Geosynthetic-reinforced and pile-supported (GRPS) embankment has been increasingly constructed in a large number of regions and for a wide range of projects in the past decades. However, many disadvantages are expos...Geosynthetic-reinforced and pile-supported (GRPS) embankment has been increasingly constructed in a large number of regions and for a wide range of projects in the past decades. However, many disadvantages are exposed through a lot of applications on conventional technique of GRPS embankment (called CT embankment), i.e., intolerable settlement and lateral displacement, low geosynthetic efficiency, etc. In view of these disadvantages, the fixed geosynthetic technique of GRPS embankment (called FGT embankment) is developed in this work. In this system, the geosynthetic is fixed on the pile head by the steel bar fulcrum and concrete fixed top. The principles and construction techniques involved in the FGT embankment are described firstly. Then, the numerical analysis method and two-stage analysis method are used to study the performance of FGT embankment, respectively. It is shown that the FGT embankment can provide a better improvement technique to construct a high embankment over soft ground.展开更多
Based on back analysis of lateral displacements measured in situ by using the analytical solution, a useful method for estimating stress concentration ratio of geosynthetic-reinforced and pile-supported(GRPS) embankme...Based on back analysis of lateral displacements measured in situ by using the analytical solution, a useful method for estimating stress concentration ratio of geosynthetic-reinforced and pile-supported(GRPS) embankments was proposed. In order to validate the proposed method, a full-scale high-speed railway embankment(HSRE) with four instrumented subsections over medium compressibility silty clay was constructed in three stages. The soil profile, construction procedure and monitoring of settlements and lateral displacements of the four test sections were described. The field deformation analysis results show that 1) the combined reinforcement of CFG piles and geosynthetic layer perform well in terms of reducing lateral displacements; 2) the development of lateral displacements lags behind the increase of fill load, which can be attributed to the vertical load transfer mechanism of the pile foundation; and 3) pile length has a dominant effect on the stress distribution proportion between piles and surrounding soils. The comparison between predicted and experimental results suggests that the proposed analytical solution and the back analysis-based method are capable of reasonably estimating the lateral deformation and the stress concentration ratio, respectively, if the appropriate soil elastic modulus is chosen.展开更多
We have synthesized water-stable polyaniline nanoparticles coated with tri- armed polyethylene glycol chains using a solvent-shift method and confirmed their colloidal size and aqueous solubility. Furthermore, we have...We have synthesized water-stable polyaniline nanoparticles coated with tri- armed polyethylene glycol chains using a solvent-shift method and confirmed their colloidal size and aqueous solubility. Furthermore, we have demonstrated that the polyaniline nanoparticles can be doped with biological dopants to produce distinct color changes allowing the detection of live cancer cells.展开更多
Euryarchaeota and Crenarchaeota, the two main lineages of the domain Archaea, encode different chromatin proteins and differ in the use of replicative DNA polymerases. Crenarchaea possess a single family B DNA polymer...Euryarchaeota and Crenarchaeota, the two main lineages of the domain Archaea, encode different chromatin proteins and differ in the use of replicative DNA polymerases. Crenarchaea possess a single family B DNA polymerase(Pol B), which is capable of strand displacement modulated by the chromatin proteins Cren7 and Sul7 d. Euryarchaea have two distinct replicative DNA polymerases, PolB and PolD, a family D DNA polymerase. Here we characterized the strand displacement activities of Pol B and Pol D from the hyperthermophilic euryarchaeon Pyrococcus furiosus and investigated the influence of HPf A1, a homolog of eukaryotic histones from P. furiosus, on these activities. We showed that both Pol B and Pol D were efficient in strand displacement. HPf A1 inhibited DNA strand displacement by both DNA polymerases but exhibited little effect on the displacement of a RNA strand annealed to single-stranded template DNA. This is consistent with the finding that HPf A1 bound more tightly to double-stranded DNA than to a RNA:DNA hybrid. Our results suggest that, although crenarchaea and euryarchaea differ in chromosomal packaging, they share similar mechanisms in modulating strand displacement by DNA polymerases during lagging strand DNA synthesis.展开更多
基金Foundation item: Project(51278216) supported by the National Natural Science Foundation of China Project(11-2-05) supported by the Scientific and Technological Project for Shanxi Communication Construction, China Project(HF-08-01-2011-240) supported by the Graduates' Innovation Fund of Huazhong University of Science and Technology, China
文摘Geosynthetic-reinforced and pile-supported (GRPS) embankment has been increasingly constructed in a large number of regions and for a wide range of projects in the past decades. However, many disadvantages are exposed through a lot of applications on conventional technique of GRPS embankment (called CT embankment), i.e., intolerable settlement and lateral displacement, low geosynthetic efficiency, etc. In view of these disadvantages, the fixed geosynthetic technique of GRPS embankment (called FGT embankment) is developed in this work. In this system, the geosynthetic is fixed on the pile head by the steel bar fulcrum and concrete fixed top. The principles and construction techniques involved in the FGT embankment are described firstly. Then, the numerical analysis method and two-stage analysis method are used to study the performance of FGT embankment, respectively. It is shown that the FGT embankment can provide a better improvement technique to construct a high embankment over soft ground.
基金Project(2010G003-F)supported by Technological Research and Development Programs of the Ministry of Railways,China
文摘Based on back analysis of lateral displacements measured in situ by using the analytical solution, a useful method for estimating stress concentration ratio of geosynthetic-reinforced and pile-supported(GRPS) embankments was proposed. In order to validate the proposed method, a full-scale high-speed railway embankment(HSRE) with four instrumented subsections over medium compressibility silty clay was constructed in three stages. The soil profile, construction procedure and monitoring of settlements and lateral displacements of the four test sections were described. The field deformation analysis results show that 1) the combined reinforcement of CFG piles and geosynthetic layer perform well in terms of reducing lateral displacements; 2) the development of lateral displacements lags behind the increase of fill load, which can be attributed to the vertical load transfer mechanism of the pile foundation; and 3) pile length has a dominant effect on the stress distribution proportion between piles and surrounding soils. The comparison between predicted and experimental results suggests that the proposed analytical solution and the back analysis-based method are capable of reasonably estimating the lateral deformation and the stress concentration ratio, respectively, if the appropriate soil elastic modulus is chosen.
文摘We have synthesized water-stable polyaniline nanoparticles coated with tri- armed polyethylene glycol chains using a solvent-shift method and confirmed their colloidal size and aqueous solubility. Furthermore, we have demonstrated that the polyaniline nanoparticles can be doped with biological dopants to produce distinct color changes allowing the detection of live cancer cells.
基金supported by the National Natural Science Foundation of China (31130003, 30921065)
文摘Euryarchaeota and Crenarchaeota, the two main lineages of the domain Archaea, encode different chromatin proteins and differ in the use of replicative DNA polymerases. Crenarchaea possess a single family B DNA polymerase(Pol B), which is capable of strand displacement modulated by the chromatin proteins Cren7 and Sul7 d. Euryarchaea have two distinct replicative DNA polymerases, PolB and PolD, a family D DNA polymerase. Here we characterized the strand displacement activities of Pol B and Pol D from the hyperthermophilic euryarchaeon Pyrococcus furiosus and investigated the influence of HPf A1, a homolog of eukaryotic histones from P. furiosus, on these activities. We showed that both Pol B and Pol D were efficient in strand displacement. HPf A1 inhibited DNA strand displacement by both DNA polymerases but exhibited little effect on the displacement of a RNA strand annealed to single-stranded template DNA. This is consistent with the finding that HPf A1 bound more tightly to double-stranded DNA than to a RNA:DNA hybrid. Our results suggest that, although crenarchaea and euryarchaea differ in chromosomal packaging, they share similar mechanisms in modulating strand displacement by DNA polymerases during lagging strand DNA synthesis.