In this paper we described the construction of a novel dual fluorescent film sensor using fluorescent dye Acridine Orange(AO) as an internal standard and CdTe as a fluorescent probe.Poly-sodium-4-styrenesulfonate(PSS)...In this paper we described the construction of a novel dual fluorescent film sensor using fluorescent dye Acridine Orange(AO) as an internal standard and CdTe as a fluorescent probe.Poly-sodium-4-styrenesulfonate(PSS) and Chitosan(CS) were respectively assembled between AO and CdTe.The constructed self-assembly multilayers,Quartz/APES/PSS/AO/PSS/CS/CdTe,emits dual fluorescence at 600 and 530 nm,respectively.AO is shielded effectively by the assembled PSS and CS and thus its fluorescent intensity I1 doesn′t change with the variation of the analyte concentration,thereby acting as an internal standard to provide reference signal.The ratio of dual fluorescence intensities of the thus constructed film sensor is not affected by the variations of the external environment factors such as excitation intensity and sensor position in the cell holder.A good linear relationship was found between dual fluorescence intensity ratio and DNA concentration over 0-250 ng/L.Quartz/APES/PSS/AO/PSS/CS/CdTe film is capable of an extremely highly sensitive detection for DNA with a detection limit of 6.6 ng/L.The analysis based on dual fluorescent self-assembled multilayers bearing a fluorescent internal standard would greatly improve the detection accuracy.展开更多
A double-sensor probe was used to measure local interfacial parameters of a gas-liquid bubbly flow in a horizontal tube. The parameters included void fraction, interfacial concentration, bubble size distribution, bubb...A double-sensor probe was used to measure local interfacial parameters of a gas-liquid bubbly flow in a horizontal tube. The parameters included void fraction, interfacial concentration, bubble size distribution, bubble frequency and bubble interface velocity. The authors paid special attention to the probe design and construction for minimizing measurement errors. Measures were also taken in the design of sensor ends for preventing corrosions in the flow. This is an effort to improve the current double-sensor probe technique to meet the ever-increasing needs to local parameter measurements in gas-liquid two-phase flows.展开更多
文摘In this paper we described the construction of a novel dual fluorescent film sensor using fluorescent dye Acridine Orange(AO) as an internal standard and CdTe as a fluorescent probe.Poly-sodium-4-styrenesulfonate(PSS) and Chitosan(CS) were respectively assembled between AO and CdTe.The constructed self-assembly multilayers,Quartz/APES/PSS/AO/PSS/CS/CdTe,emits dual fluorescence at 600 and 530 nm,respectively.AO is shielded effectively by the assembled PSS and CS and thus its fluorescent intensity I1 doesn′t change with the variation of the analyte concentration,thereby acting as an internal standard to provide reference signal.The ratio of dual fluorescence intensities of the thus constructed film sensor is not affected by the variations of the external environment factors such as excitation intensity and sensor position in the cell holder.A good linear relationship was found between dual fluorescence intensity ratio and DNA concentration over 0-250 ng/L.Quartz/APES/PSS/AO/PSS/CS/CdTe film is capable of an extremely highly sensitive detection for DNA with a detection limit of 6.6 ng/L.The analysis based on dual fluorescent self-assembled multilayers bearing a fluorescent internal standard would greatly improve the detection accuracy.
基金supported by the National Key R&D Program of China(2021YFE0201400)the National Natural Science Foundation of China(61890940)+5 种基金Chongqing Bayu Scholar Program(DP2020036)the Strategic Priority Research Program of the Chinese Academy of Sciences(XDB30000000)the Program of Shanghai Academic Research Leaders(23XD1420200)China Postdoctoral Science Foundation(2019M661353)the National Postdoctoral Program for Innovative Talents(BX20190072)the State Key Laboratory of Molecular Engineering of Polymers and Fudan University。
基金Supported by the National Natural Science Foundation of China(No.59876032)and the Doctorate Foundation of Xi'an Jiaotong University(DFXJU-17).
文摘A double-sensor probe was used to measure local interfacial parameters of a gas-liquid bubbly flow in a horizontal tube. The parameters included void fraction, interfacial concentration, bubble size distribution, bubble frequency and bubble interface velocity. The authors paid special attention to the probe design and construction for minimizing measurement errors. Measures were also taken in the design of sensor ends for preventing corrosions in the flow. This is an effort to improve the current double-sensor probe technique to meet the ever-increasing needs to local parameter measurements in gas-liquid two-phase flows.
