In order to determine an appropriate sampling strategy for the effective conservation of wild soybean (Glycine soja Sieb. et Zucc.) in China, a natural population from Jiangwan Airport in Shanghai was studied for its ...In order to determine an appropriate sampling strategy for the effective conservation of wild soybean (Glycine soja Sieb. et Zucc.) in China, a natural population from Jiangwan Airport in Shanghai was studied for its genetic diversity through the inter-simple sequence repeat (ISSR) marker analysis of a sample set consisting of 100 randomly collected individuals. A relatively large genetic diversity was detected among the samples based on estimation of DNA products amplified from 15 selected ISSR primers, with the similarity coefficient varying from 0.17 to 0.89. The mean expected heterozygosity (He) was 0.171 4 per locus, and Shannon index (1) was 0.271 4. The Principal Coordinate Analysis (PCA) further indicated that genetic diversity of the Jiangwan wild soybean population was not evenly distributed, instead, was presented by a mosaic or clustered distribution pattern. Correlation study between genetic diversity and number of samples demonstrated that genetic diversity increased dramatically with the increase of number of samples within 40 individuals, but the increase became slow and rapidly reached a plateau when more than 40 individuals were included in the analysis. It is concluded that (i) a sample set of approximately 35-45 individuals should be included to represent possibly high genetic diversity when conservation of a wild soybean population ex situ is undertaken; and (ii) collection of wild soybean samples should be spread out as wide as possible within a population, and a certain distance should be kept as intervals among individuals for sampling.展开更多
In order to evaluate the genetic relationships between glutinous sorghum germplasms, 25 pair of SSR markers were used to analyze the genetic diversity of29 varieties. The result showed that a total of 59 alleles were ...In order to evaluate the genetic relationships between glutinous sorghum germplasms, 25 pair of SSR markers were used to analyze the genetic diversity of29 varieties. The result showed that a total of 59 alleles were identified with an average of 2.28 alleles per locus. The average effective number of alleles（NE） was1.81.The average polymorphism information content（PIC） varied widely from 0.17 to0.62 with an average value of 0.34.The similarity coefficients of the 29 glutinous sorghum germplasms ranged from 0.203 to 0.949 with an average value of 0.593.Clustering analysis with UPGMA showed that the varieties could be divided into two groups at the genetic similarity coefficient of 0.475.The varieties from different geographic originswere clustered into one subclass, and the varieties with similar agronomic traits were clustered into one subclass, which indicated that the genetic relationships among materials was not in accordance with their geographical origins,and the glutinous sorghum germplasm resources showed abundant genetic diversity in the study.展开更多
[Objective]The aim was to analyze genetic diversity of SSR markers in Hordeum vulgare L.in Qinghai Province and lay a foundation for screening and protecting some excellent H.vulgare cultivars.[Method]SSR markers were...[Objective]The aim was to analyze genetic diversity of SSR markers in Hordeum vulgare L.in Qinghai Province and lay a foundation for screening and protecting some excellent H.vulgare cultivars.[Method]SSR markers were used to evaluate the genetic diversity of 42 cultivated H.vulgare from Qinghai Province.[Result]42 H.vulgare showed polymorphism in 7 SSR markers locus.A total of 24 alleles were identified,and the number of alleles per locus ranged from 1 to 6,with an average of 3.0.According to SSR markers polymorphism,42 H.vulgare could be divided into 4 groups,namely I,II,III and IV.[Result]The study indicated that cultivated H.vulgare from Qinghai Province is rich in genetic diversity,which will provide reference for selecting parent of H.vulgare breeding.展开更多
Twenty-two nitrate nonutilizing （nit） mutants were recovered from five wild-type isolates of Fusarium graminearum and fifty nit mutants were recovered from three JS399-19-resistant mutants of F. graminearum cultured...Twenty-two nitrate nonutilizing （nit） mutants were recovered from five wild-type isolates of Fusarium graminearum and fifty nit mutants were recovered from three JS399-19-resistant mutants of F. graminearum cultured on MMC medium. Some biological properties were compared between nit mutants and their parental isolates. The results showed that there were no significant differences in growth rate, cultural characters or pathogenicity between JS399-19-resistant nit mutants and their parental isolates. But the conidial production and the sexual reproduction ability changed to some extent. There was no cross resistance toward chlorate and JS399-19 in F. graminearum and the resistance could be stable through 20-time subcultures. Therefore, the nit could be used as a genetic marker for studying the genetics of JS399-19 resistance in E graminearum, which was used to study JS399-19 resistance transferability in hyphal fusion. Resistance in JS399-19 could not be transferred by hyphal fusion or could be transferred with low chance between two compatible isolates, which would delay the development of JS399-19 resistance in the field.展开更多
[Objective] This study aimed to analyze the genetic characteristics of Salvia miltiorrhiza by using cpSSR （chloroplast microsatellites） molecular marker method. [Method] Twelve pairs of SSR primers with ideal cpSSR ...[Objective] This study aimed to analyze the genetic characteristics of Salvia miltiorrhiza by using cpSSR （chloroplast microsatellites） molecular marker method. [Method] Twelve pairs of SSR primers with ideal cpSSR amplification result, good repeatability and clear amplification bands were selected for cpSSR detection and analysis of S. miltiorrhiza samples from 31 sampling locations in 25 counties of 8 provinces in China. [Result] S. miltiorrhiza shows an overall medium level in the cytoplasmic inheritance （cpSSR） and varying degrees of differences in the regions. Based on Shannon’s Information index （I） and Nei’s genetic diversity index, the cytoplasmic genetic diversity in different provinces shows a decreasing order of Shanxi, Henan, Shandong, Hebei, Sichuan, Jiangsu, Shaanxi, Anhui. Genetic variations of S. miltiorrhiza in eight provinces were found mainly among populations. Gene flow among populations with in a province is less than that among different provinces. [Conclusion] Comprehensive analysis shows that S. miltiorrhiza cultivated in genuine producing areas and traditional main producing areas are mainly introduced from the local before introduction of some foreign germplasm resources during cultivation. The exchange of S. miltiorrhiza germplasm resources exists very early among genuine producing areas such as Sichuan, Shandong and Henan, while S. miltiorrhiza germplasm resources in new producing areas are mainly introduced from genuine producing areas. However, no geographical correlation was observed in the genetic differentiation, which further reveals that there is a wide range of genetic exchange among S. miltiorrhiza germplasm resources in China, resulting from the artificial introduction from allopatry.展开更多
[ Objective ] This study was to analyze the genetic polymorphism in GDF8 Region of HU sheep. [Method] Four microsatellite loci including BMS1591, TEXAN-2, FCB128 and BM81124 mapped on GDF8 region of chromosome No. 2 o...[ Objective ] This study was to analyze the genetic polymorphism in GDF8 Region of HU sheep. [Method] Four microsatellite loci including BMS1591, TEXAN-2, FCB128 and BM81124 mapped on GDF8 region of chromosome No. 2 of sheep that may be correlated with growth performance were chosen to detect the molecular genetics foundation of growth performance of Hu sheep. [ Result] Four microsatellite loci detected were high in heterozygosity, more in effective alleles number and rich in polymorphic information, all the three indices passed through the high polymorphic level （PIC 〉0.5）. [ Conclusion ] The four microsatellite loci detected could be used to estimate the genetic polymorphism of growth performance of Hu sheep.展开更多
The genetic diversity among 32 accessions of Hordeum bogdanii Wilensky native to Xinjiang, China, was evaluated by 22 STS_PCR primer sets derived from RFLP clones of the wheat ( Triticum aestivum L.) or barley ...The genetic diversity among 32 accessions of Hordeum bogdanii Wilensky native to Xinjiang, China, was evaluated by 22 STS_PCR primer sets derived from RFLP clones of the wheat ( Triticum aestivum L.) or barley ( Hordeum vulgare L.) mapping. Out of the 22 STS_PCR markers, only three markers gave products which did not generate polymorphic bands upon digestion with Hin fⅠ, Hha Ⅰ, Hae Ⅲ and Rsa Ⅰ, while 19 out of 22 markers (86.4%) and 46 out of 88 marker/enzyme combinations (52.3%) revealed polymorphisms. Among the 32 H. bogdanii accessions, a total of 315 bands were observed in 88 STS_PCR marker/enzyme combinations, with 3.6 bands each. One hundred and twenty_three out of 315 bands (39.0%) were polymorphic, among which 1 to 6 polymorphic bands were generated by each polymorphic marker/enzyme combination. The STS_PCR_based genetic diversity index ( GD ) among 32 H. bogdanii accessions ranged between 0.078 to 0.352, with a mean of 0.198. Based on the GD matrix, a dendrogram showing the genetic relationships between accessions was constructed using the unweighted pair_group method with arithmetic average (UPGMA). Results showed that all 32 accessions could be distinguished by STS_PCR markers. The accessions originated from the same region were distributed within different groups or subgroups. This study indicates that the genetic diversity of H. bogdanii is not closely correlated with the geographical distribution.展开更多
文摘In order to determine an appropriate sampling strategy for the effective conservation of wild soybean (Glycine soja Sieb. et Zucc.) in China, a natural population from Jiangwan Airport in Shanghai was studied for its genetic diversity through the inter-simple sequence repeat (ISSR) marker analysis of a sample set consisting of 100 randomly collected individuals. A relatively large genetic diversity was detected among the samples based on estimation of DNA products amplified from 15 selected ISSR primers, with the similarity coefficient varying from 0.17 to 0.89. The mean expected heterozygosity (He) was 0.171 4 per locus, and Shannon index (1) was 0.271 4. The Principal Coordinate Analysis (PCA) further indicated that genetic diversity of the Jiangwan wild soybean population was not evenly distributed, instead, was presented by a mosaic or clustered distribution pattern. Correlation study between genetic diversity and number of samples demonstrated that genetic diversity increased dramatically with the increase of number of samples within 40 individuals, but the increase became slow and rapidly reached a plateau when more than 40 individuals were included in the analysis. It is concluded that (i) a sample set of approximately 35-45 individuals should be included to represent possibly high genetic diversity when conservation of a wild soybean population ex situ is undertaken; and (ii) collection of wild soybean samples should be spread out as wide as possible within a population, and a certain distance should be kept as intervals among individuals for sampling.
基金Supported by the Earmarked Fund for Modern Agro-industry Technology Research System(CARS-06-01-05)Secondary Subproject of the National Key Technology Research and Development Program during the 12~th Five-year Plan Period of China(2014BAD07B02-2-2)Key Project of Crop Breeding of Sichuang Province(2011NZ0098-6)~~
文摘In order to evaluate the genetic relationships between glutinous sorghum germplasms, 25 pair of SSR markers were used to analyze the genetic diversity of29 varieties. The result showed that a total of 59 alleles were identified with an average of 2.28 alleles per locus. The average effective number of alleles（NE） was1.81.The average polymorphism information content（PIC） varied widely from 0.17 to0.62 with an average value of 0.34.The similarity coefficients of the 29 glutinous sorghum germplasms ranged from 0.203 to 0.949 with an average value of 0.593.Clustering analysis with UPGMA showed that the varieties could be divided into two groups at the genetic similarity coefficient of 0.475.The varieties from different geographic originswere clustered into one subclass, and the varieties with similar agronomic traits were clustered into one subclass, which indicated that the genetic relationships among materials was not in accordance with their geographical origins,and the glutinous sorghum germplasm resources showed abundant genetic diversity in the study.
基金Supported by National Science and Technology Support Projects(2007BAD64B01)~~
文摘[Objective]The aim was to analyze genetic diversity of SSR markers in Hordeum vulgare L.in Qinghai Province and lay a foundation for screening and protecting some excellent H.vulgare cultivars.[Method]SSR markers were used to evaluate the genetic diversity of 42 cultivated H.vulgare from Qinghai Province.[Result]42 H.vulgare showed polymorphism in 7 SSR markers locus.A total of 24 alleles were identified,and the number of alleles per locus ranged from 1 to 6,with an average of 3.0.According to SSR markers polymorphism,42 H.vulgare could be divided into 4 groups,namely I,II,III and IV.[Result]The study indicated that cultivated H.vulgare from Qinghai Province is rich in genetic diversity,which will provide reference for selecting parent of H.vulgare breeding.
基金This work was supported by the State "973" Programs from the Ministry of Science and Technology of China (No. 2006CB101900)Technology and the Project (No. 20050307028)+3 种基金from the Ministry of Education of China, the National Natural Science Foundation of China (No. 30671048 & No. 30671384)Jiangsu Provincial Program for Tackling Key Problems of Science and Technology (No. BG2006328)the Key Technology R & D program from the Ministry of Science and Technology of China (No. 2006BAE01A04-08)the state "863" programs from the Ministry of Science and Technology of China (No. 2006AA10A211).
文摘Twenty-two nitrate nonutilizing （nit） mutants were recovered from five wild-type isolates of Fusarium graminearum and fifty nit mutants were recovered from three JS399-19-resistant mutants of F. graminearum cultured on MMC medium. Some biological properties were compared between nit mutants and their parental isolates. The results showed that there were no significant differences in growth rate, cultural characters or pathogenicity between JS399-19-resistant nit mutants and their parental isolates. But the conidial production and the sexual reproduction ability changed to some extent. There was no cross resistance toward chlorate and JS399-19 in F. graminearum and the resistance could be stable through 20-time subcultures. Therefore, the nit could be used as a genetic marker for studying the genetics of JS399-19 resistance in E graminearum, which was used to study JS399-19 resistance transferability in hyphal fusion. Resistance in JS399-19 could not be transferred by hyphal fusion or could be transferred with low chance between two compatible isolates, which would delay the development of JS399-19 resistance in the field.
基金Supported by the "Eleventh Five-Year" National Science and Technology Support Program (2006BAI09B03-4)Project from Sichuan Administration of Traditional Chinese Medicine (2010-06)+1 种基金National Natural Science Foundation of China (81173493 81001610)~~
文摘[Objective] This study aimed to analyze the genetic characteristics of Salvia miltiorrhiza by using cpSSR （chloroplast microsatellites） molecular marker method. [Method] Twelve pairs of SSR primers with ideal cpSSR amplification result, good repeatability and clear amplification bands were selected for cpSSR detection and analysis of S. miltiorrhiza samples from 31 sampling locations in 25 counties of 8 provinces in China. [Result] S. miltiorrhiza shows an overall medium level in the cytoplasmic inheritance （cpSSR） and varying degrees of differences in the regions. Based on Shannon’s Information index （I） and Nei’s genetic diversity index, the cytoplasmic genetic diversity in different provinces shows a decreasing order of Shanxi, Henan, Shandong, Hebei, Sichuan, Jiangsu, Shaanxi, Anhui. Genetic variations of S. miltiorrhiza in eight provinces were found mainly among populations. Gene flow among populations with in a province is less than that among different provinces. [Conclusion] Comprehensive analysis shows that S. miltiorrhiza cultivated in genuine producing areas and traditional main producing areas are mainly introduced from the local before introduction of some foreign germplasm resources during cultivation. The exchange of S. miltiorrhiza germplasm resources exists very early among genuine producing areas such as Sichuan, Shandong and Henan, while S. miltiorrhiza germplasm resources in new producing areas are mainly introduced from genuine producing areas. However, no geographical correlation was observed in the genetic differentiation, which further reveals that there is a wide range of genetic exchange among S. miltiorrhiza germplasm resources in China, resulting from the artificial introduction from allopatry.
基金Supported by the State Scientific Basic Research Platform Program( No. 2005DKA21101)China Postdoctoral Science Foundation(No. 20080430470)+7 种基金Natural Science Foundation of Jiangsu Prov-ince of China (BK2007556)the National high-tech R&D program(863 program)(No.2006AA10Z198)Key Projects in the NationalScience &Technology Pillar Program during the Eleventh Five-Year Plan Period(No.2006BAD13B08 )Support Foundation of Chinaduring the 11th Five-Year Plan Period (No. 2008BADB2B04)BasicNatural Science Foundation for Colleges and Universities in JiangsuProvince (NK051039)Jiangsu Government Scholarship for Over-seas Studies ProjectQing Lan Project of Colleges and UniversitiesJiangsu Provincethe New Century Talent Project of Yangzhou University in China~~
文摘[ Objective ] This study was to analyze the genetic polymorphism in GDF8 Region of HU sheep. [Method] Four microsatellite loci including BMS1591, TEXAN-2, FCB128 and BM81124 mapped on GDF8 region of chromosome No. 2 of sheep that may be correlated with growth performance were chosen to detect the molecular genetics foundation of growth performance of Hu sheep. [ Result] Four microsatellite loci detected were high in heterozygosity, more in effective alleles number and rich in polymorphic information, all the three indices passed through the high polymorphic level （PIC 〉0.5）. [ Conclusion ] The four microsatellite loci detected could be used to estimate the genetic polymorphism of growth performance of Hu sheep.
文摘The genetic diversity among 32 accessions of Hordeum bogdanii Wilensky native to Xinjiang, China, was evaluated by 22 STS_PCR primer sets derived from RFLP clones of the wheat ( Triticum aestivum L.) or barley ( Hordeum vulgare L.) mapping. Out of the 22 STS_PCR markers, only three markers gave products which did not generate polymorphic bands upon digestion with Hin fⅠ, Hha Ⅰ, Hae Ⅲ and Rsa Ⅰ, while 19 out of 22 markers (86.4%) and 46 out of 88 marker/enzyme combinations (52.3%) revealed polymorphisms. Among the 32 H. bogdanii accessions, a total of 315 bands were observed in 88 STS_PCR marker/enzyme combinations, with 3.6 bands each. One hundred and twenty_three out of 315 bands (39.0%) were polymorphic, among which 1 to 6 polymorphic bands were generated by each polymorphic marker/enzyme combination. The STS_PCR_based genetic diversity index ( GD ) among 32 H. bogdanii accessions ranged between 0.078 to 0.352, with a mean of 0.198. Based on the GD matrix, a dendrogram showing the genetic relationships between accessions was constructed using the unweighted pair_group method with arithmetic average (UPGMA). Results showed that all 32 accessions could be distinguished by STS_PCR markers. The accessions originated from the same region were distributed within different groups or subgroups. This study indicates that the genetic diversity of H. bogdanii is not closely correlated with the geographical distribution.
