从铝佐剂诞生至今,佐剂发挥着免疫调节、抗原递送的作用。新型人用疫苗佐剂陆续批准上市,以弥补疫苗免疫原性较弱的缺点。然而,人体免疫系统、病原体、佐剂之间的作用机制尚未完全清楚,佐剂诱导的自身免疫/炎性综合征(autoimmune/inflam...从铝佐剂诞生至今,佐剂发挥着免疫调节、抗原递送的作用。新型人用疫苗佐剂陆续批准上市,以弥补疫苗免疫原性较弱的缺点。然而,人体免疫系统、病原体、佐剂之间的作用机制尚未完全清楚,佐剂诱导的自身免疫/炎性综合征(autoimmune/inflammatory syndrome induced by adjuvants,ASIA)及相关不良反应,限制了人用疫苗佐剂的大量使用,这些均引起了人们对佐剂安全性的高度关注。现就7种已上市人用疫苗佐剂(铝佐剂、MF59、AS03、ISA51、病毒体佐剂、AS01及AS04)的作用机理、应用情况及其安全性问题作一阐述,为新型人用疫苗佐剂合理、可靠的分析与评价提供思路。展开更多
[ Objective] To clone the porcine interleukin-18（/L-18） cDNA and explore the immunological effectiveness of porcine IL-18 as an adjuvant of genetic vaccine. [ Method] The spleen lymphccytes were isolated from Henan ...[ Objective] To clone the porcine interleukin-18（/L-18） cDNA and explore the immunological effectiveness of porcine IL-18 as an adjuvant of genetic vaccine. [ Method] The spleen lymphccytes were isolated from Henan three-way cross-breeding pigs. According to the porcine IL-18 gene in GenBank, a pair of specific primers was designed. The full length cDNA of porcine IL-18 was amplified by RT-PCR. Subsequently, porcine IL-18 cDNA was cloned into pGEM-T vector and sequenced and analyzed. [ Result] The porcine IL-18 gene demonstrated an open reading frame of 579 bp encoding an inactive precursor protein with 192 amino acids. The precursor protein had no typical hydrophobic signal peptide and cleaved by interleukin-1 beta （IL-1β） converting enzyme（ICE） in caspase-1 splice site; the porcine mature protein had biological activity： After comparing with other porcine IL-18 genes, the nucleotide sequence homology was over 96% and the deduced amino acid homology was more than 98%. [ Conclusion] A full length procine IL-18 gene was gained. It lays the foundation for porcine IL-18 as an adjuvant of genetic vaccine.展开更多
文摘从铝佐剂诞生至今,佐剂发挥着免疫调节、抗原递送的作用。新型人用疫苗佐剂陆续批准上市,以弥补疫苗免疫原性较弱的缺点。然而,人体免疫系统、病原体、佐剂之间的作用机制尚未完全清楚,佐剂诱导的自身免疫/炎性综合征(autoimmune/inflammatory syndrome induced by adjuvants,ASIA)及相关不良反应,限制了人用疫苗佐剂的大量使用,这些均引起了人们对佐剂安全性的高度关注。现就7种已上市人用疫苗佐剂(铝佐剂、MF59、AS03、ISA51、病毒体佐剂、AS01及AS04)的作用机理、应用情况及其安全性问题作一阐述,为新型人用疫苗佐剂合理、可靠的分析与评价提供思路。
基金Supported by Project of Basic and Frontier Technology Research in Henan Province(072300430060 )Key Scientific and Technological Project of Henan Province(072102130023)~~
文摘[ Objective] To clone the porcine interleukin-18（/L-18） cDNA and explore the immunological effectiveness of porcine IL-18 as an adjuvant of genetic vaccine. [ Method] The spleen lymphccytes were isolated from Henan three-way cross-breeding pigs. According to the porcine IL-18 gene in GenBank, a pair of specific primers was designed. The full length cDNA of porcine IL-18 was amplified by RT-PCR. Subsequently, porcine IL-18 cDNA was cloned into pGEM-T vector and sequenced and analyzed. [ Result] The porcine IL-18 gene demonstrated an open reading frame of 579 bp encoding an inactive precursor protein with 192 amino acids. The precursor protein had no typical hydrophobic signal peptide and cleaved by interleukin-1 beta （IL-1β） converting enzyme（ICE） in caspase-1 splice site; the porcine mature protein had biological activity： After comparing with other porcine IL-18 genes, the nucleotide sequence homology was over 96% and the deduced amino acid homology was more than 98%. [ Conclusion] A full length procine IL-18 gene was gained. It lays the foundation for porcine IL-18 as an adjuvant of genetic vaccine.
