目的:探讨孕期恐伤肾对其56日龄仔鼠股骨发育和血清中骨代谢指标的影响,为“恐伤肾,肾主骨”理论提供实验依据。方法:选取基线一致的孕0.5 d Wistar大鼠20只按照随机数字表法分为模型组和对照组,模型组运用旁观电击法建立妊娠期恐伤大...目的:探讨孕期恐伤肾对其56日龄仔鼠股骨发育和血清中骨代谢指标的影响,为“恐伤肾,肾主骨”理论提供实验依据。方法:选取基线一致的孕0.5 d Wistar大鼠20只按照随机数字表法分为模型组和对照组,模型组运用旁观电击法建立妊娠期恐伤大鼠模型,对照组正常喂养,分别在孕7 d、14 d和20 d采用旷场实验对孕鼠进行行为学测试,在孕20 d采用酶联免疫法(ELISA)检测其血清糖皮质激素(GC)水平并评价建模情况。仔鼠延续母鼠分组,利用旷场实验对56日龄仔鼠进行行为学测试,采集血清和双后肢股骨样本并检测钙磷水平。用ELISA检测血清中I型胶原羧基端交联肽(CTX-I)、骨源性碱性磷酸酶(BALP)和I型前胶原氨基端原肽(PINP)水平。结果:与对照组比较,模型组孕鼠在孕7 d、14 d和20 d的旷场实验水平得分、垂直得分均降低(P<0.05),孕20 d GC水平显著升高(P<0.05);与对照组比较,模型组56日龄仔鼠旷场实验水平得分、垂直得分均降低(P<0.05),体质量减轻,肾脏质量和脏器系数均降低(P<0.05);双后肢股骨干重降低(P<0.05),股骨钙磷含量也均降低(P<0.05),血清中钙含量降低(P<0.05),CTX-I水平显著升高(P<0.05),BALP和PINP的水平均降低(P<0.05)。结论:恐伤孕鼠可影响其子代的股骨发育和代谢,恐惧刺激可以引起骨发育损伤,主要表现为钙磷代谢紊乱和骨吸收增强。展开更多
Objective] This study aimed to investigate the effects of glycidamide （GA） on growth and progesterone biosynthesis of rat R2C Leydig cel s cultured in vitro. [Method] The R2C Leydig cel s were treated with GA with c...Objective] This study aimed to investigate the effects of glycidamide （GA） on growth and progesterone biosynthesis of rat R2C Leydig cel s cultured in vitro. [Method] The R2C Leydig cel s were treated with GA with concentrations of 0.25, 0.5, 0.75, 1, 2, 4 and 6 mmol/L respectively for 48 h. The IC25, IC50 and IC75 values of GA were al detected by MTT assay. The Leydig cel s were treated with GA at concentrations of IC25, IC50 and IC75 respectively for 48 h, and then the morphology of Leydig cel s was observed. After the Leydig cel s were treated with GA for 4 h, the cel ular DNA damage was measured by the comet assay technique; and after the Leydig cel s were with treated with GA for 24 h, the progesterone biosynthesis amount was detected by radioimmunoassay （RIA）. [Result] GA could inhibit the via-bility of R2C Leydig cel s, and its IC25, IC50 and IC75 were 0.635, 0.872 and 1.198 mmol/L, respectively. The GA at concentrations of IC25, IC50 and IC75 affected the growth and morphology of rat R2C Leydig cel s in varying degrees. The 4-h treat-ment of GA could significantly damage the DNA of R2C Leydig cel s, and the 24-h treatment of GA at concentrations of IC25, IC50 and IC75 al reduced the progesterone biosynthesis amount. [Conclusion] GA could inhibit the growth and progesterone biosynthesis of rat R2C Leydig cel s.展开更多
Objective: To explore the characteristics of closed abdominal injury in pregnancy women and its treatment.Methods: The clinical data of 37 pregnancy patients with closed abdominal injury treated in our hospital from J...Objective: To explore the characteristics of closed abdominal injury in pregnancy women and its treatment.Methods: The clinical data of 37 pregnancy patients with closed abdominal injury treated in our hospital from June 1993 to June 2003 were collected and analyzed.Results: All the 37 patients were treated with operation. Among them 2 early pregnancy patients with intestinal rupture and 1 patient with retroperitoneal hematoma were treated under laparoscope; in other 34 pregnancy patients laparotomy was performed. Of the 34 patients 8 used cesarean section because premature separation of placenta and enlarged womb interrupted the management of intra-abdominal organ injury. In the 37 patients 33 ( 89.1%) were cured, 4 ( 10.8%) die, postoperative complication rate was 16.2% (6/37). Two patients ( 5.4%) suffered from abdominal cavity infection, 3 ( 8.1%) from pulmonary infection, and 1 ( 2.7%) had multi-organ failure.Conclusions: For pregnancy patients with closed abdominal injury, besides obsteric diseases intra-abdominal injury should be given much attention. Accurate diagnosis and timely treatment can gain the time to save the life of both mother and fetus.展开更多
基金Supported by Training Program for Outstanding Young Teachers in Higher Education Institutions,Guangdong Province(Yq2013024)Program for New Century Excellent Talents in University of Ministry of Education of ChinaNational Natural Science Foundation of China(31201402,31201340)~~
文摘Objective] This study aimed to investigate the effects of glycidamide （GA） on growth and progesterone biosynthesis of rat R2C Leydig cel s cultured in vitro. [Method] The R2C Leydig cel s were treated with GA with concentrations of 0.25, 0.5, 0.75, 1, 2, 4 and 6 mmol/L respectively for 48 h. The IC25, IC50 and IC75 values of GA were al detected by MTT assay. The Leydig cel s were treated with GA at concentrations of IC25, IC50 and IC75 respectively for 48 h, and then the morphology of Leydig cel s was observed. After the Leydig cel s were treated with GA for 4 h, the cel ular DNA damage was measured by the comet assay technique; and after the Leydig cel s were with treated with GA for 24 h, the progesterone biosynthesis amount was detected by radioimmunoassay （RIA）. [Result] GA could inhibit the via-bility of R2C Leydig cel s, and its IC25, IC50 and IC75 were 0.635, 0.872 and 1.198 mmol/L, respectively. The GA at concentrations of IC25, IC50 and IC75 affected the growth and morphology of rat R2C Leydig cel s in varying degrees. The 4-h treat-ment of GA could significantly damage the DNA of R2C Leydig cel s, and the 24-h treatment of GA at concentrations of IC25, IC50 and IC75 al reduced the progesterone biosynthesis amount. [Conclusion] GA could inhibit the growth and progesterone biosynthesis of rat R2C Leydig cel s.
文摘Objective: To explore the characteristics of closed abdominal injury in pregnancy women and its treatment.Methods: The clinical data of 37 pregnancy patients with closed abdominal injury treated in our hospital from June 1993 to June 2003 were collected and analyzed.Results: All the 37 patients were treated with operation. Among them 2 early pregnancy patients with intestinal rupture and 1 patient with retroperitoneal hematoma were treated under laparoscope; in other 34 pregnancy patients laparotomy was performed. Of the 34 patients 8 used cesarean section because premature separation of placenta and enlarged womb interrupted the management of intra-abdominal organ injury. In the 37 patients 33 ( 89.1%) were cured, 4 ( 10.8%) die, postoperative complication rate was 16.2% (6/37). Two patients ( 5.4%) suffered from abdominal cavity infection, 3 ( 8.1%) from pulmonary infection, and 1 ( 2.7%) had multi-organ failure.Conclusions: For pregnancy patients with closed abdominal injury, besides obsteric diseases intra-abdominal injury should be given much attention. Accurate diagnosis and timely treatment can gain the time to save the life of both mother and fetus.
