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CRTSⅡ型板式无砟轨道支承层斜裂伤损修复方法 被引量:8
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作者 黄传岳 《铁道建筑》 北大核心 2018年第11期139-141,共3页
针对CRTSⅡ型板式无砟轨道支承层斜裂伤损,确定了升温膨胀是其伤损的主要原因。在保证列车不中断运营的前提下,提出了钢轨切割、轨道板移除、CA砂浆清理、钢垫梁临时支撑、伤损支承层修复、轨道板复位、砂浆层灌注的结构快速修复作业方... 针对CRTSⅡ型板式无砟轨道支承层斜裂伤损,确定了升温膨胀是其伤损的主要原因。在保证列车不中断运营的前提下,提出了钢轨切割、轨道板移除、CA砂浆清理、钢垫梁临时支撑、伤损支承层修复、轨道板复位、砂浆层灌注的结构快速修复作业方案,并配套研发了具有快硬早强特性的CA砂浆材料和聚合物混凝土。应用实践表明,该方法占用天窗数量少,结构恢复快,修复后的轨道几何尺寸能够满足运营需求。可进一步应用于无砟轨道纵连体系结构维修中。 展开更多
关键词 无砟轨道 支承层 裂缝 伤损修复 现场试验 钢垫梁 快硬聚合物
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Fanconi Anemia and Ubiquitination 被引量:4
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作者 张莹莹 周晓巍 黄培堂 《Journal of Genetics and Genomics》 SCIE CAS CSCD 北大核心 2007年第7期573-580,共8页
Fanconi anemia (FA) is a rare recessive hereditary disease characterized clinically by congenital defects, progressive bone-marrow failure, and cancer predisposition. Cells from FA patients exhibit hypersensitivity ... Fanconi anemia (FA) is a rare recessive hereditary disease characterized clinically by congenital defects, progressive bone-marrow failure, and cancer predisposition. Cells from FA patients exhibit hypersensitivity to DNA cross-linking agents, such as mitomycin C (MMC). To date, at least 12 FA genes have been found deleted or mutated in FA cells, and 10 FA gene products form a core complex involved in FA/BRCA2 DNA repair pathway-FA pathway. The ubiquitin E3 ligase FANCL, an important factor of FA core complex, co-functions with a new ubiquitin conjugating enzyme UBE2T to catalyze the monoubiquitination of FANCD2. FANCD2-Ub binds BRCA2 to form a new complex located in chromatin foci and then take part in DNA repair process. The deubiquitylating enzyme USP1 removes the mono-ubiquitin from FANCD2-Ub following completion of the repair process, then restores the blocked cell cycle to normal order by shutting off the FA pathway. In a word, the FANCD2 activity adjusted exquisitely by ubiquitination and/or deubiquitination in vivo may co-regulate the FA pathway involving in variant DNA repair pathway. 展开更多
关键词 Fanconi anemia FA pathway UBIQUITINATION DNA repair
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Roles of NF-κB in central nervous system damage and repair 被引量:3
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作者 杨丽 陶陆阳 陈溪萍 《Neuroscience Bulletin》 SCIE CAS CSCD 2007年第5期307-313,共7页
NF-κB family is a kind of nuclear factors in B lymphocyte that can bind to the immunoglobulin κ-chain enhancer and enhance transcriptional activity. NF-κB/Rel proteins, as a dimeric transcription factor, control th... NF-κB family is a kind of nuclear factors in B lymphocyte that can bind to the immunoglobulin κ-chain enhancer and enhance transcriptional activity. NF-κB/Rel proteins, as a dimeric transcription factor, control the expression of genes that regulate a broad range of biological processes through canonical and non-canonical pathways. In the central nervous system, NF-κB controls inflammatory reactions and the apoptotic cell death following nerve injury. It also contributes to the infarction and cell death in stroke models and patients. However, NF-κB is essential for neurosurvival as well. NF-κB activation is a part of recovery process that may protect neurons against oxidative-stresses or brain ischemia-induced apoptosis and neurodegeneration. Inhibition of NF-κB may reduce its neuroprotection activity. Hence the dual opposite effects of NF-κB on cells. The ultimate survival or death of neurons depends on which, where and when the NF-κB factors are activated. 展开更多
关键词 NF-ΚB central nervous system INJURY REPAIR
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DNA Damage and Repair of Two Ecotypes of Phragmites communis Subjected to Water Stress 被引量:3
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作者 王俊刚 张承烈 《Acta Botanica Sinica》 CSCD 2001年第5期490-494,共5页
In order to thoroughly understand the mechanism Of drought resistance in plants at DNA level, the DNA damage of two ecotypes of reeds (Phragmites communis T.) stressed by PEG 6000 was analyzed by means of fluorescence... In order to thoroughly understand the mechanism Of drought resistance in plants at DNA level, the DNA damage of two ecotypes of reeds (Phragmites communis T.) stressed by PEG 6000 was analyzed by means of fluorescence analysis of DNA unwinding (FADU). The results showed that the residual double strand DNA percentages (dsDNA%) in dune reed (DR) were significantly higher than those in swamp reed (SR) treated with either 20% or 30% PEG 6000. This meant that the DNA of DR was less damaged in comparison with SR. Similarly, DR resisted DNA damage more strongly than SR as reactive oxygen species (ROS) increased by adding ROS producers diethyldithio carbamate (DDC), H2O2 and Fe2+ of different concentrations. Meanwhile, treating PEG stressed SR with ROS scavengers such as dimethyl sulphoxide (DMSO) and ascorbic acid (Vc) resulted in the reduction of DNA damage, suggesting that ROS could cause DNA damage. In addition, the DNA repair for water-stressed reeds indicated that DR repaired DNA damage much faster and more completely. This might be the first indication that drought stress led to DNA damage in plants and that drought resistance of plants was closely related to DNA damage and repair. 展开更多
关键词 dune reed swamp reed water stress reactive oxygen species DNA damage of plants in vivo DNA repair
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用于钢轨焊接接头补强的环氧类胶粘剂研制与应用
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作者 赵升龙 刘清方 梁滨 《现代城市轨道交通》 2021年第4期80-84,共5页
介绍用于轨道交通钢轨焊接接头补强的聚氨酯改性环氧胶粘剂的研制,并介绍其性能及在工厂的应用情况。该胶粘剂可在室温固化并具有较好的胶接性能、耐介质性能和耐湿热性能,使用温度为-55~70℃,可用于轨道交通钢轨焊接接头的补强和伤损... 介绍用于轨道交通钢轨焊接接头补强的聚氨酯改性环氧胶粘剂的研制,并介绍其性能及在工厂的应用情况。该胶粘剂可在室温固化并具有较好的胶接性能、耐介质性能和耐湿热性能,使用温度为-55~70℃,可用于轨道交通钢轨焊接接头的补强和伤损修复。 展开更多
关键词 轨道交通 钢轨 焊接接头补强 伤损修复 环氧胶粘剂
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Relationship between acute kidney injury before thoracic endovascular aneurysm repair and in-hospital outcomes in patients with type B acute aortic dissection 被引量:8
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作者 Hong-Mei REN Xiao WANG +5 位作者 Chun-Yan HU Bin QUE Hui AI Chun-Mei WANG Li-Zhong SUN Shao-Ping NIE 《Journal of Geriatric Cardiology》 SCIE CAS CSCD 2015年第3期232-238,共7页
Objective Acute kidney injury (AKI) frequently occurs after catheter-based interventional procedures and increases mortality. However, the implications of AKI before thoracic endovascular aneurysm repair (TEVAR) o... Objective Acute kidney injury (AKI) frequently occurs after catheter-based interventional procedures and increases mortality. However, the implications of AKI before thoracic endovascular aneurysm repair (TEVAR) of type B acute aortic dissection (AAD) remain unclear. This study evaluated the incidence, predictors, and in-hospital outcomes of AKI before TEVAR in patients with type B AAD. Methods Between 2009 and 2013, 76 patients were retrospectively evaluated who received TEVAR for type B AAD within 36 h from symptom onset. The patients were classified into no-AKI vs. AKI groups, and the severity of AKI was further staged according to kidney disease: im- proving global outcomes criteria before TEVAR. Results The incidence of preoperative AKI was 36.8%. In-hospital complications was significantly higher in patients with preoperative AKI compared with no-AKI (50.0% vs. 4.2%, respectively; P 〈 0.001), including acute renal failure (21.4% vs. O, respectively; P 〈 0.001), and they increased with severity of AKI (P 〈 0.001). The maximum levels of body tem- perature and white blood cell count were significantly related to maximum serum creatinine level before TEVAR. Multivariate analysis showed that systolic blood pressure on admission (OR: 1.023; 95% CI: 1.003-1.044; P : 0.0238) and bilateral renal artery involvement (OR: 19.076; 95% CI: 1.914 190.164; P = 0.0120) were strong predictors of preoperative AKI. Conclusions Preoperative AKI frequently occurred in patients with type B AAD, and correlated with higher in-hospital complications and enhanced inflammatory reaction. Systolic blood pressure on admission and bilateral renal artery involvement were major risk factors for AKI before TEVAR. 展开更多
关键词 Acute aortic dissection Kidney injury Renal failure Thoracic endovascular aneurysm repair
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Cell and gene therapy for arrhythmias: Repair of cardiac conduction damage 被引量:3
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作者 Yong-Fu Xiao 《Journal of Geriatric Cardiology》 SCIE CAS CSCD 2011年第3期147-158,共12页
Action potentials generated in the sinoatrial node (SAN) dominate the rhythm and rate of a healthy human heart. Subsequently, these action potentials propagate to the whole heart via its conduction system. Abnormali... Action potentials generated in the sinoatrial node (SAN) dominate the rhythm and rate of a healthy human heart. Subsequently, these action potentials propagate to the whole heart via its conduction system. Abnormalities of impulse generation and/or propagation in a heart can cause arrhythmias. For example, SAN dysfunction or conduction block of the atrioventricular node can lead to serious bradycardia which is currently treated with an implanted electronic pacemaker. On the other hand, conduction damage may cause reentrant tachyarrhythmias which are primarily treated pharmacologically or by medical device-based therapies, including defibrillation and tissue ablation. However, drug therapies sometimes may not be effective or are associated with serious side effects. Device-based therapies for cardiac arrhythmias, even with well developed technology, still face inadequacies, limitations, hardware complications, and other challenges. Therefore, scientists are actively seeking other alternatives for antiarrhythmic therapy. In particular, cells and genes used for repairing cardiac conduction damage/defect have been investigated in various studies both in vitro and in vivo. Despite the complexities of the excitation and conduction systems of the heart, cell and gene-based strategies provide novel alternatives for treatment or cure of cardiac arrhythmias. This review summarizes some highlights of recent research progress in this field. 展开更多
关键词 cell therapy gene therapy conduction repair ARRHYTHMIA
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Protective effects of Rad23 protein on ultraviolet damage to HeLa cells
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作者 李秀山 张继红 +1 位作者 赵小英 刘选明 《Journal of Central South University》 SCIE EI CAS 2013年第11期2974-2980,共7页
Protein Rad23, a nucleotide excision repair factor, mainly involves in repairing the DNA damage from environment, such as UV light. The function of Rad23 protein involved in DNA damage repair from many environmental f... Protein Rad23, a nucleotide excision repair factor, mainly involves in repairing the DNA damage from environment, such as UV light. The function of Rad23 protein involved in DNA damage repair from many environmental factors has been studied extensively, but it is not clear from ultraviolet irradiation. To further investigate the photo-protective function of Rad23 protein on HeLa cells damaged from UV light irradiation, firstly, HeLa cells were irradiated by UV light and incubated with the fusion protein of pCold-Rad23, then the cell viability and apoptosis rate were detected by MTT and Hoechst33342/Pl fluorescent staining, respectively. The results show that the recombinant Rad23 protein can protect the HeLa cells from UV irradiation, and inhibit the apoptosis of HeLa cell by UV irradiation. 展开更多
关键词 Rad23 protein nucleotide excision repair HeLa cell INJURY
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Transferring superior gluteal nerve to pudendal nerve in reconstructing functional impairment in simple conus medullaris or pudendal nerve injury
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作者 党瑞山 刘芳 +5 位作者 张少成 李玉泉 黄会龙 陈尔瑜 许家军 张传森 《Journal of Medical Colleges of PLA(China)》 CAS 2003年第6期337-340,共4页
Objective: 1Department of Anatomy, College of Basic Medical Sciences, Second Military Medical University, Shanghai 200433, China 2Department of Orthopedic Surgery, Changhai Hospital, Second Military Medical University... Objective: 1Department of Anatomy, College of Basic Medical Sciences, Second Military Medical University, Shanghai 200433, China 2Department of Orthopedic Surgery, Changhai Hospital, Second Military Medical University, Shanghai 200433, China Abstract Objective: To study the anatomical basis of transferring the superior gluteal nerve to the pudendal nerve in reconstructing the functional impairment in simple conus medullaris or pudendal nerve injury. Methods: Superior gluteal nerve and pudendal nerve were observed and measured by the gross and microsurgical anatomical methods in 62 sides of 31 adult cadavers. Results: Superior gluteal nerve came out of the superior foreman of piriformis as 1 to 4 branches(29.03%,56.45%,12.90% and 1.61% respectively) and the pelvic-leaving points were mainly in the middle 1/3(85.48%) of the line from the posterior superior iliac spine to the ischial tuberosity. The length of the inferior branch of the superior gluteal nerve was more than 5 cm, and the distance between the pelvic-leaving points of the superior gluteal nerve and pudandal nerve was about 4 cm only. The pudendal nerve left the pelvis mainly in the middle 1/3(48.39%) of the line from the posterior superior iliac spine to the ischial tuberosity,or at the junction of its inferior-middle 1/3(46.77%). In clinic, we have successfully made the operation transferring the superior gluteal nerve to the pudendal nerve in 3 patients suffered from the injury of conus medullaris. Conclusion: Distance between the pelvic-leaving points of the superior gluteal nerve and the pudendal nerve is close, so the inferior branch of the gluteal nerve can be anastomosed with the pudendal nerve directly. Transferring the superior gluteal nerve with higher spinal segemental origin to the pudendal nerve of a lower spinal segemental origin is practical and easy. 展开更多
关键词 superior gluteal nerve pudendal nerve nerve transfer functional reconstruction
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Peripheral nerve repair by conduits made of human hair keratin: An experimental study
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作者 胡庆柳 朴英杰 《Journal of Medical Colleges of PLA(China)》 CAS 2002年第1期10-13,共4页
Objective: To explore the method to repair injured peripheral nerve using conduits made of human hair keratin (HHK). Methods: The tibial nerves of rabbits were transected leaving a gap 10 mm in length between the 2 se... Objective: To explore the method to repair injured peripheral nerve using conduits made of human hair keratin (HHK). Methods: The tibial nerves of rabbits were transected leaving a gap 10 mm in length between the 2 severed ends, which were either routinely sutured or bridged using HHK nerve conduits. Electro-physiological , anatomical and histological examinations were performed at different time postoperatively. Results: Electrophysiological study showed more obvious improvement in the neural function recovery in rabbits with HHK conduits bridging as compared with that in rabbits with routine suture. In the former group, HHK conduits were gradually degraded and absorbed with large amount of myelinated nerve fibers and Schwann cells regenerated around HHK conduits. In the latter group, however, the nerve tissues around the suture were degenerated and replaced by connective tissues. Conclusion: HHK may induce the regeneration of the nerve fibers and provides an ideal approach to repair nerve damages. 展开更多
关键词 human hair keratin tibial nerve REPAIR
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Effects of UV-B radiation on tetraspores of Chondrus ocellatus Holm(Rhodophyta),and effects of red and blue light on repair of UV-B-induced damage 被引量:5
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作者 鞠青 肖慧 +1 位作者 王悠 唐学玺 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 2015年第3期650-663,共14页
We evaluated the effects of red and blue light on the repair of UV-B radiation-induced damage in tetraspores of Chondrus ocellatus Holm. Tetraspores of C. ocellatus were treated with different UV-B radiation levels(0,... We evaluated the effects of red and blue light on the repair of UV-B radiation-induced damage in tetraspores of Chondrus ocellatus Holm. Tetraspores of C. ocellatus were treated with different UV-B radiation levels(0,36,72,108,144 and 180 J/m2),and thereafter subjected to PAR,darkness,or red or blue light during a 2-h repair stage,each day for 48 days. The diameters and cellular contents of cyclobutane pyrimidine dimmers(CPDs),chlorophyll a(Chl a),phycoerythrin,and UV-B-absorbing mycosporinelike amino acids(MAAs) contents of the tetraspores were determined. Our results show that low doses of UV-B radiation(36 and 72 J/m 2) promoted the growth of C. ocellatus; however,increased UV-B radiation gradually reduced the C. ocellatus growth(greater than 72 J/m2). The MAAs(palythine and asterina-330) in C. ocellatus were detected and analyzed by LC/MS. Our results suggest that moderate red light could induce the growth of this alga in aquaculture. In addition,photorepair was inhibited by red light,so there may be some other DNA repair mechanism activated by red light. Blue light promoted the activity of DNA photolyase,greatly improving remediation efficiency. Red and blue lights were found to reduce the capacity of C. ocellatus to form MAAs. Therefore,PAR,red light,and blue light play different roles during the repair processes for damage induced by UV-B radiation. 展开更多
关键词 Chondrus ocellatus Holm tetraspores UV-B radiation red light blue light REPAIR
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The DNA injury-repair of esophageal cancer EC109 cells to diallyl trisulfide (DATS) combining radiation
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作者 Yang Zhang Hongbing Ma 《The Chinese-German Journal of Clinical Oncology》 CAS 2012年第11期644-646,共3页
Objective: The aim of our study was to investigate the effect of diallyl trisulfide (DATS) combining radiation on DNA injury-repair of Esophageal cancer EC109 cells. Methods: Using 10 and 20 μg/mL DATS on EC109 cells... Objective: The aim of our study was to investigate the effect of diallyl trisulfide (DATS) combining radiation on DNA injury-repair of Esophageal cancer EC109 cells. Methods: Using 10 and 20 μg/mL DATS on EC109 cells, and taking X-ray radiation 24 h later. Investigate the radiosensitization effect of DATS on EC109 cells by clone formation, and the mechanism of DNA injury-repair by Comet Assay. Results: The clone formation resulted that DATS had radiosensitization effect on EC109 cells. Radiosensitization enhancement ratios of 10 and 20 μg/mL DATS in combination with radiation were 1.55, 1.64 (Do) and 1.43, 1.75 (Dq) respectively. In the comet assay, the TM (tail moments) of 20 μg/mL DATS combining radiation group lines at 0 h, 2 h, 6 h and 24 h were 7.16 ± 2.61, 3.65 ± 2.06, 2.09 ± 0.83, 1.45 ± 1.37 respectively. They were slightly increased than radiation group (0.95 ± 0.65, 0.11 ± 0.07, 0.1 ± 0.05, 0.11 ± 0.08) and DATS group (1.81 ± 1.23, 1.58 ± 1.40, 0.45 ± 0.25, 0.60 ± 0.40) (P < 0.01). The result showed that DATS combining radiation had the effect of increasing DNA damage and inhibiting DNA repair on EC109 cells. Conclusion: DATS has radiosensitization effect on Esophageal cancer EC109 cells. And the effect is probably related with DNA injury-repair. 展开更多
关键词 diallyl trisulfide (DATS) comet assay DNA injury DNA repair
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Investigation of the active components and mechanism of Sanao Decoction in treating chronic cough by network pharmacology and molecular docking 被引量:2
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作者 SHENG Mengke LIU Xing +4 位作者 LIAO Yuyao LI Zhixun LYU Lijing YANG Jiaqi SHI Xinyuan 《Digital Chinese Medicine》 2021年第3期191-201,共11页
Objective To investigate the active components and mechanism of Sanao Decoction(三拗汤,SAD)in treating chronic cough based on network pharmacology and molecular docking.Methods Active components and their targets were... Objective To investigate the active components and mechanism of Sanao Decoction(三拗汤,SAD)in treating chronic cough based on network pharmacology and molecular docking.Methods Active components and their targets were obtained from the Traditional Chinese Medicine Systems and Pharmacology Database and Analysis Platform(TCMSP),Bioinformatics Analysis Tool for Molecular mech ANism of Traditional Chinese Medicine(BATMAN-TCM)database,and the literature.The component-target regulatory network and protein-protein interaction(PPI)network were constructed by Cytoscape 3.7.2,and a bioinformatics analysis was performed to identify the significant pathways and their relevant targets.Molecular docking of the core active components and relevant targets was performed.Results A total of 98 active components of SAD and the corresponding 113 drug targets were identified.The component-target regulatory network and PPI network were successfully established.Results of the bioinformatics analysis indicated that 2281 Gene Ontology(GO)terms were enriched in chronic cough,including 2062 terms were in biological processes,77 in cellular components,and 142 in molecular functions,and top 20 significant pathways in Kyoto Encyclopedia of Genes and Genomes(KEGG)analysis.Molecular docking study demonstrated that quercetin,luteolin,kaempferol,and naringenin were in good agreement with the corresponding targets.Conclusion The active compounds of SAD,such as quercetin,luteolin,kaempferol,and naringenin,may act on AKT1,MAPK1,RELA,EGFR,and Bcl-2 and regulate the PI3 K-Akt signaling pathway,AGE-RAGE signaling pathway,and fluid shear stress and atherosclerosis pathway to exert the effects of anti-inflammatory,anti-airway remodeling,anti-oxidant stress effects,and repair airway damage,thus treating chronic cough. 展开更多
关键词 Sanao Decoction(三拗汤 SAD) Chronic cough Network pharmacology Molecular docking Anti-airway remodeling Anti-oxidant stress Repair airway damage
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Modeling of DSBs Generation and Repair Process under Ion Radiation
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作者 齐金鹏 邵世煌 +1 位作者 谢锦丽 白慧 《Journal of Donghua University(English Edition)》 EI CAS 2007年第4期484-487,493,共5页
Under acute perturbations from outside, cell can trigger the self-defense mechanisms in fighting against these genome stresses. To simulate the investigation of the complicated mechanisms of cellular responding DNA da... Under acute perturbations from outside, cell can trigger the self-defense mechanisms in fighting against these genome stresses. To simulate the investigation of the complicated mechanisms of cellular responding DNA damage at single cell level, a model of the double strand breaks (DSBs) generation and repair process is proposed under continuous effect of acute IR. Under different IR dose domains, this model can be used to simulate the complicated interactions among vital components within the cell, and the plausible outcomes of cellular response in fighting against DNA damage. 展开更多
关键词 P53 DNA damage IR Repair Protein Modeling
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Implications of oxidative damage to proteins and DNA in aging and its intervention by caloric restriction and exercise
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作者 Sataro Goto Zsolt Radak 《Journal of Sport and Health Science》 SCIE 2013年第2期75-80,共6页
In this short review we describe implications of age-related changes of protein and DNA oxidation as a public mechanism of biological aging. Oxidatively modified protein and DNA have been demonstrated to increase with... In this short review we describe implications of age-related changes of protein and DNA oxidation as a public mechanism of biological aging. Oxidatively modified protein and DNA have been demonstrated to increase with advancing age in rodents. Half-life of proteins is extended and DNA repair activity declines in old animals. Dietary restriction initiated late in life can shorten the half-life of proteins to levels of young animals, thus contributing to reduce level of altered proteins in old animals by the regimen. Regular exercise reduced oxidatively modified proteins in the brain with improved cognitive functions. It attenuated oxidative stress in the liver, i.e., ameliorating activation of nuclear factor κB, increasing reduced glutathione, and decreasing oxidized guanine base in nuclear and mitochondrial DNA. These findings suggest that regular exercise has systemic effects in reducing oxidative stress. Thus, life-styles such as diet and exercise may extend health span, by up-regulating overall anti-oxidant capacities that include proteins involved in protein turnover and DNA repair, resulting in reduction of damaged proteins and DNA that potentially promote physiological and pathological aging. 展开更多
关键词 AGING Dietary restriction DNA EXERCISE Oxidative stress Protein
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Repair of a mal-repaired biliary injury:A case report
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作者 Awad Aldumour Paolo Aseni +4 位作者 Mohmmad Alkofahi Luca Lamperti Elias Aldumour Paolo Girotti Luciano Gregorio De Carlis 《World Journal of Gastroenterology》 SCIE CAS CSCD 2009年第18期2283-2286,共4页
Iatrogenic bile-duct injury post-laparoscopic cholecystectomy remains a major serious complication with unpredictable long-term results. We present a patient who underwent laparoscopic cholecystectomy for gallstones, ... Iatrogenic bile-duct injury post-laparoscopic cholecystectomy remains a major serious complication with unpredictable long-term results. We present a patient who underwent laparoscopic cholecystectomy for gallstones, in which the biliary injury was recognized intraoperatively. The surgical procedure was converted to an open one. The first surgeon repaired the injury over a T-tube without recognizing the anatomy and type of the biliary lesion, which led to an unusual biliary mal-repair. Immediately postoperatively, the abdominal drain brought a large amount of bile. A T-tube cholangiogram was performed. Despite the contrast medium leaking through the abdominal drain, the mal-repair was recognized intraoperatively. The surgical procedure was converted to an open one. The first surgeon repaired the injury over a T-tube without recognizing the anatomy and type of the biliary lesion, which led to an unusual biliary mal-repair. Immediately postoperatively, the abdominal drain brought a large amount of bile. A T-tube cholangiogram was performed. Despite the contrast medium leaking through the abdominal drain, the mal-repair was unrecognized. The patient was referred to our hospital for biliary leak. Ultrasound and cholangiography was repeated, which showed an unanatomical repair (right to left hepatic duct anastomosis over the T-tube),with evidence of contrast medium coming out through the abdominal drain. Eventually the patient was subjected to a definitive surgical treatment. The biliary continuity was re-established by a Roux-en-Y hepaticojejunostomy, over transanastomotic external biliary stents. The patient is now doing well 4 years after the second surgical procedure. In reviewing the literature, we found a similar type of injury but we did not find a similar surgical real-repair. We propose an algorithm for the treatment of early and late biliary injuries. 展开更多
关键词 Biliary tract injury Surgical complication Biliary surgery Laparoscopic cholecystectomy
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Optimization-based conformal path planning for in situ bioprinting during complex skin defect repair
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作者 Wenxiang Zhao Chuxiong Hu +3 位作者 Yunan Wang Shize Lin Ze Wang Tao Xu 《Bio-Design and Manufacturing》 2025年第1期1-19,I0001,共20页
The global demand for effective skin injury treatments has prompted the exploration of tissue engineering solutions.While three-dimensional(3D)bioprinting has shown promise,challenges persist with respect to achieving... The global demand for effective skin injury treatments has prompted the exploration of tissue engineering solutions.While three-dimensional(3D)bioprinting has shown promise,challenges persist with respect to achieving timely and compatible solutions to treat diverse skin injuries.In situ bioprinting has emerged as a key new technology,since it reduces risks during the implantation of printed scaffolds and demonstrates superior therapeutic effects.However,maintaining printing fidelity during in situ bioprinting remains a critical challenge,particularly with respect to model layering and path planning.This study proposes a novel optimization-based conformal path planning strategy for in situ bioprinting-based repair of complex skin injuries.This strategy employs constrained optimization to identify optimal waypoints on a point cloud-approximated curved surface,thereby ensuring a high degree of similarity between predesigned planar and surface-mapped 3D paths.Furthermore,this method is applicable for skin wound treatments,since it generates 3D-equidistant zigzag curves along surface tangents and enables multi-layer conformal path planning to facilitate the treatment of volumetric injuries.Furthermore,the proposed algorithm was found to be a feasible and effective treatment in a murine back injury model as well as in other complex models,thereby showcasing its potential to guide in situ bioprinting,enhance bioprinting fidelity,and facilitate improvement of clinical outcomes. 展开更多
关键词 In situ bioprinting Path planning Robot control Skin injury repair
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Relationship between transmembrane signal transduction pathway and DNA repair and the mechanism after global cerebral ischemia-reperfusion in rats 被引量:2
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作者 薛荣亮 何家璇 +3 位作者 王宁 姚凤珍 吕建瑞 吴刚 《Neuroscience Bulletin》 SCIE CAS CSCD 2009年第3期115-121,共7页
Objective To !nvestigate the protein levels of phospho-ERK and phospho-APE/Ref-1 in hippocampal neurons after global cerebral ischemia reperfusion in rats, and observe the relationship between transmembrane signal tra... Objective To !nvestigate the protein levels of phospho-ERK and phospho-APE/Ref-1 in hippocampal neurons after global cerebral ischemia reperfusion in rats, and observe the relationship between transmembrane signal transduction and repair of DNA damage. The role of ERK signal transduction pathway following global cerebral ischemia reperfusion in rats is further discussed. Methods Ninety healthy male SD rats were divided into 3 groups randomly: Sham group (S group), Ischemia reperfusion group (IR group) and Pd98059 pretreatment/ischemia reperfusion group (PD group). Global cerebral ischemia reperfusion model was established by four-vessel occlusion (4-VO) method, and reperfusion was performed 5 minutes following ischemia. Protein levels of phospho-ERK and phospho-APE/Ref-1 were detected using immunohistochemical method at 2 h, 6 h, 12 h, 24 h, 48 h and 72 h after reperfusion, and neuron apoptosis was observed by HE and TUNEL staining. Results In CA1 region of IR group, TUNEL positive cells began to appear at 6 h after IR, and reached the apex during 24 h to 48 h. However, TUNEL positive was most strongly exhibited in PD group. In IR group, phospho-ERK was obviously detected in CA3 region at 2 h after IR, and its level was phospho-ERK expression in PD group was weaker than gradually decreased from 6 h until totally absent at 48 h. Besides, that in IR group. For phospho-APE/Ref-1, its expression began to appear in CA1 region in IR group at 2 h after IR, with no obvious changes during 2 h to 12 h. Phospho-APE/Ref-1 expression began to decrease at 24 h and this decrease continued thereafter. Expression level of phospho-APE/Ref-1 in PD group was lower than that in IR group. Results showed the concurrence of decreased phospho-ERK expression level and increased neuron apoptosis after cerebral ischemia reperfusion, the former of which was consistent with the decrease of phospho-APE/ Ref- 1 expression. Also, the greater the inhibition of ERK phosphorylation was, the greater decrease of APE/Ref- 1 expression occurred. Conclusion Activation of ERK signal transduction pathway increased the expression of phospho-APE/Ref-1, and thus faciliated the repair of DNA damage. So, activation of ERK signal transduction pathway may protect neurons from apoptosis after cerebral ischemia reperfusion. 展开更多
关键词 cerebral ischemia/reperfusion injury ERK APE/REF-1 DNA repair APOPTOSIS
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Repair of acutely injured spinal cord through constructing tissue-engineered neural complex in adult rats 被引量:6
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作者 蒲渝 郭庆山 +3 位作者 王爱民 吴思宇 刑书星 张忠荣 《Chinese Journal of Traumatology》 CAS 2007年第3期171-176,共6页
Objective: To construct tissue-engineered neural complex in vitro and study its effect in repairing acutely injured spinal cord in adult rats. Methods: Neural stem cells were harvested from the spinal cord of embry... Objective: To construct tissue-engineered neural complex in vitro and study its effect in repairing acutely injured spinal cord in adult rats. Methods: Neural stem cells were harvested from the spinal cord of embryo rats and propagated in vitro. Then the neural stem cells were seeded into polygiycolic acid scaffolds and co-cultured with extract of embryonic spinal cord in vitro. ce histochemistry and scanning electron microscope were used to observe the microstructure of this complex. Animal model of spine semi-transection was made and tissue-engineered neural complex was implanted by surgical intervention. Six weeks after transplantation, functional evaluation and histochemistry were applied to evaluate the functional recovery and anatomic reconstruction. Results: The tissue-engineered neural complex had a distinct structure, which contained neonatal neurons, oligodendrocytes and astrocytes. After tissue-engineered neural complex was implanted into the injured spinal cord, the cell components such as neurons, astrocytes and oligodendrocytes, could survive and keep on developing. The adult rats suffering from spinal cord injury got an obvious neurological recovery in motor skills. Conclusions: The tissue-engineered neural complex appears to have therapeutic effects on the functional recovery and anatomic reconstruction of the adult rats with spinal cord injury. 展开更多
关键词 Spinal cord injuries Tissue engineering Stem cell transplantation Polyglycolic acid
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Repair effect of Schwann cells modified by microgene pSVPoMcat on injured spinal cord in rats 被引量:7
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作者 陈礼刚 高立达 +4 位作者 卢敏 毛伯镛 曾凡俊 李开慧 朴永旭 《Chinese Journal of Traumatology》 CAS 2002年第4期241-245,共5页
Objective: To observe the repair effect of Schwann cells (SCs) modified by microgene pSVPoMcat on injured spinal cord in rats. Methods: Semi transection injury at the level of T 8 of spinal cord was made with cutting ... Objective: To observe the repair effect of Schwann cells (SCs) modified by microgene pSVPoMcat on injured spinal cord in rats. Methods: Semi transection injury at the level of T 8 of spinal cord was made with cutting method on 120 Sprague Dawley (SD) rats. Then 40 rats implanted with SCs modified by microgene pSVPoMcat were taken as Group A, 40 rats implanted with simple SCs as Group B and the other 40 rats were taken as the control group (Group C). The functional recovery of the rats was observed through combined behavioral score (CBS) and cortical somatosensory evoked potential (CSEP), and the expression of the glial fibrillary acidic protein (GFAP) was measured with in situ hybridization and immunocytochemistry. At 3 months after operation, the rats were examined with magnetic resonance image (MRI), and the neurofilaments (NF) of the axons were stained with immunohistochemical method. Results: GFAP expression in Group A was significantly lower than that of the other 2 groups. MRI showed that the spinal signals in the injured area recovered fundamentally in Group A, didnt recover in Group B and malacia focus was found in Group C, which was same as the results of NF staining. Wave amplitudes in incubation periods in Group A and Group B tended to recover. It recovered to the normal level in Group A, which was similar to the results of CBS.Conclusions: SCs modified by microgene pSVPoMcat can inhibit GFAP expression, improve the growth of the axons and the functional recovery of neurons after spinal cord injury. 展开更多
关键词 Analysis of Variance Animals Evoked Potentials Somatosensory Gene Therapy Gene Transfer Techniques Glial Fibrillary Acidic Protein Immunohistochemistry In Situ Hybridization Magnetic Resonance Imaging Nerve Regeneration RATS Rats Sprague-Dawley Schwann Cells Spinal Cord Spinal Cord Injuries
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