目的:运用文献计量学与可视化分析,探索伤口生物膜的研究现状、热点及趋势。方法:检索Web of Science核心合集中伤口生物膜的相关文献,检索时间为2003年1月1日—2022年4月10日,采用VOSviewer、CiteSpace对检索结果进行分析。结果:共纳入...目的:运用文献计量学与可视化分析,探索伤口生物膜的研究现状、热点及趋势。方法:检索Web of Science核心合集中伤口生物膜的相关文献,检索时间为2003年1月1日—2022年4月10日,采用VOSviewer、CiteSpace对检索结果进行分析。结果:共纳入2050篇文献,综合指数排名居第1位的作者为丹麦的学者Bjarnsholt Thomas;载文量最多的期刊为Journal of Wound Care;发文量最多的国家为美国(679篇);发文量最多的机构是丹麦哥本哈根大学(40篇)。结论:伤口生物膜相关研究的主力在美国,焦点为伤口生物膜的种类、特性等基础研究及对抗生物膜的方法方面。未来需加强机构间的交流合作,多关注伤口生物膜的临床管理及其社会学评价等方面的研究。展开更多
Objective： To explore the feasibility of using regenerated silk fibroin membrane to construct artificial skin substitutes for wound healing, it is necessary to evaluate its cytocompatibility. Methods： The effects of...Objective： To explore the feasibility of using regenerated silk fibroin membrane to construct artificial skin substitutes for wound healing, it is necessary to evaluate its cytocompatibility. Methods： The effects of regenerated silk fibroin film on cytotoxicity, adhesion, cell cycle, and apoptosis of L929 cells, growth and vascular endothelial growth factor （VEGF） expression of ECV304 cells, and VEGF, angiopoietin-1 （Ang-1）, platelet-derived growth factor （PDGF） and fibroblast growth factor 2 （FGF2） expression of WI-38 cells were assessed by 3-（4,5）-dimethylthiahiazo （-z-yl）-3,5-di-phenytetrazoliumromide （MTT） assay, viable cell counting, flow cytometry （FCM）, and enzyme-linked immunosorbant assay （ELISA）. Results： We showed that the regenerated silk fibroin film was not cytotoxic to L929 cells and had no adverse influence on their adhesion, cell cycle or apoptosis; it had no adverse influence on the growth and VEGF secretion of ECV304 cells and no effect on the secretion of VEGF, Ang-1, PDGF and FGF2 by WI-38 cells. Conclusion： The regenerated silk fibroin film should be an excellent biomaterial with good cytocompatibility, providing a framework for reparation after trauma in clinical applications.展开更多
文摘目的:运用文献计量学与可视化分析,探索伤口生物膜的研究现状、热点及趋势。方法:检索Web of Science核心合集中伤口生物膜的相关文献,检索时间为2003年1月1日—2022年4月10日,采用VOSviewer、CiteSpace对检索结果进行分析。结果:共纳入2050篇文献,综合指数排名居第1位的作者为丹麦的学者Bjarnsholt Thomas;载文量最多的期刊为Journal of Wound Care;发文量最多的国家为美国(679篇);发文量最多的机构是丹麦哥本哈根大学(40篇)。结论:伤口生物膜相关研究的主力在美国,焦点为伤口生物膜的种类、特性等基础研究及对抗生物膜的方法方面。未来需加强机构间的交流合作,多关注伤口生物膜的临床管理及其社会学评价等方面的研究。
基金supported by the National Basic Research Program (973) of China (No.2005CB623906)the Medical Development Foundation of Soochow University (No.EE134702),China
文摘Objective： To explore the feasibility of using regenerated silk fibroin membrane to construct artificial skin substitutes for wound healing, it is necessary to evaluate its cytocompatibility. Methods： The effects of regenerated silk fibroin film on cytotoxicity, adhesion, cell cycle, and apoptosis of L929 cells, growth and vascular endothelial growth factor （VEGF） expression of ECV304 cells, and VEGF, angiopoietin-1 （Ang-1）, platelet-derived growth factor （PDGF） and fibroblast growth factor 2 （FGF2） expression of WI-38 cells were assessed by 3-（4,5）-dimethylthiahiazo （-z-yl）-3,5-di-phenytetrazoliumromide （MTT） assay, viable cell counting, flow cytometry （FCM）, and enzyme-linked immunosorbant assay （ELISA）. Results： We showed that the regenerated silk fibroin film was not cytotoxic to L929 cells and had no adverse influence on their adhesion, cell cycle or apoptosis; it had no adverse influence on the growth and VEGF secretion of ECV304 cells and no effect on the secretion of VEGF, Ang-1, PDGF and FGF2 by WI-38 cells. Conclusion： The regenerated silk fibroin film should be an excellent biomaterial with good cytocompatibility, providing a framework for reparation after trauma in clinical applications.
