To provide an insight into the molecular basis of heterosis, differential display of mRNA was used to analyze the difference of gene expression between wheat (Triticum aestivum L.) heterotic hybrid A, nonheterotic hyb...To provide an insight into the molecular basis of heterosis, differential display of mRNA was used to analyze the difference of gene expression between wheat (Triticum aestivum L.) heterotic hybrid A, nonheterotic hybrid B and their parental inbreds in the primary roots. By using 5′ end random primers in combination with three one-base-anchored primers, it was found that 22.5% and 22.9% of 877 total displayed cDNAs were differentially expressed between hybrid A, B and their parents, respectively. Both quantitative and qualitative differences in gene expression between hybrids and their parental inbreds were obvious, indicating that the patterns of gene expression in hybrids alter significantly as compared to their corresponding parents. On the other hand, by using MADS-box gene specific 5′ end primer for DDRT-PCR, we found that nearly all of the displayed cDNA fragments were polymorphic between hybrids and their parents, and major difference occurred in qualitative level, in which hybrid specific-expressed and silenced genes are the major two patterns, suggesting that MADS-box gene may be important for manifestation of differential gene expression and wheat heterosis. In comparison with our previous results by using seedling leaves, it is indicated that differential gene expression between hybrids and parents is dependent on the tissues tested, and more differentially expressed genes were observed in the primary roots than in the seedling leaves. Therefore, it is concluded that the expressions of both randomly displayed cDNAs and transcription factor genes, such as MADS-box, alter significantly between hybrids and their parents, which might be responsible for the observed heterosis.展开更多
Hybrid rice Xinhunyou No. 6 could be cultivated by mixing male and fe- male parents and performing seedling raising followed by transplanting, mechanical planting or mechanical direct seeding according to the producti...Hybrid rice Xinhunyou No. 6 could be cultivated by mixing male and fe- male parents and performing seedling raising followed by transplanting, mechanical planting or mechanical direct seeding according to the production methods of com- mercial rice. During flowering stage, leaf cutting, application of ＂920＂ and pollination were conducted; after pollination, bentazon with a certain concentration was sprayed to kill the male parent; and the hybrid rice was harvested mechanically. Before storage, color separation was performed to remove little remaining male parent, thereby achieving whole-process mechanization of hybrid rice seed production. This study introduced mechanized seed production of Xinhunyou No. 6 through mixed- seeding from the aspects including mechanical direct seeding, seeding raising fol- lowed by transplanting and mechanical planting.展开更多
Unlike the existing resonance region radar systems （RRRS ） that transmit the orthogonal frequency division multiplexing （OFDM）multi-carrier waveform,the dense multi-carrier （DMC）radar waveform which has a narrow...Unlike the existing resonance region radar systems （RRRS ） that transmit the orthogonal frequency division multiplexing （OFDM）multi-carrier waveform,the dense multi-carrier （DMC）radar waveform which has a narrower frequency interval than the traditional OFDM waveform is proposed.Therefore,in the same frequency bandwidth,the DMC waveform contains more sub-carriers and provides more frequency diversity.Additionally,to further improve detection performance,a novel optimal weight accumulation target detection （OWATD）method is proposed,where the echo electromagnetic waves at different frequencies are accumulated with the optimal weight coefficients.Then,with the signal-to-noise ratio （SNR）of echo waveform approaching infinity,the asymptotic detection performance is analyzed, and the condition that the OWATD method with the DMC outperforms the matched filter with the OFDM is presented.Simulation results show that the DMC outperforms the OFDM in the target detection performance,and the OWATD method can further improve the detection performance of the traditional methods with both the OFDM and DMC radar waveform.展开更多
Maize （Zea raays L.） is one of the most important crops because of the remarkable properties of its hybrid, which is responsible for the high commercial value of hybrid maize. The genetic basis of heterosis （hybrid...Maize （Zea raays L.） is one of the most important crops because of the remarkable properties of its hybrid, which is responsible for the high commercial value of hybrid maize. The genetic basis of heterosis （hybrid vigor） is not well understood. A differential display technique was performed to identify genes with differential expression across twelve maize inbred lines and thirty-three hybrids during ear development. An incomplete diallel design was used to investigate the relationship between the global framework of differential gene expression and heterosis. It was found that the genes belonging to MONO pattern （i.e., genes expressed in both parental lines and in hybrid） was the highest in percentage among the total five patterns and illustrated that the properties of differentially expressed genes are not entirely responsible for heterosis. Furthermore,a larger number of differentially expressed genes in hybrid, which serves as a major reservoir for generating novel phenotypes that exhibit heterosis of certain agronomic traits during early development and differentiation of maize ear. Moreover, there were some silent genesin hybrids that are responsible for the arrest or abortion of spikelets and for the increase in kernels weight.展开更多
Optimization of antenna array pattern used in a spaceborne Synthetic Aperture Radar (SAR) system is considered in this study. A robust evolutionary algorithm, Non-dominated Sorting Genetic Algorithms (the improved NS...Optimization of antenna array pattern used in a spaceborne Synthetic Aperture Radar (SAR) system is considered in this study. A robust evolutionary algorithm, Non-dominated Sorting Genetic Algorithms (the improved NSGA-Ⅱ), is applied on a spaceborne SAR antenna pattern design. The system consists of two objective functions with two constraints. Pareto fronts are generated as a result of multi-objective optimization. After being validated by a test problem ZDT4, the algorithms are used to synthesize spaceborne SAR antenna radiation pattern. The good results with low Ambi- guity-to-Signal Ratio (ASR) and high directivity are obtained in the paper.展开更多
In order to improve the extracellular endo-1,4-β-mannosidase(MAN) activity of recombinant Pichia pastoris, optimization of signal peptides was investigated. At first, five potential signal peptides(W1, MF4 I, INU1 A,...In order to improve the extracellular endo-1,4-β-mannosidase(MAN) activity of recombinant Pichia pastoris, optimization of signal peptides was investigated. At first, five potential signal peptides(W1, MF4 I, INU1 A, αpre, HFBI) were chosen to be analyzed by Signal P 4.0, among which W1 was designed. Then, the widely used signal peptide α-factor in expression vector p GAPZαA was replaced by those five signal peptides to reconstruct five new expression vectors. MAN activity was assayed after expression vectors were transformed into Pichia pastoris. The data show that the relative efficiencies of W1, MF4 I, INU1 A, αpre, and HFBI signal peptides are 23.5%, 203.5%, 0, 79.7%, and 120.3% compared with α-factor, respectively. The further gene copy number determination by the quantitative real-time PCR reveals that the MAN activities mediated by α-factor from 1 to 6 gene copy number levels are 12.95, 43.33, 126.63, 173.53, 103.23 and 88.63 U/m L, while those mediated by MF4 I are 79.22, 133.89, 260.14, 347.5, 206.15 and 181.89 U/m L, respectively. The maximum MAN activity reached 347.5 U/m L with 4 gene copies mediated by MF4 I. These results indicate that replacing the signal peptide α-factor with MF4 I and increasing MAN gene copies to a proper number can greatly improve the secretory expression of MAN.展开更多
This paper presents a method to reconstruct 3-D models of trees from terrestrial laser scan(TLS)point clouds.This method uses the weighted locally optimal projection(WLOP)and the AdTree method to reconstruct detailed ...This paper presents a method to reconstruct 3-D models of trees from terrestrial laser scan(TLS)point clouds.This method uses the weighted locally optimal projection(WLOP)and the AdTree method to reconstruct detailed 3-D tree models.To improve its representation accuracy,the WLOP algorithm is introduced to consolidate the point cloud.Its reconstruction accuracy is tested using a dataset of ten trees,and the one-sided Hausdorff distances between the input point clouds and the resulting 3-D models are measured.The experimental results show that the optimal projection modeling method has an average one-sided Hausdorff distance(mean)lower by 30.74%and 6.43%compared with AdTree and AdQSM methods,respectively.Furthermore,it has an average one-sided Hausdorff distance(RMS)lower by 29.95%and 12.28%compared with AdTree and AdQSM methods.Results show that the 3-D model generated fits closely to the input point cloud data and ensures a high geometrical accuracy.展开更多
In developing countries, land productivity involves little market, where the agricultural land use is mainly determined by the food demands as well as the land suitability. The land use pattern will not ensure everywh...In developing countries, land productivity involves little market, where the agricultural land use is mainly determined by the food demands as well as the land suitability. The land use pattern will not ensure everywhere enough land for certain cropping if spatial allocation just according to land use suitability. To solve this problem, a subzone and a pre-allocation for each land use are added in spatial allocation module, and land use suitability and area optimi- zation module are incorporated to constitute a whole agricultural land use optimal allocation （ALUOA） system. The system is developed on the platform .Net 2005 using ArcGIS Engine （version 9.2） and C# language, and is tested and validated in Yili watershed of Xinjiang Region on the newly reclaimed area. In the case study, with the help of soil data obtained from 69 points sampled in the fieldwork in 2008, main river data supplied by the Department of Water Resources of Xinjiang Uygur Autonomous Region in China, and temperature data provided by Data Center for Resources and Environmental Sciences, Chinese Academy of Sciences, land use suitability on eight common crops are evaluated one by one using linear weighted summation method in the land use suitability model. The linear pro- gramming （LP） model in area optimization model succeeds to give out land area target of each crop under three scenarios. At last, the land use targets are allotted in space both with a six subzone file and without a subzone file. The resuits show that the land use maps with a subzone not only ensure every part has enough land for every crop, but also gives a more fragmental land use pattern, with about 87.99% and 135.92% more patches than the one without, while at the expense of loss between 15.30% and 19.53% in the overall suitability at the same time.展开更多
文摘To provide an insight into the molecular basis of heterosis, differential display of mRNA was used to analyze the difference of gene expression between wheat (Triticum aestivum L.) heterotic hybrid A, nonheterotic hybrid B and their parental inbreds in the primary roots. By using 5′ end random primers in combination with three one-base-anchored primers, it was found that 22.5% and 22.9% of 877 total displayed cDNAs were differentially expressed between hybrid A, B and their parents, respectively. Both quantitative and qualitative differences in gene expression between hybrids and their parental inbreds were obvious, indicating that the patterns of gene expression in hybrids alter significantly as compared to their corresponding parents. On the other hand, by using MADS-box gene specific 5′ end primer for DDRT-PCR, we found that nearly all of the displayed cDNA fragments were polymorphic between hybrids and their parents, and major difference occurred in qualitative level, in which hybrid specific-expressed and silenced genes are the major two patterns, suggesting that MADS-box gene may be important for manifestation of differential gene expression and wheat heterosis. In comparison with our previous results by using seedling leaves, it is indicated that differential gene expression between hybrids and parents is dependent on the tissues tested, and more differentially expressed genes were observed in the primary roots than in the seedling leaves. Therefore, it is concluded that the expressions of both randomly displayed cDNAs and transcription factor genes, such as MADS-box, alter significantly between hybrids and their parents, which might be responsible for the observed heterosis.
基金Supported by Science and Technology Innovation Team of Anhui Academy of Agricultural Sciences(15C0108)Subject Construction Project of Anhui Academy of Agricultural Sciences(14A0102)Science and Technology Support Program of Ministry of Science and Technology(2012BAD07B01-3)~~
文摘Hybrid rice Xinhunyou No. 6 could be cultivated by mixing male and fe- male parents and performing seedling raising followed by transplanting, mechanical planting or mechanical direct seeding according to the production methods of com- mercial rice. During flowering stage, leaf cutting, application of ＂920＂ and pollination were conducted; after pollination, bentazon with a certain concentration was sprayed to kill the male parent; and the hybrid rice was harvested mechanically. Before storage, color separation was performed to remove little remaining male parent, thereby achieving whole-process mechanization of hybrid rice seed production. This study introduced mechanized seed production of Xinhunyou No. 6 through mixed- seeding from the aspects including mechanical direct seeding, seeding raising fol- lowed by transplanting and mechanical planting.
基金The National Natural Science Foundation of China(No.61271204)the National Key Technology R&D Program during the 12th Five-Year Plan Period(No.2012BAH15B00)
文摘Unlike the existing resonance region radar systems （RRRS ） that transmit the orthogonal frequency division multiplexing （OFDM）multi-carrier waveform,the dense multi-carrier （DMC）radar waveform which has a narrower frequency interval than the traditional OFDM waveform is proposed.Therefore,in the same frequency bandwidth,the DMC waveform contains more sub-carriers and provides more frequency diversity.Additionally,to further improve detection performance,a novel optimal weight accumulation target detection （OWATD）method is proposed,where the echo electromagnetic waves at different frequencies are accumulated with the optimal weight coefficients.Then,with the signal-to-noise ratio （SNR）of echo waveform approaching infinity,the asymptotic detection performance is analyzed, and the condition that the OWATD method with the DMC outperforms the matched filter with the OFDM is presented.Simulation results show that the DMC outperforms the OFDM in the target detection performance,and the OWATD method can further improve the detection performance of the traditional methods with both the OFDM and DMC radar waveform.
文摘Maize （Zea raays L.） is one of the most important crops because of the remarkable properties of its hybrid, which is responsible for the high commercial value of hybrid maize. The genetic basis of heterosis （hybrid vigor） is not well understood. A differential display technique was performed to identify genes with differential expression across twelve maize inbred lines and thirty-three hybrids during ear development. An incomplete diallel design was used to investigate the relationship between the global framework of differential gene expression and heterosis. It was found that the genes belonging to MONO pattern （i.e., genes expressed in both parental lines and in hybrid） was the highest in percentage among the total five patterns and illustrated that the properties of differentially expressed genes are not entirely responsible for heterosis. Furthermore,a larger number of differentially expressed genes in hybrid, which serves as a major reservoir for generating novel phenotypes that exhibit heterosis of certain agronomic traits during early development and differentiation of maize ear. Moreover, there were some silent genesin hybrids that are responsible for the arrest or abortion of spikelets and for the increase in kernels weight.
文摘Optimization of antenna array pattern used in a spaceborne Synthetic Aperture Radar (SAR) system is considered in this study. A robust evolutionary algorithm, Non-dominated Sorting Genetic Algorithms (the improved NSGA-Ⅱ), is applied on a spaceborne SAR antenna pattern design. The system consists of two objective functions with two constraints. Pareto fronts are generated as a result of multi-objective optimization. After being validated by a test problem ZDT4, the algorithms are used to synthesize spaceborne SAR antenna radiation pattern. The good results with low Ambi- guity-to-Signal Ratio (ASR) and high directivity are obtained in the paper.
基金Project(13JJ9002)supported by Hunan Provincial Natural Science Foundation of ChinaProject(2012XK4081)supported by the Key Science Technology Plan Project of Hunan Provincial Science&Technology Department,ChinaProject(CX2012B124)supported by the Graduate Degree Thesis Innovation Program of Hunan Province,China
文摘In order to improve the extracellular endo-1,4-β-mannosidase(MAN) activity of recombinant Pichia pastoris, optimization of signal peptides was investigated. At first, five potential signal peptides(W1, MF4 I, INU1 A, αpre, HFBI) were chosen to be analyzed by Signal P 4.0, among which W1 was designed. Then, the widely used signal peptide α-factor in expression vector p GAPZαA was replaced by those five signal peptides to reconstruct five new expression vectors. MAN activity was assayed after expression vectors were transformed into Pichia pastoris. The data show that the relative efficiencies of W1, MF4 I, INU1 A, αpre, and HFBI signal peptides are 23.5%, 203.5%, 0, 79.7%, and 120.3% compared with α-factor, respectively. The further gene copy number determination by the quantitative real-time PCR reveals that the MAN activities mediated by α-factor from 1 to 6 gene copy number levels are 12.95, 43.33, 126.63, 173.53, 103.23 and 88.63 U/m L, while those mediated by MF4 I are 79.22, 133.89, 260.14, 347.5, 206.15 and 181.89 U/m L, respectively. The maximum MAN activity reached 347.5 U/m L with 4 gene copies mediated by MF4 I. These results indicate that replacing the signal peptide α-factor with MF4 I and increasing MAN gene copies to a proper number can greatly improve the secretory expression of MAN.
基金supported in part by the National Natural Science Foundation of China(Nos.42271343,42177387)the Fund of State Key Laboratory of Remote Sensing Information and Image Analysis Technology of Beijing Research Institute of Uranium Geology under(No.6142A010403)
文摘This paper presents a method to reconstruct 3-D models of trees from terrestrial laser scan(TLS)point clouds.This method uses the weighted locally optimal projection(WLOP)and the AdTree method to reconstruct detailed 3-D tree models.To improve its representation accuracy,the WLOP algorithm is introduced to consolidate the point cloud.Its reconstruction accuracy is tested using a dataset of ten trees,and the one-sided Hausdorff distances between the input point clouds and the resulting 3-D models are measured.The experimental results show that the optimal projection modeling method has an average one-sided Hausdorff distance(mean)lower by 30.74%and 6.43%compared with AdTree and AdQSM methods,respectively.Furthermore,it has an average one-sided Hausdorff distance(RMS)lower by 29.95%and 12.28%compared with AdTree and AdQSM methods.Results show that the 3-D model generated fits closely to the input point cloud data and ensures a high geometrical accuracy.
基金Under the auspices of National Natural Science Foundation of China (No. 41001108, 41071065)Beijing Municipal Natural Science Foundation (No. 9113029)
文摘In developing countries, land productivity involves little market, where the agricultural land use is mainly determined by the food demands as well as the land suitability. The land use pattern will not ensure everywhere enough land for certain cropping if spatial allocation just according to land use suitability. To solve this problem, a subzone and a pre-allocation for each land use are added in spatial allocation module, and land use suitability and area optimi- zation module are incorporated to constitute a whole agricultural land use optimal allocation （ALUOA） system. The system is developed on the platform .Net 2005 using ArcGIS Engine （version 9.2） and C# language, and is tested and validated in Yili watershed of Xinjiang Region on the newly reclaimed area. In the case study, with the help of soil data obtained from 69 points sampled in the fieldwork in 2008, main river data supplied by the Department of Water Resources of Xinjiang Uygur Autonomous Region in China, and temperature data provided by Data Center for Resources and Environmental Sciences, Chinese Academy of Sciences, land use suitability on eight common crops are evaluated one by one using linear weighted summation method in the land use suitability model. The linear pro- gramming （LP） model in area optimization model succeeds to give out land area target of each crop under three scenarios. At last, the land use targets are allotted in space both with a six subzone file and without a subzone file. The resuits show that the land use maps with a subzone not only ensure every part has enough land for every crop, but also gives a more fragmental land use pattern, with about 87.99% and 135.92% more patches than the one without, while at the expense of loss between 15.30% and 19.53% in the overall suitability at the same time.
