Recently, chitosan nanoparticles have been an attractive biomaterial for their unique properties. Chitosan nanoparticles are mainly used as drug delivers. However, they are seldom used in wound healing after severe bl...Recently, chitosan nanoparticles have been an attractive biomaterial for their unique properties. Chitosan nanoparticles are mainly used as drug delivers. However, they are seldom used in wound healing after severe bleeding. In the present paper, chitosan nanoparticles with an average size of 60.2 nm were prepared through ionic gelation and characterized by X-ray diffraction and FT-infrared spectrometry. A new animal model of severe bleeding was made to evaluate its effects on wound healing besides hemostasis. The results showed that it can achieve well hemostatic effects and also induce wound healing after severe bleeding. Blood cells can be absorbed by chitosan nanoparticles and the bleeding is treated. Furthermore, the chitosan nanoparticles sustains longer than other materials in wound healing. At the 15th day after operation, the chitosan nanoparticles with the size of 58.3 nm can still be seen in the new-regenerated hepatic tissue. It can induce the formation of a clear granulation around the wound site. Through histological observation, we find out that chitosan-TPP nanoparticles can induce wound healing through migration of the inflammatory cells and synthesis of collagen. So, chitosan nanoparticles show us both hemostasis and the ability of wound healing for parenchymal organs.展开更多
Wound healing has invariably been a fundamental health concern,demanding manpower and materials and causing financial burdens.In this research,inspired by the hemostatic function of platelets,we proposed a novel bioni...Wound healing has invariably been a fundamental health concern,demanding manpower and materials and causing financial burdens.In this research,inspired by the hemostatic function of platelets,we proposed a novel bionic hydrogel by covalent amidation crosslinking natural platelet and alginate for wound healing.With the natural functional groups,the platelet-derived hydrogel exhibited outstanding biocompatibility and blood compatibility.By changing the addition ratio of platelets to alginates,the mechanical properties of the achieved hydrogel were variable to cater to different wound environments.Furthermore,silver nanoparticles could be loaded into the void space of the hydrogel which endowed the composites with superior anti-infective properties.We have demonstrated that the bio-inspired platelet hydrogel could promote hemostasis of acute tissue damage,prevent bacterial proliferation,and promote angiogenesis,collagen deposition,and granulation tissue formation in wound healing.These features signify the potential values of the bio-inspired platelet hydrogel in clinical applications.展开更多
文摘Recently, chitosan nanoparticles have been an attractive biomaterial for their unique properties. Chitosan nanoparticles are mainly used as drug delivers. However, they are seldom used in wound healing after severe bleeding. In the present paper, chitosan nanoparticles with an average size of 60.2 nm were prepared through ionic gelation and characterized by X-ray diffraction and FT-infrared spectrometry. A new animal model of severe bleeding was made to evaluate its effects on wound healing besides hemostasis. The results showed that it can achieve well hemostatic effects and also induce wound healing after severe bleeding. Blood cells can be absorbed by chitosan nanoparticles and the bleeding is treated. Furthermore, the chitosan nanoparticles sustains longer than other materials in wound healing. At the 15th day after operation, the chitosan nanoparticles with the size of 58.3 nm can still be seen in the new-regenerated hepatic tissue. It can induce the formation of a clear granulation around the wound site. Through histological observation, we find out that chitosan-TPP nanoparticles can induce wound healing through migration of the inflammatory cells and synthesis of collagen. So, chitosan nanoparticles show us both hemostasis and the ability of wound healing for parenchymal organs.
基金supported by the National Key Research and Development Program of China(2020YFA0908200)the National Natural Science Foundation of China(52073060 and 22002061)+1 种基金the Natural Science Foundation of Jiangsu(BK20200551)the Shenzhen Fundamental Research Program(JCYJ20190813152616459 and JCYJ20210324133214038)。
文摘Wound healing has invariably been a fundamental health concern,demanding manpower and materials and causing financial burdens.In this research,inspired by the hemostatic function of platelets,we proposed a novel bionic hydrogel by covalent amidation crosslinking natural platelet and alginate for wound healing.With the natural functional groups,the platelet-derived hydrogel exhibited outstanding biocompatibility and blood compatibility.By changing the addition ratio of platelets to alginates,the mechanical properties of the achieved hydrogel were variable to cater to different wound environments.Furthermore,silver nanoparticles could be loaded into the void space of the hydrogel which endowed the composites with superior anti-infective properties.We have demonstrated that the bio-inspired platelet hydrogel could promote hemostasis of acute tissue damage,prevent bacterial proliferation,and promote angiogenesis,collagen deposition,and granulation tissue formation in wound healing.These features signify the potential values of the bio-inspired platelet hydrogel in clinical applications.
