According to conventional theory, little genomic changes should occur in homozygous and stable amphiploids of the grass family, particularly those involving polyploid wheat as a parent. In the present study, however, ...According to conventional theory, little genomic changes should occur in homozygous and stable amphiploids of the grass family, particularly those involving polyploid wheat as a parent. In the present study, however, extensive genomic changes were detected in two octoploid partial amphiploids of common wheat (Triticum aestivum L.)_wheatgrass (Agropyron intermedium (Host) P.B.=Elytrigia intermedia (Host) Nevski=Thinopyrum intermedium (Host) Barkworth and Dewey), namely Zhong 3 and Zhong 5, by RFLP analysis using 10 low_copy, wheat chromosome_specific sequences and 33 representative homoeologous group_specific sequences as probes. Genomic changes involved loss of wheat hybridization fragment(s) and/or acquisition of new fragment(s). Uniformity of the RFLP patterns among 5 individual plants taken respectively from Zhong 3 and Zhong 5 in two successive generations, suggested that genomic changes probably had occurred in the early few generations after octoploid amphiploid formation, and remained essentially static thereafter. The highly similar RFLP patterns between Zhong 3 and Zhong 5, which had identical genomic constitution but differed from each other due to involvement of different wheat varieties as parents imply that genomic changes were probably not at random. Possible causes for the extensive and rapid genomic changes in the newly formed plant amphiploids, as well as their implications for polyploid genome evolution and breeding application are discussed.展开更多
An improved on-chip CMOS astable multivibrator is proposed, which overcomes the shortcomings of the traditional one that the signal duty-cycle is depending on model parameters, and generates stable clock signal with d...An improved on-chip CMOS astable multivibrator is proposed, which overcomes the shortcomings of the traditional one that the signal duty-cycle is depending on model parameters, and generates stable clock signal with duty-cycle equaling 50%. The latch-up effect has been prevented on the improved circuit. It is extremely important that all the excellent performances of the improved astable multivibrator have been achieved with a dynamic power consumption equaling its predecessor one. The advantage of the structure has been verified by SPICE simulation.展开更多
Recent work revealed that, in the genomes of polyploid wheat, there exists a class of low_copy and chromosome_specific sequences that are labile upon polyploid formation. This class of sequences was proposed to play ...Recent work revealed that, in the genomes of polyploid wheat, there exists a class of low_copy and chromosome_specific sequences that are labile upon polyploid formation. This class of sequences was proposed to play a critical role in the stabilization and establishment of nascent plant polyploids as new species. To further study this issue, five wheat chromosome 7B_specific sequences, isolated from common wheat (Triticum aestivum L.) by chromosome microdissection, were characterized. The sequences were studied by genomic Southern hybridizations on a collection of polyploid wheats and their diploid progenitors. Four sequences hybridized to all polyploid species, but at the diploid level to only species closely related to the B_genome of polyploid wheat. This indicates that these sequences originated with the divergence of the diploid species, and was then vertically transmitted to polyploids. One sequence hybridized to all species at both the diploid and polyploid levels, suggesting its elimination after the polyploid wheat formation. The hybridization of this sequence to two synthetic polyploid wheats indicated that sequence elimination is a rapid event and probably related to methylation status of the sequence. Based on the above results, we suggest that selective changes of low_copy sequences occur rapidly after polyploid formation, which may contribute to the differentiation of chromosomes in newly formed allopolyploid wheats.展开更多
The material T240_6 derived from SC 2 young embryo of the combination CA9211/RW15 (6D/6V alien substitution) was telosomic substitution line of 6VS identified by GISH (genomic in situ hybridization) analysis. The 6V...The material T240_6 derived from SC 2 young embryo of the combination CA9211/RW15 (6D/6V alien substitution) was telosomic substitution line of 6VS identified by GISH (genomic in situ hybridization) analysis. The 6VS was microdissected with a needle and transferred into a 0.5 mL Ep tube. In the 'single tube', all the subsequence steps were conducted. After two round of LA (Linker adaptor)_PCR amplification, the size of PCR bands ranged from 100 to 3 000 bp, with predominate bands 600-1 500 bp. The products were confirmed by Southern blotting analysis using Haynaldia villosa (L.) Schur. genomic DNA labeled with 32 P as probe. The PCR products were purified and ligated into clone vector-pGEM_T easy vector. Then, the plasmids were transformed into competence E. coli JM109 with cool CaCl 2. It was estimated that there were more than 17 000 white clones in the library. The size of insert fragments distributed from 100-1 500 bp, with average of 600 bp. Using H. villosa genomic DNA as probe, dot blotting results showed that 37% clones displayed strong and medium positive signals, and 63% clones had faint or no signals. It is demonstrated that there were about 37% repeat sequence clones and 67% single/unique sequence clones in the library. Eight H. villosa_specific clones were screened from the library, and two clones pHVMK22 and pHVMK134 were used for RFLP analysis and sequencing. Both of them were H. villosa specific clones. The pHVMK22 was a unique sequence clone, and the pHVMK134 was a repeat sequence clone. When the pHVMK22 was used as a probe for Southern hybridization, all the powdery mildew resistance materials showed a special band of 2 kb, while all the susceptible ones not. The pHVMK22 may be applied to detect the existence of Pm21.展开更多
Aim To design and implement a multi-agent cooperative problem solving expert system tool. Methods A blackboard system was adopted in the system as a data sharing and information exchanging center, to coordinate the co...Aim To design and implement a multi-agent cooperative problem solving expert system tool. Methods A blackboard system was adopted in the system as a data sharing and information exchanging center, to coordinate the complex cooperative problem solving. The system was developed in UNIX and MSWindows 95 mixed TCP/IP network environment. Results and Conclusion A prototype system of a multi-agent cooperative expert systems tool is implemented.The experiment demonstrates that the fundamental functions of a cooperative expert systems is realized.展开更多
China is by far the largest producer of tobacco products in the world. As a big tax source, tobacco industry brings about 10%of China's annual income.At present tobacco industry is under the administrative monopol...China is by far the largest producer of tobacco products in the world. As a big tax source, tobacco industry brings about 10%of China's annual income.At present tobacco industry is under the administrative monopoly management in China. The nation's policy and management mechanism greatly influence the competition structure of tobbacco industry. Due to the double character of tobacco, the country generally imposes a high tax and limits total product output in order to control the tobacco market. Therefore, the price signal of tobacco market is distorted and to a large extent the competition of the industry is far from a free one. The cigarette production is in a multi element competition style within the nation's planning system. In other words, administrative monopoly and over competition exist simultaneously in China's tobacco industry. The country predetermines the output quota of each cigarette manufacturing enterprise and the actual production outputs of these enterprises could be adjusted through the quota trades among them. The competition in tobacco industry is mainly represented in 3 modes, i.e.production output competition based on quota trades, value added competition in producing and marketing, and the niche market competition based on product classification.展开更多
An approach was proposed to specify the C4ISR capability of domain-specific modeling language.To confine the domain modeling within a standard architecture framework,formally a C4ISR capability meta-ontology was defin...An approach was proposed to specify the C4ISR capability of domain-specific modeling language.To confine the domain modeling within a standard architecture framework,formally a C4ISR capability meta-ontology was defined according to the meta-model of DoD Architecture Framework.The meta-ontology is used for extending UML Profile so that the domain experts can model the C4ISR domains using the C4ISR capability meta-concepts to define a domain-specific modeling language.The domain models can be then checked to guarantee the consistency and completeness through converting the UML models into the Description Logic ontology and making use of inference engine Pellet to verify the ontology.展开更多
In the 2013 release of the U.S. National Physical Education Standards the term "physically literate" replaced "physically educated". Un- fortunately, most discourse within the profession about the term physically ...In the 2013 release of the U.S. National Physical Education Standards the term "physically literate" replaced "physically educated". Un- fortunately, most discourse within the profession about the term physically literate occurred primarily after its adoption. While we agree with the spirit and intent of the term, we feel it is essential to discuss not only what has been potentially gained but also lost. In our paper, we illustrate the similarity of the terms physically educated and physically literate and essentially, from a definitional perspective, find little difference--but are these terms interchangeable? We provide a critical review of the standards and conclude that the change to physical literacy has produced a shift away from psychomotor outcomes to cognitive outcomes. Our concerns about this are many, but most importantly they are about the need to emphasize the "physical" in physical education (PE). It is our belief that the key to elevating the profession and maintaining and increasing support for PE is in its ability to promote and provide physical activity. Without physical activity and physical fitness as main outcomes, PE increases its vulnerability to extinction as a standard part of the U.S. K-12 education curriculum.展开更多
The main contents of this paper is a new trend of employment status and employment difficulties of professional sports graduates in Jilin, and analysis on Jilin Physical graduates' Internal Education Causes of employ...The main contents of this paper is a new trend of employment status and employment difficulties of professional sports graduates in Jilin, and analysis on Jilin Physical graduates' Internal Education Causes of employment difficulties ,and how to effectively promote employment of Jilin education and sports undergraduate and graduate and implement measures. In this paper, we use literature, interview survey method, to analyze five professional employment situations of the graduates (physical education major, professional sports training, human movement science, and social sports professional, traditional sports professional). From this perspective of the internal education, employment difficulties of professional sports graduates are these : the concept of employment is a direct factor behind their employment difficulties ; university entrepreneurship education are indirect factors behind Kyrgyzstan employment difficulties ; training model obsolete is the root cause of their difficulties in finding jobs; college enrollment in recent years is its employment difficulties catalyst. This article analyzes the employment situation relating to education, and proposed employment countenueasures which professional sports graduates adapt to, namely: to meet market demand, improve professional structural adjustment; establish the concept of occupation after the first job; establish their own businesses and Multiple Employment Awareness; create a "multi-skilltype" physical training model.展开更多
In traditional Chinese medicine(TCM),ophthalmic syndrome differentiation is an ophthalmology-specific method for identifying syndromes based on the“Five Orbiculi”theory.It was devised by Professor Qing-Hua PENG thro...In traditional Chinese medicine(TCM),ophthalmic syndrome differentiation is an ophthalmology-specific method for identifying syndromes based on the“Five Orbiculi”theory.It was devised by Professor Qing-Hua PENG through an unprecedented combination of syndrome element differentiation and ophthalmic clinical practices,based on the Clinical Terminology of Chinese Medical Diagnosis and Treatment-Syndromes of the National Standards of the People's Republic of China.This approach integrates an ophthalmic syndrome differentiation system with digital Chinese medicine(DCM),and proposes the extraction of syndrome elements of ophthalmic diseases from research on DCM.These elements are then quantified and organized to form a model of digital diagnosis and treatment specific to ophthalmology,which should help to achieve synergistic development of the ophthalmic syndrome differentiation system and DCM.展开更多
With the reform of the education system, the number of graduating students increases every year, and more and more severe graduate' s employment, while more and more companies are unable to recruit talent. Resulting ...With the reform of the education system, the number of graduating students increases every year, and more and more severe graduate' s employment, while more and more companies are unable to recruit talent. Resulting in difficult employment graduates there are many factors, the most important manifestations of contemporary graduates questioned the integrity of most enterprises, improve the status of today' s society difficult employment, increase the employment rate of graduates realize their own values of contemporary college students, meet the vital interests of the family, so that our talents and educational resources are fully utilized, so as to promote the healthy development of social harmony and stability as well as higher education. Social harmony and stability as well as for the healthy development of higher education, the vital interests of the students themselves and their families realize the value and so have certain theoretical and practical significance.展开更多
Wroclaw is mostly known for being a university town. In addition, the city is known in Poland for its sports history--basketball and football-and for many cultural events such as the Review of Stage Songs or African C...Wroclaw is mostly known for being a university town. In addition, the city is known in Poland for its sports history--basketball and football-and for many cultural events such as the Review of Stage Songs or African Culture Brave Festival. Because of the fact that Wroclaw is one of the largest and most important cities in Poland, it quickly becomes a subject of interest in new media, including the Internet, which becomes a field for community projects. This gives a tremendous opportunity to Wroclaw's youth who can participate in the life of Wroclaw, as well as by volunteering learn the basics of journalism and pursue their passions in an active and creative way. In perspective of those fact, the main aim of the planned paper is to describe evolution process of possibilities which Internet media gives to young people who would like to be a journalist. It is also a trial of analysis on how citizen journalism destroyed media voluntary youth work in Wroclaw, where in fact real citizen journalism was started by young people. What is more, paper's goal is to present methods of urban space documentation created by teenage journalists to show multicultural spectrum of Wroclaw.展开更多
文摘According to conventional theory, little genomic changes should occur in homozygous and stable amphiploids of the grass family, particularly those involving polyploid wheat as a parent. In the present study, however, extensive genomic changes were detected in two octoploid partial amphiploids of common wheat (Triticum aestivum L.)_wheatgrass (Agropyron intermedium (Host) P.B.=Elytrigia intermedia (Host) Nevski=Thinopyrum intermedium (Host) Barkworth and Dewey), namely Zhong 3 and Zhong 5, by RFLP analysis using 10 low_copy, wheat chromosome_specific sequences and 33 representative homoeologous group_specific sequences as probes. Genomic changes involved loss of wheat hybridization fragment(s) and/or acquisition of new fragment(s). Uniformity of the RFLP patterns among 5 individual plants taken respectively from Zhong 3 and Zhong 5 in two successive generations, suggested that genomic changes probably had occurred in the early few generations after octoploid amphiploid formation, and remained essentially static thereafter. The highly similar RFLP patterns between Zhong 3 and Zhong 5, which had identical genomic constitution but differed from each other due to involvement of different wheat varieties as parents imply that genomic changes were probably not at random. Possible causes for the extensive and rapid genomic changes in the newly formed plant amphiploids, as well as their implications for polyploid genome evolution and breeding application are discussed.
文摘An improved on-chip CMOS astable multivibrator is proposed, which overcomes the shortcomings of the traditional one that the signal duty-cycle is depending on model parameters, and generates stable clock signal with duty-cycle equaling 50%. The latch-up effect has been prevented on the improved circuit. It is extremely important that all the excellent performances of the improved astable multivibrator have been achieved with a dynamic power consumption equaling its predecessor one. The advantage of the structure has been verified by SPICE simulation.
文摘Recent work revealed that, in the genomes of polyploid wheat, there exists a class of low_copy and chromosome_specific sequences that are labile upon polyploid formation. This class of sequences was proposed to play a critical role in the stabilization and establishment of nascent plant polyploids as new species. To further study this issue, five wheat chromosome 7B_specific sequences, isolated from common wheat (Triticum aestivum L.) by chromosome microdissection, were characterized. The sequences were studied by genomic Southern hybridizations on a collection of polyploid wheats and their diploid progenitors. Four sequences hybridized to all polyploid species, but at the diploid level to only species closely related to the B_genome of polyploid wheat. This indicates that these sequences originated with the divergence of the diploid species, and was then vertically transmitted to polyploids. One sequence hybridized to all species at both the diploid and polyploid levels, suggesting its elimination after the polyploid wheat formation. The hybridization of this sequence to two synthetic polyploid wheats indicated that sequence elimination is a rapid event and probably related to methylation status of the sequence. Based on the above results, we suggest that selective changes of low_copy sequences occur rapidly after polyploid formation, which may contribute to the differentiation of chromosomes in newly formed allopolyploid wheats.
基金国家"8 6 3"计划资助项目 (Z 17 0 4 0 1) 国家转基因植物研究与产业化资助项目 (J0 0 A 0 0 2 )~~
文摘The material T240_6 derived from SC 2 young embryo of the combination CA9211/RW15 (6D/6V alien substitution) was telosomic substitution line of 6VS identified by GISH (genomic in situ hybridization) analysis. The 6VS was microdissected with a needle and transferred into a 0.5 mL Ep tube. In the 'single tube', all the subsequence steps were conducted. After two round of LA (Linker adaptor)_PCR amplification, the size of PCR bands ranged from 100 to 3 000 bp, with predominate bands 600-1 500 bp. The products were confirmed by Southern blotting analysis using Haynaldia villosa (L.) Schur. genomic DNA labeled with 32 P as probe. The PCR products were purified and ligated into clone vector-pGEM_T easy vector. Then, the plasmids were transformed into competence E. coli JM109 with cool CaCl 2. It was estimated that there were more than 17 000 white clones in the library. The size of insert fragments distributed from 100-1 500 bp, with average of 600 bp. Using H. villosa genomic DNA as probe, dot blotting results showed that 37% clones displayed strong and medium positive signals, and 63% clones had faint or no signals. It is demonstrated that there were about 37% repeat sequence clones and 67% single/unique sequence clones in the library. Eight H. villosa_specific clones were screened from the library, and two clones pHVMK22 and pHVMK134 were used for RFLP analysis and sequencing. Both of them were H. villosa specific clones. The pHVMK22 was a unique sequence clone, and the pHVMK134 was a repeat sequence clone. When the pHVMK22 was used as a probe for Southern hybridization, all the powdery mildew resistance materials showed a special band of 2 kb, while all the susceptible ones not. The pHVMK22 may be applied to detect the existence of Pm21.
文摘Aim To design and implement a multi-agent cooperative problem solving expert system tool. Methods A blackboard system was adopted in the system as a data sharing and information exchanging center, to coordinate the complex cooperative problem solving. The system was developed in UNIX and MSWindows 95 mixed TCP/IP network environment. Results and Conclusion A prototype system of a multi-agent cooperative expert systems tool is implemented.The experiment demonstrates that the fundamental functions of a cooperative expert systems is realized.
文摘China is by far the largest producer of tobacco products in the world. As a big tax source, tobacco industry brings about 10%of China's annual income.At present tobacco industry is under the administrative monopoly management in China. The nation's policy and management mechanism greatly influence the competition structure of tobbacco industry. Due to the double character of tobacco, the country generally imposes a high tax and limits total product output in order to control the tobacco market. Therefore, the price signal of tobacco market is distorted and to a large extent the competition of the industry is far from a free one. The cigarette production is in a multi element competition style within the nation's planning system. In other words, administrative monopoly and over competition exist simultaneously in China's tobacco industry. The country predetermines the output quota of each cigarette manufacturing enterprise and the actual production outputs of these enterprises could be adjusted through the quota trades among them. The competition in tobacco industry is mainly represented in 3 modes, i.e.production output competition based on quota trades, value added competition in producing and marketing, and the niche market competition based on product classification.
基金Project(2007AA01Z126) supported by the National High Technology Research and Development Program of ChinaProject(51306010202) supported by the National Defense Advance Research Program of China
文摘An approach was proposed to specify the C4ISR capability of domain-specific modeling language.To confine the domain modeling within a standard architecture framework,formally a C4ISR capability meta-ontology was defined according to the meta-model of DoD Architecture Framework.The meta-ontology is used for extending UML Profile so that the domain experts can model the C4ISR domains using the C4ISR capability meta-concepts to define a domain-specific modeling language.The domain models can be then checked to guarantee the consistency and completeness through converting the UML models into the Description Logic ontology and making use of inference engine Pellet to verify the ontology.
文摘In the 2013 release of the U.S. National Physical Education Standards the term "physically literate" replaced "physically educated". Un- fortunately, most discourse within the profession about the term physically literate occurred primarily after its adoption. While we agree with the spirit and intent of the term, we feel it is essential to discuss not only what has been potentially gained but also lost. In our paper, we illustrate the similarity of the terms physically educated and physically literate and essentially, from a definitional perspective, find little difference--but are these terms interchangeable? We provide a critical review of the standards and conclude that the change to physical literacy has produced a shift away from psychomotor outcomes to cognitive outcomes. Our concerns about this are many, but most importantly they are about the need to emphasize the "physical" in physical education (PE). It is our belief that the key to elevating the profession and maintaining and increasing support for PE is in its ability to promote and provide physical activity. Without physical activity and physical fitness as main outcomes, PE increases its vulnerability to extinction as a standard part of the U.S. K-12 education curriculum.
文摘The main contents of this paper is a new trend of employment status and employment difficulties of professional sports graduates in Jilin, and analysis on Jilin Physical graduates' Internal Education Causes of employment difficulties ,and how to effectively promote employment of Jilin education and sports undergraduate and graduate and implement measures. In this paper, we use literature, interview survey method, to analyze five professional employment situations of the graduates (physical education major, professional sports training, human movement science, and social sports professional, traditional sports professional). From this perspective of the internal education, employment difficulties of professional sports graduates are these : the concept of employment is a direct factor behind their employment difficulties ; university entrepreneurship education are indirect factors behind Kyrgyzstan employment difficulties ; training model obsolete is the root cause of their difficulties in finding jobs; college enrollment in recent years is its employment difficulties catalyst. This article analyzes the employment situation relating to education, and proposed employment countenueasures which professional sports graduates adapt to, namely: to meet market demand, improve professional structural adjustment; establish the concept of occupation after the first job; establish their own businesses and Multiple Employment Awareness; create a "multi-skilltype" physical training model.
文摘In traditional Chinese medicine(TCM),ophthalmic syndrome differentiation is an ophthalmology-specific method for identifying syndromes based on the“Five Orbiculi”theory.It was devised by Professor Qing-Hua PENG through an unprecedented combination of syndrome element differentiation and ophthalmic clinical practices,based on the Clinical Terminology of Chinese Medical Diagnosis and Treatment-Syndromes of the National Standards of the People's Republic of China.This approach integrates an ophthalmic syndrome differentiation system with digital Chinese medicine(DCM),and proposes the extraction of syndrome elements of ophthalmic diseases from research on DCM.These elements are then quantified and organized to form a model of digital diagnosis and treatment specific to ophthalmology,which should help to achieve synergistic development of the ophthalmic syndrome differentiation system and DCM.
文摘With the reform of the education system, the number of graduating students increases every year, and more and more severe graduate' s employment, while more and more companies are unable to recruit talent. Resulting in difficult employment graduates there are many factors, the most important manifestations of contemporary graduates questioned the integrity of most enterprises, improve the status of today' s society difficult employment, increase the employment rate of graduates realize their own values of contemporary college students, meet the vital interests of the family, so that our talents and educational resources are fully utilized, so as to promote the healthy development of social harmony and stability as well as higher education. Social harmony and stability as well as for the healthy development of higher education, the vital interests of the students themselves and their families realize the value and so have certain theoretical and practical significance.
文摘Wroclaw is mostly known for being a university town. In addition, the city is known in Poland for its sports history--basketball and football-and for many cultural events such as the Review of Stage Songs or African Culture Brave Festival. Because of the fact that Wroclaw is one of the largest and most important cities in Poland, it quickly becomes a subject of interest in new media, including the Internet, which becomes a field for community projects. This gives a tremendous opportunity to Wroclaw's youth who can participate in the life of Wroclaw, as well as by volunteering learn the basics of journalism and pursue their passions in an active and creative way. In perspective of those fact, the main aim of the planned paper is to describe evolution process of possibilities which Internet media gives to young people who would like to be a journalist. It is also a trial of analysis on how citizen journalism destroyed media voluntary youth work in Wroclaw, where in fact real citizen journalism was started by young people. What is more, paper's goal is to present methods of urban space documentation created by teenage journalists to show multicultural spectrum of Wroclaw.