目的了解不同血清群致病性钩体的脉冲场凝胶电泳(pulsed-field gel electrophoresis,PFGE)带型特征。方法应用PFGE方法对65株不同血清群致病性钩体国际、国内参考菌株和疫苗株进行分型,并以此聚类分析,同时与菌株的多位点序列分型(multi...目的了解不同血清群致病性钩体的脉冲场凝胶电泳(pulsed-field gel electrophoresis,PFGE)带型特征。方法应用PFGE方法对65株不同血清群致病性钩体国际、国内参考菌株和疫苗株进行分型,并以此聚类分析,同时与菌株的多位点序列分型(multilocus sequence typing,MLST)、多位点串联重复序列数变化分析(multilocus VNTR analysis,MLVA)分型结果进行比较研究。结果不同血清群致病性钩体基因组DNA经NotⅠ酶切电泳后,获得了较高清晰度酶切图谱,各条带分离良好。65株钩体菌株分为61种PFGE型别。相同的血清群的钩体菌株的PFGE型别不尽相同。比较分析结果显示尽管PFGE、MLST和MLVA之间的分型结果不尽相同,但都具有较高的分辨率。聚类分析显示这些菌株可形成11个大小不一进化簇,呈现多点分散进化特征。结论获得致病性钩体分子分型的第一手资料,为后续钩体分子溯源及流行病学研究提供科学指导。展开更多
Phase diverse speckle is a novel kind of imaging technique and can be used to overcome image degradation from unknown phase aberrations, such as atmospheric turbulence. The wave-front phase expanded on the Zernike pol...Phase diverse speckle is a novel kind of imaging technique and can be used to overcome image degradation from unknown phase aberrations, such as atmospheric turbulence. The wave-front phase expanded on the Zernike polynomials is esti- mated from a pair of images (in the focal and out of focus planes). In this paper the principle of PDS is analyzed, and genetic algorithm is used as the iterative algorithm to simulate some characteristics, such as the influence of Zernike polynomials’ mode, amplitude of turbulence on the phase estimation. Thus, a new method for recovery of images is explored.展开更多
Petermann Glacier is a marine-terminating outlet glacier that had a 70 km-long floating ice tongue prior to a ～ 270 km2 calving event that was observed from satellite sensors in August 2010,shortening the ice tongue ...Petermann Glacier is a marine-terminating outlet glacier that had a 70 km-long floating ice tongue prior to a ～ 270 km2 calving event that was observed from satellite sensors in August 2010,shortening the ice tongue by ～ 27 km.Further,in July 2012,another 10 km was lost through calving.In order to understand these events in perspective,here the authors perform a long-term data analysis of Petermann Glacier calving-front variability and ice velocity for each year in the 1990s-2000s,supplemented by available observations from the previous three decades.Five major （on the order of 100 krm2） calving events are identified,with ～ 153 km2 calved from 1959 to 1961,～ 168 km2 in 1991,～ 71 km2 in 2001,～ 270 km2 in 2010,and ～ 130 km2 in 2012-as well as ～ 31 k m2 calved in 2008.The increased frequency of major calving events in recent years has left the front terminus position retreated nearly 25 km beyond the range of observed in previous decades.In contrast,stable ice-dynamics are suggested from ice-velocity measurements made each year between 1993-2012,which are on average 1063 m yr-1,with limited interannual variability and no significant trend; moreover,there is no apparent relationship between ice-velocity variability and calving events.The degree to which the massive calving events in 2010 and 2012 represent natural episodic variability or a response to atmospheric and/or oceanic changes remains speculative; however,melt-induced weakening of the floating ice tongue in recent years is strongly suggested.展开更多
No Con A receptor site was found on the intact plasma membranes of mammalian sperms before capacitation.After capacitation, however, the plasma membranes sloughed off, exposing the outer acrosomal membrane which is ri...No Con A receptor site was found on the intact plasma membranes of mammalian sperms before capacitation.After capacitation, however, the plasma membranes sloughed off, exposing the outer acrosomal membrane which is rich in Con A receptor sites.The vesicles formed during acrosome reaction were also found to bc rich in Con A receptor sites, suggesting their origin from outer acrosomal membranes.With completion of acrosome reaction, only inner acrosomal membrane was left in which no Con A receptor sites could be demonstrated.Also no Con A receptor site was found on egg plasma membrane throughout fertilization.It was thus shown that different membranes possess different properties.展开更多
The contact melting of phase change material around a moving horizontal cylindrical heat source, which descended under its own weight, is investigated in this article. A melting model under constant surface heat flux ...The contact melting of phase change material around a moving horizontal cylindrical heat source, which descended under its own weight, is investigated in this article. A melting model under constant surface heat flux is established. The analytical results for thickness and pressure distributions inside melt layer and steady melting velocity are obtained by using contact melting theory. The melting law is discussed, and compared with that of contact melting driven by temperature difference. It is found that quasi-steady melting velocity is determined by heat flux of heat source, and the variation of heat source density has less effect on melting velocity.展开更多
The origin of power-law distributions in self-organized criticality is investigated by treating the variation of the number of active sites in the system as a stochastic process. An avalanche is mapped to a first-retu...The origin of power-law distributions in self-organized criticality is investigated by treating the variation of the number of active sites in the system as a stochastic process. An avalanche is mapped to a first-return random-walk process in a one-dimensional lattice. In order to understand the reason of variant exponents for the power-law distributions in different self-organized critical systems, we introduce the correlations among evolution steps. Power-law distributions of the lifetime and spatial size are found when the random walk is unbiased with equal probability to move in opposite directions. It is found that the longer the correlation length, the smaller values of the exponents for the power-law distributions.展开更多
文摘Phase diverse speckle is a novel kind of imaging technique and can be used to overcome image degradation from unknown phase aberrations, such as atmospheric turbulence. The wave-front phase expanded on the Zernike polynomials is esti- mated from a pair of images (in the focal and out of focus planes). In this paper the principle of PDS is analyzed, and genetic algorithm is used as the iterative algorithm to simulate some characteristics, such as the influence of Zernike polynomials’ mode, amplitude of turbulence on the phase estimation. Thus, a new method for recovery of images is explored.
基金supported by the Trond Mohn Donation to the Mohn-Sverdrup Center at the Nansen Center,the Research Council of Norway’s UK/Netherlands/Norway RAPID programthe project AWAKE under the Polish-Norwegian Research Fund(2009–2011)
文摘Petermann Glacier is a marine-terminating outlet glacier that had a 70 km-long floating ice tongue prior to a ～ 270 km2 calving event that was observed from satellite sensors in August 2010,shortening the ice tongue by ～ 27 km.Further,in July 2012,another 10 km was lost through calving.In order to understand these events in perspective,here the authors perform a long-term data analysis of Petermann Glacier calving-front variability and ice velocity for each year in the 1990s-2000s,supplemented by available observations from the previous three decades.Five major （on the order of 100 krm2） calving events are identified,with ～ 153 km2 calved from 1959 to 1961,～ 168 km2 in 1991,～ 71 km2 in 2001,～ 270 km2 in 2010,and ～ 130 km2 in 2012-as well as ～ 31 k m2 calved in 2008.The increased frequency of major calving events in recent years has left the front terminus position retreated nearly 25 km beyond the range of observed in previous decades.In contrast,stable ice-dynamics are suggested from ice-velocity measurements made each year between 1993-2012,which are on average 1063 m yr-1,with limited interannual variability and no significant trend; moreover,there is no apparent relationship between ice-velocity variability and calving events.The degree to which the massive calving events in 2010 and 2012 represent natural episodic variability or a response to atmospheric and/or oceanic changes remains speculative; however,melt-induced weakening of the floating ice tongue in recent years is strongly suggested.
文摘No Con A receptor site was found on the intact plasma membranes of mammalian sperms before capacitation.After capacitation, however, the plasma membranes sloughed off, exposing the outer acrosomal membrane which is rich in Con A receptor sites.The vesicles formed during acrosome reaction were also found to bc rich in Con A receptor sites, suggesting their origin from outer acrosomal membranes.With completion of acrosome reaction, only inner acrosomal membrane was left in which no Con A receptor sites could be demonstrated.Also no Con A receptor site was found on egg plasma membrane throughout fertilization.It was thus shown that different membranes possess different properties.
基金the National Natural Science Foundation of China Project No.50376074
文摘The contact melting of phase change material around a moving horizontal cylindrical heat source, which descended under its own weight, is investigated in this article. A melting model under constant surface heat flux is established. The analytical results for thickness and pressure distributions inside melt layer and steady melting velocity are obtained by using contact melting theory. The melting law is discussed, and compared with that of contact melting driven by temperature difference. It is found that quasi-steady melting velocity is determined by heat flux of heat source, and the variation of heat source density has less effect on melting velocity.
基金Supported in part by the National Natural Science Foundation of China under Grant Nos.10635020 and 10775057by the Ministry of Education of China under Grant Nos.306022,IRT0624by the Programme of Introducing Talents of Discipline to Universities under Grant No.B08033
文摘The origin of power-law distributions in self-organized criticality is investigated by treating the variation of the number of active sites in the system as a stochastic process. An avalanche is mapped to a first-return random-walk process in a one-dimensional lattice. In order to understand the reason of variant exponents for the power-law distributions in different self-organized critical systems, we introduce the correlations among evolution steps. Power-law distributions of the lifetime and spatial size are found when the random walk is unbiased with equal probability to move in opposite directions. It is found that the longer the correlation length, the smaller values of the exponents for the power-law distributions.
