基于A型和C型鸭甲肝病毒(Duck hepatitis A virus,DHAV)3D基因的6个保守区域设计了4条引物,利用BstDNA聚合酶在60℃恒温保持45min即可完成反转录和扩增反应,由此建立了鸭甲肝病毒的一步反转录环介导等温扩增(RT-LAMP)方法。该方法具有...基于A型和C型鸭甲肝病毒(Duck hepatitis A virus,DHAV)3D基因的6个保守区域设计了4条引物,利用BstDNA聚合酶在60℃恒温保持45min即可完成反转录和扩增反应,由此建立了鸭甲肝病毒的一步反转录环介导等温扩增(RT-LAMP)方法。该方法具有良好的特异性,除DHAV外,对其他4种常见鸭病的检测结果均为阴性。该方法对DHAV-A和DHAV-C病毒RNA的最低检出量均为0.1pg,是常规RT-PCR方法的10倍。临床应用结果表明,该方法与病毒分离鉴定方法的符合率为92.5%,而且对仪器要求低,适于基层实验室和现场检测。展开更多
In order to meet the progressive requirement for the performance improvement of steel,the author proposed a novel microstructure featured with multi-phase,meta-stable and multi-scale(so-called as M 3).And then,the new...In order to meet the progressive requirement for the performance improvement of steel,the author proposed a novel microstructure featured with multi-phase,meta-stable and multi-scale(so-called as M 3).And then,the new technologies could be developed to process three prototype steels with high performance:the third generation high strength low alloy(HSLA) steels with improved toughness and/or ductility(AKV(40℃)≥200 J and/or A≥20% when Rp0.2 in 800-1000 MPa),the third generation advanced high strength steels(AHSS)(Rm×A≥30 GPa% when Rm from 1000 MPa to 1500 MPa) for automobiles with improved ductility and low cost,and heat resistant martensitic steels with improved creep strength(10000650≥90 MPa).It can be expected that the new technology developed will remarkably improve the safety and reliability of steel products in service for infrastructures,automobiles and fossil power station in the future.展开更多
文摘基于A型和C型鸭甲肝病毒(Duck hepatitis A virus,DHAV)3D基因的6个保守区域设计了4条引物,利用BstDNA聚合酶在60℃恒温保持45min即可完成反转录和扩增反应,由此建立了鸭甲肝病毒的一步反转录环介导等温扩增(RT-LAMP)方法。该方法具有良好的特异性,除DHAV外,对其他4种常见鸭病的检测结果均为阴性。该方法对DHAV-A和DHAV-C病毒RNA的最低检出量均为0.1pg,是常规RT-PCR方法的10倍。临床应用结果表明,该方法与病毒分离鉴定方法的符合率为92.5%,而且对仪器要求低,适于基层实验室和现场检测。
基金Ministry of Science and Technology is acknowledged for the financial funding of the"973 program" of Grant No. 2010CB630800
文摘In order to meet the progressive requirement for the performance improvement of steel,the author proposed a novel microstructure featured with multi-phase,meta-stable and multi-scale(so-called as M 3).And then,the new technologies could be developed to process three prototype steels with high performance:the third generation high strength low alloy(HSLA) steels with improved toughness and/or ductility(AKV(40℃)≥200 J and/or A≥20% when Rp0.2 in 800-1000 MPa),the third generation advanced high strength steels(AHSS)(Rm×A≥30 GPa% when Rm from 1000 MPa to 1500 MPa) for automobiles with improved ductility and low cost,and heat resistant martensitic steels with improved creep strength(10000650≥90 MPa).It can be expected that the new technology developed will remarkably improve the safety and reliability of steel products in service for infrastructures,automobiles and fossil power station in the future.
