embedded the method into the full-spectral third-generation wind-wave model, WAVEWATCH III Version 3.14, producing a wave data assimilation system. We present our preliminary experiments assimilating altimeter significant wave heights (SWH) using the EnOI-based wave assimilation system. Waters north of 15°S in the Indian Ocean and South China Sea were chosen as the target computational domain, which was two-way nested into the global implementation of the WAVEWATCH III. The wave model was forced by six-hourly ocean surface wind velocities from the cross-calibrated multi-platform wind vector dataset. The assimilation used along-track SWH data from the Jason-2 altimeter. We evaluated the effect of the assimilation on the analyses and hindcasts, and found that our technique was effective. Although there was a considerable mean bias in the control SWHs, a month-long consecutive assimilation reduced the bias by approximately 84% and the root mean-square error (RMSE) by approximately 65%. Improvements in the SWH RMSE for both the analysis and hindcast periods were more significant in July than January, because of the monsoon climate. The improvement in model skill persisted for up to 48 h in July. Furthermore, the SWH data assimilation had the greatest impact in areas and seasons where and when the sea-states were dominated by swells.展开更多
Arbuscular mycorrhizal fungi(AMF)can increase host plant nutrient uptake via their mycelium,thus promoting plant growth.AMF have always been associated with successful invasion of most exotic plant species.However,kno...Arbuscular mycorrhizal fungi(AMF)can increase host plant nutrient uptake via their mycelium,thus promoting plant growth.AMF have always been associated with successful invasion of most exotic plant species.However,knowledge regarding how AMF affect the success of plant invasion remains limited.Exotic Ambrosia artemisiifolia is an invasive and mycorrhizal plant species.A long-term field experiment was conducted to examine the differences in AMF diversity and composition in the roots of A.artemisiifolia and Setaria viridis subjected to interspecific competition during growth.A greenhouse experiment was also performed to test the effect of Funneliformis mosseae on the growth of these two species.Ambrosia artemisiifolia invasion caused AMF diversity to change in native S.viridis roots.Meanwhile,the relative abundance of F.mosseae was significantly higher in the roots of A.artemisiifolia than in those of S.viridis.The higher AMF colonization rate in the exotic species(A.artemisiifolia)than in the native species(S.viridis)was found in both the field and greenhouse experiments.The greenhouse experiment possibly provided that AMF advantaged to the growth of A.artemisiifolia,by influencing its photosynthetic capacity as well as its phosphorus and potassium absorption.These observations highlight the important relationship of AMF with the successful invasion of A.artemisiifolia.展开更多
文摘在500 k V变电构架的设计中,目前国内人字形构架柱和单钢管悬臂支架柱应用较为成熟,角钢格构式变电构架应用较少。在海外工程中,角钢运输具有较大优势。结合实际工程,以埃塞俄比亚某500 k V变电构架为实例,通过根开的有效组合,对21种不同根开的变电构架进行了全面的计算,以用钢量和混凝土用量为依据进行工程造价比较分析,得出了类似工程条件下的最优根开,以指导工程设计。当构架柱高约为30 m时,结合构架所处地理位置气候等条件,最优根开建议值为6.1 m×3.1 m,即长向根开约取为柱高的1/5,短向根开约取为柱高的1/10时,结构整体力学性能较好且最为经济。
文摘To provide an insight into the molecular basis of heterosis, differential display of mRNA was used to analyze the difference of gene expression between wheat (Triticum aestivum L.) heterotic hybrid A, nonheterotic hybrid B and their parental inbreds in the primary roots. By using 5′ end random primers in combination with three one-base-anchored primers, it was found that 22.5% and 22.9% of 877 total displayed cDNAs were differentially expressed between hybrid A, B and their parents, respectively. Both quantitative and qualitative differences in gene expression between hybrids and their parental inbreds were obvious, indicating that the patterns of gene expression in hybrids alter significantly as compared to their corresponding parents. On the other hand, by using MADS-box gene specific 5′ end primer for DDRT-PCR, we found that nearly all of the displayed cDNA fragments were polymorphic between hybrids and their parents, and major difference occurred in qualitative level, in which hybrid specific-expressed and silenced genes are the major two patterns, suggesting that MADS-box gene may be important for manifestation of differential gene expression and wheat heterosis. In comparison with our previous results by using seedling leaves, it is indicated that differential gene expression between hybrids and parents is dependent on the tissues tested, and more differentially expressed genes were observed in the primary roots than in the seedling leaves. Therefore, it is concluded that the expressions of both randomly displayed cDNAs and transcription factor genes, such as MADS-box, alter significantly between hybrids and their parents, which might be responsible for the observed heterosis.
基金National Natural Science Foundation of China(21165008)~~
文摘[Objective] The aim was to optimized and established the regeneration system of Moringa oleifera and provided foundation for its rapid propagation and further research. [Method] Tissue culture techniques and large scale micropropagation of M. oleifera were studied in this paper. By means of a series of experiments, we used the leaf of aseptic seedling from M. oleifera as explants to optimize and establish the regeneration system cultured in vitro by means of direct organogenesis. [Result] It was observed that using the fresh shelled M. oleifera seeds with 0.1% mercuric chloride for 6 minutes could reach the best disinfection effect. The seed germination rate was 85%. The leaf could produce cluster buds well using the medium with MS+2.0 mg/L 6-BA+0.05 mg/L NAA, while the best proliferation condition was under MS+6-BA 1.0 mg/L+KT 0.1 rag/L+2, 4-D 2.0 mg/L+NAA 0.05 mg/L. The best rooting induction culture medium was MS+0.5 mg/L IBA, with the rooting rate as 100%. [Cenclusien] This protocol might find use in mass production of true- to-type plants and in production of transgenic plants through Agrobacterium/biolisticmediated transformation.
基金Supported by the National Natural Science Foundation of China(21146009,21376014)
文摘In ultrasonic extraction technology, optimization of technical parameters often considers extraction medium only, without including ultrasonic parameters. This paper focuses on controlling the ultrasonic extraction process of puerarin, investigating the influence of ultrasonic parameters on extraction rate, and empirically analyzing the main components of Pueraria, i.e., isoflavone compounds. A method is presented combining orthogonal experi- mental design with a support vector machine and a predictive model is established for optimization of technical parameters. From the analysis with the predictive model, appropriate process parameters are achieved for higher extraction rate. With these parameters in the ultrasonic extraction of puerarin, the experimental result is satisfactory. This method is of significance to the study of extracfing root-stock plant medicines.
基金Supported by the National Special Research Fund for Non-Profit Marine Sector(Nos.201005033,201105002)the National High Technology Research and Development Program of China(863 Program)(No.2012AA091801)+1 种基金the National Natural Science Foundation of China(No.U1133001)the NSFC-Shandong Joint Fund for Marine Science Research Centers(No.U1406401)
文摘In this paper, we propose a parallel data assimilation module based on ensemble optimal interpolation (EnOI). We embedded the method into the full-spectral third-generation wind-wave model, WAVEWATCH III Version 3.14, producing a wave data assimilation system. We present our preliminary experiments assimilating altimeter significant wave heights (SWH) using the EnOI-based wave assimilation system. Waters north of 15°S in the Indian Ocean and South China Sea were chosen as the target computational domain, which was two-way nested into the global implementation of the WAVEWATCH III. The wave model was forced by six-hourly ocean surface wind velocities from the cross-calibrated multi-platform wind vector dataset. The assimilation used along-track SWH data from the Jason-2 altimeter. We evaluated the effect of the assimilation on the analyses and hindcasts, and found that our technique was effective. Although there was a considerable mean bias in the control SWHs, a month-long consecutive assimilation reduced the bias by approximately 84% and the root mean-square error (RMSE) by approximately 65%. Improvements in the SWH RMSE for both the analysis and hindcast periods were more significant in July than January, because of the monsoon climate. The improvement in model skill persisted for up to 48 h in July. Furthermore, the SWH data assimilation had the greatest impact in areas and seasons where and when the sea-states were dominated by swells.
基金funded by the National Natural Science Foundation of China(grant no.31972343 and 31372000)Hebei National Natural Science Foundation(C2019201059)College of Life Science,Institute of Life Science and Green Development,Hebei University.
文摘Arbuscular mycorrhizal fungi(AMF)can increase host plant nutrient uptake via their mycelium,thus promoting plant growth.AMF have always been associated with successful invasion of most exotic plant species.However,knowledge regarding how AMF affect the success of plant invasion remains limited.Exotic Ambrosia artemisiifolia is an invasive and mycorrhizal plant species.A long-term field experiment was conducted to examine the differences in AMF diversity and composition in the roots of A.artemisiifolia and Setaria viridis subjected to interspecific competition during growth.A greenhouse experiment was also performed to test the effect of Funneliformis mosseae on the growth of these two species.Ambrosia artemisiifolia invasion caused AMF diversity to change in native S.viridis roots.Meanwhile,the relative abundance of F.mosseae was significantly higher in the roots of A.artemisiifolia than in those of S.viridis.The higher AMF colonization rate in the exotic species(A.artemisiifolia)than in the native species(S.viridis)was found in both the field and greenhouse experiments.The greenhouse experiment possibly provided that AMF advantaged to the growth of A.artemisiifolia,by influencing its photosynthetic capacity as well as its phosphorus and potassium absorption.These observations highlight the important relationship of AMF with the successful invasion of A.artemisiifolia.