The high-molecular-weight (HMW) glutenin subunits and their coding genes from Aegilops umbellulata Zhuk. (UU, 2n = 2x = 14) were characterized using SDS-PAGE analysis and molecular approaches. SDS-PAGE analysis showed...The high-molecular-weight (HMW) glutenin subunits and their coding genes from Aegilops umbellulata Zhuk. (UU, 2n = 2x = 14) were characterized using SDS-PAGE analysis and molecular approaches. SDS-PAGE analysis showed that the 1Ux subunits from four different accessions possessed electrophoretic mobilities close to, or slower than, that displayed by the 1Dx2.2 subunit of common wheat. The electrophoretic mobilities of the 1Uy subunits were generally similar to those shown by the 1Dy subunits of common wheat. The complete open reading frames of the 1Ux and 1Uy genes were amplified by PCR and subsequently cloned and sequenced. Amino acid sequence comparisons suggested that the primary structure of the 1Ux and 1Uy subunits were identical to that of published HMW glutenin subunits from related species, Phylogenetic analysis indicated that the HMW glutenin subunits of Ae. umbellulata were most closely related to those encoded by the D genome of Triticeae.展开更多
This paper mainly summarizes recent advances in the classification of Bursaphelenchus species, introduces several common methods of molecular biologi- cal detection and identification methods, and analyzes the advanta...This paper mainly summarizes recent advances in the classification of Bursaphelenchus species, introduces several common methods of molecular biologi- cal detection and identification methods, and analyzes the advantages and disad- vantages of these methods.展开更多
A new 2-D variational method is proposed to calculate the vibrational energy levels of the symmetric P-H stretching vibration （vl） and the symmetric umbrella vibration （inversion vibration） （v2） of PH3^＋（^～X...A new 2-D variational method is proposed to calculate the vibrational energy levels of the symmetric P-H stretching vibration （vl） and the symmetric umbrella vibration （inversion vibration） （v2） of PH3^＋（^～X^2A″2） that has the tunneling effect. Because the symmetric internal Cartesian coordinates were employed in the calculations, the kinetic energy operator is very simple and the inversion vibrational mode is well characterized. In comparison with the often used I-D model to calculate the inversion vibrational energy levels, this 2-D method does not require an assmnption of reduced mass, and the interactions between the vl and v2 vibrational modes are taken into consideration. The calculated vibrational energy levels of PH3^＋ are the first reported 2-D calculation, and the average deviation to the experimental data is less than 3 cm^-1 for the first seven inversion vibrational energy levels. This method has also been applied to calculate the vibrational energy levels of NH3. The application to NH3 is less successful, which shows some limitations of the method compared with a full dimension computation.展开更多
In order to realize safe and accurate homing of parafoil system,a multiphase homing trajectory planning scheme is proposed according to the maneuverability and basic flight characteristics of the vehicle.In this scena...In order to realize safe and accurate homing of parafoil system,a multiphase homing trajectory planning scheme is proposed according to the maneuverability and basic flight characteristics of the vehicle.In this scenario,on the basis of geometric relationship of each phase trajectory,the problem of trajectory planning is transformed to parameter optimizing,and then auxiliary population-based quantum differential evolution algorithm(AP-QDEA)is applied as a tool to optimize the objective function,and the design parameters of the whole homing trajectory are obtained.The proposed AP-QDEA combines the strengths of differential evolution algorithm(DEA)and quantum evolution algorithm(QEA),and the notion of auxiliary population is introduced into the proposed algorithm to improve the searching precision and speed.The simulation results show that the proposed AP-QDEA is proven its superior in both effectiveness and efficiency by solving a set of benchmark problems,and the multiphase homing scheme can fulfill the requirement of fixed-points and upwind landing in the process of homing which is simple in control and facile in practice as well.展开更多
In this study, to explore the radiation protection effects of Lyophyllum Decastes Sing （LDS）, a hot distilled-water extract of LDS was orally administered at a dosage of 250mg/kg every other day for a period of 2 we...In this study, to explore the radiation protection effects of Lyophyllum Decastes Sing （LDS）, a hot distilled-water extract of LDS was orally administered at a dosage of 250mg/kg every other day for a period of 2 weeks in irradiated mice. An automatic blood cell counter was used to measure white blood cells （lymphocytes, monocyte, and granulocytes） one day before X-ray irradiation, and 3 hours, 12 hours, 24 hours, 3 days, 7 days, 15 days and 30 days after irradiation. The Dunnett test was used to examine statistical significance of differences. The peripheral blood cell counts in the Lyophyllum-administered non-irradiation group revealed an increase in the numbers of ieukocytes, lymphocytes and monocytes. For 2 Gy whole body radiation, a significant statistical difference was found between the X-ray group and the Lyophyllum plus X-ray group in the numbers of leukocytes, lymphocytes and monocytes. The results suggest that Lyophyllum restrains blood cell-count falling after irradiation, which is probably mediated at least in part by hemopoietic function, and NK and LAK activities seems to play a role in preventing secondary irffections associated with irradiation.展开更多
Septoria carvi belong to the fungi recently detected on caraway plants in the Polish climatic conditions. In many European countries, this fungus causes significant losses in caraway cultivation and decreases the qual...Septoria carvi belong to the fungi recently detected on caraway plants in the Polish climatic conditions. In many European countries, this fungus causes significant losses in caraway cultivation and decreases the quality and quantity of the herb material. The aim of the present work was to study the fungus harmfulness towards the sowing materials, stems, leaves and umbels of caraway. The pathogenicity tests according to Koch＇s postulates were carried out for isolates ofS. carvi using post-culture liquids and a water suspension of conidia to inoculate the plants＇ parts. The studies were conducted in the laboratory, the climatic chamber and in field conditions. S. carvi caused disease symptoms in the form of spots and necrosis on all tested plants organs. The infection was followed by natural openings what was shown by scanning electron microscope （SEM） microscopic observations. The efficiency of inoculation increased injuries and anatomical dipping of plant epidermal tissue. The study showed the ability for asymptomatic S. carvi colonization of inoculated plants＇ parts. The performed pathogenicity tests and microscopic observations SEM showed that the tested isolates of S. carvi were occasional pathogens of caraway.展开更多
文摘The high-molecular-weight (HMW) glutenin subunits and their coding genes from Aegilops umbellulata Zhuk. (UU, 2n = 2x = 14) were characterized using SDS-PAGE analysis and molecular approaches. SDS-PAGE analysis showed that the 1Ux subunits from four different accessions possessed electrophoretic mobilities close to, or slower than, that displayed by the 1Dx2.2 subunit of common wheat. The electrophoretic mobilities of the 1Uy subunits were generally similar to those shown by the 1Dy subunits of common wheat. The complete open reading frames of the 1Ux and 1Uy genes were amplified by PCR and subsequently cloned and sequenced. Amino acid sequence comparisons suggested that the primary structure of the 1Ux and 1Uy subunits were identical to that of published HMW glutenin subunits from related species, Phylogenetic analysis indicated that the HMW glutenin subunits of Ae. umbellulata were most closely related to those encoded by the D genome of Triticeae.
基金Supported by Science Research Project of Ningbo Entry-Exit Inspection and Quarantine Bureau(YK12-2013)~~
文摘This paper mainly summarizes recent advances in the classification of Bursaphelenchus species, introduces several common methods of molecular biologi- cal detection and identification methods, and analyzes the advantages and disad- vantages of these methods.
基金This work is supported by the National Key Basic Program of China （No.2010CB922900） and the National Natural Science Foundation of China （No.91021008, No.i1274196, and No.iiiii120061）.
文摘A new 2-D variational method is proposed to calculate the vibrational energy levels of the symmetric P-H stretching vibration （vl） and the symmetric umbrella vibration （inversion vibration） （v2） of PH3^＋（^～X^2A″2） that has the tunneling effect. Because the symmetric internal Cartesian coordinates were employed in the calculations, the kinetic energy operator is very simple and the inversion vibrational mode is well characterized. In comparison with the often used I-D model to calculate the inversion vibrational energy levels, this 2-D method does not require an assmnption of reduced mass, and the interactions between the vl and v2 vibrational modes are taken into consideration. The calculated vibrational energy levels of PH3^＋ are the first reported 2-D calculation, and the average deviation to the experimental data is less than 3 cm^-1 for the first seven inversion vibrational energy levels. This method has also been applied to calculate the vibrational energy levels of NH3. The application to NH3 is less successful, which shows some limitations of the method compared with a full dimension computation.
基金Project(61273138) supported by the National Natural Science Foundation of ChinaProjects(KJ2016A169,KJ2015A242) supported by the University Natural Science Research Key Project of Anhui Province,ChinaProject(ZRC2014444) supported by the Talents Program of Anhui Science and Technology University,China
文摘In order to realize safe and accurate homing of parafoil system,a multiphase homing trajectory planning scheme is proposed according to the maneuverability and basic flight characteristics of the vehicle.In this scenario,on the basis of geometric relationship of each phase trajectory,the problem of trajectory planning is transformed to parameter optimizing,and then auxiliary population-based quantum differential evolution algorithm(AP-QDEA)is applied as a tool to optimize the objective function,and the design parameters of the whole homing trajectory are obtained.The proposed AP-QDEA combines the strengths of differential evolution algorithm(DEA)and quantum evolution algorithm(QEA),and the notion of auxiliary population is introduced into the proposed algorithm to improve the searching precision and speed.The simulation results show that the proposed AP-QDEA is proven its superior in both effectiveness and efficiency by solving a set of benchmark problems,and the multiphase homing scheme can fulfill the requirement of fixed-points and upwind landing in the process of homing which is simple in control and facile in practice as well.
文摘In this study, to explore the radiation protection effects of Lyophyllum Decastes Sing （LDS）, a hot distilled-water extract of LDS was orally administered at a dosage of 250mg/kg every other day for a period of 2 weeks in irradiated mice. An automatic blood cell counter was used to measure white blood cells （lymphocytes, monocyte, and granulocytes） one day before X-ray irradiation, and 3 hours, 12 hours, 24 hours, 3 days, 7 days, 15 days and 30 days after irradiation. The Dunnett test was used to examine statistical significance of differences. The peripheral blood cell counts in the Lyophyllum-administered non-irradiation group revealed an increase in the numbers of ieukocytes, lymphocytes and monocytes. For 2 Gy whole body radiation, a significant statistical difference was found between the X-ray group and the Lyophyllum plus X-ray group in the numbers of leukocytes, lymphocytes and monocytes. The results suggest that Lyophyllum restrains blood cell-count falling after irradiation, which is probably mediated at least in part by hemopoietic function, and NK and LAK activities seems to play a role in preventing secondary irffections associated with irradiation.
文摘Septoria carvi belong to the fungi recently detected on caraway plants in the Polish climatic conditions. In many European countries, this fungus causes significant losses in caraway cultivation and decreases the quality and quantity of the herb material. The aim of the present work was to study the fungus harmfulness towards the sowing materials, stems, leaves and umbels of caraway. The pathogenicity tests according to Koch＇s postulates were carried out for isolates ofS. carvi using post-culture liquids and a water suspension of conidia to inoculate the plants＇ parts. The studies were conducted in the laboratory, the climatic chamber and in field conditions. S. carvi caused disease symptoms in the form of spots and necrosis on all tested plants organs. The infection was followed by natural openings what was shown by scanning electron microscope （SEM） microscopic observations. The efficiency of inoculation increased injuries and anatomical dipping of plant epidermal tissue. The study showed the ability for asymptomatic S. carvi colonization of inoculated plants＇ parts. The performed pathogenicity tests and microscopic observations SEM showed that the tested isolates of S. carvi were occasional pathogens of caraway.
