In this paper, we conduct research on differences of Uighur Han Chinese communication etiquette customs and the psychological characteristics under cross-cultural environment. Empathy is the process of cross-cultural ...In this paper, we conduct research on differences of Uighur Han Chinese communication etiquette customs and the psychological characteristics under cross-cultural environment. Empathy is the process of cross-cultural communication subject and adapts to each other and good a better means of communication. In cross-cultural communication, we must improve on the sensitivity of the taboo, taboo customs of different peoples and nations. In this way can eliminate communication barriers and conflict, to achieve smooth with the harmonious language communication. This article will use the theory of intercultural communication with the understanding of the Uygur traditional culture as can dissolve the contradictions and obstacles of the communication. This article from two aspects of the language communication, nonverbal communication in the process of cross-cultural communication problems enumerated and analyzed which will be meaningful.展开更多
Design and development of efficient non-viral gene delivery systems is critical to overcome various barriers for effective intracellular gene delivery.Eight new spermine-based protonatable surfactants were designed,sy...Design and development of efficient non-viral gene delivery systems is critical to overcome various barriers for effective intracellular gene delivery.Eight new spermine-based protonatable surfactants were designed,synthesized and evaluated as non-viral pH-sensitive gene carriers.These carriers formed stable complexes with plasmid DNA at an N/P ratio as low as 2.The sizes of the carrier/pDNA nanoparticles (N/P = 12) were in the range of 90–130 nm,smaller than that of Lipofectamine2000/pDNA nanoparticles.The pDNA nanoparticles of the carriers exhibited substantially increased hemolysis when pH decreased from 7.4 to 5.5.The DNA nanoparticles had low cytotocixity,similar to that of Lipofectamine-2000/pDNA nanoparticles.The pH-sensitive gene carriers with no helper lipids resulted in much higher intracellular transfection and gene expression in U87 cells than Lipofectamine-2000.[N,N′-Bis(oleoylcysteinyl)(β-alanyl-α-lysyl)]-spermine monoamide (SKACO) resulted in the highest luciferase transfection efficiency,more than 400 times higher than that of Lipofectamine-2000,and GFP expression in 71% of transfected U87 cells.SKACO was identified as a promising lead carrier for efficient gene delivery.These spermine-based pH-sensitive amphiphilic carriers have a potential to be further developed as efficient non-viral multifunctional gene delivery systems.展开更多
文摘In this paper, we conduct research on differences of Uighur Han Chinese communication etiquette customs and the psychological characteristics under cross-cultural environment. Empathy is the process of cross-cultural communication subject and adapts to each other and good a better means of communication. In cross-cultural communication, we must improve on the sensitivity of the taboo, taboo customs of different peoples and nations. In this way can eliminate communication barriers and conflict, to achieve smooth with the harmonious language communication. This article will use the theory of intercultural communication with the understanding of the Uygur traditional culture as can dissolve the contradictions and obstacles of the communication. This article from two aspects of the language communication, nonverbal communication in the process of cross-cultural communication problems enumerated and analyzed which will be meaningful.
文摘Design and development of efficient non-viral gene delivery systems is critical to overcome various barriers for effective intracellular gene delivery.Eight new spermine-based protonatable surfactants were designed,synthesized and evaluated as non-viral pH-sensitive gene carriers.These carriers formed stable complexes with plasmid DNA at an N/P ratio as low as 2.The sizes of the carrier/pDNA nanoparticles (N/P = 12) were in the range of 90–130 nm,smaller than that of Lipofectamine2000/pDNA nanoparticles.The pDNA nanoparticles of the carriers exhibited substantially increased hemolysis when pH decreased from 7.4 to 5.5.The DNA nanoparticles had low cytotocixity,similar to that of Lipofectamine-2000/pDNA nanoparticles.The pH-sensitive gene carriers with no helper lipids resulted in much higher intracellular transfection and gene expression in U87 cells than Lipofectamine-2000.[N,N′-Bis(oleoylcysteinyl)(β-alanyl-α-lysyl)]-spermine monoamide (SKACO) resulted in the highest luciferase transfection efficiency,more than 400 times higher than that of Lipofectamine-2000,and GFP expression in 71% of transfected U87 cells.SKACO was identified as a promising lead carrier for efficient gene delivery.These spermine-based pH-sensitive amphiphilic carriers have a potential to be further developed as efficient non-viral multifunctional gene delivery systems.
