Water channels or aquaporins are the main pathways of water transport. Both the existence and function of aquaporins in die guard cells of Vicia faba L. were investigated both by using RD28 cDNA and RD28 antibody as p...Water channels or aquaporins are the main pathways of water transport. Both the existence and function of aquaporins in die guard cells of Vicia faba L. were investigated both by using RD28 cDNA and RD28 antibody as probes, and by controlling stomatal movement as a parameter combined with antibody and inhibitor of aquaporins respectively. The results revealed that RD28 mRNA, encoding a plasma membrane aquaporin, expressed in ale mesophyll cells and vascular tissues of V. faba, especially in guard cells. And the location of RD28-like proteins was mainly on plasma membrane of guard cells. The addition of 25 mumol/L HgCl2, an aquaporin blocker, and antibody of RD28 as well, greatly suppressed the stomatal opening or guardcell protoplast swelling induced by fusicoccin and light, and closing induced by abscisic acid. However, 5 mmol/L, beta-mercaptoethanol, a reverse reagent of aquaporin blocker, reversed the inhibitory effect of HgCl2 Pretreatment oil stomatal opening ( i.e., HgCl2 was removed after HgCl2 pretreatment for 10 min). The results suggest that the aquaporins in V. faba are associated with stomatal movement.展开更多
A dead reckoning system for a wheeled mobile robot was designed, and the method for robot’s pose estimation in the 3D environments was presented on the basis of its rigid-body kinematic equations. After analyzing the...A dead reckoning system for a wheeled mobile robot was designed, and the method for robot’s pose estimation in the 3D environments was presented on the basis of its rigid-body kinematic equations. After analyzing the locomotion architecture of mobile robot and the principle of proprioceptive sensors, the kinematics model of mobile robot was built to realize the relative localization. Considering that the research on dead reckoning of mobile robot was confined to the 2 dimensional planes, the locomotion of mobile robot in the 3 coordinate axis direction was thought over in order to estimate its pose on uneven terrain. Because the computing method in a plane is rather mature, the calculation in height direction is emphatically represented as a key issue. With experimental results obtained by simulation program and robot platform, the position of mobile robot can be reliably estimated and the localization precision can be effectively improved, so the effectiveness of this dead reckoning system is demonstrated.展开更多
The couple effect of soil displacement and axial load on the single inclined pile in cases of surcharge load and uniform soil movement is discussed in detail with the methods of full-scale field tests and finite eleme...The couple effect of soil displacement and axial load on the single inclined pile in cases of surcharge load and uniform soil movement is discussed in detail with the methods of full-scale field tests and finite element method. Parametric analyses including the degree of inclination and the distance between soil and pile are carried out herein. When the displacement of soil on the left side and right side of a pile is identical, deformation of a vertical pile and an inclined pile is highly close in both cases of surcharge load and uniform soil movement. When the couple effect of soil displacement and axial load occurs, settlement of an inclined pile is greater than that of a vertical pile under the same axial load, and bearing capacity of an inclined pile is smaller than that of a vertical pile. This is quite different from the case when the inclined pile is not affected by soil displacement. For inclined piles, P-Δ effect of axial load would lead to a large increase in bending moment, however, for the vertical pile, P-Δ effect of axial load can be neglected. Although the direction of inclination of piles is reverse, deformation of piles caused by uniform soil movement is totally the same. For the inclined piles discussed herein, bending moment(-8 m to-17 m under the ground) relies heavily on uniform soil movement and does not change during the process of applying axial load. When the thickness of soil is less than the pile length, the greater the thickness of soil, the larger the bending moment at lower part of the inclined pile. When the thickness of soil is larger than the pile length, bending moment at lower part of the inclined pile is zero.展开更多
Although conventional suturing techniques are commonly used in assisting wound closure,they do pose limited conduciveness and may lead to secondary injury to wound tissues.Inspired by marine organism mussels,we design...Although conventional suturing techniques are commonly used in assisting wound closure,they do pose limited conduciveness and may lead to secondary injury to wound tissues.Inspired by marine organism mussels,we designed and manufactured a bio-inspired hydrogel-based bandage with tough wet tissue adhesion to substitute traditional surgical suture,accelerate wound healing and avoid infection.Poly(γ-glutamic acid)was modified with 3,4-dihydroxyphenylalanine and glycidyl methacylate,then introduced into the acrylic acid-co-acrylamide hydrogel matrix with robust mechanical properties.The hydrogel bandage showed strong chemical linkage adhesion(70±2.1 kPa),which is 2.8 times that of commercial tissue adhesive fibrin glue(25±2.2 kPa).The hydrogel bandage can not only maintain the self-stability,but is also capable of self-tuning adhesive strength in the range of 14-70 kPa to achieve different adhesion effects by tuning constituent ratio.The bandage has desirable compression properties(0.7±0.11 MPa)and tensile elongation(about 25 times),which ensures its resistance to damages,especially in joint spaces.Secondly,the bandage was endowed with antioxidant and endogenous broad-spectrum antibacterial properties with its catechol structure.Results also demonstrated excellent cell compatibility and blood compatibility,certifying its eligible biological safety profile.In a rat full-thickness cutaneous deficiency model,we can clearly observe that the bandage possesses the ability to promote wound healing(only need 6 days).Above all,this research provides a new strategy for the emergency treatment of liver hemostasis and myocardial repair during disaster rescue.展开更多
The motion of small bacteria consists of two phases:relatively long runs alternate with intermittent stops,back-ups,or tumbles,depending on the species.In polar monotrichous bacteria,the flagellum is anchored at the c...The motion of small bacteria consists of two phases:relatively long runs alternate with intermittent stops,back-ups,or tumbles,depending on the species.In polar monotrichous bacteria,the flagellum is anchored at the cell pole inherited from the parent generation(old pole) and is surrounded by a chemoreceptor cluster.During forward swimming,the leading pole is always the pole recently formed in cell division(new pole).The flagella of the peritrichous bacterium Escherichia coli often form a bundle behind the old pole.Its cell orientation and receptor positioning during runs generally mimic that of monotrichous bacteria.When encountering a solid surface,peritrichous bacteria exhibit a circular motion with the leading pole dipping downward.Some polar monotrichous bacteria also perform circular motion near solid boundaries,but during back-ups.In this case,the leading pole points upward.Very little is known about behavior near milieu-air interfaces.Biophysical simulations have revealed some of the mechanisms underlying these phenomena,but leave many questions unanswered.Combining biophysics with molecular techniques will certainly advance our understanding of bacterial locomotion.展开更多
文摘Water channels or aquaporins are the main pathways of water transport. Both the existence and function of aquaporins in die guard cells of Vicia faba L. were investigated both by using RD28 cDNA and RD28 antibody as probes, and by controlling stomatal movement as a parameter combined with antibody and inhibitor of aquaporins respectively. The results revealed that RD28 mRNA, encoding a plasma membrane aquaporin, expressed in ale mesophyll cells and vascular tissues of V. faba, especially in guard cells. And the location of RD28-like proteins was mainly on plasma membrane of guard cells. The addition of 25 mumol/L HgCl2, an aquaporin blocker, and antibody of RD28 as well, greatly suppressed the stomatal opening or guardcell protoplast swelling induced by fusicoccin and light, and closing induced by abscisic acid. However, 5 mmol/L, beta-mercaptoethanol, a reverse reagent of aquaporin blocker, reversed the inhibitory effect of HgCl2 Pretreatment oil stomatal opening ( i.e., HgCl2 was removed after HgCl2 pretreatment for 10 min). The results suggest that the aquaporins in V. faba are associated with stomatal movement.
文摘目的探讨人工全膝关节置换术(total knee artroplasty,TKA)中采用运动体位下间断缝合手术切口的疗效。方法选取2015年7月—2016年7月收治的84例拟行TKA的膝关节骨关节炎患者,随机分为对照组及观察组,每组42例。两组患者性别、年龄、侧别、体质量指数、骨关节炎分级等一般资料比较,差异均无统计学意义(P>0.05)。术中对照组于膝关节屈曲位间断缝合切口,观察组于运动体位间断缝合切口;两组术前和术后处理方法一致。比较两组切口长度、缝合时间、曲马多使用量、术中出血量、拆线时间、住院时间、术后并发症发生率、切口愈合评分(HWES)、患者切口自评满意度评分(Liktert评分);手术前后采用疼痛视觉模拟评分(VAS)评价疼痛程度,美国特种外科医院(HSS)评分和关节活动度(range of motion,ROM)评估膝关节功能。结果两组切口长度、术后并发症发生率、HWES评分、拆线时间及住院时间比较,差异无统计学意义(P>0.05);对照组缝合时间、术中出血量和Likter评分明显低于观察组,但术后曲马多使用量明显高于观察组,比较差异均有统计意义(P<0.05)。两组患者均获随访,对照组随访时间为12~24个月,平均14.7个月;观察组为12~23个月,平均15.3个月。两组患者术前及术后第1天睡觉前、拆线时VAS评分比较,差异均无统计学意义(P>0.05);观察组术后第1天屈伸功能锻炼时VAS评分明显低于对照组(P<0.05)。两组术前以及术后12个月时HSS评分、ROM比较,差异无统计学意义(P>0.05);观察组患者出院时以及术后1、3、6个月时以上两指标均优于对照组(P<0.05)。结论与膝关节屈曲位间断缝合相比,TKA术中采用运动体位下间断缝合切口,患者术后疼痛程度轻、切口愈合好、早期可获得较满意关节功能,但两者远期疗效无明显差异。
基金Project(60234030) supported by the National Natural Science Foundation of China
文摘A dead reckoning system for a wheeled mobile robot was designed, and the method for robot’s pose estimation in the 3D environments was presented on the basis of its rigid-body kinematic equations. After analyzing the locomotion architecture of mobile robot and the principle of proprioceptive sensors, the kinematics model of mobile robot was built to realize the relative localization. Considering that the research on dead reckoning of mobile robot was confined to the 2 dimensional planes, the locomotion of mobile robot in the 3 coordinate axis direction was thought over in order to estimate its pose on uneven terrain. Because the computing method in a plane is rather mature, the calculation in height direction is emphatically represented as a key issue. With experimental results obtained by simulation program and robot platform, the position of mobile robot can be reliably estimated and the localization precision can be effectively improved, so the effectiveness of this dead reckoning system is demonstrated.
基金Project(51208071)supported by the National Natural Science Foundation of ChinaProject(2010CB732106)supported by the National Basic Research Program of China
文摘The couple effect of soil displacement and axial load on the single inclined pile in cases of surcharge load and uniform soil movement is discussed in detail with the methods of full-scale field tests and finite element method. Parametric analyses including the degree of inclination and the distance between soil and pile are carried out herein. When the displacement of soil on the left side and right side of a pile is identical, deformation of a vertical pile and an inclined pile is highly close in both cases of surcharge load and uniform soil movement. When the couple effect of soil displacement and axial load occurs, settlement of an inclined pile is greater than that of a vertical pile under the same axial load, and bearing capacity of an inclined pile is smaller than that of a vertical pile. This is quite different from the case when the inclined pile is not affected by soil displacement. For inclined piles, P-Δ effect of axial load would lead to a large increase in bending moment, however, for the vertical pile, P-Δ effect of axial load can be neglected. Although the direction of inclination of piles is reverse, deformation of piles caused by uniform soil movement is totally the same. For the inclined piles discussed herein, bending moment(-8 m to-17 m under the ground) relies heavily on uniform soil movement and does not change during the process of applying axial load. When the thickness of soil is less than the pile length, the greater the thickness of soil, the larger the bending moment at lower part of the inclined pile. When the thickness of soil is larger than the pile length, bending moment at lower part of the inclined pile is zero.
基金supported by the National Natural Science Foundation of China(31771049)the Foundation of Key R&D Project of Jiangsu Province(BE2018731)+3 种基金the Research Foundation of State Key Laboratory of Materials-Oriented Chemical Engineering(ZK201806,KL18-06 and ZK201606)the Six Talent Peaks Project of Jiangsu Province(SWYY-046)the Natural Science Foundation of Jiangsu Province(BK20200682)the Postgraduate Research&Practice Innovation Program of Jiangsu Province(SJCX20_0408).
文摘Although conventional suturing techniques are commonly used in assisting wound closure,they do pose limited conduciveness and may lead to secondary injury to wound tissues.Inspired by marine organism mussels,we designed and manufactured a bio-inspired hydrogel-based bandage with tough wet tissue adhesion to substitute traditional surgical suture,accelerate wound healing and avoid infection.Poly(γ-glutamic acid)was modified with 3,4-dihydroxyphenylalanine and glycidyl methacylate,then introduced into the acrylic acid-co-acrylamide hydrogel matrix with robust mechanical properties.The hydrogel bandage showed strong chemical linkage adhesion(70±2.1 kPa),which is 2.8 times that of commercial tissue adhesive fibrin glue(25±2.2 kPa).The hydrogel bandage can not only maintain the self-stability,but is also capable of self-tuning adhesive strength in the range of 14-70 kPa to achieve different adhesion effects by tuning constituent ratio.The bandage has desirable compression properties(0.7±0.11 MPa)and tensile elongation(about 25 times),which ensures its resistance to damages,especially in joint spaces.Secondly,the bandage was endowed with antioxidant and endogenous broad-spectrum antibacterial properties with its catechol structure.Results also demonstrated excellent cell compatibility and blood compatibility,certifying its eligible biological safety profile.In a rat full-thickness cutaneous deficiency model,we can clearly observe that the bandage possesses the ability to promote wound healing(only need 6 days).Above all,this research provides a new strategy for the emergency treatment of liver hemostasis and myocardial repair during disaster rescue.
文摘The motion of small bacteria consists of two phases:relatively long runs alternate with intermittent stops,back-ups,or tumbles,depending on the species.In polar monotrichous bacteria,the flagellum is anchored at the cell pole inherited from the parent generation(old pole) and is surrounded by a chemoreceptor cluster.During forward swimming,the leading pole is always the pole recently formed in cell division(new pole).The flagella of the peritrichous bacterium Escherichia coli often form a bundle behind the old pole.Its cell orientation and receptor positioning during runs generally mimic that of monotrichous bacteria.When encountering a solid surface,peritrichous bacteria exhibit a circular motion with the leading pole dipping downward.Some polar monotrichous bacteria also perform circular motion near solid boundaries,but during back-ups.In this case,the leading pole points upward.Very little is known about behavior near milieu-air interfaces.Biophysical simulations have revealed some of the mechanisms underlying these phenomena,but leave many questions unanswered.Combining biophysics with molecular techniques will certainly advance our understanding of bacterial locomotion.