The pollen of two tomato varieties, Ryau961721 and Ryau9327D, was adopted in our research. The two tomato varieties were bred by College of Land- scape and Horticulture, Yunnan Agricultural University. The collected p...The pollen of two tomato varieties, Ryau961721 and Ryau9327D, was adopted in our research. The two tomato varieties were bred by College of Land- scape and Horticulture, Yunnan Agricultural University. The collected pollen was stored in low-temperature （4 ℃） and ultra-low-temperature （-196 ℃） circumstances. Then it was inoculated to the medium and cultured at 28 ℃ in thermostat incubator. The pollen viability was determined by electron microscope. The results showed that compared to that of pollen stored in control （25 ℃） circumstance, the viability of pollen stored in low-temperature （4 ℃） and ultra-low-temperature （-196 ℃） circum- stances for 1 -3 d did not change significantly. In addition, pollen viability trended to decrease with the increase of freeze-thaw cycle and storage time. The pollen lost basically the viability by the 7th d in the storage.展开更多
基金Supported by College Students’Technology Innovation and Entrepreneurship Action Fund of Yunnan Agricultural University(2014ZKX048)~~
文摘The pollen of two tomato varieties, Ryau961721 and Ryau9327D, was adopted in our research. The two tomato varieties were bred by College of Land- scape and Horticulture, Yunnan Agricultural University. The collected pollen was stored in low-temperature （4 ℃） and ultra-low-temperature （-196 ℃） circumstances. Then it was inoculated to the medium and cultured at 28 ℃ in thermostat incubator. The pollen viability was determined by electron microscope. The results showed that compared to that of pollen stored in control （25 ℃） circumstance, the viability of pollen stored in low-temperature （4 ℃） and ultra-low-temperature （-196 ℃） circum- stances for 1 -3 d did not change significantly. In addition, pollen viability trended to decrease with the increase of freeze-thaw cycle and storage time. The pollen lost basically the viability by the 7th d in the storage.
