Tobacco mosaic virus(TMV) causes significant yield loss in susceptible crops irrigated with contaminated water. However, detection of TMV in water is difficult owing to extremely low concentrations of the virus. Here,...Tobacco mosaic virus(TMV) causes significant yield loss in susceptible crops irrigated with contaminated water. However, detection of TMV in water is difficult owing to extremely low concentrations of the virus. Here, we developed a simple method for the detection and quantification of TMV in irrigation water. TMV was reliably detected at concentrations as low as 10 viral copies/μL with real-time PCR. The sensitivity of detection was further improved using polyethylene glycol 6000(PEG6000, MW 6000) to concentrate TMV from water samples. Among the 28 samples from Shaanxi Province examined with our method, 17 were tested positive after virus concentration. Infectivity of TMV in the original water sample as well as after concentration was confirmed using PCR. The limiting concentration of TMV in water to re-infect plants was determined as 102 viral copies/mL. The method developed in this study offers a novel approach to detect TMV in irrigation water, and may provide an effective tool to control crop infection.展开更多
The study examined the impact of using power tiller as a means of mechanizing lowland rice production in Nigeria. The study was carried out in Bida area, Niger State, where the sawah rice production was disseminated b...The study examined the impact of using power tiller as a means of mechanizing lowland rice production in Nigeria. The study was carried out in Bida area, Niger State, where the sawah rice production was disseminated by Watershed Initiative in Nigeria （WIN）. And in Ajase Ipo of Kwara State, some of the parameters assessed during the study included average speed of operation, average wheel slip/travel reduction, average draught of implement, and fuel consumption. The cost of operation and yield over five years of usage at Niger State and two years in Kwara State was determined and it was therefore concluded that the power tiller is the most appropriate field machinery for tillage operations such as puddling, leveling on lowland rice production in Nigeria.展开更多
基金supported by the Key Technology Program of China National Tobacco Corporation (110200902046)111 Project from the Education Ministry of China, (No.B07049)the National High-tech R&D Program of China (no. 2012AA101504)
文摘Tobacco mosaic virus(TMV) causes significant yield loss in susceptible crops irrigated with contaminated water. However, detection of TMV in water is difficult owing to extremely low concentrations of the virus. Here, we developed a simple method for the detection and quantification of TMV in irrigation water. TMV was reliably detected at concentrations as low as 10 viral copies/μL with real-time PCR. The sensitivity of detection was further improved using polyethylene glycol 6000(PEG6000, MW 6000) to concentrate TMV from water samples. Among the 28 samples from Shaanxi Province examined with our method, 17 were tested positive after virus concentration. Infectivity of TMV in the original water sample as well as after concentration was confirmed using PCR. The limiting concentration of TMV in water to re-infect plants was determined as 102 viral copies/mL. The method developed in this study offers a novel approach to detect TMV in irrigation water, and may provide an effective tool to control crop infection.
文摘The study examined the impact of using power tiller as a means of mechanizing lowland rice production in Nigeria. The study was carried out in Bida area, Niger State, where the sawah rice production was disseminated by Watershed Initiative in Nigeria （WIN）. And in Ajase Ipo of Kwara State, some of the parameters assessed during the study included average speed of operation, average wheel slip/travel reduction, average draught of implement, and fuel consumption. The cost of operation and yield over five years of usage at Niger State and two years in Kwara State was determined and it was therefore concluded that the power tiller is the most appropriate field machinery for tillage operations such as puddling, leveling on lowland rice production in Nigeria.
