以谷子(Setaria italica Beauv.)抗锈病植株十里香和感锈病植株豫谷1号为材料,利用抗病基因同源序列(RGA)技术克隆谷子核苷酸结合位点(NBS)类型RGA并进行分析。共获得了3个RGA,分别命名为RUS1-1、RUS1-2、RUS1-3(Resistance against Uro...以谷子(Setaria italica Beauv.)抗锈病植株十里香和感锈病植株豫谷1号为材料,利用抗病基因同源序列(RGA)技术克隆谷子核苷酸结合位点(NBS)类型RGA并进行分析。共获得了3个RGA,分别命名为RUS1-1、RUS1-2、RUS1-3(Resistance against Uromyces setariae-italicae,RUS),它们编码的蛋白质均含有NBS类型抗病基因编码蛋白的共有特征结构域P-loop和Kinase2α。BLASTX分析结果表明,3个RGA的编码蛋白与水稻中含有NB-ARC信号传导结构域的蛋白质、NBS-LRR类型抗病基因等的编码蛋白同源性为47%～66%。聚类分析结果表明,3个RGA属于NBS-LRR类型抗病基因,且与番茄NBS-LRR类型抗病基因I2聚为一类。展开更多
Traditionally, the multibasic cleavage site （MBCS） of surface protein H5-hemagglutinin （HA） is converted to a monobasic one so as to weaken the virulence of recombinant H5N1 influenza viruses and to produce inacti...Traditionally, the multibasic cleavage site （MBCS） of surface protein H5-hemagglutinin （HA） is converted to a monobasic one so as to weaken the virulence of recombinant H5N1 influenza viruses and to produce inactivated and live attenuated vaccines. Whether such modification benefits new candidate vaccines has not been adequately investigated. We previously used retroviral vectors to generate wtH5N1 pseudotypes containing the wild-type HA （wtH5） from A/swine/Anhui/ca/2004 （H5N1） virus. Here, we generated mtH5N1 pseudotypes, which contained a mutant-type HA （mtH5） with a modified monobasic cleavage site. Groups of mice were subcutaneously injected with the two types of influenza pseudotypes. Compared to the group immunized with wtH5N1 pseudotypes, the inoculation of mtH5N1 pseudotypes induced significantly higher levels of HA specific IgG and IFN-y in immunized mice, and enhanced protection against the challenge of mouse-adapted avian influenza virus A/Chicken/Henardl2/2004 （H5N1）. This study suggests modification of the H5-hemagglutinin MBCS in retroviral pseudotypes enhances protection efficacy in mice and this information may be helpful for development of vaccines from mammalian cells to fight against H5N 1 influenza viruses.展开更多
In wireless sensor networks,node localization is a fundamental middleware service.In this paper,a robust and accurate localization algorithm is proposed,which uses a novel iterative clustering model to obtain the most...In wireless sensor networks,node localization is a fundamental middleware service.In this paper,a robust and accurate localization algorithm is proposed,which uses a novel iterative clustering model to obtain the most representative intersection points between every two circles and use them to estimate the position of unknown nodes.Simulation results demonstrate that the proposed algorithm outperforms other localization schemes (such as Min-Max,etc.) in accuracy,scalability and gross error tolerance.展开更多
文摘以谷子(Setaria italica Beauv.)抗锈病植株十里香和感锈病植株豫谷1号为材料,利用抗病基因同源序列(RGA)技术克隆谷子核苷酸结合位点(NBS)类型RGA并进行分析。共获得了3个RGA,分别命名为RUS1-1、RUS1-2、RUS1-3(Resistance against Uromyces setariae-italicae,RUS),它们编码的蛋白质均含有NBS类型抗病基因编码蛋白的共有特征结构域P-loop和Kinase2α。BLASTX分析结果表明,3个RGA的编码蛋白与水稻中含有NB-ARC信号传导结构域的蛋白质、NBS-LRR类型抗病基因等的编码蛋白同源性为47%～66%。聚类分析结果表明,3个RGA属于NBS-LRR类型抗病基因,且与番茄NBS-LRR类型抗病基因I2聚为一类。
基金supported by the National Basic Research Program of China (973: 2012CB518904) from the Ministry of Science and Technology of Chinathe National Natural Science Foundation of China(81201298)
文摘Traditionally, the multibasic cleavage site （MBCS） of surface protein H5-hemagglutinin （HA） is converted to a monobasic one so as to weaken the virulence of recombinant H5N1 influenza viruses and to produce inactivated and live attenuated vaccines. Whether such modification benefits new candidate vaccines has not been adequately investigated. We previously used retroviral vectors to generate wtH5N1 pseudotypes containing the wild-type HA （wtH5） from A/swine/Anhui/ca/2004 （H5N1） virus. Here, we generated mtH5N1 pseudotypes, which contained a mutant-type HA （mtH5） with a modified monobasic cleavage site. Groups of mice were subcutaneously injected with the two types of influenza pseudotypes. Compared to the group immunized with wtH5N1 pseudotypes, the inoculation of mtH5N1 pseudotypes induced significantly higher levels of HA specific IgG and IFN-y in immunized mice, and enhanced protection against the challenge of mouse-adapted avian influenza virus A/Chicken/Henardl2/2004 （H5N1）. This study suggests modification of the H5-hemagglutinin MBCS in retroviral pseudotypes enhances protection efficacy in mice and this information may be helpful for development of vaccines from mammalian cells to fight against H5N 1 influenza viruses.
基金supported in part by the Key Program of National Natural Science Foundation of China(Grant No.60873244,60973110,61003307)the Beijing Municipal Natural Science Foundation(Grant No.4102059)
文摘In wireless sensor networks,node localization is a fundamental middleware service.In this paper,a robust and accurate localization algorithm is proposed,which uses a novel iterative clustering model to obtain the most representative intersection points between every two circles and use them to estimate the position of unknown nodes.Simulation results demonstrate that the proposed algorithm outperforms other localization schemes (such as Min-Max,etc.) in accuracy,scalability and gross error tolerance.
