Objective. To compare the performances of Papanicolaou test (Pap Test) and of a new liquid-based cytology method, DNA-Citoliq. System (DCS), in a high-ri sk population, with histology confirmation. Methods. Paired s...Objective. To compare the performances of Papanicolaou test (Pap Test) and of a new liquid-based cytology method, DNA-Citoliq. System (DCS), in a high-ri sk population, with histology confirmation. Methods. Paired specimens of exfolia ted cervical cells were collected under split-sample protocol. All patients wer e submitted to colposcopy and a biopsy taken when any atypical transformation zo ne was seen. Sensitivity, specificity, positive and negative predictive values, and overall accuracy of both conventional and DCS methods were computed in relat ion to histology. Results. A total of 1,095 patients were analyzed by two cytol ogy methods and, in 425 (38.8%), histologically. There were significantly more adequate samples with DCS (98.63%) than with conventional (89.6%) smears (P < 0.001). ASCUS was diagnosed significantly more with DCS than with conventional P ap (P < 0.001). Conventional Pap misclassified as normal 55.4%(158/285) of case s with either LSIL or HSIL or cancer at histology, whereas DCS misclassified 31. 2%(89/285) of cases (P < 0.001). DCS had a significantly higher sensitivity (70 %and 91.3%) than the conventional Pap (49.8%and 72.8%) to detect both LSIL+ and HSIL+at histology, respectively. On the other hand, specificity of conventi onal smear (88.2%and 85.2%) was significantly higher than DCS (75.4%and 70.9 %) considering both LSIL+and HSIL+at histology, respectively. Conclusions. Th is study confirms the superiority of the liquid-based cytology system DCS to de tect cervical lesions. The rate of adequate DSC slides was significantly higher than with conventional cytology.展开更多
文摘Objective. To compare the performances of Papanicolaou test (Pap Test) and of a new liquid-based cytology method, DNA-Citoliq. System (DCS), in a high-ri sk population, with histology confirmation. Methods. Paired specimens of exfolia ted cervical cells were collected under split-sample protocol. All patients wer e submitted to colposcopy and a biopsy taken when any atypical transformation zo ne was seen. Sensitivity, specificity, positive and negative predictive values, and overall accuracy of both conventional and DCS methods were computed in relat ion to histology. Results. A total of 1,095 patients were analyzed by two cytol ogy methods and, in 425 (38.8%), histologically. There were significantly more adequate samples with DCS (98.63%) than with conventional (89.6%) smears (P < 0.001). ASCUS was diagnosed significantly more with DCS than with conventional P ap (P < 0.001). Conventional Pap misclassified as normal 55.4%(158/285) of case s with either LSIL or HSIL or cancer at histology, whereas DCS misclassified 31. 2%(89/285) of cases (P < 0.001). DCS had a significantly higher sensitivity (70 %and 91.3%) than the conventional Pap (49.8%and 72.8%) to detect both LSIL+ and HSIL+at histology, respectively. On the other hand, specificity of conventi onal smear (88.2%and 85.2%) was significantly higher than DCS (75.4%and 70.9 %) considering both LSIL+and HSIL+at histology, respectively. Conclusions. Th is study confirms the superiority of the liquid-based cytology system DCS to de tect cervical lesions. The rate of adequate DSC slides was significantly higher than with conventional cytology.
