传统药物治疗对部分抑郁症患者疗效有限,促使研究者寻找更有效的替代疗法。重复经颅磁刺激(repetitive transcranial magnetic stimulation,rTMS)通过调节大脑特定区域的神经活动,展现出在情感调节和认知功能改善方面的潜力。尽管rTMS...传统药物治疗对部分抑郁症患者疗效有限,促使研究者寻找更有效的替代疗法。重复经颅磁刺激(repetitive transcranial magnetic stimulation,rTMS)通过调节大脑特定区域的神经活动,展现出在情感调节和认知功能改善方面的潜力。尽管rTMS的临床效果已得到证明,但其神经生物学机制仍需进一步研究。脑电图(EEG)微状态分析作为一种高时间分辨率的技术,近年来在研究rTMS对大脑功能的调节机制中发挥了重要作用。展开更多
To promote efficient screening of active angiogenic drugs from traditional medicines, we constructed a humanembryonic kidney-293 cell model using vascular endothelial growth factor （VEGF） gene promoter as the drug t...To promote efficient screening of active angiogenic drugs from traditional medicines, we constructed a humanembryonic kidney-293 cell model using vascular endothelial growth factor （VEGF） gene promoter as the drug target. Inthis model, VEGF gene promoter may regulate the expression of the luciferase reporter gene by responding to thestimulation of drug molecules. This cell model allows rapid and efficient screening of vascular-inducing activecomponents from several drug monomer molecules. Furthermore, we used rat bone marrow mesenchymal stem cells（rMSCs） to conduct a preliminary study on the activity of alantolactone. Using simvastatin as a positive control, weinvestigated the effects of alantolactone on the expression of vascular-related cell marker molecules such as VEGF andα-smooth muscle actin （α-SMA） in rMSCs. According to our results, 0.1, 1, 3 and 5 μM of alantolactone upregulated thetranscriptional luciferase gene activity of VEGF promoter, and a significant difference from that in the control group wasobserved. Among them, 3μM of alantolactone showed the better effect than that of 3 μM of simvastatin （P = 0.036） andother concentrations of alantolactone and simvastatin showed similar effects. Compared with that in the control group,rMSCs induced with 1μM alantolactone for 3 days showed a significant increase in the relative mRNA expressions ofVEGF and α-SMA genes. However, these effect of 5 μM alantolactone were weaker than those of 5 μM simvastatin （P 〈0.05）; rMSCs treated with 1 μM alantolactone for 3 days showed brighter green fluorescence （FITC marker） of α-SMAand VEGF in situ expression than that observed in the control group and similar fluorescence intensity than that ofsimvastatin group in an immunoradiometric assay. The above results demonstrate the reliability of the highly efficientsystem for screening of active drug molecules and confirmed the vascular induction function of alantolactone at the geneand protein levels.展开更多
In view of the rich experiences and new advances in TCM treatment of rheumatoid arthritis, it is very promising to find a further breakthough in the field of traditional Chinese medicine (TCM). Treating the diseas... In view of the rich experiences and new advances in TCM treatment of rheumatoid arthritis, it is very promising to find a further breakthough in the field of traditional Chinese medicine (TCM). Treating the disease by means of differentiation of symptoms and signs is a distinctive feature of TCM, however the difficult and also the major point would be the research of mechanism of its symptomatology. The following is a summary of the advances in the study of symptomatology, especially its epidemiology, the role of immune system and blood rheology made since 1987 when the ARA criteria1 was published.……展开更多
Bu Zhong Yi Qi Tang (补中益气汤Decoction for Reinforcing the Middle-jiao and Replenishing Qi) is an effective formulated recipe commonly used for treating a variety of diseases with the manifestations of kidney-qi... Bu Zhong Yi Qi Tang (补中益气汤Decoction for Reinforcing the Middle-jiao and Replenishing Qi) is an effective formulated recipe commonly used for treating a variety of diseases with the manifestations of kidney-qi deficiency. In my opinion, however, it is also very effective for the conditions of which the symptoms of kidney-qi deficiency is not noticeable, but its mechanism lies in dysfunction of the spleen. The following are some examples.……展开更多
文摘传统药物治疗对部分抑郁症患者疗效有限,促使研究者寻找更有效的替代疗法。重复经颅磁刺激(repetitive transcranial magnetic stimulation,rTMS)通过调节大脑特定区域的神经活动,展现出在情感调节和认知功能改善方面的潜力。尽管rTMS的临床效果已得到证明,但其神经生物学机制仍需进一步研究。脑电图(EEG)微状态分析作为一种高时间分辨率的技术,近年来在研究rTMS对大脑功能的调节机制中发挥了重要作用。
文摘To promote efficient screening of active angiogenic drugs from traditional medicines, we constructed a humanembryonic kidney-293 cell model using vascular endothelial growth factor （VEGF） gene promoter as the drug target. Inthis model, VEGF gene promoter may regulate the expression of the luciferase reporter gene by responding to thestimulation of drug molecules. This cell model allows rapid and efficient screening of vascular-inducing activecomponents from several drug monomer molecules. Furthermore, we used rat bone marrow mesenchymal stem cells（rMSCs） to conduct a preliminary study on the activity of alantolactone. Using simvastatin as a positive control, weinvestigated the effects of alantolactone on the expression of vascular-related cell marker molecules such as VEGF andα-smooth muscle actin （α-SMA） in rMSCs. According to our results, 0.1, 1, 3 and 5 μM of alantolactone upregulated thetranscriptional luciferase gene activity of VEGF promoter, and a significant difference from that in the control group wasobserved. Among them, 3μM of alantolactone showed the better effect than that of 3 μM of simvastatin （P = 0.036） andother concentrations of alantolactone and simvastatin showed similar effects. Compared with that in the control group,rMSCs induced with 1μM alantolactone for 3 days showed a significant increase in the relative mRNA expressions ofVEGF and α-SMA genes. However, these effect of 5 μM alantolactone were weaker than those of 5 μM simvastatin （P 〈0.05）; rMSCs treated with 1 μM alantolactone for 3 days showed brighter green fluorescence （FITC marker） of α-SMAand VEGF in situ expression than that observed in the control group and similar fluorescence intensity than that ofsimvastatin group in an immunoradiometric assay. The above results demonstrate the reliability of the highly efficientsystem for screening of active drug molecules and confirmed the vascular induction function of alantolactone at the geneand protein levels.
文摘 In view of the rich experiences and new advances in TCM treatment of rheumatoid arthritis, it is very promising to find a further breakthough in the field of traditional Chinese medicine (TCM). Treating the disease by means of differentiation of symptoms and signs is a distinctive feature of TCM, however the difficult and also the major point would be the research of mechanism of its symptomatology. The following is a summary of the advances in the study of symptomatology, especially its epidemiology, the role of immune system and blood rheology made since 1987 when the ARA criteria1 was published.……
文摘 Bu Zhong Yi Qi Tang (补中益气汤Decoction for Reinforcing the Middle-jiao and Replenishing Qi) is an effective formulated recipe commonly used for treating a variety of diseases with the manifestations of kidney-qi deficiency. In my opinion, however, it is also very effective for the conditions of which the symptoms of kidney-qi deficiency is not noticeable, but its mechanism lies in dysfunction of the spleen. The following are some examples.……
