A green-revertible albino mutant-Qiufeng M was found from the japonica rice （Oryza sativa L. ssp. japonica） Qiufeng in the field. The first three leaves of the mutant were albino with some green. The leaf color beca...A green-revertible albino mutant-Qiufeng M was found from the japonica rice （Oryza sativa L. ssp. japonica） Qiufeng in the field. The first three leaves of the mutant were albino with some green. The leaf color became pale green since the fourth leaf and the glume had the same phenomenon as the first three leaves. The measuring data of the pigment content confirmed the visually observed results. It truly had a remarkable changing process in the leaf color in Qiufeng M. Comparison of the main agronomic characters between Qiufeng and Qiufeng M indicated that the neck length and grain weight showed significant difference at the 1% level, and other characters were not different. Genetic analysis showed that the green-revertible albino trait was controlled by a single recessive nucleic gene. Using 209 recessive mutant individuals in the F2 population derived from the cross Pei＇ai 64S × Qiufeng M, a gene, tentatively named gra（t）, was located between the SSR markers of RM475 and RM2-22 on the long arm of chromosome 2. The genetic distance were 17.3 cM and 2.9 cM respectively.展开更多
Rail fastener positioning method has high requirements for image quality and positioning accuracy.Therefore,a rail fastener positioning method based on gray mutation is proposed.Firstly,the image is denoised by an imp...Rail fastener positioning method has high requirements for image quality and positioning accuracy.Therefore,a rail fastener positioning method based on gray mutation is proposed.Firstly,the image is denoised by an improved median filter.Then,according to the characteristics of image frequency domain,the image is decomposed by wavelet transfrom to extract low-frequency component,and the low-frequency component is filtered and processed by gamma transformation to reduce the influence of natural environment factors on image quality.After that,according to the change rule of gray-scale values of different regions in the image,the gray-scale mutation in column and line directions of the image is statistically analyzed,and then the rail surface and the sleeper are located.Finally,the fastener area is accurately located by using the position relationship of the rail surface,the sleeper and the fastener in the image.The experimental results show that the positioning accuracy of the proposed method is 93.19%,which can quickly and effectively locate the fastener region,and has strong environmental adaptability,robustness and practicability.展开更多
Intact pZ189 DNA was replicated in monkey kidney vero cells which had been pretreated with Nmethyl-N’-nitro-N-nitrosoguanidine (MNNG) . The mutants were selected in E. coli MBM7070 and the mutation frequencies involv...Intact pZ189 DNA was replicated in monkey kidney vero cells which had been pretreated with Nmethyl-N’-nitro-N-nitrosoguanidine (MNNG) . The mutants were selected in E. coli MBM7070 and the mutation frequencies involving mutants with unchanged electrophoretic mobilrty of their plasmid DNA were scored. When compared to the spontaneous mutation frequency. the mutation frequencies were increased by 5.8 and 2.9-fold in cells pretreated with 0. 2 and 2μmol/L MNNG, respectively. The supF genes of these mutants were sequenced. and it was found that the types of base substitution and the sites of frameshifts differed from findings in studies of spontaneous and targeted mutagenesis. The results suggest that nontargeted mutagenesis occurs in mammalian cells and may have a sequence specificity.展开更多
基金This work was supported by the Major Research Program on Technology of Agricultural Structure Adjustment (No. 05-01-05B)Jiangsu High Technology Program (No. BG2004301, BG2004304, and BG2005301).
文摘A green-revertible albino mutant-Qiufeng M was found from the japonica rice （Oryza sativa L. ssp. japonica） Qiufeng in the field. The first three leaves of the mutant were albino with some green. The leaf color became pale green since the fourth leaf and the glume had the same phenomenon as the first three leaves. The measuring data of the pigment content confirmed the visually observed results. It truly had a remarkable changing process in the leaf color in Qiufeng M. Comparison of the main agronomic characters between Qiufeng and Qiufeng M indicated that the neck length and grain weight showed significant difference at the 1% level, and other characters were not different. Genetic analysis showed that the green-revertible albino trait was controlled by a single recessive nucleic gene. Using 209 recessive mutant individuals in the F2 population derived from the cross Pei＇ai 64S × Qiufeng M, a gene, tentatively named gra（t）, was located between the SSR markers of RM475 and RM2-22 on the long arm of chromosome 2. The genetic distance were 17.3 cM and 2.9 cM respectively.
基金National Natural Science Foundation of China(Nos.61616202,61461203)Ministry of Education Innovation Team Development Plan(No.IRT_16R36)Plateau Information Engineering and Control Key Practice Laboratory Open Project Fund of Gansu Province(No.201611105)。
文摘Rail fastener positioning method has high requirements for image quality and positioning accuracy.Therefore,a rail fastener positioning method based on gray mutation is proposed.Firstly,the image is denoised by an improved median filter.Then,according to the characteristics of image frequency domain,the image is decomposed by wavelet transfrom to extract low-frequency component,and the low-frequency component is filtered and processed by gamma transformation to reduce the influence of natural environment factors on image quality.After that,according to the change rule of gray-scale values of different regions in the image,the gray-scale mutation in column and line directions of the image is statistically analyzed,and then the rail surface and the sleeper are located.Finally,the fastener area is accurately located by using the position relationship of the rail surface,the sleeper and the fastener in the image.The experimental results show that the positioning accuracy of the proposed method is 93.19%,which can quickly and effectively locate the fastener region,and has strong environmental adaptability,robustness and practicability.
文摘Intact pZ189 DNA was replicated in monkey kidney vero cells which had been pretreated with Nmethyl-N’-nitro-N-nitrosoguanidine (MNNG) . The mutants were selected in E. coli MBM7070 and the mutation frequencies involving mutants with unchanged electrophoretic mobilrty of their plasmid DNA were scored. When compared to the spontaneous mutation frequency. the mutation frequencies were increased by 5.8 and 2.9-fold in cells pretreated with 0. 2 and 2μmol/L MNNG, respectively. The supF genes of these mutants were sequenced. and it was found that the types of base substitution and the sites of frameshifts differed from findings in studies of spontaneous and targeted mutagenesis. The results suggest that nontargeted mutagenesis occurs in mammalian cells and may have a sequence specificity.
