Analysis of ISEM, SDS PAGE, Western blot, Northern blot and RT PCR indicated that the soil borne mosaic disease occurred on winter wheat in Yantai district, Shandong Province, was caused by co infection of two differe...Analysis of ISEM, SDS PAGE, Western blot, Northern blot and RT PCR indicated that the soil borne mosaic disease occurred on winter wheat in Yantai district, Shandong Province, was caused by co infection of two different viruses. The filamentous virus was identified to be wheat yellow mosaic virus (WYMV) as it had a coat protein of 33kD, which was serologically related to WYMV, wheat spindle streak mosaic virus(WSSMV) and barley yellow mosaic virus (BaYMV), but not to barley mild mosaic virus(BaMMV) and soil borne wheat mosaic virus(SBWMV). In addition, DNA fragments specifically amplified from its cDNAs template with WYMV primers were identical to those amplified from the homologous template. The rod shaped virus was identified to be a SBWMV related virus as it had a coat protein of 19 kD, which was serologically related to SBWMV and TMV, but not to WYMV, WSSMV, BaYMV, BaMMV and three monoclonal antibodies to SBWMV. Its RNA1 (7.1kb) and RNA2 (3.6 kb) were respectively hybridized by cDNA probes of RNA1(p3H5) and RNA2(pJC2G6) of SBWMV, but no DNA fragments specifically amplified from cDNA template of its RNA with primers of SBWMV RNA 1 and RNA2.展开更多
As a seed transmitted pathogen, pea seed_borne mosaic virus (PSbMV) not only replicates in embryonic cells but can also withstand seed desiccation. To understand the mechanism of PSbMV tolerance to seed desiccation, ...As a seed transmitted pathogen, pea seed_borne mosaic virus (PSbMV) not only replicates in embryonic cells but can also withstand seed desiccation. To understand the mechanism of PSbMV tolerance to seed desiccation, the authors compared the stability of viral coat protein (CP) and the distribution of viral particles in the cotyledon cells of pea (Pisum sativum L.) embryos collected before and after the dehydration process. Before dehydration, when the embryo was fresh and immature, degradation of CP was observed and a predominantly perinuclear distribution of viral particles in the cotyledon cells was evident. After dehydration, when the embryo was dry and mature, degradation of CP did not occur and the perinuclear viral distribution disappeared. Instead, aggregates containing PSbMV CP were found in the cytoplasm. Electron microscopy showed that these aggregates were composed of PSbMV particles. The formation of PSbMV particle aggregates is apparently triggered by seed dehydration and may be favorable to the virus survival in the desiccated embryonic cells.展开更多
基金欧共体资助项目(INCO IC 18-C796-0049)863启动项目+2 种基金中华农业科教基金(97-03-B-6)国家(39600098)和浙江省自然科学基金(396029)浙江省青年科技人才资金(RC9604)
文摘Analysis of ISEM, SDS PAGE, Western blot, Northern blot and RT PCR indicated that the soil borne mosaic disease occurred on winter wheat in Yantai district, Shandong Province, was caused by co infection of two different viruses. The filamentous virus was identified to be wheat yellow mosaic virus (WYMV) as it had a coat protein of 33kD, which was serologically related to WYMV, wheat spindle streak mosaic virus(WSSMV) and barley yellow mosaic virus (BaYMV), but not to barley mild mosaic virus(BaMMV) and soil borne wheat mosaic virus(SBWMV). In addition, DNA fragments specifically amplified from its cDNAs template with WYMV primers were identical to those amplified from the homologous template. The rod shaped virus was identified to be a SBWMV related virus as it had a coat protein of 19 kD, which was serologically related to SBWMV and TMV, but not to WYMV, WSSMV, BaYMV, BaMMV and three monoclonal antibodies to SBWMV. Its RNA1 (7.1kb) and RNA2 (3.6 kb) were respectively hybridized by cDNA probes of RNA1(p3H5) and RNA2(pJC2G6) of SBWMV, but no DNA fragments specifically amplified from cDNA template of its RNA with primers of SBWMV RNA 1 and RNA2.
文摘As a seed transmitted pathogen, pea seed_borne mosaic virus (PSbMV) not only replicates in embryonic cells but can also withstand seed desiccation. To understand the mechanism of PSbMV tolerance to seed desiccation, the authors compared the stability of viral coat protein (CP) and the distribution of viral particles in the cotyledon cells of pea (Pisum sativum L.) embryos collected before and after the dehydration process. Before dehydration, when the embryo was fresh and immature, degradation of CP was observed and a predominantly perinuclear distribution of viral particles in the cotyledon cells was evident. After dehydration, when the embryo was dry and mature, degradation of CP did not occur and the perinuclear viral distribution disappeared. Instead, aggregates containing PSbMV CP were found in the cytoplasm. Electron microscopy showed that these aggregates were composed of PSbMV particles. The formation of PSbMV particle aggregates is apparently triggered by seed dehydration and may be favorable to the virus survival in the desiccated embryonic cells.
