重塑民众情感:王世贞《严嵩传》的多重书写及情感表达

传记有塑造民众情感的作用,严嵩形象从其个人的“自赞”“像赞”到王世贞的传记书写,再到民间叙事、国家叙事,传主形象“翻转”的背后寓含着书写者情感表达的转向。严嵩传文本众多,书写过程复杂,王世贞较早为其撰成“权奸”传,并通过志传、诗传、史传等多重书写,从多个层面助推其入《明史》“奸臣传”。严嵩位居首辅,主政多年,与诸多文人名士交游,获得了大量文化资本,即使在失宠致仕情况下,将其列入“奸臣传”也非易事。为将其塑造成为“权奸”,王世贞撰写五篇严嵩传和一首诗传,或据历史事实“实录”,或发抒内心情感,特别是借助《鸣凤记》等传奇文本,利用声音和表演所特有的认同性和顺从性,将个人“攻击—对立”型厌恶情感上升为普通大众所能接受的认知和情感,从“民间”文化形态使其奸佞形象获得社会广泛认同,广大民众也积极参与到严嵩奸臣形象建构当中。严嵩形象凝聚着王世贞个人及民众、国家的共同情感以及民族文化记忆。

Genetic Structure of Malus sieversii Population from Xinjiang,China,Revealed by SSR Markers

One hundred and nine Malus sieversii accessions from four geographical populations located at Kuerdening in Mohe town, Gongliu County, Jiaowutuohai, in Xinyuan County, Daxigou in Houcheng County of Ily State, and Baerluke Mountain in Yumin County of Tacheng State, Xinjiang Uygur Autonomous Region of China were studied by SSR markers. The purpose of the study was to determine the genetic structure and diversity in these eco-geographical populations with eight pair SSR primers of apple. The results indicated that： an average of 16 bands was detected in the four populations. The percentage of polymorphic bands in Gongliu population （89.06%） was the highest in the four populations. The average Nei＇s gene diversity index was 0.257 for all the loci. Totally, 128 polymorphic loci were detected and the percentage of polymorphic loci （P） was 100%, 88.28%, 84.83%, 87.50%, and 87.12%, respectively, at the species level and Gongliu, Xinyuan, Huocheng, and Yumin population levels. The Nei＇s gene diversity index （H = 0.2619） and Shannon＇s information index （1 = 0.4082） in the species level were higher than in the population level. The Nei＇s gene diversity index and Shannon＇s information index in the four populations were Gongliu 〉 Huocheng 〉 Xinyuan 〉 Yumin. Gongliu population and Xinyuan population were the highest in genetic identity and the closest in genetic distance. Gene flow between the populations was 7.265 based on genetic differentiation coefficient （GST = 0.064）. The UPGMA cluster analysis indicated that the genetic relationships between the Gongliu and Xinyuan population were the closest, and the Yumin population were the farthest with the other three populations. The UPGMA cluster analysis indicated that the four geographical populations located in Gongliu, Xinyuan, Huocheng, and Yumin were relatively independent populations. Concurrently, there was also mild gene exchange between the populations. On the basis of the study of population genetic structure and the highest genetic diversity, Gongliu population should be given a high priority consideration in Malus sieversii population＇s in situ germplasm conservation.

Population Genetic Structure in Apricot (Prunus armeniaca L.) Cultivars Revealed by Fluorescent-AFLP Markers in Southern Xinjiang,China

Population-wide genetic structure was studied using fluorescent-AFLP markers on 85 apricot （Prunus armeniaca L.） cultivars collected from Kuche, Kashi, Hetian in the Tarim Basin, southern Xinjiang Uygur Autonomous Region of China. The purpose of this study was to determine the genetic structure and genotypic diversity among the different eco-geographical populations. Based on the results from this study, 8 pairs of fluorescent-AFLP primers showed clear electrophoregram and high polymorphism amongst the 64 pairs of EcoR Ⅰ/Mse Ⅰ （Mse Ⅰ - a FAM fluorescent marked primer） primers screened. There was a significant polymorphic difference for the same primer pair in different populations and for the same population with different primer pairs. The percentage of polymorphic loci （P） at species level was higher than Kuche, Hetian, Kashi population levels, respectively. The Nei＇s gene diversity index （H） and Shannon＇s information index （I） at species level were higher than those of Kuche, Hetian, and Kashi at population level, respectively. H and I of Kuche population were the highest amongst the three populations. Apricot population genetic diversity was found mainly within the population, Genetic differentiation coefficient between populations （GST） was 0.0882. Gene flow Nm between the populations was 5.1689. Population genetic identity was between 0.9772-0.9811 and genetic distance was between 0.0191-0.0232. These results further indicated that the similarity between populations was higher and the genetic distance between populations was smaller. The UPGMA cluster analysis indicates that the geographical populations at Kuche, Kashi, Hetian were relatively independent Mendelian populations. Concurrently, there was also partial gene exchange between the populations. All the evidences indicated that the genetic diversity in Kuche population was the highest, suggesting that it could be a transition population from wild apricot to cultivated apricot. There were abundant genetic diversities in apricot cultivar populations in southern Xinjiang, China, which provide promising germplasm for further breeding and theoretical basis for biodiversity conservation and utilization for apricot population in this area.

RFLP Detection of Genetic Variation of Maize Inbred Lines

Genetic similarities of 13 inbred lines of maize (Zea mays L.) were analyzed by restriction fragment length polymorphisms (RFLPs). The objectives of the study were to detect genetic similarities among 13 inbreds and to assign them to heterotic groups. By means of 24 probe_enzyme combinations (PECs) selected for locus specificity, clear patterns and reproducibility, 85 alleles were found with an average of 3.3 alleles per locus. The allelic frequency data were used to estimate genetic similarities among lines, and as a result the diversity index of 0.499 was obtained. Genetic similarities between the pairs of 13 lines ranged from 0.523 up to 0.802 with an average of 0.649. The UPGMA clustering algorithm analysis classified the 13 lines into five groups, which generally corresponded to known maize heterotic groups based on pedigree information. The authors concluded that RFLP_based markers could be used for investigating genetic relationships between maize inbred lines and assigning them to heterotic groups, but it seemed that a large number of PECs were needed to obtain reliable estimates of genetic similarity.

Intestinal microflora molecular markers of spleen-deficient rats and evaluation of traditional Chinese drugs

AIM:To find a rapid and efficient analysis method of gastrointestinal microflora in Pi-deficient(spleen-deficient) rats and to evaluate traditional Chinese drugs.METHODS:Enterobacterial repetitive intergenic consensus-PCR(ERIC-PCR) based assay was performed to examine changes of intestinal microflora in two Pi-deficienct animal models and to evaluate the efficacy of four traditional Chinese drugs as well as a probiotic recipe and another therapy in Pi-deficient rats.RESULTS:A molecular marker was identified for Pi-deficiency in rats.The pharmacodynamic evaluation system,including identified molecular markers(net integral area and abundance of DNA bands),Shannon's index for diversity of intestinal microflora,and Sorenson's pairwise similarity coefficient,was established.The four major clinical recipes of traditional Chinese drugs for Pi-deficiency in rats,especially at their medium dose(equivalence to the clinical dose),produced more pronounced recovery activities in Pi-deficient rats,while higher doses of these recipes did not show a better therapeutic effect but some toxic effects such as perturbation deterioration of intestinal microflora.CONCLUSION:Both fingerprint analysis and identified marker can show Pi-deficiency in rats and its difference after treatment.The identified molecular marker may be applied in screening for the active compounds both in relative traditional Chinese drugs and in pharmacodynamic study of Pi-deficiency in rats.

双线并行 思维整合——人物传记任务群教学举隅

在人物传记任务群教学实践活动中,可以学写人物传记与发展思维能力双线并行为目标,以怎样写好人物传记为驱动,以高阶认知策略带动低阶认知策略为思维导向,开展系列学习实践与探究活动。在建构和运用人物传记概念性知识的同时发展高阶思维,达成直觉思维、形象思维、逻辑思维、辩证思维和创造思维的全面整合与落地。

The Role of Memory and Tradition in the Construction of Identity in the Nineteenth-Century Bahamas

Tradition has been defined as practices brought forward from the past into the present. In the context of enslavement and the cultural dislocation that accompanied it, memory became critical in the recreation of tradition. Individual memory contributed to the practices that created new traditions to be carried forward by subsequent generations. Archaeological evidence from Clifton Plantation, Bahamas, illustrates how memory and tradition shaped the identities of both enslaver and enslaved, and influenced the construction of an African Bahamian identity in the early nineteenth-century. In their consumer selections, the enslaved people of Clifton were constructing artifact assemblages that reflected their memories of their traditional cultural background. In the process they were creating an Afro-Bahamian aesthetic that would become a tradition for future generations of Bahamians. While the goods were not of their own manufacture, the choices were theirs from the selection available to them. At the same time, those of British heritage were signaling their British identity through their consumer choices. However, Clifton was unusual in being owned by a reformer who sought to ameliorate the conditions of slavery. The paper also briefly discusses whether these concepts are useful in understanding the material culture of enslaved Africans at other sites.

Application of AFLP markers for population genetic study on half-smooth tongue sole Cynoglossus semilaevis

The genetic diversity of wild and hatchery populations of half-smooth tongue sole Cynoglossus semilaevis, based on observation of amplified fragment length polymorphism (AFLP) was described. Two hundred individuals from four wild populations, Laizhou (LZ), Weihai (WH), Qingdao (QD), Rizhao (RZ), and one hatchery population, Mingbo (MB), were screened using eight different AFLP primer combinations. A total of 384 loci were screened in the five studied populations. 48.4%, 51.3%, 50.7%, 49.3% and 45.8% of these loci were polymorphic among the individuals tested in the LZ, WH, QD, RZ and MB populations, respectively. The number of polymorphic loci detected by single primer combinations ranged from 17 to 35. The average heterozygosity of the LZ, WH, QD, RZ and MB populations was 0.072, 0.093, 0.092, 0.090 and 0.063, respectively. The WH population showed the highest genetic diversity in terms of total number of AFLP bands, total number of polymorphic bands, average heterozygosity and percentage of low frequency (0-0.2) polymorphic loci among all the populations, while the LZ population was the lowest among the wild populations. Compared with the wild populations, the hatchery population showed a low genetic viability.

Genetic Diversity of Cameroon Native Goat Populations Revealed by Caprine Microsatellites

A total of seventeen caprine microsatellite markers were used on 169 goats to investigate genetic diversity of eight Cameroon native goat populations and to assess genetic differentiation with the East African small goat. All microsatellites showed a high polymorphic content （PIC） of more than 0.5 in almost all ecotypes. These microsatellite markers were useful for native goat ecotypes variability assessment. Expected heterozygosity of all ecotypes was above 0.5, varying from 0.2 to 0.7. Only goats from Eastern rainforest ecotype deviated from Hardy-Weinberg equilibrium test （P 〉 0.001）. Although geographic distribution was a good indication of differentiation, there appeared a tendency of genetic exchange between various ecotypes in Cameroon native goats.

Genetic Diversity Analysis of Cultured Populations of Jade Perch （Scortum bacoo） in China Using AFLP Markers

Genetic diversity of three cultured populations of jade perch （Scortum bacoo） are studied using amplified fragment length polymorphism （AFLP） markers, which is Guangzhou（GZ） population, Foshan （FS） population and Qingdao（QD） population. Nine primer combinations were used and 385 fragments were detected. Among the 385 fragments, 80 bands （20.78%） were polymorphic. And it can be speculated that the genetic diversity of the three cultured populations of jade perch was very poor according to the gene genetic diversity among populations （Ht）, the gene genetic diversity within populations （Hs） and Shannon-Weiner index （I）. From gene differentiation （Gst）, genetic distance （D）, genetic similarity （5）, and UPGMA analysis, it is found that the relationship among the three populations is very closed, and the difference in genetic diversity among the three populations is not significant. Further clustering relationships of the jade perch cultured populations also are correlated to historical-breeding observations and genetic relationships. The GZ population clustered together with the QD population first, then with FS population.

Genetic Diversity of Toona ciliata Populations based on SSR Markers

In order to provide a theoretical basis for the protection and development of T. ciliata germplasm resources, we studied the genetic diversity of T. ciliata by using SSR(Simple Sequence Repeat) primers to evaluate the genetic diversity of 192 T. ciliata germplasm samples from 24 populations of 5 provinces. Data Formater, Popgene, NTSYS, TFPGA and other software were used for genetic data conversion, genetic parameter estimation, dendrogram construction and genetic variation analysis. The results showed that: 1) a total of 17 alleles(Na) were detected in seven pairs of primers, with an average of 2.260 for each primer. Among them, the highest numbers of alleles(4) were detected in primers S11 and S422.The mean value of Nei’s genetic diversity index(H) was 0.4909, the mean value of Shannon information index(I) was 0.7321, and the mean value of polymorphic information content(PIC) was 0.5182. The mean expected heterozygosity(He) and observed heterozygosity(Ho) were 0.1055 and 0.4956, respectively. The Nei’s genetic distances of the populations ranged between 0.0002 and 2.6346, and the mean was 0.5477. The average genetic diversity level(H=0.1044) of the 24 populations was lower than that of the species(H=0.4909). 2) The genetic differentiation coefficients(Fst) varied from 0.2374 to 0.9148, with an average value of 0.7727. The mean of population gene flow(Nm) was 0.0735, indicating a low level of genetic exchange between populations, and suggesting that the genetic variation mainly came from within populations. 3) With the UPGMA method, the 24 populations were clustered into 3 groups at Nei’s genetic identity(0.99): the populations from Guizhou Province and Guangxi Zhuang Autonomous Region were clustered into one group, the populations from Hunan Province were in another group, and the populations from Hubei Province were in the third group. The Mantel test analysis showed a significant correlation between Nei’s genetic distance and geographic distance(r=0.6318, P=0.009<0.05). The genetic diversity of the 24 populations of T. ciliata was at a low level. Geographic isolation was the main reason for genetic differentiation among T. ciliata provenances. In the protection of germplasm resources of T. ciliata, emphasis should be placed on breeding genetic resources from the populations with higher genetic diversity(P14, for example). As for the populations with low genetic diversity, an ex-situ protection strategy as well as ecological and timber objectives, should be taken into account to maximize the conservation and utilization of the diversity of T. ciliata.